Didemnin biosynthetic gene cluster in tistrella mobilis

ABSTRACT

A novel  Tistrella mobilis  strain having Accession Deposit Number NRRL B-50531 is provided. A method of producing a didemnin precursor, didemnin or didemnin derivative by using the  Tistrella mobilis  strain, and the therapeutic composition comprising at least one didemnin or didemnin derivative produced from the strain or modified strain thereof are also provided.

This application claims priority to U.S. Provisional Patent ApplicationSer. No. 61/537,416, filed Sep. 21, 2011, which application isincorporated by reference herein in its entirety.

TECHNICAL FIELD

The present invention generally concerns the fields of cell biology,molecular biology, bacteriology, and medicine. In particular aspects,the invention concerns direct or indirect production of anti-cancercompounds in bacteria.

BACKGROUND OF THE INVENTION

The didemnins (FIG. 1) are a group of cyclic depsipeptides withextraordinary biological activities, including antitumor, antivirus andimmunosuppressive activities (Vera and Joullié, 2002; Rawat et al.,2006). Rinehart and his coworkers (1981) initially isolated didemnins A,B and C from a Caribbean tunicate of the family Didemnidae in 1981.Since then, more than 20 other didemnins have been isolated fromtunicates collected from different geographical locations. Owing to itspotent antitumor activity, didemnin B was the first marine naturalproduct selected to enter clinical trials. Although didemnin B failed inmid-1990s, a closely related didemnin compound-dehydrodidemnin B(Aplidin) is currently in human phase II clinic trials for solid andhaematological malignant neoplasias like T cell lymphoma andmyelofibrosis and in phase III clinical trials for multiple myeloma(Soto-Matos et al., 2011). Until now, didemnins are exclusively isolatedfrom the eukaryotic marine tunicates. However, since cyclic peptides areusually synthesized by non-ribosomal peptide synthetases (NRPS) frommicroorganisms, a microorganism associated with the tunicates would be auseful source for producing didemnins. Until the present invention,careful scrutiny of tunicate symbiotic microorganisms has not beensuccessful to locate such a microorganism and chemical synthesis remainsto be the only route to obtain sufficient amount of didemnins. Thechemical reactions to synthesize didemnin A, the simplest compound ofthe didemnin family, are too complicated to be finished within 10 steps(Jou et al, 1997), and consequently the yield is undoubtedly very low.Therefore, the discovery of didemnin biosynthetic gene cluster from amicrobe will be beneficial for producing didemnins more economically.More importantly, novel didemnin analogues can be generated throughgenetic engineering of the biosynthetic pathways and be investigated fortheir biological activities, which may in turn provide more drug leads.

BRIEF SUMMARY OF THE INVENTION

The present invention is directed to a system, method, and compositionsrelated to production of anti-cancer compounds or precursors orderivatives thereto from a bacterium. In particular aspects, thebacterium is from the Tistrella genus, and in specific aspects thebacterium is Tistrella mobilis.

This invention relates to the production of didemnins or didemninderivatives through the use of novel didemnin-producing bacterium and/ordidemnins or didemnin derivatives produced by these species; it alsorelates to polynucleotide and amino acids from the novel bacteriumproducing the didemnins. Furthermore, the invention relates totherapeutic compositions containing the didemnins and to uses of thetherapeutic compositions.

It is therefore an object of the present invention to provide novelspecies of didemnin-producing Tistrella bacteria. It is also an objectof the invention to provide a novel Tistrella mobilis bacterium or oneor more strains thereof. In specific cases, the novel didemnin-producingTistrella mobilis bacterium was deposited on Jul. 27, 2011 as AccessionNo. NRRL B-50531 with the depository Agricultural Research ServiceCulture Collection, National Center for Agricultural UtilizationResearch Agricultural Research Service, U.S. Department of Agriculture,1815 North University Street, Peoria, Ill. 61604, U.S.A.

An isolated or biologically pure culture of the bacterium is encompassedin the invention. A further object of the present invention is toprovide didemnins, didemnin precursors, and/or didemnin derivativesproduced by novel strains of Tistrella. In additional aspects of thepresent invention, there are novel amino acid and polynucleotidesequences of Tistrella mobilis, and exemplary sequences include SEQ IDNOS:10-61.

Any Tistrella species may be utilized in the invention. In specificembodiments, the following bacteria are utilized: Tistrella mobilis (Shiet al., 2002); Tistrella bauzanensis (Zhang et al., 2010), Tistrella sp.BZ78, Tistrella sp. D1-34, Tistrella sp. D1-36, Tistrella sp. D6-30,Tistrella sp. f-1-2, Tistrella sp. JW16.1a, Tistrella sp. MARC2PPND,Tistrella sp. PhS5A, Tistrella sp. S67-5, Tistrella sp. S73-3, andTistrella sp. Zp5.

Specific embodiments of the invention include the isolation of didemninB and nordidemnin B from a Gram-negative marine-derived bacteriumTistrella mobilis. The complete genome sequence of this bacteriumrevealed the biosynthetic gene cluster responsible for the didemninsynthesis. Bioinformatic analysis was used to predict the function ofthe genes in the cluster. Culture conditions were optimized in order toincrease the yield of the target didemnin compounds.

In particular aspects, there is a process for producing a didemnin,which comprises the steps of: a) culturing at least one Tistrella strainin growth-supporting nutrient medium capable of promoting growth andreproduction of said bacteria, wherein said culturing is effected for atime sufficient to allow production of a didemnin; and b) recovering thedidemnin from said bacteria or medium of step a).

One embodiment of the present invention is to provide a plurality ofbacteria for the mass production of a didemnin or precursor orderivative thereof.

A particular embodiment of the present invention is to provide a novelprocess for the production of didemnins, didemnin precursors, ordidemnin derivatives from bacteria. The industrial application of thisprocess would provide renewable sources of didemnins, didemninprecursors, or didemnin derivatives for the pharmaceutical industry. Incertain aspects, there is a biotransformation process in whichbacteria-derived didemnins, didemnin precursors, or didemnin derivativesare converted into substances that are useful as therapeutic compoundsor for the production of other therapeutic compounds. In certain aspectsof the invention, didemnin B and/or nordidemnin B (for example) areproduced endogenously by Tistrella mobilis and derivatives are madetherefrom one or the other; however, in other cases other didemnins areproduced endogenously by other Tistrella bacteria.

Aspects of the invention include methods and compositions whereinbacteria capable of producing didemnin precursors or derivatives,wherein reagents are added to their culture medium to effect productionof a didemnin or the didemnin derivatives.

In accordance with the present invention there is also provided aprocess for improving production of didemnins, didemnin precursors, ordidemnin derivatives in bacteria comprising the steps of a) culturingTistrella bacteria in the presence of a mutagenic agent for a period oftime sufficient to allow mutagenesis; and b) selecting said mutants by achange of the phenotype that results in an increased production ofdidemnins, didemnin precursors, or didemnin derivatives. The mutagenicagent may be a chemical agent, such as daunorubicin andnitrosoguanidine; a physical agent, such as gamma radiation orultraviolet radiation; or a biological agent, such as a transposon, forexample. Exemplary modifications include to the side chain region, tothe hip-isostatine region, to the tetrapeptide region, and/or to themacrocyclic backbone.

In certain embodiments, there is provided a process for improvingbiotransformation of didemnins into didemnin derivative-producingbacteria comprising the steps of a) culturing bacteria in the presenceof a mutagenic agent for a time sufficient to allow mutagenesis; and b)selecting said mutants by a change of the phenotype that results in anincreased biotransformation of didemnins into didemninderivative-producing bacteria.

In some aspects of the invention, an anti-cancer compound is producedfrom Tistrella bacteria. The compound may be isolated directly from thebacteria, or another compound may be isolated from the bacteria fromwhich the anti-cancer compound is then synthesized, either directly orindirectly through one or more other compounds.

Embodiments of the invention include methods and compositions regardingfermentation to produce didemnins and their derivative compounds. Someembodiments include the gene cluster in Tistrella for producingdidemnins. Although in specific cases the didemnin gene cluster includesTistrella didA, didB, didC, didD, didE, didF, didG, didH, and didI;however, in particular embodiments the didemnin gene cluster includesone or more of Tistrella didA, didB, didC, didD, didE, didF, didG, didH,and didI. In at least certain aspects, one or more of the didemnin genecluster may be transformed into bacteria from another genus, such asEscherichia; in particular, one or more of the didemnin gene clustermembers may be transferred into E. coli for the production of a didemninor didemnin derivative from E. coli, either directly or indirectly.

In particular aspects of the invention, there is included production ofdidemnins, didemnin precursors, or didemnin derivatives by the Tistrellamobilis JAM 14872T (Shi et al., 2002). Tistrella mobilis JAM 14872T wascultured and extracted by the same way as the Tistrella mobilis straindescribed herein, and the UPLC-HRMS profile shows that this type strainalso produces didemnin B and nordidemnin B.

Anti-cancer compounds of the present invention may be useful for anytype of cancer, including at least the following: breast, lung,prostate, colon, pancreatic, blood, brain, liver, spleen, esophageal,ovarian, cervical, kidney, thyroid, rectal, bone, gall bladder, stomach,and so forth. The invention may employed for any type of mammal,including humans, dogs, cats, horses, pigs, sheep, and goats.

In alternative embodiments, the compositions of the invention relate toanti-viral and/or immunosuppressive compounds.

In some aspects of the invention, one or more polynucleotides inTistrella (such as one of plasmids 1, 2, 3, or 4 of Tistrella mobilis,for example) are able to conjugate with other bacteria. In specificcases, the plasmid transfers to E. coli or another bacterium.

In an embodiment of the invention, there is an isolated Tistrellamobilis bacterium having Accession Deposit Number NRRL B-50531 with thedepository Agricultural Research Service Culture Collection NationalCenter for Agricultural Utilization Research Agricultural ResearchService, U.S. Department of Agriculture. In some embodiments, there isan isolated polynucleotide selected from the group consisting of SEQ IDNO:10-25, 42-51, 62-66, and a mixture thereof. In particularembodiments, there is an isolated polypeptide selected from the groupconsisting of SEQ ID NO:26-41, 52-61, and a mixture thereof.

In specific embodiments, a bacterium comprises at least one geneticmodification compared to wild-type, and in certain aspects the geneticmodification is in a gene in the didemnin gene cluster. In specificcases, at least one genetic modification is in a condensation,adenylation, thiolation, ketoreductase, ketosynthase, methyltransferase,or thioesterase domain of a gene in the didemnin gene cluster. Incertain embodiments, at least one genetic modification is in theketoreductase or adenylation domains.

In one embodiment of the invention, there is a method of producing adidemnin precursor, didemnin, or didemnin derivative, comprising thesteps of a) culturing a host cell harboring bacterial didemnin synthesisgenes; and b) recovering said didemnin precursor, didemnin, or didemninderivative from said host cell. In some cases, the host cell is furtherdefined as a Tistrella bacterium, a genetically modified Tistrellabacterium compared to wild-type, or E. coli. The Tistrella bacterium maybe Tistrella mobilis, in some cases. In particular aspects, the didemninis selected from the group consisting of didemnin A, didemnin B,didemnin C, didemnin D, didemnin E, didemnin G, didemnin X, didemnin Y,nordidemnin, or a combination thereof. In some cases, the method furthercomprises modifying the recovered didemnin precursor, didemnin, ordidemnin derivative.

In an embodiment of the invention, there is a therapeutic compositioncomprising in a suitable carrier: at least one isolated didemninproduced by a culture comprising bacteria having Accession DepositNumber NRRL B-50531 with the depository Agricultural Research ServiceCulture Collection National Center for Agricultural Utilization ResearchAgricultural Research Service, U.S. Department of Agriculture; at leastone didemnin or didemnin derivative produced from a modified bacteria ofthe bacterial strain having Accession Deposit Number NRRL B-50531 withthe depository Agricultural Research Service Culture Collection NationalCenter for Agricultural Utilization Research Agricultural ResearchService, U.S. Department of Agriculture; or a mixture thereof.

The foregoing has outlined rather broadly the features and technicaladvantages of the present invention in order that the detaileddescription of the invention that follows may be better understood.Additional features and advantages of the invention will be describedhereinafter which form the subject of the claims of the invention. Itshould be appreciated by those skilled in the art that the conceptionand specific embodiment disclosed may be readily utilized as a basis formodifying or designing other structures for carrying out the samepurposes of the present invention. It should also be realized by thoseskilled in the art that such equivalent constructions do not depart fromthe spirit and scope of the invention as set forth in the appendedclaims. The novel features which are believed to be characteristic ofthe invention, both as to its organization and method of operation,together with further objects and advantages will be better understoodfrom the following description when considered in connection with theaccompanying figures. It is to be expressly understood, however, thateach of the figures is provided for the purpose of illustration anddescription only and is not intended as a definition of the limits ofthe present invention.

BRIEF DESCRIPTION OF THE DRAWINGS

For a more complete understanding of the present invention, reference isnow made to the following descriptions taken in conjunction with theaccompanying drawings.

FIGS. 1A and 1B illustrate structures of didemnin B and its exemplarymonomers and other representative didemnins.

FIG. 2 provides detection of didemnin B (upper panel) and nordidemnin B(lower panel) in one of the fractions of Tistrella mobilis crude extractusing UPLC-HRMS analysis.

FIG. 3 shows organization of didemnin NRPS-PKS system.

FIG. 4 illustrates an exemplary incorporation of the two monomers Istand Hip into didemnin B. Reactions are simplified by taking out thethiol-tethered peptidyl intermediates.

FIG. 5 shows an exemplary biosynthetic pathway of didemnin B. C:condensation domain. A: adenylation domain. T: thiolation domain. KR:ketoreductase domain. KS: ketosynthase domain. MT: methyl transferasedomain. TE: thioesterase domain.

FIG. 6 shows detection of didemnin A from Tistrella mobilis.

FIGS. 7A and 7B show detection of didemnin N and nordidemnin AfromTistrella mobilis.

FIGS. 8A and 8B show detection of two new didemnins from Tistrellamobilis.

DETAILED DESCRIPTION OF THE INVENTION

As used herein the specification, “a” or “an” may mean one or more. Asused herein in the claim(s), when used in conjunction with the word“comprising”, the words “a” or “an” may mean one or more than one. Asused herein “another” may mean at least a second or more. In specificembodiments, aspects of the invention may “consist essentially of” or“consist of” one or more sequences of the invention, for example. Someembodiments of the invention may consist of or consist essentially ofone or more elements, method steps, and/or methods of the invention. Itis contemplated that any method or composition described herein can beimplemented with respect to any other method or composition describedherein. Embodiments discussed in the context of methods and/orcompositions of the invention may be employed with respect to any othermethod or composition described herein. Thus, an embodiment pertainingto one method or composition may be applied to other methods andcompositions of the invention as well.

I. DEFINITIONS

The definitions provided in the entire disclosure supersede anyconflicting definition in any of the reference that is incorporated byreference herein. The fact that certain terms are defined, however,should not be considered as indicative that any term that is undefinedis indefinite. Rather, all terms used are believed to describe theinvention in terms such that one of ordinary skill can appreciate thescope and practice the present invention.

The term “didemnin” as used herein refers to a group of cyclicdepsipeptides. In specific embodiments, the didemnin has antitumor,antiviral, and/or immunosuppressive activity.

The term “didemnin derivatives” as used herein refers to any moleculeconstructed based on modification of a didemnin compound. In specificembodiments, the didemnin derivatives have antitumor, antiviral, and/orimmunosuppressive activity.

The term “didemnin gene cluster” as used herein refers to a cluster ofgenes responsible for the biosynthesis of didemnins.

“Effective amount,” “Therapeutically effective amount” or“pharmaceutically effective amount” means that amount which, whenadministered to a subject or patient for treating a disease, issufficient to effect such treatment for the disease, including toimprove at least one symptom of the disease.

The term “growth supporting nutrient medium” is intended to mean anyculture media which include, without limitation, carbon sources,nitrogen sources, amino acids, vitamins and minerals.

As used herein, the term “patient” or “subject” or “individual” refersto a living mammalian organism, such as a human, monkey, cow, sheep,goat, dog, cat, mouse, rat, guinea pig, or transgenic species thereof.In certain embodiments, the patient or subject is a primate.Non-limiting examples of human subjects are adults, juveniles, infantsand fetuses.

“Prevention” or “preventing” includes: (1) inhibiting the onset of adisease in a subject or patient that may be at risk and/or predisposedto the disease but does not yet experience or display any or all of thepathology or symptomatology of the disease, and/or (2) slowing the onsetof the pathology or symptoms of a disease in a subject or patient thatmay be at risk and/or predisposed to the disease but does not yetexperience or display any or all of the pathology or symptoms of thedisease. An individual at risk for cancer, for example, may be anindividual with a family or personal history or that exhibits one ormore known risk factors, such as certain lifestyle habits (for example,smoking) or particular gene-associated mutations (for example, BRCA1 orBRCA2 for breast or ovarian cancer) or particular elevations of ametabolite (elevated prostate-specific antigen (PSA) for prostatecancer).

II. GENERAL EMBODIMENTS OF THE INVENTION

In embodiments of the invention, there are bacteria that producedidemnins or didemnin derivatives, and in particular aspects thebacteria are from the family Rhodospirillales, although in specificaspects the bacteria are from the genus Tistrella.

The present invention at least provides a novel Tistrella mobilisbacterium; amino acid and polynucleotide sequences of the bacterium;didemnins, didemnin precursors, and/or didemnin derivatives producetherefrom; therapeutic compositions containing the didemnins, didemninprecursors, and/or didemnin derivatives; and/or methods for producing orusing the didemnins, didemnin precursors, and/or didemnin derivativecompositions.

III. TISTRELLA MOBILIS SEQUENCES AND THE DIDEMNIN GENE CLUSTER

Embodiments of the invention include isolated polynucleotide andpolypeptide sequences from Tistrella mobilis, such as those included inSEQ ID NOS:10-61. In some cases, the isolated polynucleotide andpolypeptide sequences from Tistrella mobilis are involved in synthesisof one or more didemnins, whereas in other cases the isolatedpolynucleotide and polypeptide sequences from Tistrella mobilis are notinvolved in synthesis of one or more didemnins. The isolatedpolynucleotide or polypeptide sequences may be modified and utilized ina cell or in vitro. In certain cases, the modified polynucleotide orpolypeptide sequences are utilized in a Tistrella mobilis or otherbacterial or yeast cell. Isolated or modified polynucleotide sequencesmay be employed in an expression vector.

Tistrella mobilis gene cluster nucleotide sequences include ORF1 (SEQ IDNO:10), ORF2 (SEQ ID NO:11), ORF3 (SEQ ID NO:12), ORF4 (SEQ ID NO:13),ORF5 (SEQ ID NO:14), ORF6 (SEQ ID NO:15), ORF7 (SEQ ID NO:16), ORF8 (SEQID NO:17), ORF9 (SEQ ID NO:18), ORF10 (SEQ ID NO:19), ORF11 (SEQ IDNO:20), ORF12 (SEQ ID NO:21), ORF13 (SEQ ID NO:22), ORF14 (SEQ IDNO:23), ORF15 (SEQ ID NO:24), and ORF16 (SEQ ID NO:25). CorrespondingTistrella mobilis gene cluster protein sequences for the respective ORFSis as follows: ORF1 (SEQ ID NO:26), ORF2 (SEQ ID NO:27), ORF3 (SEQ IDNO:28), ORF4 (SEQ ID NO:29), ORF5 (SEQ ID NO:30), ORF6 (SEQ ID NO:31),ORF7 (SEQ ID NO:32), ORF8 (SEQ ID NO:33), ORF9 (SEQ ID NO:34), ORF10(SEQ ID NO:35), ORF11 (SEQ ID NO:36), ORF12 (SEQ ID NO:37), ORF13 (SEQID NO:38), ORF14 (SEQ ID NO:39), ORF15 (SEQ ID NO:40), and ORF16 (SEQ IDNO:41).

Tistrella mobilis didemnin gene cluster nucleotide sequences include oneor more of the following: DidA (SEQ ID NO:42); DidB (SEQ ID NO:43), DidC(SEQ ID NO:44), DidD (SEQ ID NO:45), DidE (SEQ ID NO:46), DidF (SEQ IDNO:47), DidG (SEQ ID NO:48), DidH (SEQ ID NO:49), DidI, (SEQ ID NO:50),and DidJ (SEQ ID NO:51). Corresponding Tistrella mobilis didemnin genecluster protein sequences include one or more of the following: DidA(SEQ ID NO:52); DidB (SEQ ID NO:53), DidC (SEQ ID NO:54), DidD (SEQ IDNO:55), DidE (SEQ ID NO:56), DidF (SEQ ID NO:57), DidG (SEQ ID NO:58),DidH (SEQ ID NO:59), DidI, (SEQ ID NO:60), and DidJ (SEQ ID NO:61).

The whole Tistrella mobilis genome is provided by a chromosomal sequence(SEQ ID NO:62) and four mini-chromosomes (which may be referred to asplasmids) including plasmid 1 (SEQ ID NO:63), plasmid 2 (SEQ ID NO:64),plasmid 3 (SEQ ID NO:65), and plasmid 4 (SEQ ID NO:66).

In aspects of the invention, the endogenous Tistrella mobilis didemningene cluster includes polypeptides that are modular according to theparticular domains they employ. They may have one or more of thefollowing domains (one or more) adenylation (A) domain, the thiolation(T) domain, the condensation (C) domain, the ketoreductase (KR) domain,and/or the methyltransferase (MT) domain, for example. For example, didIhas a C domain, an A domain, and a T domain, whereas didB has a C, A, T,and KR domain (see FIG. 3). Marahiel et al. (1997) provide a review ofpeptide synthetases, which is incorporated by reference herein in itsentirety.

IV. DIDEMNINS

Didemnins are cyclic depsipeptide compounds (depsipeptide is a peptidein which one or more of the amide (—CONHR—) bonds are replaced by ester(COOR) bonds). Although more than nine didemnins (didemnins A-E, G, Xand Y) have been isolated from the extract of the exemplary tunicateTrididemnum solidum, in the prior art didemnin B is the one thatpossesses the most potent biological activities. It is a strongantiviral agent against both DNA and RNA viruses such as herpes simplexvirus type 1, a strong immunosuppressant that shows some potential inskin graft and is also very cytotoxic. Although didemnin B shows strongactivity against murine leukemia cells and had completed phase II humanclinical trials against adenocarcinoma of the kidney, advancedepithelial ovarian cancer, and metastatic breast cancer, it exhibitshigh toxicity in human subjects. For review see Vera and Joullié, 2002.

Didemnin precursors, didemnins, and didemnin derivatives are encompassedin methods and compositions of the invention. Exemplary didemnins thatmay be generated in the T. mobilis bacterium of the invention may be ofany kind, but in specific embodiments they are didemnins A-I, M, X andY, nordidemnin B, dehydrodidemnin B (Aplidine®), or are one or more ofthe didemnins described in U.S. Pat. No. 5,294,603, incorporated byreference herein in its entirety. In specific embodiments, didemninderivatives include N-acyl congeners of didemnin A (DA); severalDDB-type analogues of DA in which either pyruvic acid has been replaced(with phenylpyruvic acid or alphaketobutyric acid) or proline atposition 8 has been replaced [with L-azetidine-2-carboxylic acid (AZT),L-pipecolic acid (Pip), 1-amino-1-carboxylic cyclopentane (acc⁵), D-Proor sarcosine (sar); and the didemnins—X[(R)-3-hydroxy-decanoyl-(Gln)³-Lac-Pro didemnin A]; Y[(R)-3-hydroxy-decanoyl-(Gln)₄-Lac-Pro didemnin A]; M(pGlu-Gln-Lac-Pro-didemnin A); N ([Tyr⁵] didemnin B); nordidemnin N([Tyr⁵] nordidemnin B); and epididemnin A ([2S,4R-Hip²] didemnin A).Others include Isodidemnin A₁, Didemnin N, Nordidemnin N, Epididemnin A,Acyclodidemnin A, Dihydrodidemnin N, Dihydroepididemnin A, N-Acetyldidemnin A, N,O-Diacetyldidemnin A, N-(D-Prolyl) didemnin A,N-(benzyloxycarbonyl-D-prolyl) didemnin A, N-(D-Prolyl) didemnin A,N-(L-Prolyl) didemnin A, Acetyl⁹-didemnin B, Propionyl⁹-didemnin B,Isobutyryl⁹-didemnin B, L-Ala⁸-didemnin B, O-Benzyl[L-Ala⁸]didemnin B,L-Ala⁸]didemnin B, [D-Pro⁸]didemnin B, O-Benzyl-[D-pro⁸]didemnin B,[D-Pro^(o)]didemnin B, [N—(CH₃ONsu)didemnin A, O-Acetyldidemnin A,[HexahydroMe₂ Tyr⁵]didemnin A, [Hexahydro-N-MePhe⁵]didemnin A,[HexahydroMe₂ Tyr⁵]didemnin B, [Hexahydro-N-MePhe⁵]didemnin B,pyroglutaminyl didemnin B.

Glutaminyl derivatives as described in U.S. Pat. No. 6,841,530,incorporated by reference herein in its entirety, are also encompassedin the invention. Examples of derivatives include pyroglutaminyldidemnin B, O-Benzyldidemnin B, Benzyloxycarbonyl-L-GlutaminyldidemninB, (Benzyloxycarbonyl-L-Glutaminy)₂ Didemnin, BenzyloxycarbonyldidemninM, Benzyloxycarbony-L-Pyroglutaminyldidemnin B, PyroglutaminyldidemninB, Prolydidemnin A,L-(N-Benzyloxycarbonyl-pyroglutaminyl)-L-glutaminyl-didemnin B,N-Benzyloxycarbonyl-L-pyroglutaminyl-didemnin B, Boc-L-prolyl-didemninA, L-Prolyl-didemnin B (see also US2001/0007855, incorporated byreference herein in its entirety).

In specific aspects, the didemnin is an N-acylated Didemnin A. Inparticular aspects of the invention, the acyl group of the N-acylDidemnin A comprises a C₃ to C₈ group. In certain embodiments, thedidemnin is a derivative of Didemnin A (optionally a syntheticderivative), modified at a position selected from the group consistingof position 1, 5, 6, and combinations thereof, by the incorporation of aD-amino acid (for example). In other aspects, the didemnin is asynthetic derivative of Didemnin A, modified to include aDehydrodidemnin B (DDB) moiety in the linear peptide chain. Inparticular aspects, the didemnin is selected from the group consistingof phenylpyruv-Pro didemnin A, Pyruv-Sar didemnin A,alpha-ketobutyryl-Pro didemnin A, Pyruv-Azt didemnin A, or Pyruv-D-Prodidemnin A.

In some aspects of the invention, one increases the lipophilicity of adidemnin, such as didemnin A, in its modifications, for example to raiseits solubility in the plasma membrane and in at least certain casesincrease its activity. In specific aspects, the N-amine of N-methylD-leucine positions is useful for the addition of hydrophobic groups todidemnin A, for example. From here, one can synthesize a series ofN-acylated analogues of didemnin A comprising alkyl chains with 2, 3, 4,5, 7, 11, 15 or 17 carbon atoms.

Dehydrodidemnin B derivatives which either pyruvic acid has beenreplaced (with phenylpyruvic acid or -ketobutyric acid, for example) orproline at position 8 has been replaced (with L-azetidine-2-carboxylicacid (AZT), L-pipecolic acid (Pip), 1-amino-1-carboxyliccyclopentane(acc₅), D-Pro or sarcosine (sar), for example).

Exemplary hydrophobic derivatives of didemnin A may be synthesized byincorporating acyl chains therein didemnin A, ranging from 4 to 18carbons, for example. Such compounds include N-butyryl didemnin A,N-pentanoyl didemnin A, N-hexanoyl didemnin A, N-octanoyl didemnin A,N-lauroyl didemnin A, N-palmitoyl didemnin A, or N-stearoyl didemnin A.

Three other exemplary derivatives of didemnin A include those in whichthe amino acids at positions 1, 5, and 6 were replaced with theircorresponding D-amino acids. Exemplary compounds include D-Thr¹ didemninA, D-Pro⁵] didemnin A, and D-MeTyr(Me)⁶] didemnin A.

Five didemnin A derivatives related to dehydrodidemnin B may besynthesized by introducing DDB-type modifications into their linearpeptide chain moieties. Such DDB-type compounds include Phenylpyruv-Prodidemnin A, Pyruv-Sar didemnin A-ketobutyryl-Pro didemnin A, Pyruv-Aztdidemnin A, and Pyruv-D-Po didemnin A.

Synthesis of N-acyl analogues may be carried out in solution. Acylgroups may be introduced at the N-methyl-D-leucine unit of didemnin Ausing a symmetrical anhydride procedure with C₆ to C₁₈ fatty acids, andan excess of symmetrical anhydride may be required in order to achievecomplete acylation. Propionic butyric and pentanoic acids may beintroduced using a 3-5 fold excess of their symmetrical anhydride.

The symmetrical anhydrides of fatty acids may be prepared in theconventional manner using EDC. Acylation of didemnin A may be carriedout in the presence of a catalytic amount of dimethylamino pyridine(DMAP). Derivatives may be purified on a silica gel column usingmethanol/chloroform as a eluant. They and others may be characterizedusing ¹H NMR spectroscopy and HRFABMS. Exemplary methods of synthesis ofdidemnin analogs is described in U.S. Pat. No. 5,294,603, which isincorporated by reference herein in its entirety.

Structure elucidation, chemical conversion, biological activitiesincluding cytotoxicity, antiviral and immunosuppressive activities andstructure-activity relationships may be performed for any didemnin ordidemnin derivative of the invention.

V. PRODUCTION OF MODIFIED ORGANISMS FOR DIDEMNIN PRECURSORS, DIDEMNINS,AND/OR DIDEMNIN DERIVATIVES, AND COMPOSITIONS PRODUCED THEREBY

In some embodiments of the invention, one or more host cells (which maybe organisms) are modified to produce one or more didemnin precursors,didemnins, or didemnin derivatives. In some cases the organism is fromthe Tistrella genus, such as Tistrella mobilis, for example, although inother cases an organism or other cells are modified to produce didemninprecursors, didemnins, or didemnin derivatives.

A. Tistrella

In certain embodiments of the invention, a Tistrella bacterium ismodified to affect the synthesis of one or more endogenous didemninsproduced in the native bacterium. In some cases, the modified bacteriumis genetically modified (such as by using recombinant technology tomutate or knock out one or more endogenous bacteria genes, including bytransforming the bacteria with a polynucleotide construct, for example),although it may also or otherwise be chemically modified (daunorubicinor nitrosoguanidine, for example) and/or physically modified (gamma orultraviolet radiation, for example).

For genetic modification of Tistrella (or other organisms), nucleic acidmolecules comprising a mutation of interest or means to generate amutation of interest) of the present invention can be expressedseparately, i.e., inserted into separate vectors for expression. Suchvectors are known or can be constructed by those skilled in the art andgenerally contain all expression elements (e.g., promoters, terminatorfragments, enhancer elements, marker genes and other elements asappropriate) necessary to achieve the desired transcription of thesequences. Examples of vectors include viruses such as bacteriophages,baculoviruses, and retroviruses, DNA viruses, cosmids, plasmids,phagemids and other recombination vectors. The vectors can also containelements for use in either prokaryotic or eukaryotic host systems. Oneof ordinary skill in the art will know which host systems are compatiblewith a particular vector. The vectors can be introduced into cells ortissues and expressed by any one of a variety of known methods withinthe art. Such methods can be found generally described in Sambrook etal. (1989, 1992) Molecular Cloning: A Laboratory Manual, Cold SpringsHarbor Laboratory, New York; Ausubel et al. (1989) Current Protocols inMolecular Biology, John Wiley and Sons, Baltimore, Md.; Chang, et al.(1995) Somatic Gene Therapy, CRC Press, Ann Arbor, Mich.; Vega, et al.(1995) Gene Targeting, CRC Press, Ann Arbor, Mich.; Vectors: A Survey ofMolecular Cloning Vectors and Their Uses, Butterworths, Boston, Mass.(1988); and include, for example, stable or transient transfection,lipofection, electroporation and infection with recombinant viralvectors. Introduction of nucleic acids by infection offers severaladvantages over other listed methods. Higher efficiencies can beobtained due to their infectious nature. Moreover, viruses are veryspecialized and typically infect and propagate in specific cell types.Thus, their natural specificity can be used to target the vectors tospecific cell types in vivo or within a tissue or mixed culture ofcells. The viral vectors can also be modified with specific receptors orligands to alter target specificity through receptor mediated events.

B. Non-Tistrella Cells

Host cells suitable for introduction and expression of the nucleic acidsof the invention may be bacterial; however, yeast (e.g., Pichia,Saccharomyces, etc.), mammalian, or insect host cells are alsocontemplated, as is a cell-free expression system. In particularembodiments, the host cell or culture is bacterial. Exemplary bacterialhost cells include E. coli as well as Bacillus sp.

In cases wherein Tistrella is not used as a fermentation or other systemfor production of modified didemnin and didemnin-related compounds, thehost cell may be modified to include one or more proteins suitable forproduction of at least one didemnin precursor, didemnin, and/or didemninderivative. In particular, the host cell may be modified to include partor all of a didemnin gene cluster, including one or more of didA-didJand/or ORF1-16, for example. In specific embodiments, the cell includesORF1, ORF3, ORF6, ORF 7, ORF 8 and/or ORF 16. In particular aspects, oneor more of ORF1, ORF3, ORF6, ORF 7, ORF 8 and/or ORF 16 are involved inthe regulation, transport, resistance and other functions of thedidemnin gene cluster. In some cases, the host cell may be modified toinclude a mutated did gene (compared to Tistrella wildtype sequence)such that a didemnin derivative may be produced, and it optionally mayalso include one or more of the endogenous Tistrella wild-type didgenes. The didemnin derivative may be produced directly therefrom themodified host cell, or a didemnin precursor may be produced andadditional synthesis steps may be required following purification of theprecursor, for example.

C. Exemplary Modifications of Host Cells

In particular aspects of the invention, a host cell (Tistrella or E.coli, for example) is modified compared to their respective wild-typecounterparts. The modification may be such that one or more endogenouspolynucleotides in the host cell become mutated compared to theirrespective wild-type counterparts. The mutation may be of any kind,including a knockout, knockdown, point, frameshift, inversion, deletion,and so forth. The mutation may be of any kind so as to effect an alteredsynthesis of one or more of a didemnin precursor, didemnin, or didemninderivative.

In some cases, one or more mutations are generated in one or moredidemnin gene cluster members, including one or more of didA-didJ,and/or in one or more of ORF1-ORF16. In certain cases, a particularregion of one or more didemnin gene cluster members is mutated. Theregion may be of any kind so as to effect an altered synthesis of one ormore of a didemnin precursor, didemnin, or didemnin derivative. Theaffected region may be in the sequence that encodes (one or more)adenylation (A) domain, the thiolation (T) domain, the condensation (C)domain, the ketoreductase (KR) domain, and/or the methyltransferase (MT)domain, for example.

In particular aspects the A domain is modified, for example such thatthe A domain specificity and/or placement within the non-ribosomalpeptide synthetase assembly affects the sequence of the monomers in thenonribosomal peptide. In specific cases, the particular DidA-DidJ genesis modified such that the substrate specificity is different. Forexample, the DidA A domain may be altered to have substrate specificityother than glutamine, DidC may be altered to have substrate specificityother than proline, and so forth (see Table 3 of predicted normalsubstrate specificities).

In particular cases, one can genetically modify one or more genes of thedidemnin gene cluster such that the pathway generates a didemninderivative or didemnin precursor that can then be modified followingpurification of the precursor. For example, one can knock out one ormore genes or one or more regions of one or more genes. For example, onecan knock out the ketoreductase in DidB so that the pathway producesdehydrodidemnin B. In other exemplary measures, one can replace one ofthe A domains with another A domain in any of the members of the genecluster, for example. For example, one can replace one of the A domainswith another A domain (for example, one that activate Valine) such thata different didemnin with a Valine residue will be produced.

In some cases, the mutation is generated in the host cell. Followingthis, the mutant strain can be cultured to produce the desired didemninprecursor, didemnin, or didemnin derivative, and the compound can thenbe purified (for example, by one or more of extraction, fractionation bynormal or reverse phase liquid chromatography, size-exclusionchromatography, reverse-phase HPLC, and so forth). If appropriate,further modifications to the compound may be produced. In some cases,one can culture the mutant strain and wild-type strain and extract themrespectively; then, one can compare their respective metabolites, forexample by LC-HRMS (high resolution mass spectrometry) and/or NMR toidentify or verify production of the expected metabolite.

In alternative embodiments, one can mutagenize a plurality of host cells(including Tistrella or E. coli) for example and performs a highthroughput assay to obtain the mutant. The candidate mutants producingdidemnin precursors, didemnins, or didemnin derivatives may be screenedfor a compound not otherwise obtained from the corresponding wild-typehost cell or obtainable to much higher levels than in the correspondingwildtype host cell.

VI. DETERMINATION OF DIDEMNIN OR DIDEMNIN DERIVATIVE STRUCTURE

In embodiments of the invention, the didemnin or didemnin derivative isisolated and the structure is determined. Although one of skill in theart recognizes routine methods of purifying and determining a chemicalstructure, in specific cases one may use extraction (including, forexample, methanol-toluene (or ethyl acetate or chloroform) extraction),silica gel, preparative thin-layer chromatrography, nuclear magneticresonance imaging, acid hydrolysis, mass spectrometry, gaschromatography, x-ray crystallography, and a combination thereof.

VII. EXEMPLARY SYNTHESIS EMBODIMENTS

In certain cases, a didemnin precursor, didemnin, and/or didemninderivative compounds is obtained by bacteria or other fermentationmethods of the invention and is then processed. The processing mayinclude further purification, although in some embodiments the compoundis subject to one or more further synthesis steps to obtain the desiredmolecule. Any suitable further synthesis steps may be employed in theart and are known to the skilled artisan (for representative examples,see Mayer et al., (1994); Jou et al. (1997)).

In specific aspects of the invention, there is in vitro conversion ofdidemnin B from T. mobilis to dehydrodidemnin B, for example using anoxidizing agent to oxidize the didemnin B to dehydrodidemnin B (seeFaulkner D J. Marine pharmacology. (2000) Antonie van Leeuwenhoek 77:135-145, incorporated by reference herein in its entirety).

Thus, compounds of the present disclosure may be made using the methodsknown in the art. These methods can be further modified and optimizedusing the principles and techniques of organic chemistry as applied by aperson skilled in the art. Such principles and techniques are taught,for example, in March's Advanced Organic Chemistry: Reactions,Mechanisms, and Structure (2007), which is incorporated by referenceherein in its entirety.

Compounds encompassed in methods of the invention or produced directlyor indirectly there by may contain one or moreasymmetrically-substituted carbon or nitrogen atoms, and may be isolatedin optically active or racemic form. Thus, all chiral, diastereomeric,racemic form, epimeric form, and all geometric isomeric forms of astructure are intended, unless the specific stereochemistry or isomericform is specifically indicated. Compounds may occur as racemates andracemic mixtures, single enantiomers, diastereomeric mixtures andindividual diastereomers. In some embodiments, a single diastereomer isobtained. The chiral centers of the compounds of the present inventioncan have the S or the R configuration.

Compounds of the invention may also have the advantage that they may bemore efficacious than, be less toxic than, be longer acting than, bemore potent than, produce fewer side effects than, be more easilyabsorbed than, and/or have a better pharmacokinetic profile (e.g.,higher oral bioavailability and/or lower clearance) than, and/or haveother useful pharmacological, physical, or chemical properties over,compounds known in the prior art, whether for use in the indicationsstated herein or otherwise.

In addition, atoms making up the compounds of the present invention areintended to include all isotopic forms of such atoms. Isotopes, as usedherein, include those atoms having the same atomic number but differentmass numbers. By way of general example and without limitation, isotopesof hydrogen include tritium and deuterium, and isotopes of carboninclude ¹³C and ¹⁴C.

It should be recognized that the particular anion or cation forming apart of any salt of this invention is not critical, so long as the salt,as a whole, is pharmacologically acceptable. Additional examples ofpharmaceutically acceptable salts and their methods of preparation anduse are presented in Handbook of Pharmaceutical Salts: Properties, andUse (2002), which is incorporated herein by reference.

When used in the context of a chemical group, “hydrogen” means —H;“hydroxy” means —OH; “oxo” means ═O; “halo” means independently —F, —Cl,—Br or —I; “amino” means —NH₂ (see below for definitions of groupscontaining the term amino, e.g., alkylamino); “hydroxyamino” means—NHOH; “nitro” means —NO₂; imino means ═NH (see below for definitions ofgroups containing the term imino, e.g., alkylimino); “cyano” means —CN;“isocyanate” means —N═C═O; “azido” means —N₃; in a monovalent context“phosphate” means —OP(O)(OH)₂ or a deprotonated form thereof; in adivalent context “phosphate” means —OP(O)(OH)O— or a deprotonated formthereof; “mercapto” means —SH; “thio” means ═S; “thioether” means —S—;“sulfonamido” means —NHS(O)₂— (see below for definitions of groupscontaining the term sulfonamido, e.g., alkylsulfonamido); “sulfonyl”means —S(O)₂— (see below for definitions of groups containing the termsulfonyl, e.g., alkylsulfonyl); and “sulfinyl” means —S(O)— (see belowfor definitions of groups containing the term sulfinyl, e.g.,alkylsulfinyl).

In the context of chemical formulas, the symbol “—” means a single bond,“═” means a double bond, and “≡” means triple bond. The symbol “_(————)”represents an optional bond, which if present is either single ordouble. The symbol “

” represents a single bond or a double bond. Thus, for example, thestructure

includes the structures

As will be understood by a person of skill in the art, no one such ringatom forms part of more than one double bond. The symbol “

”, when drawn perpendicularly across a bond indicates a point ofattachment of the group. It is noted that the point of attachment istypically only identified in this manner for larger groups in order toassist the reader in rapidly and unambiguously identifying a point ofattachment. The symbol “

” means a single bond where the group attached to the thick end of thewedge is “out of the page.” The symbol “

” means a single bond where the group attached to the thick end of thewedge is “into the page”. The symbol “

” means a single bond where the conformation (e.g., either R or S) orthe geometry is undefined (e.g., either E or Z).

Any undefined valency on an atom of a structure shown in thisapplication implicitly represents a hydrogen atom bonded to the atom.When a group “R” is depicted as a “floating group” on a ring system, forexample, in the formula:

then R may replace any hydrogen atom attached to any of the ring atoms,including a depicted, implied, or expressly defined hydrogen, so long asa stable structure is formed. When a group “R” is depicted as a“floating group” on a fused ring system, as for example in the formula:

then R may replace any hydrogen attached to any of the ring atoms ofeither of the fused rings unless specified otherwise. Replaceablehydrogens include depicted hydrogens (e.g., the hydrogen attached to thenitrogen in the formula above), implied hydrogens (e.g., a hydrogen ofthe formula above that is not shown but understood to be present),expressly defined hydrogens, and optional hydrogens whose presencedepends on the identity of a ring atom (e.g., a hydrogen attached togroup X, when X equals —CH—), so long as a stable structure is formed.In the example depicted, R may reside on either the 5-membered or the6-membered ring of the fused ring system. In the formula above, thesubscript letter “y” immediately following the group “R” enclosed inparentheses, represents a numeric variable. Unless specified otherwise,this variable can be 0, 1, 2, or any integer greater than 2, onlylimited by the maximum number of replaceable hydrogen atoms of the ringor ring system.

For the groups and classes below, the following parenthetical subscriptsfurther define the group/class as follows: “(Cn)” defines the exactnumber (n) of carbon atoms in the group/class. “(C≦n)” defines themaximum number (n) of carbon atoms that can be in the group/class, withthe minimum number as small as possible for the group in question, e.g.,it is understood that the minimum number of carbon atoms in the group“alkenyl_((C≦8))” or the class “alkene_((C≦8))” is two. For example,“alkoxy_((C≦10))” designates those alkoxy groups having from 1 to 10carbon atoms (e.g., 1, 2, 3, 4, 5, 6, 7, 8, 9, or 10, or any rangederivable therein (e.g., 3 to 10 carbon atoms). (Cn-n′) defines both theminimum (n) and maximum number (n′) of carbon atoms in the group.Similarly, “alkyl_((C2-10))” designates those alkyl groups having from 2to 10 carbon atoms (e.g., 2, 3, 4, 5, 6, 7, 8, 9, or 10, or any rangederivable therein (e.g., 3 to 10 carbon atoms)).

The term “saturated” as used herein means the compound or group somodified has no carbon-carbon double and no carbon-carbon triple bonds,except as noted below. The term does not preclude carbon-heteroatommultiple bonds, for example a carbon oxygen double bond or a carbonnitrogen double bond. Moreover, it does not preclude a carbon-carbondouble bond that may occur as part of keto-enol tautomerism orimine/enamine tautomerism.

The term “aliphatic” when used without the “substituted” modifiersignifies that the compound/group so modified is an acyclic or cyclic,but non-aromatic hydrocarbon compound or group. In aliphaticcompounds/groups, the carbon atoms can be joined together in straightchains, branched chains, or non-aromatic rings (alicyclic). Aliphaticcompounds/groups can be saturated, that is joined by single bonds(alkanes/alkyl), or unsaturated, with one or more double bonds(alkenes/alkenyl) or with one or more triple bonds (alkynes/alkynyl).When the term “aliphatic” is used without the “substituted” modifieronly carbon and hydrogen atoms are present. When the term is used withthe “substituted” modifier one or more hydrogen atom has beenindependently replaced by one of the following exemplary non-limitingfunctional groups: —OH, —F, —Cl, —Br, —I, —NH₂, —NO₂, —CO₂H, —CO₂CH₃,—CN, —SH, —OCH₃, —OCH₂CH₃, —C(O)CH₃, —N(CH₃)₂, —C(O)NH₂, —B(OH)₂,—P(O)(OCH₃)₂ or —OC(O)CH₃.

The term “alkyl” when used without the “substituted” modifier refers toa monovalent saturated aliphatic group with a carbon atom as the pointof attachment, a linear or branched, cyclo, cyclic or acyclic structure,and no atoms other than carbon and hydrogen. Thus, as used hereincycloalkyl is a subset of alkyl. The groups —CH₃ (Me), —CH₂CH₃ (Et),—CH₂CH₂CH₃ (n-Pr), —CH(CH₃)₂ (iso-Pr), —CH(CH₂)₂ (cyclopropyl),—CH₂CH₂CH₂CH₃ (n-Bu), —CH(CH₃)CH₂CH₃ (sec-butyl), —CH₂CH(CH₃)₂(iso-butyl), —C(CH₃)₃ (tert-butyl), —CH₂C(CH₃)₃ (neo-pentyl),cyclobutyl, cyclopentyl, cyclohexyl, and cyclohexylmethyl arenon-limiting examples of alkyl groups. The term “alkanediyl” when usedwithout the “substituted” modifier refers to a divalent saturatedaliphatic group, with one or two saturated carbon atom(s) as thepoint(s) of attachment, a linear or branched, cyclo, cyclic or acyclicstructure, no carbon-carbon double or triple bonds, and no atoms otherthan carbon and hydrogen. The groups, —CH₂— (methylene), —CH₂CH₂—,—CH₂C(CH₃)₂CH₂—, —CH₂CH₂CH₂—, and

are non-limiting examples of alkanediyl groups. The term “alkylidene”when used without the “substituted” modifier refers to the divalentgroup ═CRR′ in which R and R′ are independently hydrogen, alkyl, or Rand R′ are taken together to represent an alkanediyl having at least twocarbon atoms. Non-limiting examples of alkylidene groups include: ═CH₂,═CH(CH₂CH₃), and ═C(CH₃)₂. When the term is used with the “substituted”modifier one or more hydrogen atom has been independently replaced byone of the following exemplary non-limiting functional groups: —OH, —F,—Cl, —Br, —I, —NH₂, —NO₂, —CO₂H, —CO₂CH₃, —CN, —SH, —OCH₃, —OCH₂CH₃,—C(O)CH₃, —N(CH₃)₂, —C(O)NH₂, —B(OH)₂, —P(O)(OCH₃)₂ or —OC(O)CH₃. Thefollowing groups are non-limiting examples of substituted alkyl groups:—CH₂OH, —CH₂Cl, —CF₃, —CH₂CN, —CH₂C(O)OH, —CH₂C(O)OCH₃, —CH₂C(O)NH₂,—CH₂C(O)CH₃, —CH₂OCH₃, —CH₂OC(O)CH₃, —CH₂NH₂, —CH₂N(CH₃)₂, and—CH₂CH₂Cl. The term “fluoroalkyl” is a subset of substituted alkyl, inwhich one or more hydrogen has been substituted with a fluoro group andno other atoms aside from carbon, hydrogen and fluorine are present. Thegroups, —CH₂F, —CF₃, and —CH₂CF₃ are non-limiting examples offluoroalkyl groups. An “alkane” refers to the compound H—R, wherein R isalkyl.

The term “aryl” when used without the “substituted” modifier refers to amonovalent unsaturated aromatic group with an aromatic carbon atom asthe point of attachment, said carbon atom forming part of a one or moresix-membered aromatic ring structure, wherein the ring atoms are allcarbon, and wherein the group consists of no atoms other than carbon andhydrogen. If more than one ring is present, the rings may be fused orunfused. As used herein, the term does not preclude the presence of oneor more alkyl group (carbon number limitation permitting) attached tothe first aromatic ring or any additional aromatic ring present.Non-limiting examples of aryl groups include phenyl (Ph), methylphenyl,(dimethyl)phenyl, —C₆H₄—CH₂CH₃ (ethylphenyl), naphthyl, and themonovalent group derived from biphenyl. The term “arenediyl” when usedwithout the “substituted” modifier refers to a divalent aromatic group,with two aromatic carbon atoms as points of attachment, said carbonatoms forming part of one or more six-membered aromatic ringstructure(s) wherein the ring atoms are all carbon, and wherein themonovalent group consists of no atoms other than carbon and hydrogen. Asused herein, the term does not preclude the presence of one or morealkyl group (carbon number limitation permitting) attached to the firstaromatic ring or any additional aromatic ring present. If more than onering is present, the rings may be fused or unfused. Non-limitingexamples of arenediyl groups include:

When the term “aryl” is used with the “substituted” modifier one or morehydrogen atom has been independently replaced by one of the followingexemplary non-limiting functional groups: —OH, —F, —Cl, —Br, —I, —NH₂,—NO₂, —CO₂H, —CO₂CH₃, —CN, —SH, —OCH₃, —OCH₂CH₃, —C(O)CH₃, —N(CH₃)₂,—C(O)NH₂, —B(OH)₂, —P(O)(OCH₃)₂ or —OC(O)CH₃. An “arene” refers to thecompound H—R, wherein R is aryl.

The term “aralkyl” when used without the “substituted” modifier refersto the monovalent group -alkanediyl-aryl, in which the terms alkanediyland aryl are each used in a manner consistent with the definitionsprovided above. Non-limiting examples of aralkyls are: phenylmethyl(benzyl, Bn) and 2-phenyl-ethyl. When the term is used with the“substituted” modifier one or more hydrogen atom has been independentlyreplaced by one of the following exemplary non-limiting functionalgroups: —OH, —F, —Cl, —Br, —I, —NH₂, —NO₂, —CO₂H, —CO₂CH₃, —CN, —SH,—OCH₃, —OCH₂CH₃, —C(O)CH₃, —N(CH₃)₂, —C(O)NH₂, —B(OH)₂, —P(O)(OCH₃)₂ or—OC(O)CH₃. Non-limiting examples of substituted aralkyls are:(3-chlorophenyl)-methyl, and 2-chloro-2-phenyl-eth-1-yl.

The term “heteroaryl” when used without the “substituted” modifierrefers to a monovalent aromatic group with an aromatic carbon atom ornitrogen atom as the point of attachment, said carbon atom or nitrogenatom forming part of an aromatic ring structure wherein at least one ofthe ring atoms is nitrogen, oxygen or sulfur, and wherein the groupconsists of no atoms other than carbon, hydrogen, aromatic nitrogen,aromatic oxygen and aromatic sulfur. As used herein, the term does notpreclude the presence of one or more alkyl group (carbon numberlimitation permitting) attached to the aromatic ring or any additionalaromatic ring present. Non-limiting examples of heteroaryl groupsinclude furanyl, imidazolyl, indolyl, indazolyl (Im), methylpyridyl,oxazolyl, pyridyl, pyrrolyl, pyrimidyl, pyrazinyl, quinolyl, quinazolyl,quinoxalinyl, thienyl, and triazinyl. The term “heteroarenediyl” whenused without the “substituted” modifier refers to an divalent aromaticgroup, with two aromatic carbon atoms, two aromatic nitrogen atoms, orone aromatic carbon atom and one aromatic nitrogen atom as the twopoints of attachment, said atoms forming part of one or more aromaticring structure(s) wherein at least one of the ring atoms is nitrogen,oxygen or sulfur, and wherein the divalent group consists of no atomsother than carbon, hydrogen, aromatic nitrogen, aromatic oxygen andaromatic sulfur. As used herein, the term does not preclude the presenceof one or more alkyl group (carbon number limitation permitting)attached to the first aromatic ring or any additional aromatic ringpresent. If more than one ring is present, the rings may be fused orunfused. Non-limiting examples of heteroarenediyl groups include:

When the term is used with the “substituted” modifier one or morehydrogen atom has been independently replaced by one of the followingexemplary non-limiting functional groups: —OH, —F, —Cl, —Br, —I, —NH₂,—NO₂, —CO₂H, —CO₂CH₃, —CN, —SH, —OCH₃, —OCH₂CH₃, —C(O)CH₃, —N(CH₃)₂,—C(O)NH₂, —B(OH)₂, —P(O)(OCH₃)₂ or —OC(O)CH₃.

The term “acyl” when used without the “substituted” modifier refers tothe group —C(O)R, in which R is a hydrogen, alkyl, aryl, aralkyl orheteroaryl, as those terms are defined above. The groups, —CHO, —C(O)CH₃(acetyl, Ac), —C(O)CH₂CH₃, —C(O)CH₂CH₂CH₃, —C(O)CH(CH₃)₂, —C(O)CH(CH₂)₂,—C(O)C₆H₅, —C(O)C₆H₄—CH₃, —C(O)CH₂C₆H₅, —C(O)(imidazolyl) arenon-limiting examples of acyl groups. A “thioacyl” is defined in ananalogous manner, except that the oxygen atom of the group —C(O)R hasbeen replaced with a sulfur atom, —C(S)R. When the term is used with the“substituted” modifier one or more hydrogen atom has been independentlyreplaced by one of the following exemplary non-limiting functionalgroups: —OH, —F, —Cl, —Br, —I, —NH₂, —NO₂, —CO₂H, —CO₂CH₃, —CN, —SH,—OCH₃, —OCH₂CH₃, —C(O)CH₃, —N(CH₃)₂, —C(O)NH₂, —B(OH)₂, —P(O)(OCH₃)₂ or—OC(O)CH₃. The groups, —C(O)CH₂CF₃, —CO₂H (carboxyl), —CO₂CH₃(methylcarboxyl), —CO₂CH₂CH₃, —C(O)NH₂ (carbamoyl), and —CON(CH₃)₂, arenon-limiting examples of substituted acyl groups.

The term “alkoxy” when used without the “substituted” modifier refers tothe group —OR, in which R is an alkyl, as that term is defined above.Non-limiting examples of alkoxy groups include: —OCH₃, —OCH₂CH₃,—OCH₂CH₂CH₃, —OCH(CH₃)₂, —OCH(CH₂)₂, —O-cyclopentyl, and —O-cyclohexyl.The terms “alkenyloxy”, “alkynyloxy”, “aryloxy”, “aralkoxy”,“heteroaryloxy”, and “acyloxy”, when used without the “substituted”modifier, refers to groups, defined as —OR, in which R is alkenyl,alkynyl, aryl, aralkyl, heteroaryl, and acyl, respectively. Similarly,the term “alkylthio” when used without the “substituted” modifier refersto the group —SR, in which R is an alkyl, as that term is defined above.When the term is used with the “substituted” modifier one or morehydrogen atom has been independently replaced by one of the followingexemplary non-limiting functional groups: —OH, —F, —Cl, —Br, —I, —NH₂,—NO₂, —CO₂H, —CO₂CH₃, —CN, —SH, —OCH₃, —OCH₂CH₃, —C(O)CH₃, —N(CH₃)₂,—C(O)NH₂, —B(OH)₂, —P(O)(OCH₃)₂ or —OC(O)CH₃. The term “alcohol”corresponds to an alkane, as defined above, wherein at least one of thehydrogen atoms has been replaced with a hydroxy group.

The term “alkylamino” when used without the “substituted” modifierrefers to the group —NHR, in which R is an alkyl, as that term isdefined above. Non-limiting examples of alkylamino groups include:—NHCH₃ and —NHCH₂CH₃. The term “dialkylamino” when used without the“substituted” modifier refers to the group —NRR′, in which R and R′ canbe the same or different alkyl groups, or R and R′ can be taken togetherto represent an alkanediyl. Non-limiting examples of dialkylamino groupsinclude: —N(CH₃)₂, —N(CH₃)(CH₂CH₃), and N-pyrrolidinyl. The terms“alkoxyamino”, “alkenylamino”, “alkynylamino”, “arylamino”,“aralkylamino”, “heteroarylamino”, and “alkylsulfonylamino” when usedwithout the “substituted” modifier, refers to groups, defined as —NHR,in which R is alkoxy, alkenyl, alkynyl, aryl, aralkyl, heteroaryl, andalkylsulfonyl, respectively. A non-limiting example of an arylaminogroup is —NHC₆H₅. The term “amido” (acylamino), when used without the“substituted” modifier, refers to the group —NHR, in which R is acyl, asthat term is defined above. A non-limiting example of an amido group is—NHC(O)CH₃. The term “alkylimino” when used without the “substituted”modifier refers to the divalent group ═NR, in which R is an alkyl, asthat term is defined above. When the term is used with the “substituted”modifier one or more hydrogen atom has been independently replaced byone of the following exemplary non-limiting functional groups: —OH, —F,—Cl, —Br, —I, —NH₂, —NO₂, —CO₂H, —CO₂CH₃, —CN, —SH, —OCH₃, —OCH₂CH₃,—C(O)CH₃, —N(CH₃)₂, —C(O)NH₂, —B(OH)₂, —P(O)(OCH₃)₂ or —OC(O)CH₃. Thegroups —NHC(O)OCH₃ and —NHC(O)NHCH₃ are non-limiting examples ofsubstituted amido groups.

The term “heterocyclic” or “heterocycle” when used without the“substituted” modifier signifies that the compound/group so modifiedcomprising at least one ring in which at least one ring atom is anelement other than carbon. Examples of the non-carbon ring atoms includebut are not limited to nitrogen, oxygen, sulfur, boron, phosphorus,arsenic, antimony, germanium, bismuth, silicon and/or tin. Examples ofheterocyclic structures include but are not limited to aziridine,azirine, oxirane, epoxide, oxirene, thiirane, episulfides, thiirene,diazirine, oxaziridine, dioxirane, azetidine, azete, oxetane, oxete,thietane, thiete, diazetidine, dioxetane, dioxete, dithietane, dithiete,pyrrolidine, pyrrole, oxolane, furane, thiolane, thiophene, borolane,borole, phospholane, phosphole, arsolane, arsole, stibolane, stibole,bismolane, bismole, silolane, silole, stannolane, stannole,imidazolidine, imidazole, pyrazolidine, pyrazole, imidazoline,pyrazoline, oxazolidine, oxazole, oxazoline, isoxazolidine, isoxazole,thiazolidine, thiazole, thiazoline, isothiazolidine, isothiazole,dioxolane, thithiolane, triazole, furazan, oxadiazole, thiadiazole,dithiazole, tetrazole, piperidine, pyridine, oxane, pyran, thiane,thiopyran, salinane, saline, germinane, germine, stanninane, stannine,borinane, borinine, phosphinane, phosphinine, arsinane, arsinine,piperazine, diazine, morpholine, oxazine, thiomorpholine, thiazine,dioxane, dioxine, dithiane, dithiine, triazine, trioxane, tetrazine,azepane, azepine, oxepane, oxepine, thiepane, thiepine, homopiperazine,diazepine, thiazepine, ozocane, azocine, oxecane, or thiocane. When theterm “heterocyclic” is used with the “substituted” modifier one or morehydrogen atom has been independently replaced by one of the followingexemplary non-limiting functional groups: —OH, —F, —Cl, —Br, —I, —NH₂,—NO₂, —CO₂H, —CO₂CH₃, —CN, —SH, —OCH₃, —OCH₂CH₃, —C(O)CH₃, —N(CH₃)₂,—C(O)NH₂ or —OC(O)CH₃.

As generally used herein “pharmaceutically acceptable” refers to thosecompounds, materials, compositions, and/or dosage forms which are,within the scope of sound medical judgment, suitable for use in contactwith the tissues, organs, and/or bodily fluids of human beings andanimals without excessive toxicity, irritation, allergic response, orother problems or complications commensurate with a reasonablebenefit/risk ratio.

“Pharmaceutically acceptable salts” means salts of compounds of thepresent invention which are pharmaceutically acceptable, as definedabove, and which possess the desired pharmacological activity. Suchsalts include acid addition salts formed with inorganic acids such ashydrochloric acid, hydrobromic acid, sulfuric acid, nitric acid,phosphoric acid, and the like; or with organic acids such as1,2-ethanedisulfonic acid, 2-hydroxyethanesulfonic acid,2-naphthalenesulfonic acid, 3-phenylpropionic acid,4,4′-methylenebis(3-hydroxy-2-ene-1-carboxylic acid),4-methylbicyclo[2.2.2]oct-2-ene-1-carboxylic acid, acetic acid,aliphatic mono- and dicarboxylic acids, aliphatic sulfuric acids,aromatic sulfuric acids, benzenesulfonic acid, benzoic acid,camphorsulfonic acid, carbonic acid, cinnamic acid, citric acid,cyclopentanepropionic acid, ethanesulfonic acid, fumaric acid,glucoheptonic acid, gluconic acid, glutamic acid, glycolic acid,heptanoic acid, hexanoic acid, hydroxynaphthoic acid, lactic acid,laurylsulfuric acid, maleic acid, malic acid, malonic acid, mandelicacid, methanesulfonic acid, muconic acid, o-(4-hydroxybenzoyl)benzoicacid, oxalic acid, p-chlorobenzenesulfonic acid, phenyl-substitutedalkanoic acids, propionic acid, p-toluenesulfonic acid, pyruvic acid,salicylic acid, stearic acid, succinic acid, tartaric acid,tertiarybutylacetic acid, trimethylacetic acid, trifluoroacetic acid,trifluormethyl sulfonic (triflic) acid and the like. Pharmaceuticallyacceptable salts also include base addition salts which may be formedwhen acidic protons present are capable of reacting with inorganic ororganic bases. Acceptable inorganic bases include sodium hydroxide,sodium carbonate, potassium hydroxide, aluminum hydroxide and calciumhydroxide. Acceptable organic bases include, but are not limited toethanolamine, diethanolamine, triethanolamine, tromethamine,N-methylglucamine and the like. It should be recognized that theparticular anion or cation forming a part of any salt of this inventionis not critical, so long as the salt, as a whole, is pharmacologicallyacceptable. Additional examples of pharmaceutically acceptable salts andtheir methods of preparation and use are presented in Handbook ofPharmaceutical Salts: Properties, and Use (P. H. Stahl & C. G. Wermutheds., Verlag Helvetica Chimica Acta, 2002).

VIII. PHARMACEUTICAL PREPARATIONS

Pharmaceutical compositions of the present invention comprise aneffective amount of one or more didemnin or didemnin derivativecompositions of the invention dissolved or dispersed in apharmaceutically acceptable carrier. The phrases “pharmaceutical orpharmacologically acceptable” refers to molecular entities andcompositions that do not produce an adverse, allergic or other untowardreaction when administered to an animal, such as, for example, a human,as appropriate. The preparation of an pharmaceutical composition thatcontains at least one composition of the invention or additional activeingredient will be known to those of skill in the art in light of thepresent disclosure, as exemplified by Remington's PharmaceuticalSciences, 18th Ed. Mack Printing Company, 1990, incorporated herein byreference. Moreover, for animal (e.g., human) administration, it will beunderstood that preparations should meet sterility, pyrogenicity,general safety and purity standards as required by FDA Office ofBiological Standards.

As used herein, “pharmaceutically acceptable carrier” includes any andall solvents, dispersion media, coatings, surfactants, antioxidants,preservatives (e.g., antibacterial agents, antifungal agents), isotonicagents, absorption delaying agents, salts, preservatives, drugs, drugstabilizers, gels, binders, excipients, disintegration agents,lubricants, sweetening agents, flavoring agents, dyes, such likematerials and combinations thereof, as would be known to one of ordinaryskill in the art (see, for example, Remington's Pharmaceutical Sciences,18th Ed. Mack Printing Company, 1990, pp. 1289-1329, incorporated hereinby reference). Except insofar as any conventional carrier isincompatible with the active ingredient, its use in the pharmaceuticalcompositions is contemplated.

The didemnin or didemnin derivative may comprise different types ofcarriers depending on whether it is to be administered in solid, liquidor aerosol form, and whether it need to be sterile for such routes ofadministration as injection. The present invention can be administeredintravenously, intradermally, transdermally, intrathecally,intraarterially, intraperitoneally, intranasally, intravaginally,intrarectally, topically, intramuscularly, subcutaneously, mucosally,orally, topically, locally, inhalation (e.g., aerosol inhalation),injection, infusion, continuous infusion, localized perfusion bathingtarget cells directly, via a catheter, via a lavage, in cremes, in lipidcompositions (e.g., liposomes), or by other method or any combination ofthe forgoing as would be known to one of ordinary skill in the art (see,for example, Remington's Pharmaceutical Sciences, 18th Ed. Mack PrintingCompany, 1990, incorporated herein by reference).

The didemnin or didemnin derivative may be formulated into a compositionin a free base, neutral or salt form. Pharmaceutically acceptable salts,include the acid addition salts, e.g., those formed with the free aminogroups of a proteinaceous composition, or which are formed withinorganic acids such as for example, hydrochloric or phosphoric acids,or such organic acids as acetic, oxalic, tartaric or mandelic acid.Salts formed with the free carboxyl groups can also be derived frominorganic bases such as for example, sodium, potassium, ammonium,calcium or ferric hydroxides; or such organic bases as isopropylamine,trimethylamine, histidine or procaine. Upon formulation, solutions willbe administered in a manner compatible with the dosage formulation andin such amount as is therapeutically effective. The formulations areeasily administered in a variety of dosage forms such as formulated forparenteral administrations such as injectable solutions, or aerosols fordelivery to the lungs, or formulated for alimentary administrations suchas drug release capsules and the like.

Further in accordance with the present invention, the composition of thepresent invention suitable for administration is provided in apharmaceutically acceptable carrier with or without an inert diluent.The carrier should be assimilable and includes liquid, semi-solid, i.e.,pastes, or solid carriers. Except insofar as any conventional media,agent, diluent or carrier is detrimental to the recipient or to thetherapeutic effectiveness of a the composition contained therein, itsuse in administrable composition for use in practicing the methods ofthe present invention is appropriate. Examples of carriers or diluentsinclude fats, oils, water, saline solutions, lipids, liposomes, resins,binders, fillers and the like, or combinations thereof. The compositionmay also comprise various antioxidants to retard oxidation of one ormore component. Additionally, the prevention of the action ofmicroorganisms can be brought about by preservatives such as variousantibacterial and antifungal agents, including but not limited toparabens (e.g., methylparabens, propylparabens), chlorobutanol, phenol,sorbic acid, thimerosal or combinations thereof.

In accordance with the present invention, the composition is combinedwith the carrier in any convenient and practical manner, i.e., bysolution, suspension, emulsification, admixture, encapsulation,absorption and the like. Such procedures are routine for those skilledin the art.

In a specific embodiment of the present invention, the composition iscombined or mixed thoroughly with a semi-solid or solid carrier. Themixing can be carried out in any convenient manner such as grinding.Stabilizing agents can be also added in the mixing process in order toprotect the composition from loss of therapeutic activity, i.e.,denaturation in the stomach. Examples of stabilizers for use in an thecomposition include buffers, amino acids such as glycine and lysine,carbohydrates such as dextrose, mannose, galactose, fructose, lactose,sucrose, maltose, sorbitol, mannitol, etc.

In further embodiments, the present invention may concern the use of apharmaceutical lipid vehicle compositions that include a didemnin ordidemnin derivative, one or more lipids, and an aqueous solvent. As usedherein, the term “lipid” will be defined to include any of a broad rangeof substances that is characteristically insoluble in water andextractable with an organic solvent. This broad class of compounds arewell known to those of skill in the art, and as the term “lipid” is usedherein, it is not limited to any particular structure. Examples includecompounds which contain long-chain aliphatic hydrocarbons and theirderivatives. A lipid may be naturally occurring or synthetic (i.e.,designed or produced by man). However, a lipid is usually a biologicalsubstance. Biological lipids are well known in the art, and include forexample, neutral fats, phospholipids, phosphoglycerides, steroids,terpenes, lysolipids, glycosphingolipids, glycolipids, sulphatides,lipids with ether and ester-linked fatty acids and polymerizable lipids,and combinations thereof. Of course, compounds other than thosespecifically described herein that are understood by one of skill in theart as lipids are also encompassed by the compositions and methods ofthe present invention.

One of ordinary skill in the art would be familiar with the range oftechniques that can be employed for dispersing a composition in a lipidvehicle. For example, the didemnin or didemnin derivative may bedispersed in a solution containing a lipid, dissolved with a lipid,emulsified with a lipid, mixed with a lipid, combined with a lipid,covalently bonded to a lipid, contained as a suspension in a lipid,contained or complexed with a micelle or liposome, or otherwiseassociated with a lipid or lipid structure by any means known to thoseof ordinary skill in the art. The dispersion may or may not result inthe formation of liposomes.

The actual dosage amount of a composition of the present inventionadministered to an animal patient can be determined by physical andphysiological factors such as body weight, severity of condition, thetype of disease being treated, previous or concurrent therapeuticinterventions, idiopathy of the patient and on the route ofadministration. Depending upon the dosage and the route ofadministration, the number of administrations of a preferred dosageand/or an effective amount may vary according to the response of thesubject. The practitioner responsible for administration will, in anyevent, determine the concentration of active ingredient(s) in acomposition and appropriate dose(s) for the individual subject.

In certain embodiments, pharmaceutical compositions may comprise, forexample, at least about 0.1% of an active compound. In otherembodiments, the an active compound may comprise between about 2% toabout 75% of the weight of the unit, or between about 25% to about 60%,for example, and any range derivable therein. Naturally, the amount ofactive compound(s) in each therapeutically useful composition may beprepared is such a way that a suitable dosage will be obtained in anygiven unit dose of the compound. Factors such as solubility,bioavailability, biological half-life, route of administration, productshelf life, as well as other pharmacological considerations will becontemplated by one skilled in the art of preparing such pharmaceuticalformulations, and as such, a variety of dosages and treatment regimensmay be desirable.

In other non-limiting examples, a dose may also comprise from about 1microgram/kg/body weight, about 5 microgram/kg/body weight, about 10microgram/kg/body weight, about 50 microgram/kg/body weight, about 100microgram/kg/body weight, about 200 microgram/kg/body weight, about 350microgram/kg/body weight, about 500 microgram/kg/body weight, about 1milligram/kg/body weight, about 5 milligram/kg/body weight, about 10milligram/kg/body weight, about 50 milligram/kg/body weight, about 100milligram/kg/body weight, about 200 milligram/kg/body weight, about 350milligram/kg/body weight, about 500 milligram/kg/body weight, to about1000 mg/kg/body weight or more per administration, and any rangederivable therein. In non-limiting examples of a derivable range fromthe numbers listed herein, a range of about 5 mg/kg/body weight to about100 mg/kg/body weight, about 5 microgram/kg/body weight to about 500milligram/kg/body weight, etc., can be administered, based on thenumbers described above.

D. Alimentary Compositions and Formulations

In preferred embodiments of the present invention, the composition(s)are formulated to be administered via an alimentary route. Alimentaryroutes include all possible routes of administration in which thecomposition is in direct contact with the alimentary tract.Specifically, the pharmaceutical compositions disclosed herein may beadministered orally, buccally, rectally, or sublingually. As such, thesecompositions may be formulated with an inert diluent or with anassimilable edible carrier, or they may be enclosed in hard- orsoft-shell gelatin capsule, or they may be compressed into tablets, orthey may be incorporated directly with the food of the diet.

In certain embodiments, the active compounds may be incorporated withexcipients and used in the form of ingestible tablets, buccal tables,troches, capsules, elixirs, suspensions, syrups, wafers, and the like(Mathiowitz et al., 1997; Hwang et al., 1998; U.S. Pat. Nos. 5,641,515;5,580,579 and 5,792,451, each specifically incorporated herein byreference in its entirety). The tablets, troches, pills, capsules andthe like may also contain the following: a binder, such as, for example,gum tragacanth, acacia, cornstarch, gelatin or combinations thereof; anexcipient, such as, for example, dicalcium phosphate, mannitol, lactose,starch, magnesium stearate, sodium saccharine, cellulose, magnesiumcarbonate or combinations thereof; a disintegrating agent, such as, forexample, corn starch, potato starch, alginic acid or combinationsthereof; a lubricant, such as, for example, magnesium stearate; asweetening agent, such as, for example, sucrose, lactose, saccharin orcombinations thereof; a flavoring agent, such as, for examplepeppermint, oil of wintergreen, cherry flavoring, orange flavoring, etc.When the dosage unit form is a capsule, it may contain, in addition tomaterials of the above type, a liquid carrier. Various other materialsmay be present as coatings or to otherwise modify the physical form ofthe dosage unit. For instance, tablets, pills, or capsules may be coatedwith shellac, sugar, or both. When the dosage form is a capsule, it maycontain, in addition to materials of the above type, carriers such as aliquid carrier. Gelatin capsules, tablets, or pills may be entericallycoated. Enteric coatings prevent denaturation of the composition in thestomach or upper bowel where the pH is acidic. See, e.g., U.S. Pat. No.5,629,001. Upon reaching the small intestines, the basic pH thereindissolves the coating and permits the composition to be released andabsorbed by specialized cells, e.g., epithelial enterocytes and Peyer'spatch M cells. A syrup of elixir may contain the active compound sucroseas a sweetening agent methyl and propylparabens as preservatives, a dyeand flavoring, such as cherry or orange flavor. Of course, any materialused in preparing any dosage unit form should be pharmaceutically pureand substantially non-toxic in the amounts employed. In addition, theactive compounds may be incorporated into sustained-release preparationand formulations.

For oral administration the compositions of the present invention mayalternatively be incorporated with one or more excipients in the form ofa mouthwash, dentifrice, buccal tablet, oral spray, or sublingualorally-administered formulation. For example, a mouthwash may beprepared incorporating the active ingredient in the required amount inan appropriate solvent, such as a sodium borate solution (Dobell'sSolution). Alternatively, the active ingredient may be incorporated intoan oral solution such as one containing sodium borate, glycerin andpotassium bicarbonate, or dispersed in a dentifrice, or added in atherapeutically-effective amount to a composition that may includewater, binders, abrasives, flavoring agents, foaming agents, andhumectants. Alternatively the compositions may be fashioned into atablet or solution form that may be placed under the tongue or otherwisedissolved in the mouth.

Additional formulations which are suitable for other modes of alimentaryadministration include suppositories. Suppositories are solid dosageforms of various weights and shapes, usually medicated, for insertioninto the rectum. After insertion, suppositories soften, melt or dissolvein the cavity fluids. In general, for suppositories, traditionalcarriers may include, for example, polyalkylene glycols, triglyceridesor combinations thereof. In certain embodiments, suppositories may beformed from mixtures containing, for example, the active ingredient inthe range of about 0.5% to about 10%, and preferably about 1% to about2%.

E. Parenteral Compositions and Formulations

In further embodiments, the composition may be administered via aparenteral route. As used herein, the term “parenteral” includes routesthat bypass the alimentary tract. Specifically, the pharmaceuticalcompositions disclosed herein may be administered for example, but notlimited to intravenously, intradermally, intramuscularly,intraarterially, intrathecally, subcutaneous, or intraperitoneally U.S.Pat. Nos. 6,7537,514, 6,613,308, 5,466,468, 5,543,158; 5,641,515; and5,399,363 (each specifically incorporated herein by reference in itsentirety).

Solutions of the active compounds as free base or pharmacologicallyacceptable salts may be prepared in water suitably mixed with asurfactant, such as hydroxypropylcellulose. Dispersions may also beprepared in glycerol, liquid polyethylene glycols, and mixtures thereofand in oils. Under ordinary conditions of storage and use, thesepreparations contain a preservative to prevent the growth ofmicroorganisms. The pharmaceutical forms suitable for injectable useinclude sterile aqueous solutions or dispersions and sterile powders forthe extemporaneous preparation of sterile injectable solutions ordispersions (U.S. Pat. No. 5,466,468, specifically incorporated hereinby reference in its entirety). In all cases the form must be sterile andmust be fluid to the extent that easy injectability exists. It must bestable under the conditions of manufacture and storage and must bepreserved against the contaminating action of microorganisms, such asbacteria and fungi. The carrier can be a solvent or dispersion mediumcontaining, for example, water, ethanol, polyol (i.e., glycerol,propylene glycol, and liquid polyethylene glycol, and the like),suitable mixtures thereof, and/or vegetable oils. Proper fluidity may bemaintained, for example, by the use of a coating, such as lecithin, bythe maintenance of the required particle size in the case of dispersionand by the use of surfactants. The prevention of the action ofmicroorganisms can be brought about by various antibacterial andantifungal agents, for example, parabens, chlorobutanol, phenol, sorbicacid, thimerosal, and the like. In many cases, it will be preferable toinclude isotonic agents, for example, sugars or sodium chloride.Prolonged absorption of the injectable compositions can be brought aboutby the use in the compositions of agents delaying absorption, forexample, aluminum monostearate and gelatin.

For parenteral administration in an aqueous solution, for example, thesolution should be suitably buffered if necessary and the liquid diluentfirst rendered isotonic with sufficient saline or glucose. Theseparticular aqueous solutions are especially suitable for intravenous,intramuscular, subcutaneous, and intraperitoneal administration. In thisconnection, sterile aqueous media that can be employed will be known tothose of skill in the art in light of the present disclosure. Forexample, one dosage may be dissolved in isotonic NaCl solution andeither added hypodermoclysis fluid or injected at the proposed site ofinfusion, (see for example, “Remington's Pharmaceutical Sciences” 15thEdition, pages 1035-1038 and 1570-1580). Some variation in dosage willnecessarily occur depending on the condition of the subject beingtreated. The person responsible for administration will, in any event,determine the appropriate dose for the individual subject. Moreover, forhuman administration, preparations should meet sterility, pyrogenicity,general safety and purity standards as required by FDA Office ofBiologics standards.

Sterile injectable solutions are prepared by incorporating the activecompounds in the required amount in the appropriate solvent with variousof the other ingredients enumerated above, as required, followed byfiltered sterilization. Generally, dispersions are prepared byincorporating the various sterilized active ingredients into a sterilevehicle which contains the basic dispersion medium and the requiredother ingredients from those enumerated above. In the case of sterilepowders for the preparation of sterile injectable solutions, thepreferred methods of preparation are vacuum-drying and freeze-dryingtechniques which yield a powder of the active ingredient plus anyadditional desired ingredient from a previously sterile-filteredsolution thereof. A powdered composition is combined with a liquidcarrier such as, e.g., water or a saline solution, with or without astabilizing agent.

F. Miscellaneous Pharmaceutical Compositions and Formulations

In other preferred embodiments of the invention, the active compound maybe formulated for administration via various miscellaneous routes, forexample, topical (i.e., transdermal) administration, mucosaladministration (intranasal, vaginal, etc.) and/or inhalation.

Pharmaceutical compositions for topical administration may include theactive compound formulated for a medicated application such as anointment, paste, cream or powder. Ointments include all oleaginous,adsorption, emulsion and water-solubly based compositions for topicalapplication, while creams and lotions are those compositions thatinclude an emulsion base only. Topically administered medications maycontain a penetration enhancer to facilitate adsorption of the activeingredients through the skin. Suitable penetration enhancers includeglycerin, alcohols, alkyl methyl sulfoxides, pyrrolidones andluarocapram. Possible bases for compositions for topical applicationinclude polyethylene glycol, lanolin, cold cream and petrolatum as wellas any other suitable absorption, emulsion or water-soluble ointmentbase. Topical preparations may also include emulsifiers, gelling agents,and antimicrobial preservatives as necessary to preserve the activeingredient and provide for a homogenous mixture. Transdermaladministration of the present invention may also comprise the use of a“patch”. For example, the patch may supply one or more active substancesat a predetermined rate and in a continuous manner over a fixed periodof time.

In certain embodiments, the pharmaceutical compositions may be deliveredby eye drops, intranasal sprays, inhalation, and/or other aerosoldelivery vehicles. Methods for delivering compositions directly to thelungs via nasal aerosol sprays has been described e.g., in U.S. Pat.Nos. 5,756,353 and 5,804,212 (each specifically incorporated herein byreference in its entirety). Likewise, the delivery of drugs usingintranasal microparticle resins (Takenaga et al., 1998) andlysophosphatidyl-glycerol compounds (U.S. Pat. No. 5,725,871,specifically incorporated herein by reference in its entirety) are alsowell-known in the pharmaceutical arts. Likewise, transmucosal drugdelivery in the form of a polytetrafluoroetheylene support matrix isdescribed in U.S. Pat. No. 5,780,045 (specifically incorporated hereinby reference in its entirety).

The term aerosol refers to a colloidal system of finely divided solid ofliquid particles dispersed in a liquefied or pressurized gas propellant.The typical aerosol of the present invention for inhalation will consistof a suspension of active ingredients in liquid propellant or a mixtureof liquid propellant and a suitable solvent. Suitable propellantsinclude hydrocarbons and hydrocarbon ethers. Suitable containers willvary according to the pressure requirements of the propellant.Administration of the aerosol will vary according to subject's age,weight and the severity and response of the symptoms.

IX. COMBINATION THERAPY

In some embodiments, in order to increase the effectiveness of adidemnin or didemnin derivative, it may be desirable to combine thesecompositions with other agents effective in the treatment ofhyperproliferative disease, such as anti-cancer agents. An “anti-cancer”agent is capable of negatively affecting cancer in a subject, forexample, by killing cancer cells, inducing apoptosis in cancer cells,reducing the growth rate of cancer cells, reducing the incidence ornumber of metastases, reducing tumor size, inhibiting tumor growth,reducing the blood supply to a tumor or cancer cells, promoting animmune response against cancer cells or a tumor, preventing orinhibiting the progression of cancer, or increasing the lifespan of asubject with cancer. More generally, these other compositions would beprovided in a combined amount effective to kill or inhibit proliferationof the cell. This process may involve contacting the cells with thedidemnin or didemnin derivative and the agent(s) or multiple factor(s)at the same time. This may be achieved by contacting the cell with asingle composition or pharmacological formulation that includes bothagents, or by contacting the cell with two distinct compositions orformulations, at the same time, wherein one composition includes theexpression construct and the other includes the second agent(s).

Tumor cell resistance to chemotherapy and radiotherapy agents representsa major problem in clinical oncology. One goal of current cancerresearch is to find ways to improve the efficacy of chemo- andradiotherapy by combining it with other therapy. In the context of thepresent invention, it is contemplated that a didemnin or didemninderivative could be used in conjunction with chemotherapeutic,radiotherapeutic, or immunotherapeutic intervention, for example.

The didemnin or didemnin derivative therapy may precede or follow theother agent treatment by intervals ranging from minutes to weeks. Inembodiments where the other agent and expression construct are appliedseparately to the cell, one would generally ensure that a significantperiod of time did not expire between the time of each delivery, suchthat the agent and didemnin or didemnin derivative would still be ableto exert an advantageously combined effect on the cell. In suchinstances, it is contemplated that one may contact the cell with bothmodalities within about 12-24 h of each other and, more preferably,within about 6-12 h of each other. In some situations, it may bedesirable to extend the time period for treatment significantly,however, where several d (2, 3, 4, 5, 6 or 7) to several wk (1, 2, 3, 4,5, 6, 7 or 8) lapse between the respective administrations.

Various combinations may be employed, didemnin or didemnin derivativetherapy is “A” and the secondary agent, such as radio- or chemotherapy(for example), is “B”:

A/B/A B/A/B B/B/A A/A/B A/B/B B/A/A A/B/B/B B/A/B/B

B/B/B/A B/B/A/B A/A/B/B A/B/A/B A/B/B/A B/B/A/A

B/A/B/A B/A/A/B A/A/A/B B/A/A/A A/B/A/A A/A/B/A

Administration of the therapeutic didemnin or didemnin derivative of thepresent invention to a patient will follow general protocols for theadministration of chemotherapeutics, taking into account the toxicity,if any, of the vector. It is expected that the treatment cycles would berepeated as necessary. It also is contemplated that various standardtherapies, as well as surgical intervention, may be applied incombination with the described hyperproliferative cell therapy.

A. Chemotherapy

Cancer therapies also include a variety of combination therapies withboth chemical and radiation based treatments. Combination chemotherapiesinclude, for example, cisplatin (CDDP), carboplatin, procarbazine,mechlorethamine, cyclophosphamide, camptothecin, ifosfamide, melphalan,chlorambucil, busulfan, nitro surea, dactinomycin, daunorubicin,doxorubicin, bleomycin, plicomycin, mitomycin, etoposide (VP16),tamoxifen, raloxifene, estrogen receptor binding agents, taxol,gemcitabien, navelbine, farnesyl-protein tansferase inhibitors,transplatinum, 5-fluorouracil, vincristin, vinblastin and methotrexate,or any analog or derivative variant of the foregoing.

B. Radiotherapy

Other factors that cause DNA damage and have been used extensivelyinclude what are commonly known as 7-rays, X-rays, and/or the directeddelivery of radioisotopes to tumor cells. Other forms of DNA damagingfactors are also contemplated such as microwaves and UV-irradiation. Itis most likely that all of these factors effect a broad range of damageon DNA, on the precursors of DNA, on the replication and repair of DNA,and on the assembly and maintenance of chromosomes. Dosage ranges forX-rays range from daily doses of 50 to 200 roentgens for prolongedperiods of time (3 to 4 wk), to single doses of 2000 to 6000 roentgens.Dosage ranges for radioisotopes vary widely, and depend on the half-lifeof the isotope, the strength and type of radiation emitted, and theuptake by the neoplastic cells.

The terms “contacted” and “exposed,” when applied to a cell, are usedherein to describe the process by which a therapeutic construct and achemotherapeutic or radiotherapeutic agent are delivered to a targetcell or are placed in direct juxtaposition with the target cell. Toachieve cell killing or stasis, both agents are delivered to a cell in acombined amount effective to kill the cell or prevent it from dividing.

C. Immunotherapy

Immunotherapeutics, generally, rely on the use of immune effector cellsand molecules to target and destroy cancer cells. The immune effectormay be, for example, an antibody specific for some marker on the surfaceof a tumor cell. The antibody alone may serve as an effector of therapyor it may recruit other cells to actually effect cell killing. Theantibody also may be conjugated to a drug or toxin (chemotherapeutic,radionuclide, ricin A chain, cholera toxin, pertussis toxin, etc.) andserve merely as a targeting agent. Alternatively, the effector may be alymphocyte carrying a surface molecule that interacts, either directlyor indirectly, with a tumor cell target. Various effector cells includecytotoxic T cells and NK cells.

Immunotherapy, thus, could be used as part of a combined therapy, inconjunction with didemnin or didemnin derivative therapy. The generalapproach for combined therapy is discussed below. Generally, the tumorcell must bear some marker that is amenable to targeting, i.e., is notpresent on the majority of other cells. Many tumor markers exist and anyof these may be suitable for targeting in the context of the presentinvention. Common tumor markers include carcinoembryonic antigen,prostate specific antigen, urinary tumor associated antigen, fetalantigen, tyrosinase (p97), gp68, TAG-72, HMFG, Sialyl Lewis Antigen,MucA, MucB, PLAP, estrogen receptor, laminin receptor, erb B and p155.

D. Genes

In yet another embodiment, the secondary treatment is a gene therapy inwhich a therapeutic polynucleotide is administered before, after, or atthe same time as the didemnin or didemnin derivative. Delivery ofdidemnin or a didemnin derivative with a vector encoding one of atherapeutic gene product will have a combined anti-hyperproliferativeeffect on target tissues. Alternatively, a single vector encoding bothgenes may be used. A variety of proteins are encompassed within theinvention, including inducers of cellular proliferation, inhibitors ofcellular proliferation, or regulators of programmed cell death, forexample.

E. Surgery

Approximately 60% of persons with cancer will undergo surgery of sometype, which includes preventative, diagnostic or staging, curative andpalliative surgery. Curative surgery is a cancer treatment that may beused in conjunction with other therapies, such as the treatment of thepresent invention, chemotherapy, radiotherapy, hormonal therapy, genetherapy, immunotherapy and/or alternative therapies.

Curative surgery includes resection in which all or part of canceroustissue is physically removed, excised, and/or destroyed. Tumor resectionrefers to physical removal of at least part of a tumor. In addition totumor resection, treatment by surgery includes laser surgery,cryosurgery, electrosurgery, and miscopically controlled surgery (Mohs'surgery). It is further contemplated that the present invention may beused in conjunction with removal of superficial cancers, precancers, orincidental amounts of normal tissue.

Upon excision of part of all of cancerous cells, tissue, or tumor, acavity may be formed in the body. Treatment may be accomplished byperfusion, direct injection or local application of the area with anadditional anti-cancer therapy. Such treatment may be repeated, forexample, every 1, 2, 3, 4, 5, 6, or 7 days, or every 1, 2, 3, 4, and 5weeks or every 1, 2, 3, 4, 5, 6, 7, 8, 9, 10, 11, or 12 months. Thesetreatments may be of varying dosages as well.

F. Other Agents

It is contemplated that other agents may be used in combination with thepresent invention to improve the therapeutic efficacy of treatment.These additional agents include immunomodulatory agents, agents thataffect the upregulation of cell surface receptors and GAP junctions,cytostatic and differentiation agents, inhibitors of cell adehesion, oragents that increase the sensitivity of the hyperproliferative cells toapoptotic inducers. Immunomodulatory agents include tumor necrosisfactor; interferon alpha, beta, and gamma; IL-2 and other cytokines;F42K and other cytokine analogs; or MIP-1, MIP-1beta, MCP-1, RANTES, andother chemokines. It is further contemplated that the upregulation ofcell surface receptors or their ligands such as Fas/Fas ligand, DR4 orDR5/TRAIL would potentiate the apoptotic inducing abilities of thepresent invention by establishment of an autocrine or paracrine effecton hyperproliferative cells. Increases intercellular signaling byelevating the number of GAP junctions would increase theanti-hyperproliferative effects on the neighboring hyperproliferativecell population. In other embodiments, cytostatic or differentiationagents can be used in combination with the present invention to improvethe anti-hyerproliferative efficacy of the treatments. Inhibitors ofcell adehesion are contemplated to improve the efficacy of the presentinvention. Examples of cell adhesion inhibitors are focal adhesionkinase (FAKs) inhibitors and Lovastatin. It is further contemplated thatother agents that increase the sensitivity of a hyperproliferative cellto apoptosis, such as the antibody c225, could be used in combinationwith the present invention to improve the treatment efficacy.

Hormonal therapy may also be used in conjunction with the presentinvention or in combination with any other cancer therapy previouslydescribed. The use of hormones may be employed in the treatment ofcertain cancers such as breast, prostate, ovarian, or cervical cancer tolower the level or block the effects of certain hormones such astestosterone or estrogen. This treatment is often used in combinationwith at least one other cancer therapy as a treatment option or toreduce the risk of metastases.

X. KITS OF THE INVENTION

Any of the compositions described herein may be comprised in a kit,including, for example, one or more bacteria, media reagents, and soforth. In some embodiments, one or more reagents to assist infermentation of didemnin in bacteria is provided in a kit. In certainaspects, one or more compounds for use in preparing a didemninderivative from an endogenously produced Tistrella didemnin is includedin the kit.

The kits may comprise a suitably aliquoted composition of the presentinvention. The components of the kits may be packaged either in aqueousmedia or in lyophilized form, where appropriate. The container means ofthe kits will generally include at least one vial, test tube, flask,bottle, syringe or other container means, into which a component may beplaced, and preferably, suitably aliquoted. Where there are more thanone component in the kit, the kit also will generally contain a second,third or other additional container into which the additional componentsmay be separately placed. However, various combinations of componentsmay be comprised in a vial. The kits of the present invention also willtypically include a means for containing the kit component(s) in closeconfinement for commercial sale. Such containers may include injectionor blow molded plastic containers into which the desired vials areretained.

However, the components of the kit may be provided as dried powder(s).When reagents and/or components are provided as a dry powder, the powdercan be reconstituted by the addition of a suitable solvent. It isenvisioned that the solvent may also be provided in another containermeans.

EXAMPLES

The following examples are included to demonstrate preferred embodimentsof the invention. It should be appreciated by those of skill in the artthat the techniques disclosed in the examples which follow representtechniques discovered by the inventor to function well in the practiceof the invention, and thus can be considered to constitute preferredmodes for its practice. However, those of skill in the art should, inlight of the present disclosure, appreciate that many changes can bemade in the specific embodiments which are disclosed and still obtain alike or similar result without departing from the spirit and scope ofthe invention.

Example 1 Exemplary Methods Strain, Fermentation and Isolation ofDidemnin Compounds

Tistrella mobilis was isolated from seawater collected from the Red Seaduring a 2009 research cruise. Its crude extract showed remarkablecytotoxicity on HeLa cells in a bioactive compound screening of Red Seabacteria. T. mobilis was grown on GYP medium [10 g of glucose/4 g ofyeast extract/2 g of peptone/17 g of sea salts/1 liter of deionizedwater] using 50-liter stirred fermenters at 25° C. for 72 h. At the endof fermentation, ethyl acetate was added to the culture to extract themetabolites. The crude extract was then fractionated by reverse-phaseC18 liquid chromatography and eluted with increasing amounts of methanolin water. The active fraction was further purified by semi-preparativereverse-phase HPLC with 63% acetonitrile in water at a flow rate of 3milliliter per minute. Didemnin B and nordidemnin B were eluted at 25and 22 minute respectively.

Genome Sequencing, Annotation, and Analysis

The nucleotide sequence of the T. mobilis genome was determined by usinga massively parallel pyrosequencing technology (Roche 454 GS FLX). 112contigs (>500 bp) with a total size of 6.4 Mb were assembled from315,496 reads (average length of 334 bp) using Newbler software of the454 suite package, providing an 18.4-fold coverage. In addition,2,625,640 sequences with average length 115 bps of mate-pair produced bythe Illumina sequencing system were mapped to the genome sequence topromote sequence quality and construct a scaffold. All the contigrelationships within scaffolds were validated by PCR, and therelationship among scaffolds was determined by multiplex PCR. Gaps werefilled by sequencing PCR products. The final sequence assembly wascarried out using phred/phrap/consed package (see the World Wide websiteof the Genome Center at the University of Washington), and the lowsequence quality region was resequenced. The final error rate of genomesequence was 0.28 per 100,000 bases.

Protein-coding sequences (CDS) were determined by combining theprediction results of Glimmer 3.02 and Z-Curve programs. Functionalannotation of CDS were performed by searching the NCBI non-redundantprotein database and KEGG protein database. tRNA genes were predictedwith tRNAScan-SE (v1.23). Protein domain prediction and COG assignmentwere performed by RPS-BLAST using the NCBI CDD library.

in Silico Analysis of Didemnin Biosynthetic Gene Cluster and Other NRPSin T. mobilis Genome

The roles of the proteins, in embodiments of the invention, in thedidemnin gene cluster were assigned using protein-protein BLAST and Pfamanalysis. The NRPS A domain specificity was predicted using onlineprogram NRPSpredictor (Rausch 2005). The nucleotide sequences of thegene cluster can be deposited at GenBank®, for example.

Example 2 General Genome Feature of Tistrella mobilis and AssociatedNRPS Gene Clusters

Isolation of Didemnins from Tistrella mobilis

The bacterium was noted for its astonishing cytotoxic activity during ascreening program of bacteria isolated from the Red Sea for cytotoxicactivity. Its ethyl acetate crude extract of culture broth could killthe HeLa cells at a concentration lower than 1 ng/ml. The bacterium wasthen fermented to 70 l and the ethyl acetate extract was subjected toreverse phase column chromatography using 15%, 30%, 45%, 60%, 70%, 90%and 100% methanol in water, respectively. Fractions eluted using 60% and70% showed strong cytotoxic activity using HCT-116 human colon carcinomacell. One active fraction was identified with peptide signals, using¹H-NMR analysis and MS analysis to reveal two separate peaks with [M+H]of 1098.7 and 1112.7, respectively (FIG. 2). We purified both compoundsusing semi-preparative HPLC and both of them showed strong cytotoxicactivity at a concentration of 0.074 ng/ml. Using NMR, MS and theAntiMarin database, we identified these two compounds to be didemnin Band nordidemnin B. A pure form didemnin B was acquired from Prof. ChrisIreland at the University of California, Santa Cruz, who with coworkers,isolated it from the tunicate Trididemnum solidum. This sample was usedto compare its NMR spectral data with the present data and to furtherconfirm that the compound isolated from T. mobilis was didemnin B.Moreover, other fractions from T. mobilis also exhibited similarbioactivity and LC-MS analysis showed the presence of other didemnins,including didemnin A, and didemnin C, at trace amounts. This isconsistent with the fact that didemnin B and nordidemnin B were the mostabundant in the Trididemnum tunicates.

Biosynthetic Pathways of Didemnins

The putative biosynthetic gene cluster for didemnins contains 26 ORFs(Table 2).

TABLE 2 Deduced functions of the proteins in the didemnin biosyntheticgene cluster Amino Identity/ GenBank gene acid aa) Sequencesimilarity/organism Proposed function similarity accession no. orf1 261thioesterase, Actinomadura kijaniata Thioesterase 41%, 54% ACB46473 orf2483 band 7 protein, Methylobacter 50%, 68% ZP_07653046 tundripaludumSV96 orf3 560 cyclic peptide transporter, transporter 48%, 68%ZP_07653047 Methylobacter tundripaludum SV96 orf4 70 No hits orf5 190GTPase domain-containing protein, 39%, 57% ZP_07653048 Methylobactertundripaludum SV96 orf6 987 hydrophobic/amphiphilic exporter-1,Resistance 38%, 56% YP_003450508 Azospirillum sp. B510 orf7 377secretion protein, Azospirillum sp. Resistance 28%, 47% YP_003450507B510 didA 2123 OciA protein, Planktothrix rubescens NRPS (C A T C A 29%,40% CAQ48254 NIVA-CYA 98 T) didB 1796 linear gramicidin synthetasesubunit D, NPRS (C A KR T) 36%, 48% ZP_01459555 Stigmatella aurantiacaDW4/3-1 didC 1330 NRPS, Myxococcus xanthus DK 1622 NRPS (C A T C*) 41%,52% YP_632257 didD 3853 amino acid adenylation domain protein, NRPS (C AM T 39%, 50% ZP_07603194 Streptomyces violaceusniger Tu 4113 C* C A T CA T) didE 1705 amino acid adenylation domain protein, NRPS/PKS (KS 36%,53% ZP_07325073 Acetivibrio cellulolyticus CD2 KR T C) didF 1613 HctF,Lyngbya majuscula NRPS (A* A KR 35%, 52% AAY42398 T) didG 1413 NRPS/PKS,Amycolatopsis PKS (KS M T) 46%, 56% YP_003765866 mediterranei U32 didH1286 NRPS/PKS, Myxococcus xanthus DK NRPS (C A T C*) 39%, 53% YP_6319611622 didI 873 NRPS, Myxococcus xanthus DK 1622 NRPS (C A T) 39%, 52%YP_632257 didJ 2163 amino acid adenylation domain protein, NRPS (C A M MT 36%, 53% ZP_08431746 Lyngbya majuscula 3L TE) orf8 77 MbtHdomain-containing protein, MbtH-like protein 80%, 89% YP_001542806Herpetosiphon aurantiacus ATCC 23779 orf9 68 hypothetical protein,Acidovorax sp. 79%, 91% YP_986866 JS42 orf10 45 No hits orf11 60 No hitsorf12 190 hypothetical protein, Acidovorax sp. 94%, 97% YP_986861 JS42orf13 75 hypothetical protein, Acidovorax sp. 98%, 100% YP_004387524JS42 orf14 324 CAAX amino terminal protease family, 30%, 49% ZP_05035401Synechococcus sp. PCC 7335 orf15 398 cyanate transport system protein,40%, 52% ZP_07265073 Pseudomonas syringae pv. syringae 642 orf16 255GntR family transcriptional regulator, Regulation 39%, 55% NP_903400Chromobacterium violaceum ATCC 12472

Ten of them, didA to didJ, are most likely to be involved in thebiosynthesis of didemnins (FIG. 3). All of them encode for non-ribosomalpeptide synthetase (NRPS) except didE and didG. Most NRPS are modularenzymes that use an assembly line strategy to synthesize nonribosomalpeptides. A typical module in the NRPS assembly line usually has aminimal of three domains: the adenylation domain (A) activates aspecific amino acid via the formation of an acyl-AMP; the activated formof the amino acid is then loaded to an adjacent thiolation (T) domain;and the condensation (C) domain catalyzes peptide bond formation. Inaddition to these three basic domains, different tailoring domains, suchas ketoreductase (KR) domains or methyltransferase (MT) domains, aresometimes present in NRPS modules. These tailoring domains makemodification of the amino acids and they are one of the main reasonsthat many non-ribosomal peptides contain non-proteinogenic amino acidmomoners. Most NRPS generally follow a colinearity principle wherein theA domain specificity and placement within the NRPS assembly linedictates the sequence of the monomers in the nonribosomal peptide(Marahiel et al. 1997; Fischbach and Walsh 2006). Analysis of thesubstrate specificity of the A domains by NPRSpredictor cansubstantially help match the A domains with the corresponding amino acidsubstrate. There are a total of 11 A domains in the eight NRPS in thedidemnin gene clusters and it is the specificity of these A domains thatleads us to postulate that this gene cluster is responsible for didemninsynthesis (Table 3).

Adenyla- tion 10 amino  Identity, protein domain acid code Substrate %DidA A₁ DAWQFGLIDK Glutamine 100 (SEQ ID NO: 1) DidA A₂ DAWQFGLIDKGlutamine 100 (SEQ ID NO: 1) DidB A₃ DHPWIAETVK (pyruvic  (SEQ ID NO: 2)acid) DidC A₄ DVQFAAQVVK Proline 90 (SEQ ID NO: 3) DidD A₅ DAWFLGHVVKLeucine 90 (SEQ ID NO: 4) DidD A₆ DFWNIGMVHK Threonine 100(SEQ ID NO: 5) DidD A₇ DAFFLGITFK Ile 90 (SEQ ID NO: 6) DidF A₈ No code(2-oxoiso- valeric acid) DidH A₉ DAWFLGNVVK Leucine  100 (SEQ ID NO: 7)DidI A₁₀ DVQFAAQVVK Proline  90 (SEQ ID NO: 8) DidJ A₁₁ DASTLAAVCKTyrosine 90 (SEQ ID NO: 9)

For example, the inventors found that in at least certain aspects the Adomain of DidC activates proline while the first two A domains in DidDactivate leucine and threonine, respectively. A fragment of the didemninB happens to contain these three amino acids in the same sequence asthey are activated by the corresponding A domains. Moreover, the leucinein the didemnin B has an N-terminal methyl group and this matches wellwith the fact that there is also a methylation domain right next to theA domain activating leucine. In a similar way, the inventors found the Adomains in DidH, DidI and DidJ in certain embodiments activate leucine,proline and tyrosine. DidI contains a MT tailoring domain that willconvert proline into N-methyl proline while DidJ contains two MTdomains, one N-methyltransferase and one O-methyltransferase, which mayrespectively add a methyl group on the amine and hydroxyl group of thetyrosine. Leucine, N-methyl proline and N,O-dimethyl tyrosine togethermake another fragment of the didemnin B.

The remaining monomers of the didemnin B are a lactic acid (Lac), anisostatine (Ist) and an α-(α-hydroxyisovaleryl)-propionic acid (Hip).According to the colinearity rule, DidB, which contains four domains(C-A-KR-T), should be the module that feeds the lactic acid intodidemnin B. Although NRPSpredictor fails to predict a reliable substratefor the didB A domain, BLAST search of its sequence shows that it ishighly similar to one of the A domains found in the valinomycin genecluster. According to Chen (2006), this A domain may activate pyruvicacid. The pyruvic acid is then reduced to lactic acid, which is also amonomer of valinomycin. Therefore, the A domain in DidB may firstactivate pyruvic acid and the KR domain then comes to reduce the pyruvicacid to lactic acid, which is exactly the same scenario in thevancomycin gene cluster. The third A domain in DidD is most likely toactivate isoleucine according to the code specificity analysis.Downstream to DidD is a hybrid polyketide sythetase/nonribosomal proteinsynthetase (PKS/NRPS) protein DidE comprising of a Ketosynthase domain(KS), a Ketoreductase (KR), a thiolation domain (T) and a condensationdomain (C). It can be hypothesized that the after isoleucine isincorporated, the intermediate molecule bearing the isoleucine goesthrough a round of PKS reaction which contains two steps: 1) addition ofa 2-carbon unit as a common PKS chain elongation step possibly using amalonyl-CoA as a substrate, and 2) the carbonyl group of the isoleucineis reduced to a hydroxyl group. This series of reactions can thenexplain the existence of the monomer Ist in the didemnin B molecule.However, the above PKS system features as the “AT-less” PKS system as noacyltransferase can be found in this module. Moreover, no stand alone ATgenes can be found in the entire didemnin gene cluster and its vicinity,which is a very rare phenomenon in “AT-less” PKS systems as normally atleast one stand alone AT gene can be found in the vicinity thatfunctions in trans to catalyze the activation of the substrate, whichmost of the time would be a malonyl-CoA. Nevertheless, in certainaspects the Ist monomer is added through the joint effect of a NRPSmodule and a hybrid PKS/NPRS module. It is unlikely that a single stepin which an A domain specifically activates Ist catalyzes the reactionas predicted by Salomon et al. (2004). Downstream of the didD gene aredidE and didF, the former is a NRPS gene while the latter is a PKS gene.The A domain in DidE does not have a code specified for any knownsubstrate, yet its amino acid sequence shares 43% identity and 60%similarity with that of the first adenylation domain of HetE of thecyanobacterium Lyngbya majuscule that produces the cyclic peptidehectochlorin. Ramaswamy et al. (2007) used the ATP-PPi exchange activityassay to demonstrate that the first A domain of HetE can activate2-oxo-isovaleric acid. Therefore the A domain in DidE may first activatethe 2-oxo-isovaleric acid, which is then reduced in situ by a KR domainin DidE to 2-hydroxyisovaleric acid (2-Hiv). The KS domain of DidF thenadds a 2-carbon unit to the C-terminus of Hiv and the MT domain in DidFcatalyzes the addition of a methyl group. The net outcome of thiscascade of reactions is the incorporation of the monomer Hip intodidemnin molecules (FIG. 4). This is very similar to the scenario of theaddition of Ist in which a joint effort of two proteins eventuallycatalyze the addition of the necessary monomer into the final molecule.Finally, in some aspects the thioesterase domain of the DidJ catalyzesthe macrocyclization between the hydroxyl group on the side chain ofthreonine residue and the thioester group of T domain bound tyrosineresidue, resulting in the release of the mature cyclic depsipeptidedidemnin compounds. Therefore, in certain embodiments of the inventiondidB to didJ are responsible for the biosynthesis of didemnin B (FIG.5).

Apart from didemnin B, the inventors also isolated nordidemnin B, whichcontains the monomer norstatine, a very similar derivative ofisostatine. Since the A domains usually have some flexibility inactivating similar substrates, valine, instead of isoleucine, may alsobe activated by the third A domain of DidD and together with DidE,norstatine may therefore be incorporated to produce nordidemnin B, insome embodiments. Upstream of didB, didA encodes two modules of NRPSwith the C A TC AT domain organization. Both A domains have specifiedcodes to activate glutamine. There are some other didemnins which havemultiple glutamine residues preceding the lactic residue, such asdidemnin X (FIG. 1) and didemnin Y. In some cases with didA, this genecluster produces didemnin X or didemnin Y. Similar to plipastatin,didemnin X and didemnin Y are both N-acylated. It has been reported thatthe first C domain in the initiation module may catalyze the N-acylationof the first amino acid in some NRPS systems (Imker et al. 2010).However, further studies are performed to characterize this. Apart fromdidemnin B and nordidemnin B, the inventors also used UPLC-HRMS todetect many other didemnin derivatives, such as didemnin A and didemninC by growing T. mobilis in different culture media or with differentculture durations.

Additional ORFs in the Didemnin Gene Cluster

Besides the synthesis of didemnins, other genes that are possiblyinvolved in the regulation, resistance and transport of the didemninsare also found in the didemnin gene cluster. Orf1, which encodes a typeII thioesterase, may regenerate the misprimed NPRS which are inactive toensure the NRPS assembly line is functional. Orf3, orf6 and orf7 allencode proteins related to the transport and secretion of the cyclicpeptide and they may be responsible for the secretion of didemnins. Assecretion and self-resistance are often related, they may also functionas self-resistance genes to protect the producer. Orf8 encodes anMbtH-like protein that is a common resident of various NRPS systems.Although the precise function of MbtH-like proteins in NRPS systems hasnot been revealed, in specific aspects they play an important role inthe production of many non-ribosomal peptides as indicated by Lautru etal., (2007). Orf16 encodes a Gnt-family transcription factor and this isthe only transcription-related gene that can be found in the vicinity ofthe didemnin biosynthetic gene cluster. As GntR family transcriptionfactors usually, though not always act as repressive regulators (Chen etal, 2010), orf16 may participate in the regulation of the biosynthesisof didemnins. It is also reasonable as overproduction of complicateddidemnins could cause a huge waste of energy for the bacterium. Althoughdidemnins have been reported for their potent cytotoxic, antivirus,immunosuppressive activities, the real ecological function of thesecompounds for the producing bacterium is unknown. To synthesize suchcomplicated molecules, the bacterium has to invest considerable energywith good reason.

Manipulation of the Didemnin Gene Cluster

There are huge incentives to manipulate the didemnin gene cluster asdidemnins other than didemnin B or nordidemnin B could be produced fromthis gene cluster. For example, there might be a way to producedehydrodidemnin B (Aplidine), which is a promising antitumor drugcurrently in clinical trials (Le Tourneau et al. 2010, Mateos et al.2010). The only difference between dehydrodidemnin B and didemnin B isthat the former has a pyruvic acid monomer while the latter has a lacticacid. If the ketoreductase domain in didB can be knocked out, then theincorporated pyruvic acid will not be reduced to lactic acid andconsequently, dehydrodidemnin B may be expected to be produced. Uponconstruction of the corresponding deletion mutant, there may be analternative way to produce dehydrodidemnin B other than chemicalsynthesis, and the cost could be substantially reduced. In addition, asmore than 10 mg/L didemnin B and nordidemnin B can be harvested fromfermentation of Tistrella mobilis using a simple medium for as short as3 days, improvement of the culture conditions may further increase theproduction of the didemnins. Because of their large molecular weight andhigh lipophilicity, purification of the didemnins is quite simple bychromatographic separation, for example, which in turn can provide thestarting material to semisynthesize dehydrodidemnin B. Furthermore,genetic engineering of the didemnin gene cluster provides novel didemnincompounds with improved biological activities.

Example 3 Other Didemnins Produced by Tistrella Mobilis

In particular aspects of the invention, there are didemnins other thandidemnin B and nordidemninB from Tistrella mobilis. As shown in FIGS. 6,7A, and 7B using UPLC-HRMS, there was detection of some other didemninssuch as didemnin A, nordidemnin A and didemnin N. In certain aspects,there are new didemnins with similar molecular weights but differentfrom all reported didemnins (see FIGS. 8A and 8B). They also havesimilar UV absorption patterns to didemnins.

Molecular Name Formula weight Reference Source Didemnin A C49H78N6O12943.177 Crampton, S. L. et al., Cancer Res., 44 (1984) 1796; M.B.Trididemnum sp. Hossain et al., Internat. J. Peptide Protein Res. 47(1996) 20-27 Didemnin B C57H89N7O15 1,112.35 Crampton. S. L. et al.,Cancer Res., 44 (1984) 1796; Trididemnum sp. Didemnin C C52H82N6O141,015.24 Rinehart, K. L. et al., Science, 212 (1981) 933; Trididemnumspp. Prolyldidenmin A C54H85N7O13 1,040.29 Schmidt, U. et al.,Tetrahedron Lett., 29, 4407-8 1988 didemnin D C77H118N14O23 1,607.84Rinehart KL Kishore V Bible KC Sakai R Sullins DW Li ChordataTrididemnum K-M J. Nat. Prod. 1988 51 1-21 solidum didemnin EC72H110N12O21 1,479.71 Rinehart KL Kishore V Bible KC Sakai R Sullins DWLi Chordata Trididemnum K-M J. Nat. Prod. 1988 51 1-21 solidum didemninG C58H89N7O16 1,140.36 K. L. Rinehart, 2nd Euroconf. Marine Nat. Prod.,1999 Trididemnum solidum Santiago d. Comp., isodidemnin-1 C57H89N7O151,112.35 Guyot M Davoust D Morel E C. R. Acad. Sci. P Ser. II ChordataTrididemnum 1987 305 681-686 cyanophorum nordidemnin B C56H87N7O151,098.33 McKee TC Ireland CM Lindquist N Fenical W Chordata TrididemnumTetrahedron Lett. 1989 30 3053-3056 solidum didemnin M C67H102N10O191,351.58 Sakai R Stroh JG Sullins DW Rinehart KL J. Am. Chem. ChordataTrididemnum Soc. 1995 117 3734-3748 solidum didemnin N C55H85N7O151,084.30 Sakai R Stroh JG Sullins DW Rinehart KL J. Am. Chem. ChordataTrididemnum Soc. 1995 117 3734-3748 solidum didemnin X C82H131N13O231,666.99 Sakai R Stroh JG Sullins DW Rinehart KL J. Am. Chem. ChordataTrididemnum Soc. 1995 117 3734-3748 solidum didemnin Y C87H139N15O251,795.12 Sakai R Stroh JG Sullins DW Rinehart KL J. Am. Chem. ChordataTrididemnum Soc. 1995 117 3734-3748 solidum nordidemnin N C54H83N7O151,070.27 Sakai R Stroh JG Sullins DW Rinehart KL J. Am. Chem. ChordataTrididemnum Soc. 1995 117 3734-3748 solidum epididemnin A1 C49H78N6O12943.177 Sakai R Stroh JG Sullins DW Rinehart KL J. Am. Chem. ChordataTrididemnum Soc. 1995 117 3734-3748 solidum acyclodidemnin A C49H80N6O13961.192 Sakai R Stroh JG Sullins DW Rinehart KL J. Am. Chem. ChordataTrididemnum Soc. 1995 117 3734-3748 solidum [tyr5]didemnin B C55H85N7O151,084.30 Aboumansour E Boulanger A Badre A Bonnard I Banaigs B ChordataTrididemnum Combaut G Francisco C Tetrahedron 1995 51 12591-12600cyanophorum [D-pro4]didemnin C57H89N7O15 1,112.35 Aboumansour EBoulanger A Badre A Bonnard I Banaigs B Chordata Trididemnum B Combaut GFrancisco C Tetrahedron 1995 51 12591-12600 cyanophorum didemnin HC67H102N10O19 1,351.58 Boulanger A Abou-Mansour E Badre A Banaigs BChordata Trididemnum Combaut G Francisco C Tetrahedron Lett. 1994 354345-4348 cyanophorum dehydrodidemnin C57H87N7O15 1,110.34 Rinehart KLLithgow-Bertelloni AM Ruffles GK PCT Chordata Aplidium B Int. Appl. 19910 0 albicans [Hysp2]didemnin C58H91N7O15 1,126.38 Banaigs B Mansour EABonnard I Boulanger A Francisco C Chordata Aplidium B Tetrahedron 199955 9559-9574 albicans, Chordata Trididemnum cyanophorum, ChordataTrididemnum solidum [Hap2]didemnin C54H83N7O15 1,070.27 Banaigs BMansour EA Bonnard I Boulanger A Francisco C Chordata Aplidium BTetrahedron 1999 55 9559-9574 albicans, Chordata Trididemnumcyanophorum, Chordata Trididemnum solidum

Example 4 Sequence Information

The following table provides annotation of the Tistrella mobilis genome(including plasmids 1-4 (P1-P4)).

Locus Gene Product Start End Strand Length P1.orf0001 dhbF Amino acidadenylation 1 3501 1 3501 P1.orf0002 HNH endonuclease domain-containingprotein 3566 4135 2 570 P1.orf0003 conserved hypothetical protein 42974632 1 336 P1.orf0004 conserved hypothetical protein 4675 4920 1 246P1.orf0005 conserved hypothetical protein 4910 5203 2 294 P1.orf0006Tetratricopeptide TPR_4 5622 7493 3 1872 P1.orf0007 hypothetical protein7712 7870 2 159 P1.orf0008 2-nitropropane dioxygenase NPD 10299 9328 −1972 P1.orf0009 cytochrome B561 10869 10303 −1 567 P1.orf0010 TPRrepeat-containing protein 11116 12300 1 1185 P1.orf0011 arcB ornithinecyclodeaminase 13363 12311 −2 1053 P1.orf0012 arcB arginase 14343 13360−1 984 P1.orf0013 hypothetical protein 15282 14491 −1 792 P1.orf0014transcriptional regulator 15452 15904 2 453 P1.orf0015 D-aminoacylase16007 17512 2 1506 P1.orf0016 cobW Cobalamin biosynthesis protein CobW17509 18474 1 966 P1.orf0017 kipR transcriptional regulator, IclR family18495 19313 3 819 P1.orf0018 transposase 19680 19988 3 309 P1.orf0019transposase, IS4 20131 20361 1 231 P1.orf0020 recF DNA replication andrepair protein recF 20617 22251 1 1635 P1.orf0021 yagA integrasecatalytic subunit 24237 23488 −1 750 P1.orf0022 dadA D-amino-aciddehydrogenase (DadA-like) 25991 24768 −3 1224 P1.orf0023 gsiA putativeABC transporter, ATP-binding protein 27732 26053 −1 1680 P1.orf0024putative ABC transporter, permease protein 28582 27737 −2 846 P1.orf0025putative ABC transporter, permease protein 29522 28803 −3 720 P1.orf0026binding-protein-dependent transport systems 28809 28582 −1 228 innermembrane component P1.orf0027 putative ABC transporter, periplasmicbinding 31211 29595 −3 1617 protein P1.orf0028 baeS two component sensorhistidine kinase, AdeS 32657 31479 −3 1179 P1.orf0029 putativetranscriptional regulator ycf27 33415 32654 −2 762 P1.orf0030 conservedhypothetical protein 33688 34317 1 630 P1.orf0031 conserved hypotheticalprotein 34400 35098 2 699 P1.orf0032 conserved hypothetical protein36007 35186 −2 822 P1.orf0033 hypothetical protein 36386 36081 −3 306P1.orf0034 Sulfatase-modifying factor 36553 37887 1 1335 P1.orf0035Meiotically up-regulated gene 158 protein 37915 38916 1 1002 P1.orf0036putative transcriptional regulator 39030 39296 3 267 P1.orf0037conserved hypothetical protein 39293 39718 2 426 P1.orf0038 conservedhypothetical protein 40110 39703 −1 408 P1.orf0039 conservedhypothetical protein 40969 40127 −2 843 P1.orf0040 hypothetical protein41084 40953 −3 132 P1.orf0041 conserved hypothetical protein 41113 416791 567 P1.orf0042 conserved hypothetical protein 41718 42971 3 1254P1.orf0043 yciE conserved hypothetical protein 42989 44128 2 1140P1.orf0044 TPR repeat 45084 44143 −1 942 P1.orf0045 conservedhypothetical protein 45365 45081 −3 285 P1.orf0046 aspartyl/asparaginylbeta-hydroxylase 46396 45362 −2 1035 P1.orf0047 asnB Asparagine synthase(glutamine-hydrolyzing) 48329 46389 −3 1941 P1.orf0048 hypotheticalprotein 48512 48342 −3 171 P1.orf0049 transcriptional regulator, AsnCfamily 49221 48742 −1 480 P1.orf0050 conserved hypothetical protein49373 50158 2 786 P1.orf0051 L-carnitine dehydratase/bile acid-inducible51309 50179 −1 1131 protein F P1.orf0052 exoD putative exopolysaccharidesynthesis protein 51530 52174 2 645 P1.orf0053 sensory box/GGDEF familyprotein 54320 52131 −3 2190 P1.orf0054 PEBP family protein 54541 56367 11827 P1.orf0055 hypothetical protein 56514 56780 3 267 P1.orf0056inositol-1-monophosphatase 56949 57809 3 861 P1.orf0057 hypotheticalprotein 58072 57815 −2 258 P1.orf0058 feaR AraC family transcriptionalregulator 58256 59380 2 1125 P1.orf0059 hypothetical protein 59582 599202 339 P1.orf0060 hypothetical protein 60233 59958 −3 276 P1.orf0061conserved hypothetical protein 60341 60535 2 195 P1.orf0062 hypotheticalprotein 61819 60983 −2 837 P1.orf0063 conserved hypothetical protein62520 61816 −1 705 P1.orf0064 hypothetical protein 63257 62658 −3 600P1.orf0065 hypothetical protein 63988 63254 −2 735 P1.orf0066 conservedhypothetical protein 65137 64061 −2 1077 P1.orf0067 conservedhypothetical protein 66168 65134 −1 1035 P1.orf0068 vgrG protein 6892466195 −3 2730 P1.orf0069 conserved hypothetical protein 69570 69058 −1513 P1.orf0070 conserved hypothetical protein 71219 69567 −3 1653P1.orf0071 ABC transporter, permease protein 72438 71212 −1 1227P1.orf0072 macB Macrolide export ATP-binding/permease 73142 72438 −3 705protein P1.orf0073 ppkA-related protein 75115 73139 −2 1977 P1.orf0074serine/threonine kinase 76542 75115 −1 1428 P1.orf0075 prpC Ser/Thrprotein phosphatase 77305 76535 −2 771 P1.orf0076 conserved hypotheticalprotein 78018 77302 −1 717 P1.orf0077 conserved hypothetical protein81725 78000 −3 3726 P1.orf0078 ytxE conserved hypothetical protein 8326381725 −2 1539 P1.orf0079 conserved hypothetical protein 84610 83267 −21344 P1.orf0080 type VI secretion lipoprotein, VC_A0113 family 8517884669 −3 510 P1.orf0081 FHA domain-containing protein 86617 85211 −21407 P1.orf0082 type VI secretion ATPase, ClpV1 family 89264 86661 −32604 P1.orf0083 type VI secretion system protein ImpH 90365 89334 −31032 P1.orf0084 type VI secretion system protein ImpG 92216 90375 −31842 P1.orf0085 type VI secretion system protein ImpF 92731 92213 −2 519P1.orf0086 type VI secretion system protein ImpE 93597 92728 −1 870P1.orf0087 type VI secretion system protein ImpC 95063 93663 −3 1401P1.orf0088 type VI secretion system protein ImpC 96576 95080 −1 1497P1.orf0089 type VI secretion protein 97115 96582 −3 534 P1.orf0090impA-related N-terminal protein 98317 97175 −2 1143 P1.orf0091 peptidaseS1 and S6, chymotrypsin/Hap 99083 98634 −3 450 P1.orf0092 hcp1 conservedhypothetical protein 99418 99891 1 474 P1.orf0093 hypothetical protein100070 100681 2 612 P1.orf0094 hypothetical protein 100695 101285 3 591P1.orf0095 VgrG protein 101290 103272 1 1983 P1.orf0096 hypotheticalprotein 103322 104083 2 762 P1.orf0097 conserved hypothetical protein104130 104543 3 414 P1.orf0098 rhsC putative rhs-related transmembraneprotein 104548 109017 1 4470 P1.orf0099 conserved hypothetical protein109034 109873 2 840 P1.orf0100 conserved hypothetical protein 109983110342 3 360 P1.orf0101 Ribosomal protein L7/L12 C-terminal domain.110467 110853 1 387 P1.orf0102 hypothetical protein 110962 111171 1 210P1.orf0103 hypothetical protein 111187 111699 1 513 P1.orf0104 conservedhypothetical protein 111745 112572 1 828 P1.orf0105 conservedhypothetical protein 112700 113104 2 405 P1.orf0106 conservedhypothetical protein 113104 113838 1 735 P1.orf0107 treYmalto-oligosyltrehalose synthase 113999 116740 2 2742 P1.orf0108lipolytic enzyme 117759 116767 −1 993 P1.orf0109 paiB transcriptionalrepressor of sporulation and 117861 118490 3 630 degradative enzymeproduction P1.orf0110 Metallophosphoesterase 119313 118522 −1 792P1.orf0111 ywfA putative transporter protein 119527 120717 1 1191P1.orf0112 ybfI AraC family transcriptional regulator 120779 121609 2831 P1.orf0113 conserved hypothetical protein 121817 121620 −3 198P1.orf0114 conserved hypothetical protein 122207 121902 −3 306P1.orf0115 putative addiction module antidote protein, 122540 122764 2225 CopG/Arc/MetJ family P1.orf0116 plasmid stabilization system protein122765 123076 2 312 P1.orf0117 conserved hypothetical protein 123161124144 2 984 P1.orf0118 ywjA ABC transporter, nucleotide binding/ATPase125820 124108 −1 1713 protein P1.orf0119 fhuB transport system permeaseprotein 126404 125895 −3 510 P1.orf0120 fhuB transport system permeaseprotein 127864 126395 −2 1470 P1.orf0121 fhuDIron(3+)-hydroxamate-binding protein fhuD 128711 127857 −3 855P1.orf0122 fhuC iron-hydroxamate transporter ATP-binding 129528 128731−1 798 subunit P1.orf0123 fhuA ferrichrome receptor precursor protein131773 129539 −2 2235 P1.orf0124 msmR transcriptional regulator, AraCfamily 132821 131928 −3 894 P1.orf0125 Regulator of cell morphogenesisand NO 133622 132918 −3 705 signaling P1.orf0126 GCN5-relatedN-acetyltransferase 134591 133734 −3 858 P1.orf0127 appF putativedipeptide ABC transporter, ATP- 135640 134654 −2 987 binding proteinP1.orf0128 Oligopeptide/dipeptide transporter domain 136449 135637 −1813 family protein P1.orf0129 ddpC ABC transporter permease protein 1137342 136446 −3 897 P1.orf0130 ddpB binding-protein dependent transportsystem 138361 137339 −2 1023 inner membrane protein P1.orf0131 hbpAextracellular solute-binding protein family 5 140053 138434 −2 1620P1.orf0132 soxC DszC-like desulfurization enzyme 141285 140104 −1 1182P1.orf0133 soxA flavin-dependent oxidoreductase 142712 141282 −3 1431P1.orf0134 hcaR transcriptional regulator, LysR family 144315 143398 −1918 P1.orf0135 short-chain dehydrogenase/reductase SDR 144452 145222 2771 P1.orf0136 catD carboxylesterase 146146 145223 −2 924 P1.orf0137conserved hypothetical protein 146388 147218 3 831 P1.orf0138 acyl-CoAdehydrogenase 147267 148451 3 1185 P1.orf0139 hypothetical protein146396 146217 −3 180 P1.orf0140 acyl-CoA dehydrogenase family protein148483 149682 1 1200 P1.orf0141 hypothetical protein 150210 149764 −1447 P1.orf0142 parA Cobyrinic acid ac-diamide synthase 151061 150207 −3855 P1.orf0143 hypothetical protein 151289 151116 −3 174 P1.orf0144transcriptional regulator, CopG family 151688 151413 −3 276 P1.orf0145mtnC 2,3-diketo-5-methylthio-1-phosphopentane 152572 151757 −2 816phosphatase P1.orf0146 mtnD acireductone dioxygenase ARD 153107 152562−3 546 P1.orf0147 mtnB methylthioribulose-1-phosphate dehydratase 153733153104 −2 630 P1.orf0148 replication protein A 156147 157436 3 1290P1.orf0149 hypothetical protein 156098 155670 −3 429 P1.orf0150hypothetical protein 158775 159935 3 1161 P1.orf0151 conservedhypothetical protein 159979 166170 1 6192 P1.orf0152 dctB two-componentsensor histidine kinase protein 166326 168158 3 1833 P1.orf0153 dctD twocomponent, sigma54 specific, 168155 169513 2 1359 transcriptionalregulator, Fis family P1.orf0154 TRAP transporter solute receptor, TAXIfamily 169676 170662 2 987 protein P1.orf0155 TRAP transporter, 4TM/12TMfusion protein 170765 172939 2 2175 P1.orf0156 3-methylcrotonoyl-CoAcarboxylase beta 174655 173048 −2 1608 subunit P1.orf0157 Pcca putativeacyl-CoA carboxylase, Biotin/lipoyl 175185 174652 −1 534 carrier domainP1.orf0158 pycA acetyl/propionyl CoA carboxylase alpha 176739 175264 −11476 subunit P1.orf0159 citE Citryl-CoA lyase 177666 176746 −1 921P1.orf0160 dehydratase 178133 177663 −3 471 P1.orf0161 short-chaindehydrogenase/reductase SDR 178304 179137 2 834 P1.orf0162 IVDIsovaleryl-CoA dehydrogenase 180424 179222 −2 1203 P1.orf0163 IivF ABCtransporter related protein 181236 180448 −1 789 P1.orf0164 putativebranched-chain amino acid transport 182524 181244 −2 1281 systemsubstrate-binding protein P1.orf0165 braE inner-membrane translocator183668 182595 −3 1074 P1.orf0166 braD inner-membrane translocator 184561183674 −2 888 P1.orf0167 AMP-dependent synthetase and ligase 186533184563 −3 1971 P1.orf0168 braF ABC transporter related protein 187335186523 −1 813 P1.orf0169 fadR TetR family transcriptional regulator188519 187803 −3 717 P1.orf0170 hypothetical protein 188784 189266 3 483P1.orf0171 tetR Tetracycline repressor protein class H 189987 189301 −1687 P1.orf0172 eutQ Ethanolamine utilization protein eutQ 190133 1905132 381 P1.orf0173 ordL FAD dependent oxidoreductase 190510 191829 1 1320P1.orf0174 yiiZ deoxycytidine triphosphate (dCTP) deaminase 191903192943 2 1041 P1.orf0175 TRAP-type C4-dicarboxylate transport system192995 193501 2 507 small permease component P1.orf0176 siaT putativeDctM (C4-dicarboxylate permease, 193503 194792 3 1290 large subunit)P1.orf0177 hypothetical protein 195002 195325 2 324 P1.orf0178 conservedhypothetical protein 195425 195892 2 468 P1.orf0179 TetR familytranscriptional regulator 196517 195846 −3 672 P1.orf0180 yesFoxidoreductase yesF 196619 197461 2 843 P1.orf0181 calB aldehydedehydrogenase 198848 197430 −3 1419 P1.orf0182 TetR familytranscriptional regulator 199683 199045 −1 639 P1.orf0183 conservedhypothetical protein 199899 200906 3 1008 P1.orf0184 putative pyridoxine5′-phosphate oxidase 201927 201238 −1 690 P1.orf0185 LysR familytranscriptional regulator 202044 202982 3 939 P1.orf0186 conservedhypothetical protein 204005 202992 −3 1014 P1.orf0187 conservedhypothetical protein 204591 203998 −1 594 P1.orf0188 cydB cytochrome dubiquinol oxidase, subunit II 206024 204870 −3 1155 P1.orf0189 cydAcytochrome D ubiquinol oxidase, subunit I 207644 206031 −3 1614P1.orf0190 cydC transport ATP-binding protein CYDC 209417 207732 −3 1686P1.orf0191 cydD ABC transporter, CydDC cysteine exporter 211018 209444−2 1575 (CydDC-E) family, permease/ATP-binding protein CydD P1.orf0192hypothetical protein 211079 211204 2 126 P1.orf0193 dcrAmethyl-accepting chemotaxis protein 211329 213422 3 2094 P1.orf0194putative araC-like transcription regulator 214364 213435 −3 930P1.orf0195 yusZ short-chain dehydrogenase/reductase SDR 214465 215220 1756 P1.orf0196 ubiE Methyltransferase type 11 216154 215240 −2 915P1.orf0197 major facilitator transporter 217467 216211 −1 1257P1.orf0198 fyuA TonB-dependent receptor 219740 217473 −3 2268 P1.orf0199pchR regulatory protein Pchr 220674 219814 −1 861 P1.orf0200hypothetical protein 221164 220736 −2 429 P1.orf0201 hypotheticalprotein 221470 221198 −2 273 P1.orf0202 transposase, IS4 222251 221904−3 348 P1.orf0203 PilT domain-containing protein 222699 222268 −1 432P1.orf0204 conserved hypothetical protein 222905 222696 −3 210P1.orf0205 conserved hypothetical protein 223604 223110 −3 495P1.orf0206 dhkJ multi-sensor hybrid histidine kinase 228426 223648 −14779 P1.orf0207 ybaR sulphate transporter 229028 230536 2 1509P1.orf0208 putative addiction module antidote protein, 230716 230997 1282 CopG/Arc/MetJ family P1.orf0209 hypothetical protein 231751 231569−2 183 P1.orf0210 hypothetical protein 232640 232413 −3 228 P1.orf0211malQ glycoside hydrolase family protein 234541 232637 −2 1905 P1.orf0212glgX glycogen debranching enzyme GlgX 238557 234628 −1 3930 P1.orf0213glycogen branching enzyme 240732 238567 −1 2166 P1.orf0214 treStrehalose synthase 244194 240808 −1 3387 P1.orf0215 aam1 alpha amylasecatalytic region 247202 244200 −3 3003 P1.orf0216 hypothetical protein247741 247244 −2 498 P1.orf0217 hypothetical protein 247736 247966 2 231P1.orf0218 cyclase/dehydrase 248034 248840 3 807 P1.orf0219 otsBtrehalose-phosphatase 248837 249631 2 795 P1.orf0220 otsAalpha,alpha-trehalose-phosphate synthase 249734 251275 2 1542(UDP-forming) P1.orf0221 TRAP dicarboxylate transporter, DctM subunit252686 251367 −3 1320 P1.orf0222 TRAP dicarboxylate transporter, DctQsubunit 253240 252683 −2 558 P1.orf0223 yiaO TRAP dicarboxylate familytransporter, DctP 254661 253585 −1 1077 subunit P1.orf0224 mvaAhydroxymethylglutaryl-CoA reductase, 256027 254723 −2 1305 degradativeP1.orf0225 ydcR GntR family transcriptional regulator 256219 257616 11398 P1.orf0226 AraC family transcriptional regulator 258898 257852 −21047 P1.orf0227 Extracellular ligand-binding receptor 259239 260420 31182 P1.orf0228 IivH inner-membrane translocator 260507 261364 2 858P1.orf0229 IivM inner-membrane translocator 261376 262317 1 942P1.orf0230 braF ABC transporter related protein 262314 263063 3 750P1.orf0231 IivF ABC transporter related protein 263060 263761 2 702P1.orf0232 acetyl-CoA acyltransferase 263771 265003 2 1233 P1.orf0233lcfB Acyl-CoA synthetase (AMP-forming)/AMP-acid 265013 266563 2 1551ligase II P1.orf0234 fadD AMP-binding enzyme 266573 268081 2 1509P1.orf0235 SCP2 lipid-transfer protein 268098 269279 3 1182 P1.orf02363-oxoacyl-[acyl-carrier protein] reductase 269276 270085 2 810P1.orf0237 conserved hypothetical protein 270216 270575 3 360 P1.orf0238F440524_38 putative alcohol dehydrogenase 270819 270637 −1 183P1.orf0239 conserved hypothetical protein 270867 271124 3 258 P1.orf0240addiction module toxin, RelE/StbE family 271121 271423 2 303 P1.orf0241putative Alcohol dehydrogenase, (ADH) 271793 271398 −3 396 P1.orf0242hypothetical protein 271927 271802 −2 126 P1.orf0243 dhkJ multi-sensorhybrid histidine kinase 272012 276670 2 4659 P1.orf0244 adhA Alcoholdehydrogenase GroES domain protein 277765 276710 −2 1056 P1.orf0245 yjfFinner-membrane translocator 278908 277865 −2 1044 P1.orf0246 ytfT sugarABC transporter permease protein 279921 278908 −1 1014 P1.orf0247 ytfRsugar ABC transporter ATP-binding protein 281503 279995 −2 1509P1.orf0248 ytfQ periplasmic binding protein/LacI transcriptional 282545281586 −3 960 regulator P1.orf0249 Galm aldose 1-epimerase 283693 282674−2 1020 P1.orf0250 yvrE Smp-30/Cgr1 family protein 284592 283690 −1 903P1.orf0251 aldH dehydrogenase 285562 284582 −2 981 P1.orf0252 aldHNAD-dependent aldehyde dehydrogenase 286131 285559 −1 573 P1.orf0253 galputative d-galactose 1-dehydrogenase protein 287110 286163 −2 948P1.orf0254 ilvD dihydroxy-acid dehydratase 288839 287097 −3 1743P1.orf0255 fumarylacetoacetate (FAA) hydrolase 289859 288858 −3 1002P1.orf0256 xylH inner membrane permease of D-xylose ABC 291069 289864 −11206 transporter P1.orf0257 araG ABC transporter related protein 292603291041 −2 1563 P1.orf0258 chvE putative sugar uptake ABC transporter293736 292666 −1 1071 periplasmic solute-binding protein precursorP1.orf0259 gbpR transcriptional regulator protein, LysR family 293961294938 3 978 (possibly activator of the expression of chvE protein)P1.orf0260 conserved hypothetical protein 295016 295489 2 474 P1.orf0261LysR family transcriptional regulator 296609 297565 2 957 P1.orf0262Alcohol dehydrogenase zinc-binding domain 296536 295508 −2 1029 proteinP1.orf0263 conserved hypothetical protein 297888 297619 −1 270P1.orf0264 conserved hypothetical protein 298075 297848 −2 228P1.orf0265 TetR family transcriptional regulator 298803 298174 −1 630P1.orf0266 mdmC O-methyltransferase mdmC 298931 299602 2 672 P1.orf0267major facilitator superfamily transporter 299655 300839 3 1185P1.orf0268 conserved hypothetical protein 300897 301634 3 738 P1.orf0269acrF acriflavin resistance protein 305336 302289 −3 3048 P1.orf0270efflux transporter, RND family, MFP subunit 306451 305336 −2 1116P1.orf0271 lmrA transcriptional regulator 306540 307115 3 576 P1.orf0272vdlC short chain dehydrogenase 307162 307977 1 816 P1.orf0273 TRR1FAD-dependent pyridine nucleotide-disulphide 308915 307995 −3 921oxidoreductase P1.orf0274 ywnA transcriptional regulator, BadM/Rrf2family 309078 309569 3 492 P1.orf0275 putative effector of mureinhydrolase 310317 309583 −1 735 P1.orf0276 hypothetical protein 310717310310 −2 408 P1.orf0277 pmrA major facilitator transporter 311875310790 −2 1086 P1.orf0278 folE GTP cyclohydrolase I 312630 312004 −1 627P1.orf0279 conserved hypothetical protein 313395 312655 −1 741P1.orf0280 conserved hypothetical protein 313853 314236 2 384 P1.orf0281conserved hypothetical protein 314242 315081 1 840 P1.orf0282arylmalonate decarboxylase 315223 315966 1 744 P1.orf0283 ydhCtranscriptional regulator, GntR family 315981 316685 3 705 P1.orf0284putative acyl-CoA transferase/carnitine 316686 317882 3 1197 dehydrataseP1.orf0285 yngG Hydroxymethylglutaryl-CoA lyase 317894 318838 2 945P1.orf0286 gbas conserved hypothetical protein 318835 319149 1 315P1.orf0287 isochorismatase hydrolase 319182 319835 3 654 P1.orf0288 mauRLysR family transcriptional regulator 319929 320825 3 897 P1.orf0289yajO putative oxidoreductase 321960 320899 −1 1062 P1.orf0290 conservedhypothetical protein 322495 322088 −2 408 P1.orf0291 WD40-like repeat323353 322676 −2 678 P1.orf0292 conserved hypothetical protein 324680323472 −3 1209 P1.orf0293 hypothetical protein 325200 325018 −1 183P1.orf0294 braC branched chain amino acid ABC transporter 325402 3265411 1140 periplasmic ligand-binding protein P1.orf0295 braD ABC branchedchain amino acid family 326563 327480 1 918 transporter, inner membranesubunit P1.orf0296 braE High-affinity branched-chain amino acid 327562328494 1 933 transport system permease protein braE P1.orf0297 braFbranched-chain amino acid ABC transporter 328491 329318 3 828 ATPaseP1.orf0298 braG High-affinity branched-chain amino acid 329323 330018 1696 transport ATP-binding protein braG P1.orf0299 putative hydrolase330037 330909 1 873 P1.orf0300 yfdE L-carnitine dehydratase/bileacid-inducible 331052 332284 2 1233 protein F P1.orf0301 hypotheticalprotein 332281 333126 1 846 P1.orf0302 namA putative FMN oxidoreductase333123 334307 3 1185 P1.orf0303 MscS mechanosensitive ion channel 335475334321 −1 1155 P1.orf0304 yagR aldehyde oxidase and xanthine 337817335520 −3 2298 dehydrogenase molybdopterin binding P1.orf0305 yagSmolybdopterin dehydrogenase FAD-binding 338830 337814 −2 1017 P1.orf0306yagT oxidoreductase 339306 338830 −1 477 P1.orf0307 hypothetical protein339700 339341 −2 360 P1.orf0308 conserved hypothetical protein 339834340160 3 327 P1.orf0309 pyridoxal phosphate biosynthetic protein PdxJ340527 340315 −1 213 P1.orf0310 cold-shock DNA-binding domain-containing340993 340799 −2 195 protein P1.orf0311 DNA ligase D 343739 341301 −32439 P1.orf0312 Ku domain-containing protein 344647 343745 −2 903P1.orf0313 conserved hypothetical protein 344803 345087 1 285 P1.orf0314mntH NADPH-dependent FMN reductase 345091 345735 1 645 P1.orf0315 yhdFShort-chain dehydrogenase/reductase SDR 346645 345794 −2 852 P1.orf0316hypothetical protein 346993 347313 1 321 P1.orf0317 cmoB putativeSAM-dependent methyltransferase 348091 347378 −2 714 P1.orf0318topoisomerase IB 349134 348118 −1 1017 P1.orf0319 Uxs1 NAD-dependentepimerase/dehydratase 350294 349191 −3 1104 P1.orf0320 conservedhypothetical protein 351465 350305 −1 1161 P1.orf0321 conservedhypothetical protein 352556 351462 −3 1095 P1.orf0322 conservedhypothetical protein 353707 352553 −2 1155 P1.orf0323 glycosyltransferase, group 1 354876 353704 −1 1173 P1.orf0324 radical SAMdomain-containing protein 356162 354864 −3 1299 P1.orf0325 rfbENAD-dependent epimerase/dehydratase 358207 356159 −2 2049 P1.orf0326NAD-dependent epimerase/dehydratase 359322 358204 −1 1119 P1.orf0327rpoE ECF subfamily RNA polymerase sigma-24 360114 359527 −1 588 factorP1.orf0328 conserved hypothetical protein 361269 360760 −1 510P1.orf0329 cc4 cytochrome c, class I 362430 361306 −1 1125 P1.orf0330Cytochrome c oxidase caa3-type, assembly 363092 362427 −3 666 factorCtaG-related protein P1.orf0331 transmembrane prediction 363451 363089−2 363 P1.orf0332 ctaD cytochrome c oxidase, subunit I 365985 363466 −12520 P1.orf0333 ctaC cytochrome c oxidase, subunit II 367010 365982 −31029 P1.orf0334 gcd glucose dehydrogenase 369412 367016 −2 2397P1.orf0335 hypothetical protein 369479 369637 2 159 P1.orf0336 conservedhypothetical protein 370254 370700 3 447 P1.orf0337 conservedhypothetical protein 371128 371805 1 678 P1.orf0338 conservedhypothetical protein 373405 373043 −2 363 P1.orf0339 prevent-host-deathfamily protein 373714 373454 −2 261 P1.orf0340 gabD AldehydeDehydrogenase 375058 373802 −2 1257 P1.orf0341 gsiA oligopeptide ABCsuperfamily ATP binding 376993 375308 −2 1686 cassette transporter, ABCprotein P1.orf0342 Putative peptide transport system permease 377814376990 −1 825 protein P1.orf0343 binding-protein-dependent transportsystems 378769 377825 −2 945 inner membrane component P1.orf0344acyl-coenzyme A: 6-aminopenicillanic-acid- 379943 378822 −3 1122acyltransferase precursor P1.orf0345 beta-alanine-pyruvate transaminase381420 380041 −1 1380 P1.orf0346 yjjM GntR family transcriptionalregulator 382417 381500 −2 918 P1.orf0347 bam Indoleacetamide hydrolase382501 383991 1 1491 P1.orf0348 poly(aspartic acid) hydrolase 384006384872 3 867 P1.orf0349 ABC superfamily ATP binding cassette 385008386591 3 1584 transporter substrate-binding protein P1.orf0350transposase, mutator type 387405 387115 −1 291 P1.orf0351 transposase,mutator type 388310 387405 −3 906 P1.orf0352 conserved hypotheticalprotein 388987 389457 1 471 P1.orf0353 conserved hypothetical protein390709 389783 −2 927 P1.orf0354 conserved hypothetical protein 391494390706 −1 789 P1.orf0355 plasmid maintenance system antidote protein,392861 393004 2 144 XRE family P1.orf0356 type II restriction enzyme,methylase subunit 393001 393192 1 192 P1.orf0357 hsdR type Irestriction-modification system 393202 396231 1 3030 restriction subunitP1.orf0358 putative restriction modification system 396266 397510 2 1245specificity subunit P1.orf0359 prrC anticodon nuclease 397507 398736 11230 P1.orf0360 type I restriction-modification system, M 398733 4003553 1623 subunit P1.orf0361 integrase, catalytic region 400892 401353 2462 P1.orf0362 xerC phage integrase family protein 402015 402977 3 963P1.orf0363 conserved hypothetical protein 403509 402967 −1 543P1.orf0364 conserved hypothetical protein 403679 405373 2 1695P1.orf0365 conserved hypothetical protein 405379 407313 1 1935P1.orf0366 conserved hypothetical protein 407310 408947 3 1638P1.orf0367 Cold-shock DNA-binding domain protein 408944 411208 2 2265P1.orf0368 conserved hypothetical protein 411205 412182 1 978 P1.orf0369conserved hypothetical protein 416011 412664 −2 3348 P1.orf0370 al2CRISPR-associated protein 416875 418866 1 1992 P1.orf0371CRISPR-associated protein 418867 419184 1 318 P1.orf0372 selenide, waterdikinase 419865 420392 3 528 P1.orf0373 ybfL transposase, is4 family420664 421710 1 1047 P1.orf0374 parA partition protein ParA 422509423213 1 705 P1.orf0375 hypothetical protein 423411 423569 3 159P1.orf0376 hypothetical protein 423954 424079 3 126 P1.orf0377 gluAF213463_1 cellobiase CelA precursor 424429 426300 1 1872 P1.orf0378maleate cis-trans isomerase protein 427582 426830 −2 753 P1.orf0379 icaRputative TetR family transcriptional regulator 427725 428483 3 759P1.orf0380 conserved hypothetical protein 428775 428515 −1 261P1.orf0381 glyoxalase/bleomycin resistance 429479 428775 −3 705protein/dioxygenase P1.orf0382 conserved hypothetical protein 430769429501 −3 1269 P1.orf0383 conserved hypothetical protein 431298 430777−1 522 P1.orf0384 vioD putative monooxygenase, FAD/NAD(P)- 432452 431295−3 1158 binding domain P1.orf0385 dhbE AMP-dependent synthetase andligase 434172 432484 −1 1689 P1.orf0386 icaR putative transcriptionalregulator 434976 434275 −1 702 P1.orf0387 yiaO Bacterial extracellularsolute-binding protein, 435118 436176 1 1059 family 7 P1.orf0388conserved hypothetical protein 436161 436919 3 759 P1.orf0389 siaT TRAPtransporter, DctM-like membrane 436879 438048 1 1170 protein P1.orf0390putative Aminoglycoside phosphotransferase 439093 438071 −2 1023P1.orf0391 tam trans-aconitate methyltransferase 439218 439982 3 765P1.orf0392 gst glutathione S-transferase protein 440001 440609 3 609P1.orf0393 gloA lactoylglutathione lyase 441036 440632 −1 405 P1.orf0394conserved hypothetical protein 441639 441223 −1 417 P1.orf0395 acrR TetRfamily transcriptional regulator 441721 442362 1 642 P1.orf0396 ifcAfumarate reductase 443799 442378 −1 1422 P1.orf0397 namA NADH: flavinoxidoreductase/NADH oxidase 443958 444935 3 978 P1.orf0398 yvoAtranscriptional regulator 445026 445901 3 876 P1.orf0399 branched-chainamino acid transport system 446000 447223 2 1224 substrate-bindingprotein P1.orf0400 braD branched-chain amino acid ABC transporter 447325448167 1 843 permease protein P1.orf0401 amino acid ABC transporterpermease protein 448167 449150 3 984 P1.orf0402 lptB ABC transporterATP-binding protein 449150 449893 2 744 P1.orf0403 IivF ABC transporterATP-binding protein 449890 450609 1 720 P1.orf0404 MmgE/PrpD familyprotein 450622 452040 1 1419 P1.orf0405 prpB isocitrate lyase familyprotein 452042 452920 2 879 P1.orf0406 tautomerase 452966 453457 2 492P1.orf0407 ifcA putative fumarate reductase/succinate 453463 454821 11359 dehydrogenase flavoprotein P1.orf0408 hyuE hydantoin racemase454841 455599 2 759 P1.orf0409 hyuE hydantoin racemase 455614 456303 1690 P1.orf0410 lrg1 MmgE/PrpD family protein 456300 457646 3 1347P1.orf0411 hypothetical protein 457718 457864 2 147 P1.orf0412hypothetical protein 457895 458059 2 165 P1.orf0413 conservedhypothetical protein 458269 459588 1 1320 P1.orf0414 putative amidase461101 459668 −2 1434 P1.orf0415 braG branched chain amino acid ABCtransporter 461821 461129 −2 693 ATP-binding protein P1.orf0416 braFputative ABC transport system, ATP-bidning 462606 461815 −1 792 proteinP1.orf0417 IivM putative ABC transport system, membrane 463601 462603 −3999 protein P1.orf0418 braD inner-membrane translocator 464465 463605 −3861 P1.orf0419 branched-chain amino acid ABC transporter, 465776 464556−3 1221 periplasmic amino acid-binding protein P1.orf0420 nanRregulatory protein GntR HTH 466752 465961 −1 792 P1.orf0421 hypotheticalprotein 467173 467316 1 144 P1.orf0422 hypothetical protein 467699467899 2 201 P1.orf0423 hypothetical protein 467955 468107 3 153P1.orf0424 hypothetical protein 468152 468298 2 147 P1.orf0425 conservedhypothetical protein 468358 469305 1 948 P1.orf0426 algI membrane boundO-acyl transferase, MBOAT 469322 470677 2 1356 P1.orf0427 hypotheticalprotein 471709 470678 −2 1032 P1.orf0428 hypothetical protein 472081471875 −2 207 P1.orf0429 hypothetical protein 472147 472290 1 144P1.orf0430 hypothetical protein 473393 472362 −3 1032 P1.orf0431hypothetical protein 473501 473692 2 192 P1.orf0432 conservedhypothetical protein 473712 473978 3 267 P1.orf0433 cinAcompetence/damage-inducible protein CinA 473929 474507 1 579 P1.orf0434cat1 putative acetyl-CoA hydrolase/transferase 474600 476111 3 1512family protein; putative succinyl- CoA: coenzyme A transferaseP1.orf0435 alkJ putative choline dehydrogenase lipoprotein 476491 4781161 1626 oxidoreductase P1.orf0436 siaP TRAP dicarboxylate transporter-DctP subunit 478397 479503 2 1107 P1.orf0437 tripartite ATP-independentperiplasmic 479565 480071 3 507 transporter DctQ P1.orf0438 TRAPdicarboxylate transporter, DctM subunit 480068 481345 2 1278 P1.orf0439yxeQ MmgE/PrpD family protein 481377 482729 3 1353 P1.orf0440 maoCconserved hypothetical protein 482756 483256 2 501 P1.orf0441 mmgCButyryl-CoA dehydrogenase 483253 484428 1 1176 P1.orf0442 maoC conservedhypothetical protein 484439 484921 2 483 P1.orf0443 yfdE L-carnitinedehydratase/bile acid-inducible 484918 486153 1 1236 protein FP1.orf0444 Citryl-CoA lyase 486150 487073 3 924 P1.orf0445 kdgRtranscriptional regulator, IclR family 487146 487898 3 753 P1.orf0446major facilitator transporter 487911 489089 3 1179 P1.orf0447 Ech1enoyl-CoA hydratase 489947 489102 −3 846 P1.orf0448 hypothetical protein490444 490049 −2 396 P1.orf0449 hypothetical protein 490967 490515 −3453 P1.orf0450 hypothetical protein 491546 491067 −3 480 P1.orf0451 gst3Glutathione S-transferase domain protein 492356 491676 −3 681 P1.orf0452mmgC acyl-CoA dehydrogenase 493553 492408 −3 1146 P1.orf0453Carbamoyl-phosphate synthase L chain ATP- 495592 493598 −2 1995 bindingP1.orf0454 propionyl-CoA carboxylase 497235 495673 −1 1563 P1.orf0455hypothetical protein 497323 497616 1 294 P1.orf0456 mrcA glycosyltransferase family protein 498482 497871 −3 612 P1.orf0457transcriptional regulator 499199 498924 −3 276 P1.orf0458 hypotheticalprotein 499669 499484 −2 186 P1.orf0459 frpC bacteriocin 501632 499881−3 1752 P1.orf0460 hypothetical protein 501804 501619 −1 186 P1.orf0461hypothetical protein 502315 501893 −2 423 P1.orf0462 putative ENOYL-COAHYDRATASE 502566 503336 3 771 P1.orf0463 araC transcriptional regulator,AraC family 503355 504209 3 855 P1.orf0464 lcfAlong-chain-fatty-acid--CoA ligase 504260 506095 2 1836 P1.orf0465 rhlGshort-chain dehydrogenase/reductase SDR 506873 506106 −3 768 P1.orf0466pleC non-motile and phage-resistance protein 508451 507000 −3 1452P1.orf0467 membrane protein 508592 508915 2 324 P1.orf0468 conservedhypothetical protein 510548 508890 −3 1659 P1.orf0469 hypotheticalprotein 510922 510668 −2 255 P1.orf0470 luxQ sensor histidine kinase511186 514770 1 3585 P1.orf0471 two component transcriptional regulator514767 515732 3 966 P1.orf0472 conserved hypothetical protein 515942516124 2 183 P1.orf0473 hypothetical protein 516354 516656 3 303P1.orf0474 ynaD GCN5-related N-acetyltransferase 516833 517366 2 534P1.orf0475 conserved hypothetical protein 521851 517388 −2 4464P1.orf0476 conserved hypothetical protein 529416 521851 −1 7566P1.orf0477 hypothetical protein 530907 529468 −1 1440 P1.orf0478hemagglutinin protein 534719 530907 −3 3813 P1.orf0479 conservedhypothetical protein 537877 534716 −2 3162 P1.orf0480 conservedhypothetical protein 539720 537975 −3 1746 P1.orf0481 major facilitatortransporter 541177 539945 −2 1233 P1.orf0482 arsC ArsR familytranscriptional regulator/protein 541322 542170 2 849 tyrosinephosphatase P1.orf0483 gap3 glyceraldehyde-3-phosphate dehydrogenase,542175 543197 3 1023 type I P1.orf0484 major facilitator transporter543191 544441 2 1251 P1.orf0485 beta-lactamase 544538 545719 2 1182P1.orf0486 Cephalosporin hydroxylase 546109 546777 1 669 P1.orf0487macrocin-O-methyltransferase 546777 547604 3 828 P1.orf0488 conservedhypothetical protein 547960 548265 1 306 P1.orf0489 hypothetical protein548277 549212 3 936 P1.orf0490 O-linked N-acetylglucosamine transferase549206 550879 2 1674 P1.orf0491 hypothetical protein 550831 551082 1 252P1.orf0492 dehydratase, MaoC family protein 551432 551587 2 156P1.orf0493 citE HpcH/HpaI aldolase/citrate lyase family 551584 552501 1918 P1.orf0494 conserved hypothetical protein 552513 553436 3 924P1.orf0495 IivM ABC transporter permease protein 555566 554586 −3 981P1.orf0496 IivH inner-membrane translocator 556600 555731 −2 870P1.orf0497 IivF ABC transporter related protein 557327 556614 −3 714P1.orf0498 braF ABC transporter related protein 558115 557324 −2 792P1.orf0499 IivK putative leucine/isoleucine/valine-binding 559395 558163−1 1233 protein precursor P1.orf0500 baiB AMP-dependent synthetase andligase 559600 561189 1 1590 P1.orf0501 mfeB MaoC-like dehydratase 561274562128 1 855 P1.orf0502 short-chain dehydrogenase 562184 563104 2 921P1.orf0503 paaG crotonase 563120 563935 2 816 P1.orf0504 badR putativeMarR family transcriptional regulator 564533 563985 −3 549 P1.orf0505fadH short-chain dehydrogenase/reductase SDR 565439 564561 −3 879P1.orf0506 ymfI short-chain dehydrogenase/reductase SDR 566278 565538 −2741 P1.orf0507 putative acyl-CoA hydratase 567137 566289 −3 849P1.orf0508 yxbG 2,5-dichloro-2,5-cyclohexadiene-1,4-diol 567916 567161−2 756 dehydrogenase (2,5-ddol dehydrogenase) P1.orf0509 menElong-chain-fatty-acid--CoA ligase 568030 569610 1 1581 P1.orf0510Acyl-CoA dehydrogenase, middle domain 570905 569721 −3 1185 proteinP1.orf0511 pimeloyl-CoA dehydrogenase 572014 570902 −2 1113 P1.orf0512lcfB AMP-dependent synthetase and ligase 573590 572049 −3 1542P1.orf0513 dctP putative dicarboxylate-binding periplasmic 573957 5750213 1065 protein P1.orf0514 TRAP transporter, DctQ-like membrane 575066575599 2 534 protein P1.orf0515 trap dicarboxylate transporter, dctmsubunit 575642 576946 2 1305 P1.orf0516 Long-chain specific acyl-CoAdehydrogenase 578341 577181 −2 1161 P1.orf0517 acetyl-CoAacetyltransferase 579540 578338 −1 1203 P1.orf0518 conservedhypothetical protein 581018 579570 −3 1449 P1.orf0519 L-carnitinedehydratase/bile acid-inducible 581258 582325 2 1068 protein FP1.orf0520 bcd Acyl-CoA dehydrogenase, C-terminal domain 582357 583523 31167 protein P1.orf0521 Enoyl-CoA hydratase/isomerase 583556 584341 2786 P1.orf0522 alkR transcriptional regulator, AraC family protein585281 584475 −3 807 P1.orf0523 alkyl hydroperoxide reductase/thiolspecific 585348 585887 3 540 antioxidant/Mal allergen P1.orf0524 acsAacetyl-CoA synthetase/AMP-(fatty) acid ligase 587610 585964 −1 1647FadDx P1.orf0525 crt enoyl-CoA hydratase 587849 588775 2 927 P1.orf0526OPR3 NADH: flavin oxidoreductase/NADH oxidase 588859 589962 1 1104P1.orf0527 hypothetical protein 590078 590392 2 315 P1.orf0528 mliCMembrane-bound lysozyme inhibitor of C-type 590488 590883 1 396 lysozymeP1.orf0529 csgA putative short-chain dehydrogenase, 591228 590935 −1 294oxidoreductase P1.orf0530 sdh short-chain dehydrogenase/reductase SDR591650 591237 −3 414 P1.orf0531 LysR family transcriptional regulator591776 592696 2 921 P1.orf0532 hexapaptide repeat-containing transferase592801 593412 1 612 P1.orf0533 xerD possible integrase-like protein594709 593429 −2 1281 P1.orf0534 yxjM putative sensor histidine kinase595024 597258 1 2235 P1.orf0535 devR response regulator 597287 598000 2714 P1.orf0536 conserved hypothetical protein 598984 598019 −2 966P1.orf0537 Putative TRAP transporter large permease 600291 598999 −11293 protein P1.orf0538 putative DctQ (C4-dicarboxylate permease, 600818600288 −3 531 small subunit) P1.orf0539 dctP C4-dicarboxylate-bindingperiplasmic protein 601989 600982 −1 1008 P1.orf0540 crt enoyl-CoAhydratase 602255 603037 2 783 P1.orf0541 tagA ToxR-activated gene Alipoprotein 603169 605823 1 2655 P1.orf0542 yngI AMP-dependentsynthetase and ligase 607530 605857 −1 1674 P1.orf0543 conservedhypothetical protein 608158 607595 −2 564 P1.orf0544 conservedhypothetical protein 608700 608179 −1 522 P1.orf0545 conservedhypothetical protein 609437 608847 −3 591 P1.orf0546 sulfotransferase610594 609725 −2 870 P1.orf0547 Hpr(Ser) kinase/phosphatase 611454610591 −1 864 P1.orf0548 sulfotransferase 611695 612348 1 654 P1.orf0549putative N-acetylglucosaminyltransferase 614343 613219 −1 1125P1.orf0550 pcaK 3-hydroxyphenylpropionic acid 615773 614421 −3 1353P1.orf0551 gentisate 1,2-dioxygenase 616945 615887 −2 1059 P1.orf0552doxA rieske (2Fe—2S) domain protein 617264 616950 −3 315 P1.orf0553aromatic-ring-hydroxylating dioxygenase, beta 617775 617281 −1 495subunit P1.orf0554 bphA aromatic-ring-hydroxylating dioxygenase, 618980617772 −3 1209 alpha subunit P1.orf0555 thcD FAD-dependent pyridinenucleotide-disulphide 619193 620440 2 1248 oxidoreductase P1.orf0556iclR transcriptional regulator 621218 620400 −3 819 P1.orf0557 maiAmaleylacetoacetate isomerase 621321 621962 3 642 P1.orf0558fumarylacetoacetate hydrolase 622007 622696 2 690 P1.orf0559 mauR LysRfamily transcriptional regulator 623860 622940 −2 921 P1.orf0560methylmalonate-semialdehyde dehydrogenase 624014 625513 2 1500P1.orf0561 hypothetical protein 625931 628021 2 2091 P1.orf0562hypothetical protein 628036 628410 1 375 P1.orf0563 peptidase M48,Ste24p 628432 630216 1 1785 P1.orf0564 TRAP transporter solute receptor,TAXI family 630217 631218 1 1002 P1.orf0565 hypothetical protein 631582631779 1 198 P1.orf0566 badR MarR family transcriptional regulator633132 633674 3 543 P1.orf0567 hipO peptidase M20D, amidohydrolase633059 631887 −3 1173 P1.orf0568 conserved hypothetical protein 633807634955 3 1149 P1.orf0569 yogA putative zinc-binding dehydrogenase 635212636231 1 1020 P1.orf0570 lcfB AMP-binding domain protein 636228 638114 31887 P1.orf0571 conserved hypothetical protein 638111 638965 2 855P1.orf0572 TRAP-T family transporter, DctQ (4 TMs) 638962 639525 1 564subunit P1.orf0573 TRAP-T family protein transporter, DctM (12 639522640826 3 1305 TMs) subunit P1.orf0574 phenylacetic aciddegradation-related protein 640842 641276 3 435 P1.orf0575 hypotheticalprotein 641795 641316 −3 480 P1.orf0576 heme receptor 644452 641879 −22574 P1.orf0577 fecR anti-FecI sigma factor, FecR 645589 644660 −2 930P1.orf0578 ECF subfamily RNA polymerase sigma-24 646092 645589 −1 504factor P1.orf0579 hypothetical protein 646794 646489 −1 306 P1.orf0580HPr kinase 647747 646791 −3 957 P1.orf0581 asnH asparagine synthase649648 647747 −2 1902 P1.orf0582 peptidase S45 penicillin amidase 649895652276 2 2382 P1.orf0583 enoyl-CoA hydratase 652483 653304 1 822P1.orf0584 yhgD transcriptional regulator, TetR family 654155 653328 −3828 P1.orf0585 conserved hypothetical protein 654646 654152 −2 495P1.orf0586 hypothetical protein 655893 654871 −1 1023 P1.orf0587 algImembrane bound O-acyl transferase MBOAT 657349 655880 −2 1470 familyprotein P1.orf0588 acpM putative acyl carrier protein 657482 657718 2237 P1.orf0589 conserved hypothetical protein 658665 657730 −1 936P1.orf0590 FkbH like protein 658770 660806 3 2037 P1.orf0591 yafCtranscriptional regulator 661741 660836 −2 906 P1.orf0592 addictionmodule antitoxin 662033 661743 −3 291 P1.orf0593 dinJ addiction moduleantitoxin 662319 662020 −1 300 P1.orf0594 hypothetical protein 662702662454 −3 249 P1.orf0595 conserved hypothetical protein 662739 666431 33693 P1.orf0596 hypothetical protein 666428 667438 2 1011 P1.orf0597conserved hypothetical protein 667452 671111 3 3660 P1.orf0598 HipA-like672445 671177 −2 1269 P1.orf0599 XRE family transcriptional regulator672684 672442 −1 243 P1.orf0600 katE catalase 675002 672882 −3 2121P1.orf0601 yhcV Inosine-5′-monophosphate dehydrogenase 675652 675164 −2489 related protein P1.orf0602 phage SPO1 DNA polymerase-related protein675846 676556 3 711 P1.orf0603 tycC non-ribosomal peptide synthetase676765 682599 1 5835 P1.orf0604 tycC Amino acid adenylation 682572691364 3 8793 P1.orf0605 lgrC Amino acid adenylation 691352 692830 21479 P2.orf0001 fdh alcohol dehydrogenase 1 1170 1 1170 P2.orf0002 Dtail protein D 2205 1204 −1 1002 P2.orf0003 phage Tail Protein X 24322205 −3 228 P2.orf0004 conserved hypothetical protein 3679 2429 −2 1251P2.orf0005 hypothetical protein 5381 3663 −3 1719 P2.orf0006bacteriophage gpE 5834 5529 −3 306 P2.orf0007 FII similar to probablebacteriophage protein 6419 5916 −3 504 P2.orf0008 FI phage tail sheathprotein 7686 6511 −1 1176 P2.orf0009 hypothetical protein 8166 7690 −1477 P2.orf0010 conserved hypothetical protein 8989 8177 −2 813P2.orf0011 conserved hypothetical protein 11125 8993 −2 2133 P2.orf0012gp15 protein 11987 11139 −3 849 P2.orf0013 phage baseplate J-likeprotein 12876 11980 −1 897 P2.orf0014 Baseplate assembly protein W 1325612873 −3 384 P2.orf0015 PAAR 13555 13262 −2 294 P2.orf0016 phagebaseplate assembly protein V 14012 13560 −3 453 P2.orf0017 hypotheticalprotein 14632 14009 −2 624 P2.orf0018 hypothetical protein 15097 14816−2 282 P2.orf0019 amtB ammonium transporter 16723 15371 −2 1353P2.orf0020 tauD Taurine dioxygenase 17879 17037 −3 843 P2.orf0021 classII aldolase/adducin family protein 18717 17953 −1 765 P2.orf0022binding-protein-dependent transport systems 19509 18739 −1 771 innermembrane component P2.orf0023 binding-protein-dependent transportsystems 20273 19491 −3 783 inner membrane component P2.orf0024 ABCtransporter related protein 21163 20270 −2 894 P2.orf0025 NMT1/THI5 likedomain protein 22221 21181 −1 1041 P2.orf0026 metal-dependentphosphohydrolase 22657 23268 1 612 P2.orf0027 lutR transcriptionalregulatory protein 23401 24090 1 690 P2.orf0028 dhkJ Signal transductionhistidine kinase 24694 29427 1 4734 P2.orf0029 rpfG two componentsystem, transcriptional 29424 30527 3 1104 regulatory protein P2.orf0030ydeR major facilitator superfamily protein 31739 30543 −3 1197P2.orf0031 yeaT transcriptional regulator 31835 32716 2 882 P2.orf0032yvdB sulphate transporter 34447 32732 −2 1716 P2.orf0033 XRE familytranscriptional regulator 34788 34528 −1 261 P2.orf0034 conservedhypothetical protein 35012 35473 2 462 P2.orf0035 lmrA transcriptionalregulator, TetR family 35497 36090 1 594 P2.orf0036 ABC transporterrelated protein 36176 38086 2 1911 P2.orf0037 hypothetical protein 3812638839 2 714 P2.orf0038 ABC superfamily ATP binding cassette 40501 38924−2 1578 transporter substrate-binding protein P2.orf0039 poly(asparticacid) hydrolase 41621 40668 −3 954 P2.orf0040 gsiA ABC superfamily ATPbinding cassette 43229 41646 −3 1584 transporter, ABC protein P2.orf0041Dipeptide transport system permease protein 44158 43292 −2 867 dppCP2.orf0042 Putative peptide transport system permease 45096 44155 −1 942protein P2.orf0043 acyl-coenzyme A: 6-aminopenicillanic-acid- 4656445401 −2 1164 acyltransferase P2.orf0044 bphR GntR-familytranscriptional regulator 46705 47880 1 1176 P2.orf0045 hypotheticalprotein 48044 47868 −3 177 P2.orf0046 paiB Protease synthase andsporulation protein PAI 48505 48155 −2 351 P2.orf0047 amidase familyprotein 49974 48520 −1 1455 P2.orf0048 secretion protein HlyD familyprotein 50955 50059 −1 897 P2.orf0049 conserved hypothetical protein51167 50952 −3 216 P2.orf0050 ydhK fusaric acid resistance proteinregion 53257 51164 −2 2094 P2.orf0051 yeaM AraC family transcriptionalregulator 53335 54138 1 804 P2.orf0052 conserved hypothetical protein54665 55303 2 639 P2.orf0053 conserved hypothetical protein 56402 55323−3 1080 P2.orf0054 conserved hypothetical protein 56446 57084 1 639P2.orf0055 conserved hypothetical protein 57088 57966 1 879 P2.orf0056pheC extracellular solute-binding protein 58113 58955 3 843 P2.orf0057glnP polar amino acid ABC transporter, inner 59028 59690 3 663 membranesubunit P2.orf0058 tcyC ABC transporter ATP-binding protein 59687 604722 786 P2.orf0059 Chain A, Crystal Structure Of A Muconate 60482 61603 21122 Cycloisomerase From Azorhizobium Caulinodans P2.orf0060 dadA FADdependent oxidoreductase 61661 62932 2 1272 P2.orf0061 ilvB thiaminepyrophosphate protein central region 62971 64722 1 1752 P2.orf0062 gcvALysR family transcriptional regulator 65673 64735 −1 939 P2.orf0063conserved hypothetical protein 66090 65689 −1 402 P2.orf0064Beta-lactamase 67350 66328 −1 1023 P2.orf0065 Chloramphenicolacetyltransferase 68054 67404 −3 651 P2.orf0066 yoeA MATE efflux familyprotein 69602 68235 −3 1368 P2.orf0067 nccB RND family effluxtransporter MFP subunit 69931 71175 1 1245 P2.orf0068 nolG acriflavinresistance protein 71172 74234 3 3063 P2.orf0069 GCN5-relatedN-acetyltransferase 74551 74243 −2 309 P2.orf0070 yagA ISHne2,transposase 74674 75075 1 402 P2.orf0071 putative periplasmic protein75622 76551 1 930 P2.orf0072 GCN5-related N-acetyltransferase 7851378055 −1 459 P2.orf0073 mannitol transporter 78868 79398 1 531P2.orf0074 DctMS 79403 80728 2 1326 P2.orf0075 dctP putativeextracellular solute-binding protein, 80801 81931 2 1131 family 7P2.orf0076 menE citrate synthase 81954 83537 3 1584 P2.orf0077 enoyl CoAhydratase 83534 84376 2 843 P2.orf0078 marA Multiple antibioticresistance protein marA 84846 84418 −1 429 P2.orf0079 conservedhypothetical protein 85298 84885 −3 414 P2.orf0080 fct TonB-dependentsiderophore receptor 85472 87691 2 2220 P2.orf0081 fes esterase 8768889295 1 1608 P2.orf0082 ytnP metallo-beta-lactamase 90248 89301 −3 948P2.orf0083 nahR Transcriptional regulator, LysR family 90263 91285 21023 P2.orf0084 guaA putative GMP synthase [glutamine- 91310 91993 2 684hydrolyzing] P2.orf0085 conserved hypothetical protein 92830 92006 −2825 P2.orf0086 transcriptional regulator, GntR family 93052 93756 1 705P2.orf0087 dapA Dihydrodipicolinate synthase 93875 94837 2 963P2.orf0088 hypothetical protein 94888 95079 1 192 P2.orf0089 artIextracellular solute-binding protein 95092 95901 1 810 P2.orf0090 yecSABC transporter, membrane spanning protein 95990 96655 2 666 (aminoacid) P2.orf0091 gltK putative amino acid ABC transporter 96667 97320 1654 permease protein P2.orf0092 glnQ putative amino acid ABC transporterATP- 97313 98035 2 723 binding protein P2.orf0093 dadA D-amino-aciddehydrogenase 98035 99276 1 1242 P2.orf0094 proline racemase 99290100291 2 1002 P2.orf0095 Tripartite ATP-independent periplasmic 100453100992 1 540 P2.orf0096 siaT TRAP dicarboxylate transporter, DctMsubunit 100994 102262 2 1269 P2.orf0097 TRAP-type large permeasecomponent 102297 103304 3 1008 P2.orf0098 sugar ABC transporter 105174103435 −1 1740 P2.orf0099 conserved hypothetical protein 105572 105285−3 288 P2.orf0100 yurM binding-protein-dependent transport systems106468 105587 −2 882 inner membrane component P2.orf0101 sugar ABCtransporter 107336 106470 −3 867 P2.orf0102 ABC transporter relatedprotein 108411 107341 −1 1071 P2.orf0103 smoK ABC transporter relatedprotein 109522 108431 −2 1092 P2.orf0104 glpD FAD dependentoxidoreductase 111127 109586 −2 1542 P2.orf0105 conserved hypotheticalprotein 112676 111435 −3 1242 P2.orf0106 hypothetical protein 113264113103 −3 162 P2.orf0107 cydB cytochrome d ubiquinol oxidase, subunit II114258 113248 −1 1011 P2.orf0108 cydA cytochrome bd ubiquinol oxidase,subunit I 115738 114263 −2 1476 P2.orf0109 lcfB AMP-dependent synthetaseand ligase 116321 117895 2 1575 P2.orf0110 putative extracellularsolute-binding protein 117996 119045 3 1050 P2.orf0111 conservedhypothetical protein 119050 119565 1 516 P2.orf0112 siaT TRAP-typeC4-dicarboxylate transport system, 119562 120851 3 1290 large permeasecomponent P2.orf0113 todF alpha/beta hydrolase fold protein 120931121773 1 843 P2.orf0114 conserved hypothetical protein 121793 122113 2321 P2.orf0115 mhpB protocatechuate 4,5-dioxygenase subunit beta 122110122937 1 828 P2.orf0116 bedB CinA3 122951 123274 2 324 P2.orf0117 hcaEAromatic-ring-hydroxylating dioxygenase, 123351 124739 3 1389 alphasubunit-like protein P2.orf0118 3-phenylpropionate dioxygenase subunitbeta 124751 125293 2 543 P2.orf0119 bphB2,3-dihydroxy-2,3-dihydrophenylpropionate 125327 126139 2 813dehydrogenase P2.orf0120 2-nitropropane dioxygenase, NPD 126169 127191 11023 P2.orf0121 todJ 2-oxopent-4-enoate hydratase 127188 127979 3 792P2.orf0122 acetaldehyde dehydrogenase 127985 128923 2 939 P2.orf01234-hydroxy-2-oxovalerate aldolase 128927 129985 2 1059 P2.orf0124 tetRregulatory protein TetR 130566 129976 −1 591 P2.orf0125 thcDferredoxin--NAD+ reductase 130857 132035 3 1179 P2.orf0126 conservedhypothetical protein 132167 132940 2 774 P2.orf0127 ycdT diguanylatecyclase 133040 134215 2 1176 P2.orf0128 yhiN conserved hypotheticalprotein 135416 134226 −3 1191 P2.orf0129 gacS multi-sensor hybridhistidine kinase 135604 139797 1 4194 P2.orf0130 isopenicillin Nsynthase 140642 139803 −3 840 P2.orf0131 hypothetical protein 141315142607 3 1293 P2.orf0132 hypothetical protein 142771 142646 −2 126P2.orf0133 transposase, IS4 143023 142853 −2 171 P2.orf0134 transposase143487 143020 −1 468 P2.orf0135 peptidase M48 Ste24p 145079 144252 −3828 P2.orf0136 rhtB RhtB family transporter 145828 145223 −2 606P2.orf0137 ASN1 putative asparagine synthase (glutamine- 147157 145841−2 1317 hydrolyzing) P2.orf0138 ubiE Generic methyltransferase 148478147132 −3 1347 P2.orf0139 transcriptional regulator, LysR family 149523148594 −1 930 P2.orf0140 ATP-binding protein 150384 149590 −1 795P2.orf0141 dapA Dihydrodipicolinate synthase 151442 150381 −3 1062P2.orf0142 conserved hypothetical protein 152393 151569 −3 825P2.orf0143 conserved hypothetical protein 153423 152380 −1 1044P2.orf0144 putative ABC transporter permease protein 154241 153420 −3822 P2.orf0145 mtrR transcriptional regulatory protein 155062 154388 −2675 P2.orf0146 Pyrimidine precursor biosynthesis enzyme 155238 156239 31002 P2.orf0147 putative transmembrane protein 156889 156278 −2 612P2.orf0148 transcriptional regulator, TetR family 157618 157037 −2 582P2.orf0149 Pirin-related protein 157819 158520 1 702 P2.orf0150dihydroxy-acid dehydratase 158781 160622 3 1842 P2.orf0151NMT1/THI5-like domain-containing protein 160851 161813 3 963 P2.orf0152ABC transporter, membrane spanning protein 161910 162695 3 786P2.orf0153 ABC transporter, nucleotide binding/ATPase 162692 163480 2789 protein P2.orf0154 dinJ addiction module antitoxin 163579 163881 1303 P2.orf0155 addiction module toxin, RelE/StbE family 163878 164165 3288 P2.orf0156 ydaP thiamine pyrophosphate protein domain 165971 164190−3 1782 protein TPP-binding P2.orf0157 putative fatty acid betahydroxylase 166136 167419 2 1284 P2.orf0158 superoxide dismutase protein167454 167984 3 531 P2.orf0159 yidJ arylsulfatase A and related enzyme168829 170619 1 1791 P2.orf0160 pcaC carboxymuconolactone decarboxylase171893 170712 −3 1182 P2.orf0161 transcriptional regulator, LysR family172948 172043 −2 906 P2.orf0162 fdh zinc-containing alcoholdehydrogenase 173105 174121 2 1017 superfamily protein P2.orf0163 fabGshort chain dehydrogenase 174920 174168 −3 753 P2.orf0164 ampR MPR_RHOCARecName: Full = HTH-type 176285 175293 −3 993 transcriptional activatorAmpR emb P2.orf0165 LAC_RHOCA RecName: Full = Beta- 176386 177288 1 903lactamase; AltName: Full = Penicillinase; Flags: Precursor embP2.orf0166 TetR family transcriptional regulator 177876 177292 −1 585P2.orf0167 ykvO 3-oxoacyl-(acyl-carrier-protein) reductase 177978 1787333 756 P2.orf0168 putative transcriptional regulator, AbrB family 179015178761 −3 255 P2.orf0169 DNA-binding protein 179385 179083 −1 303P2.orf0170 conserved hypothetical protein 180464 179562 −3 903P2.orf0171 mngR Mannosyl-D-glycerate transport/metabolism 181469 180681−3 789 system repressor mngR P2.orf0172 cat2 putative 4-hydroxybutyratecoenzyme A 182704 181442 −2 1263 transferase P2.orf0173 MmgE/PrpD familyprotein 182794 184152 1 1359 P2.orf0174 paaK putative aerobicphenylacetate-CoA ligase 184208 185620 2 1413 P2.orf0175 gatAglutamyl-tRNA (Gln) amidotransferase, A 185687 187156 2 1470 subunitP2.orf0176 amiD Putative amidase 188946 187531 −1 1416 P2.orf0177NMT1/THI5 like domain protein 189203 190225 2 1023 P2.orf0178 shortchain dehydrogenase 190353 191204 3 852 P2.orf0179 hisC aspartateaminotransferase 191201 192334 2 1134 P2.orf0180 ytxM abhydrolase 1192352 193233 1 882 P2.orf0181 allC allantoate amidohydrolase 193332194600 3 1269 P2.orf0182 ytbD major facilitator superfamily MFS_1 195827194634 −3 1194 P2.orf0183 hypothetical protein 196724 197392 2 669P2.orf0184 hypothetical protein 197900 197595 −3 306 P2.orf0185 slmATranscriptional regulator 198971 198189 −3 783 P2.orf0186 branched-chainamino acid ABC superfamily 200283 199069 −1 1215 ATP binding cassettetransporter, binding protein P2.orf0187 pyruvate carboxylase 203732200391 −3 3342 P2.orf0188 caiC AMP-dependent synthetase and ligase205403 203736 −3 1668 P2.orf0189 ycdT diguanylate cyclase 205708 2073241 1617 P2.orf0190 2-oxoisovalerate dehydrogenase (alpha 207731 208963 21233 subunit) P2.orf0191 2-oxoisovalerate dehydrogenase (beta 208968209981 3 1014 subunit) P2.orf0192 branched-chain alpha-keto acid 209985211337 3 1353 dehydrogenase subunit E2 P2.orf0193 lpdV dihydrolipoamidedehydrogenase 211342 212739 1 1398 P2.orf0194 iorA isoquinoline1-oxidoreductase subunit alpha 213057 213515 3 459 P2.orf0195 iorBisoquinoline 1-oxidoreductase, beta subunit 213533 215710 2 2178P2.orf0196 yycB QbsM like protein 215917 217068 1 1152 P2.orf0197 nemRTetR family transcriptional regulator 217122 217727 3 606 P2.orf0198rutB putative hydrolase 218483 217788 −3 696 P2.orf0199 ytlAnitrate/sulfonate/taurine/bicarbonate ABC 218671 219723 1 1053superfamily ATP binding cassette transporter, binding protein P2.orf0200ytlC taurine ABC superfamily ATP binding cassette 219761 220600 2 840transporter, ABC protein P2.orf0201 ytlD putative ABC transportproteins, inner 220590 221444 3 855 membrane component P2.orf0202putative membrane protein, DUF81 221536 222270 1 735 P2.orf0203 gloAglyoxalase/bleomycin resistance 222718 222281 −2 438 protein/dioxygenaseP2.orf0204 DoxX family protein 223198 222809 −2 390 P2.orf0205 TetRfamily transcriptional regulator 223870 223322 −2 549 P2.orf0206 alcoholdehydrogenase 223948 224979 1 1032 P2.orf0207 FAD-binding 9siderophore-interacting domain 225757 224999 −2 759 protein P2.orf0208yeaM transcriptional regulatory protein 226721 225882 −3 840 P2.orf0209cyaA ferredoxin 228506 226734 −3 1773 P2.orf0210 nte1 transcriptionalregulator, Crp/Fnr family 228613 229329 1 717 P2.orf0211 patatin 230390229314 −3 1077 P2.orf0212 hypothetical protein 230826 230365 −1 462P2.orf0213 hypothetical protein 231299 230823 −3 477 P2.orf0214 oruRPA2556 232461 231394 −1 1068 P2.orf0215 ycfQ TetR family transcriptionalregulator 233166 232561 −1 606 P2.orf0216 yjgI putative short-chain233301 234071 3 771 dehydrogenase/oxidoreductase P2.orf0217 conservedhypothetical protein 235012 234062 −2 951 P2.orf0218 fhuF ferric ironreductase 235830 235087 −1 744 P2.orf0219 fhuB transporter 237833 235833−3 2001 P2.orf0220 fhuC ABC-type hydroxamate-dependent iron 238609237830 −2 780 transport system, ATPase component P2.orf0221 fhuDperiplasmic component, ABC-type 239544 238606 −1 939hydroxamate-dependent iron transport system P2.orf0222 fctTonB-dependent siderophore receptor 241934 239553 −3 2382 P2.orf0223ompR two component transcriptional regulator, 242808 242068 −1 741winged helix family P2.orf0224 MltA-interacting MipA family protein243592 242822 −2 771 P2.orf0225 envZ two component sensor kinase 243801245078 3 1278 P2.orf0226 putative AraC family transcriptional regulator246018 245083 −1 936 P2.orf0227 NAD-dependent epimerase/dehydratase246162 247058 3 897 P2.orf0228 ydbC putative oxidoreductase 247979247098 −3 882 P2.orf0229 sensor histidine kinase 251372 248049 −3 3324P2.orf0230 response regulator receiver protein 252450 251737 −1 714P2.orf0231 transcriptional regulator/antitoxin, MazE 252681 252908 3 228P2.orf0232 conserved hypothetical protein 254762 252999 −3 1764P2.orf0233 transcriptional regulatory protein 255618 254920 −1 699P2.orf0234 fadK putative acid-CoA ligase 257373 255661 −1 1713P2.orf0235 IivF ABC branched chain amino acid transporter, 258272 257553−3 720 ATPase subunit P2.orf0236 IivG ABC branched chain amino acidtransporter, 259047 258265 −1 783 ATPase subunit P2.orf0237 ABC branchedchain amino acid transporter, 260072 259044 −3 1029 inner membranesubunit P2.orf0238 braD ABC branched chain amino acid transporter,260962 260069 −2 894 inner membrane subunit P2.orf0239 ABC branchedchain amino acid transporter, 262424 261135 −3 1290 periplasmicligandbinding protein P2.orf0240 lcfB AMP-dependent synthetase andligase 264169 262496 −2 1674 P2.orf0241 crt enoyl-CoAhydratase/isomerase 265037 264222 −3 816 P2.orf0242 yngJ acyl-CoAdehydrogenase domain-containing 266230 265109 −2 1122 protein P2.orf0243IVD acyl-CoA dehydrogenase domain-containing 267644 266403 −3 1242protein P2.orf0244 conserved hypothetical protein 268137 267889 −1 249P2.orf0245 old putative ATP-dependent endonuclease of the 268626 2703713 1746 OLD family protein P2.orf0246 conserved hypothetical protein271176 270910 −1 267 P2.orf0247 addiction module antitoxin 270868 270617−2 252 P2.orf0248 hypothetical protein 271312 271533 1 222 P2.orf0249short-chain dehydrogenase/reductase SDR 272518 271616 −2 903 P2.orf0250gylR IclR family transcriptional regulator 273492 272716 −1 777P2.orf0251 TRAP-T family transporter, DctM (12 TMs) 274823 273498 −31326 subunit P2.orf0252 hypothetical protein 275340 274813 −1 528P2.orf0253 conserved hypothetical protein 276464 275328 −3 1137P2.orf0254 conserved hypothetical protein 276581 277462 2 882 P2.orf0255aspartate racemase 277466 278194 2 729 P2.orf0256 OsmC family protein278769 279194 3 426 P2.orf0257 methyl-accepting chemotaxis protein280931 279228 −3 1704 P2.orf0258 conserved hypothetical protein 281540281079 −3 462 P2.orf0259 citE citrate lyase beta chain 282469 281537 −2933 P2.orf0260 pcaR transcriptional regulator 283242 282466 −1 777P2.orf0261 acyltransferase 3 283484 284680 2 1197 P2.orf0262 bamIndoleacetamide hydrolase 286128 284692 −1 1437 P2.orf0263 paiB putativeregulatory protein 286853 286185 −3 669 P2.orf0264 aes putative acetylesterase 287833 286868 −2 966 P2.orf0265 gsiA putative ABC transporterATP-binding protein 289497 287845 −1 1653 P2.orf0266 gsiCbinding-protein-dependent transport systems 291379 290399 −2 981 innermembrane component P2.orf0267 appC binding-protein-dependent transportsystems 290351 289500 −3 852 inner membrane component P2.orf0268 appAtwin-arginine translocation pathway signal 292974 291382 −1 1593P2.orf0269 ghrA putative 2-hydroxyacid dehydrogenase family 294110293178 −3 933 protein. P2.orf0270 yeaM AraC family transcriptionalregulator 294233 295117 2 885 P2.orf0271 putative transcriptionalregulators containing 295330 295127 −2 204 the CopG/Arc/MetJ DNA-bindingdomain P2.orf0272 PRC-barrel domain-containing protein 295879 295508 −2372 P2.orf0273 etrA transcriptional regulator, Crp/Fnr family protein296121 296909 3 789 P2.orf0274 conserved hypothetical protein 297016298263 1 1248 P2.orf0275 conserved hypothetical protein 298260 298787 3528 P2.orf0276 DNA ligase III-like protein 298784 299476 2 693P2.orf0277 HvnC halovibrin 299695 300918 1 1224 P2.orf0278 conservedhypothetical protein 301464 301009 −1 456 P2.orf0279 ybfL transposase,is4 family 301727 302773 2 1047 P2.orf0280 thioesterase-like protein303254 302817 −3 438 P2.orf0281 major facilitator superfamily MFS_1304485 303271 −1 1215 P2.orf0282 conserved hypothetical protein 305396306409 2 1014 P2.orf0283 conserved hypothetical protein 306406 306660 1255 P2.orf0284 serS tRNA synthetase class II (G H P and S) 306703 3077071 1005 P2.orf0285 yrpB 2-nitropropane dioxygenase NPD 307806 308786 3981 P2.orf0286 ASMT O-methyltransferase family 2 308810 309811 2 1002P2.orf0287 aldo/keto reductase family oxidoreductase 310797 309829 −1969 P2.orf0288 short-chain dehydrogenase/reductase SDR 311726 310845 −3882 P2.orf0289 siaT TRAP transporter, DctM subunit 313045 311756 −2 1290P2.orf0290 hypothetical protein 313660 313058 −2 603 P2.orf0291 dctPC4-dicarboxylate-binding periplasmic protein 314806 313769 −2 1038P2.orf0292 conserved hypothetical protein 317076 314899 −1 2178P2.orf0293 acyl-CoA dehydrogenase domain-containing 318311 317280 −31032 protein P2.orf0294 IVD1 acyl-CoA dehydrogenase domain protein319462 318308 −2 1155 P2.orf0295 iclR Transcriptional regulator kdgR319747 320523 1 777 P2.orf0296 paaF putative enoyl-CoA hydratase 320572321369 1 798 P2.orf0297 transcriptional regulator 321550 321849 1 300P2.orf0298 plasmid stabilization system protein 321846 322172 3 327P2.orf0299 lmrB Lincomycin resistance protein 323465 322224 −3 1242P2.orf0300 hypothetical protein 326989 323492 −2 3498 P2.orf0301 alsSacetolactate synthase 327307 328953 1 1647 P2.orf0302 rfbU glycosyltransferase group 1 330096 329038 −1 1059 P2.orf0303 divJ histidinekinase response regulator hybrid 331511 330273 −3 1239 proteinP2.orf0304 hypothetical protein 332022 331756 −1 267 P2.orf0305hypothetical protein 331765 331646 −2 120 P2.orf0306 polC polymeraseepsilon subunit 333137 332193 −3 945 P2.orf0307 conserved hypotheticalprotein 334008 333151 −1 858 P2.orf0308 putative iron reductase 334199334945 2 747 P2.orf0309 RNA polymerase ECF-subfamily sigma-70 334903335475 1 573 factor P2.orf0310 fecR transmembrane sensor 335636 336613 2978 P2.orf0311 acetyl transferase 336764 337423 2 660 P2.orf0312 aatBaspartate aminotransferase 338676 337426 −1 1251 P2.orf0313NAD-dependent aldehyde dehydrogenase 340317 338788 −1 1530 P2.orf0314gcvA transcriptional regulator 341860 342789 1 930 P2.orf0315 conservedhypothetical protein 341730 340687 −1 1044 P2.orf0316 SpoOM familyprotein 343262 343699 2 438 P2.orf0317 pcaI 3-oxoacid CoA-transferase, Asubunit 343828 344538 1 711 P2.orf0318 hypothetical protein 343269343153 −1 117 P2.orf0319 pcaJ 3-oxoadipate CoA-transferase 344535 3452183 684 P2.orf0320 mbtH MbtH-like protein 345567 345812 3 246 P2.orf0321putative SyrP-like regulatory protein 346227 346856 3 630 P2.orf0322putative non-ribosomal peptide synthetase 346919 348349 2 1431P2.orf0323 lgrC amino acid adenylation domain protein 348429 367838 319410 P2.orf0324 lgrC PvdI 367789 369903 1 2115 P2.orf0325 lgrDGRC_BREPA RecName: Full = Linear 369900 381584 3 11685 gramicidinsynthase subunit C; Includes: RecName: Full = ATP-dependent valineadenylase; Short = ValA; AltName: Full = Valine activase; Includes:RecName: Full = ATP- dependent D-valine adenylase; Short = D-ValA;AltName: Full = D-valine P2.orf0326 iucB putative acetylase 381581382612 2 1032 P2.orf0327 pvdA L-ornithine 5-monooxygenase 382609 3839401 1332 P2.orf0328 fhuF ferric iron reductase protein 383944 384714 1 771P2.orf0329 hypothetical protein 384753 385127 3 375 P2.orf0330 pvdQacyl-homoserine lactone acylase 385517 387064 2 1548 P2.orf0331 fctferrichrome-iron transporter 387288 389717 3 2430 P2.orf0332 fhuC ABCtransporter related protein 389797 390591 1 795 P2.orf0333 fhuDperiplasmic binding protein 390613 391512 1 900 P2.orf0334 fhuBiron-hydroxamate transporter permease 391509 393557 3 2049 subunitP2.orf0335 pyoverdine ABC export system, 393612 395282 3 1671permease/ATP-binding protein P2.orf0336 nitrile hydratase-like protein395682 395365 −1 318 P2.orf0337 conserved hypothetical protein 396044395733 −3 312 P2.orf0338 nitrile hydratase 396434 396105 −3 330P2.orf0339 conserved hypothetical protein 396919 396644 −2 276P2.orf0340 glutathione-dependent formaldehyde- 397372 397764 1 393activating GFA P2.orf0341 aceE Pyruvate dehydrogenase(acetyl-transferring) 400219 397808 −2 2412 P2.orf0342 transcriptionalregulator 400360 400839 1 480 P2.orf0343 conserved hypothetical protein402074 401058 −3 1017 P2.orf0344 pstB high-affinity phosphate ABCtransporter ATP- 403461 402658 −1 804 binding protein P2.orf0345 pstAphosphate ABC transporter permease protein 404321 403458 −3 864P2.orf0346 pstC phosphate ABC transporter, inner membrane 405346 404336−2 1011 subunit PstC P2.orf0347 pstS phosphate ABC transporter,periplasmic 406436 405432 −3 1005 phosphate-binding protein P2.orf0348PEPM phosphoenolpyruvate phosphomutase 407744 406830 −3 915 P2.orf0349aspC aminotransferase class I and II 408984 407782 −1 1203 P2.orf0350ABC superfamily ATP binding cassette 410075 409077 −3 999 transporter,binding protein P2.orf0351 fbpB inner membrane component of ABC 411775410132 −2 1644 transporter P2.orf0352 potA ABC transporter relatedprotein 412855 411779 −2 1077 P2.orf0353 trpI transcriptional regulator,LysR family 413003 413887 2 885 P2.orf0354 class II aldolase/adducinfamily protein 414043 414819 1 777 P2.orf0355 yusQ 4-oxalocrotonatetautomerase 415302 414910 −1 393 P2.orf0356 pecT LysR familytranscriptional regulator 415370 416272 2 903 P2.orf0357 fecR putativeFecR 417310 416303 −2 1008 P2.orf0358 fecI RNA polymerase sigma-70family protein 417962 417426 −3 537 P2.orf0359 Sphingosine kinase andenzymes related to 419046 418102 −1 945 eukaryotic diacylglycerol kinaseP2.orf0360 conserved hypothetical protein 421188 419365 −1 1824P2.orf0361 acyl-CoA dehydrogenase domain protein 421331 422548 2 1218P2.orf0362 Putative HPr kinase/phosphorylase 422644 423597 1 954P2.orf0363 siaT TRAP-T family protein transporter, DctM (12 424975423671 −2 1305 TMs) subunit P2.orf0364 conserved hypothetical protein425520 424972 −1 549 P2.orf0365 Bacterial extracellular solute-bindingprotein, 426771 425599 −1 1173 family 7 P2.orf0366 lcfB AMP-dependentsynthetase and ligase 428473 426866 −2 1608 P2.orf0367 paaG enoyl-CoAhydratase/isomerase family protein 429389 428544 −3 846 P2.orf0368acyl-CoA dehydrogenase-like 430563 429403 −1 1161 P2.orf0369 yurK GntRfamily transcriptional regulator 430895 431545 2 651 P2.orf0370 pleCtwo-component hybrid sensor and regulator 431729 433306 2 1578P2.orf0371 degU transcriptional regulator 433932 433294 −1 639P2.orf0372 ugpC lactose transport ATP-binding protein LacK 434169 4353353 1167 P2.orf0373 yurN binding-protein-dependent transport systems435328 436269 1 942 inner membrane component P2.orf0374 yesQbinding-protein-dependent transport systems 436266 437108 3 843 innermembrane component P2.orf0375 sugar binding protein of ABC transporter437160 438434 3 1275 P2.orf0376 icc conserved hypothetical protein438434 439282 2 849 P2.orf0377 conserved hypothetical protein 439858439541 −2 318 P2.orf0378 conserved hypothetical protein 441210 439870 −11341 P2.orf0379 dppD ABC transporter ATP-binding protein 442939 441290−2 1650 P2.orf0380 inner membrane component of binding- 443801 442944 −3858 protein-dependent transport system P2.orf0381 inner membranecomponent of binding- 444744 443806 −1 939 protein-dependent transportsystem P2.orf0382 extracellular solute-binding protein 446347 444758 −21590 P2.orf0383 ybhD HTH-type transcriptional regulator 446504 447463 2960 P2.orf0384 acyI gamma-glutamyltranspeptidase 447601 449319 1 1719P2.orf0385 putative amidotransferase 449371 450792 1 1422 P2.orf0386arylmalonate decarboxylase 451589 450840 −3 750 P2.orf0387 citEHpcH/HpaI aldolase 451750 452613 1 864 P2.orf0388 maoC MaoC-likedehydratase 452668 453186 1 519 P2.orf0389 conserved hypotheticalprotein 453403 453849 1 447 P2.orf0390 hypothetical protein 453982454575 1 594 P2.orf0391 acyl-CoA dehydrogenase domain protein 455977454583 −2 1395 P2.orf0392 yajO aldo/keto reductase 456051 457085 3 1035P2.orf0393 GNAT family acetyltransferase 457600 457082 −2 519 P2.orf0394nitrile hydratase beta-like protein 458132 457743 −3 390 P2.orf0395 nthAalpha subunit of nitrile hydratase 458767 458156 −2 612 P2.orf0396nitrile hydratase beta subunit 459468 458782 −1 687 P2.orf0397 conservedhypothetical protein 460174 459506 −2 669 P2.orf0398 amiC amino acid ABCtransporter 460454 461557 2 1104 P2.orf0399 MarR family transcriptionalregulator 461618 462076 2 459 P2.orf0400 conserved hypothetical protein462391 462104 −2 288 P2.orf0401 acrR TetR family transcriptionalregulator 463014 462388 −1 627 P2.orf0402 Phytoene dehydrogenase andrelated protein 464630 463011 −3 1620 P2.orf0403 aldehyde dehydrogenase464849 466288 2 1440 P2.orf0404 Amidase 467917 466403 −2 1515 P2.orf0405IivF ABC superfamily ATP binding cassette 468812 468099 −3 714transporter, ABC protein P2.orf0406 braF urea or short-chain amide ABCtransporter 469557 468814 −1 744 P2.orf0407 urea or short-chain amideABC transporter 470626 469559 −2 1068 P2.orf0408 IivH urea orshort-chain amide ABC transporter 471496 470630 −2 867 P2.orf0409 amiCputative Leu/Ile/Val/Thr/Ala-binding protein 472766 471513 −3 1254P2.orf0410 amiC ABC transporter substrate-binding protein 474013 472976−2 1038 P2.orf0411 yhbI transcriptional regulatory protein 474233 4746522 420 P2.orf0412 gamma-aminobutyraldehyde dehydrogenase 476115 474658 −11458 P2.orf0413 yybE putative LysR-family regulatory protein 477038476112 −3 927 P2.orf0414 puuC aldehyde dehydrogenase protein 478605477100 −1 1506 P2.orf0415 conserved hypothetical protein 478912 478616−2 297 P2.orf0416 adh alcohol dehydrogenase protein 479205 480242 3 1038P2.orf0417 sdpR ArsR family transcriptional regulator 480297 480611 3315 P2.orf0418 conserved hypothetical protein 480608 480940 2 333P2.orf0419 ATP-dependent Clp protease proteolytic 480987 481583 3 597subunit P2.orf0420 yddV diguanylate cyclase 481691 483121 2 1431P2.orf0421 gabD Aldehyde dehydrogenase (NAD(+)) 483197 484633 2 1437P2.orf0422 bphR transcriptional regulator protein 485453 484707 −3 747P2.orf0423 siaT TRAP dicarboxylate transporter, DctQ subunit 485577486194 3 618 P2.orf0424 yiaN TRAP transporter, DctM subunit subfamily486191 487462 2 1272 P2.orf0425 putative hydroxlacyl-CoA dehydrogenase487462 488415 1 954 P2.orf0426 trap dicarboxylate transporter- dctpsubunit 488412 488996 3 585 P2.orf0427 trap dicarboxylate transporter-dctp subunit 489025 489384 1 360 P2.orf0428 catA oxidoreductase protein489463 490338 1 876 P2.orf0429 bphC glyoxalase/bleomycin resistance490335 491192 3 858 protein/dioxygenase P2.orf0430 decarboxylase protein491197 491901 1 705 P2.orf0431 dmpC 2-hydroxymuconic semialdehyde 491907493361 3 1455 dehydrogenase P2.orf0432 hpcG 2-oxo-hepta-3-ene-1,7-dioicacid hydratase 493381 494163 1 783 P2.orf0433 hpcH HpcH/HpaI aldolase494173 494940 1 768 P2.orf0434 enoyl-CoA hydratase/isomerase familyprotein 495939 496742 3 804 P2.orf0435 phenylacetic acid degradationoperon negative 495806 495021 −3 786 regulatory protein P2.orf0436 menEferuloyl-CoA synthetase 496838 498403 2 1566 P2.orf0437 bphR GntR familytranscriptional regulator 499406 498528 −3 879 P2.orf0438 gentisate1,2-dioxygenase 499618 500733 1 1116 P2.orf04395-carboxymethyl-2-hydroxymuconate Delta- 500863 501768 1 906 isomeraseP2.orf0440 extracellular ligand-binding receptor 501956 503143 2 1188P2.orf0441 braD ABC superfamily ATP binding cassette 503251 504168 1 918transporter, membrane protein P2.orf0442 IivM ABC superfamily ATPbinding cassette 504180 505202 3 1023 transporter, permease proteinP2.orf0443 braF ABC transporter related protein 505195 505962 1 768P2.orf0444 IivF branched-chain amino acid ABC superfamily 505962 5066573 696 ATP binding cassette transporter, ABC protein P2.orf0445 NIT4nitrilase 506670 507617 3 948 P2.orf0446 conserved hypothetical protein507630 508274 3 645 P2.orf0447 putative esterase/lipase 508271 509167 2897 P2.orf0448 hypothetical protein 509275 509907 1 633 P2.orf0449transcriptional activator FtrB 510630 509926 −1 705 P2.orf0450 narKnitrite transporter 510877 513570 1 2694 P2.orf0451 narG nitratereductase, alpha subunit 513598 517338 1 3741 P2.orf0452 narY nitratereductase, beta subunit 517335 518873 3 1539 P2.orf0453 narJ nitratereductase molybdenum cofactor 518878 519603 1 726 assembly chaperoneP2.orf0454 narI respiratory nitrate reductase, gamma subunit 519603520349 3 747 P2.orf0455 nifM PpiC-type peptidyl-prolyl cis-transisomerase 520362 521252 3 891 P2.orf0456 Hemerythrin HHE cation bindingdomain 521300 521854 2 555 subfamily P2.orf0457 moeA molybdopterinbiosynthesis protein 521851 523119 1 1269 P2.orf0458 molybdenum ABCtransporter ATP-binding 523116 523604 3 489 protein P2.orf0459 moaAmolybdenum cofactor biosynthesis protein A 523641 524687 3 1047P2.orf0460 mog molybdopterin binding domain protein 524716 525222 1 507P2.orf0461 NnrS family protein 525243 526451 3 1209 P2.orf0462 Cellulosesynthesis regulatory protein 526976 526461 −3 516 P2.orf0463 diguanylatecyclase 527952 526945 −1 1008 P2.orf0464 aapP General L-amino acidtransport ATP-binding 528809 528063 −3 747 protein P2.orf0465 yhdY aminoacid ABC transporter permease 531132 528817 −1 2316 P2.orf0466 aapJgeneral L-amino acid transport system 532175 531132 −3 1044substrate-binding protein P2.orf0467 dapA dihydrodipicolinate synthase533301 532393 −1 909 P2.orf0468 yibF glutathione S-transferase 534025533423 −2 603 P2.orf0469 dadA D-amino-acid dehydrogenase 535329 534022−1 1308 P2.orf0470 ordL Glycine/D-amino acid oxidase 536660 535332 −31329 P2.orf0471 nocR transcriptional regulator, LysR family 537755536838 −3 918 P2.orf0472 amiC urea ABC transporter, urea binding protein537992 539263 2 1272 P2.orf0473 braD urea ABC transporter, permeaseprotein UrtB 539469 540398 3 930 P2.orf0474 urea ABC transporter,permease protein UrtC 540403 541587 1 1185 P2.orf0475 braF urea ABCtransporter, ATP-binding protein 541602 542384 3 783 UrtD P2.orf0476IivF putative ABC transporter ATP-binding 542458 543147 1 690 componentP2.orf0477 fmdA formamidase 543426 544661 3 1236 P2.orf0478 fmdB FmdBfamily regulatory protein 544730 545059 2 330 P2.orf0479 conservedhypothetical protein 545144 545584 2 441 P2.orf0480 conservedhypothetical protein 545695 546876 1 1182 P2.orf0481 conservedhypothetical protein 546881 548323 2 1443 P2.orf0482 conservedhypothetical protein 548442 549665 3 1224 P2.orf0483 ABC transporterrelated protein 549662 551452 2 1791 P2.orf0484 transcriptionalregulator, MarR family 551531 552130 2 600 P2.orf0485 dipZthiol-disulfide isomerase and thioredoxins 552180 552671 3 492P2.orf0486 suhR Patatin 554481 552685 −1 1797 P2.orf0487 gcvAtranscriptional regulator 555545 554538 −3 1008 P2.orf0488glyoxalase/bleomycin resistance 555678 556124 3 447 protein/dioxygenasesuperfamily protein P2.orf0489 cya calcium binding hemolysin protein556302 558818 3 2517 P2.orf0490 yodM phosphoesterase PA-phosphataserelated 559494 558808 −1 687 protein P2.orf0491 dppCbinding-protein-dependent transport systems 560476 559592 −2 885 innermembrane component P2.orf0492 putative oligopeptide ABC transporter561429 560476 −1 954 (permease) P2.orf0493 gsiB extracellularsolute-binding protein family 5 563119 561512 −2 1608 P2.orf0494 ykfDoligopeptide/dipeptide ABC transporter, ATP- 564228 563212 −1 1017binding protein-like P2.orf0495 oligopeptide/dipeptide ABC transporter,ATP- 565220 564225 −3 996 binding protein-like P2.orf0496 caiCAMP-dependent synthetase and ligase 566971 565217 −2 1755 P2.orf0497conserved hypothetical protein 567938 567177 −3 762 P2.orf0498 B3/4domain protein 568082 568798 2 717 P2.orf0499 hypothetical protein569114 569269 2 156 P2.orf0500 conserved hypothetical protein 569625569290 −1 336 P2.orf0501 hypothetical protein 569983 569669 −2 315P2.orf0502 phytanoyl-CoA dioxygenase (PhyH) family 570940 570065 −2 876protein P2.orf0503 oruR AraC family transcriptional regulator 571040572080 2 1041 P2.orf0504 glpR glycerol-3-phosphate regulon repressor572197 572976 1 780 P2.orf0505 mhpD fumarylacetoacetate (FAA) hydrolase573801 572995 −1 807 P2.orf0506 opdE major facilitator transporter575026 573917 −2 1110 P2.orf0507 major facilitator superfamily MFS_1575433 575083 −1 351 P2.orf0508 regulatory protein 575710 576603 1 894P2.orf0509 transcriptional regulator 577116 576613 −1 504 P2.orf0510short chain dehydrogenase/reductase family 577227 577991 3 765oxidoreductase P2.orf0511 2-hydroxychromene-2-carboxylate isomerase578006 578614 2 609 P2.orf0512 conserved hypothetical protein 578810578622 −3 189 P2.orf0513 AraC family transcriptional regulator 579873578893 −1 981 P2.orf0514 conserved hypothetical protein 580238 579909 −3330 P2.orf0515 dke1 acetylacetone-cleaving enzyme 580708 580235 −2 474P2.orf0516 Glo1 Lactoylglutathione lyase 580954 581478 1 525 P2.orf0517GntR family transcriptional regulator 581586 582263 3 678 P2.orf0518coaA pantothenate kinase 582287 583222 2 936 P2.orf0519 alkJ GMC familyoxidoreductase 583334 584968 2 1635 P2.orf0520 LysR familytranscriptional regulator 585483 584983 −1 501 P2.orf0521 yhaJ LysRfamily transcriptional regulator 585890 585480 −3 411 P2.orf0522 TrpRbinding protein WrbA 585995 586621 2 627 P2.orf0523 TPRdomain-containing protein 588934 587846 −2 1089 P2.orf0524 caiCAMP-dependent synthetase and ligase 590679 589033 −1 1647 P2.orf0525putative monooxygenase 592736 590775 −3 1962 P2.orf0526 Beta-lactamase593031 594248 3 1218 P2.orf0527 TRAP-T family transporter, DctP(periplasmic 594350 595498 2 1149 binding) subunit P2.orf0528 TRAP-Tfamily transporter, DctQ (4 TMs) 595572 596105 3 534 subunit P2.orf0529siaT TRAP-T family transporter, DctM (12 TMs) 596102 597409 2 1308subunit P2.orf0530 regulatory protein, TetR 597461 598171 2 711P2.orf0531 putative transcriptional regulators 598216 598461 1 246P2.orf0532 ybiO mscS family protein ybiO 600759 598477 −1 2283P2.orf0533 Beta-lactamase 600931 602205 1 1275 P2.orf0534 putativePeroxiredoxin 602334 602972 3 639 P2.orf0535 conserved hypotheticalprotein 603028 603453 1 426 P2.orf0536 conserved hypothetical protein603785 603438 −3 348 P2.orf0537 rob putative transcriptional regulatorprotein, AraC 604467 605375 3 909 family P2.orf0538 yebQ majorfacilitator superfamily MFS_1 606706 605393 −2 1314 P2.orf0539 glxA AraCfamily transcriptional regulator 608808 607756 −1 1053 P2.orf0540 soxBsarcosine oxidase beta subunit family protein 608908 610161 1 1254P2.orf0541 soxD sarcosine oxidase delta subunit family protein 610172610471 2 300 P2.orf0542 soxA sarcosine oxidase alpha subunit familyprotein 610468 613470 1 3003 P2.orf0543 soxG sarcosine oxidase, gammasubunit 613489 614040 1 552 P2.orf0544 hypothetical protein 614270614079 −3 192 P2.orf0545 conserved hypothetical protein 614962 614510 −2453 P2.orf0546 leuD 3-isopropylmalate dehydratase, small subunit 615722615069 −3 654 P2.orf0547 leuC adenosylcobinamide 617121 615727 −1 1395kinase/adenosylcobinamide-phosphate guanylyltransferase P2.orf0548L-carnitine dehydratase/bile acid-inducible 618356 617118 −3 1239protein F P2.orf0549 GCDH glutaryl-CoA dehydrogenase 619608 618421 −11188 P2.orf0550 isochorismatase hydrolase 620279 619656 −3 624P2.orf0551 hyuA hydantoin utilization protein A 622363 620231 −2 2133P2.orf0552 5-oxoprolinase (ATP-hydrolyzing) 624071 622392 −3 1680P2.orf0553 yvoA GntR family transcriptional regulator 625002 624157 −1846 P2.orf0554 Conserved hypothetical protein 626913 625399 −1 1515P2.orf0555 conserved hypothetical protein 628844 627255 −3 1590P2.orf0556 glyoxalase/bleomycin resistance 629129 629605 2 477protein/dioxygenase P2.orf0557 sdpR ArsR family transcriptionalregulator 629602 629943 1 342 P2.orf0558 activator of HSP90 ATPase 1family protein 629940 630437 3 498 P2.orf0559 uhpA LuxR familytranscriptional regulator 630987 630421 −1 567 P2.orf0560 acetyl-CoAacetyltransferase 631151 632326 2 1176 P2.orf0561 menE putativelong-chain-fatty-acid CoA ligase 632336 633874 2 1539 P2.orf0562 hutCtranscriptional regulator, GntR family 634557 633829 −1 729 P2.orf0563conserved hypothetical protein 634942 634649 −2 294 P2.orf0564 cda1polysaccharide deacetylase 635146 636012 1 867 P2.orf0565 TRAPtransporter, transmembrane protein 636092 636685 2 594 P2.orf0566Putative TRAP transporter large permease 636682 637965 1 1284 proteinP2.orf0567 yiaO putative exported protein (TRAP-type transport 638073639077 3 1005 system, periplasmic component) P2.orf0568 IRG1 MmgE/PrpD639094 640503 1 1410 P2.orf0569 mmgC acyl-CoA dehydrogenase 640689641858 3 1170 P2.orf0570 L-carnitine dehydratase/bile acid-inducible641855 643117 2 1263 protein F P2.orf0571 yfdE L-carnitinedehydratase/bile acid-inducible 643436 644209 2 774 protein F P2.orf0572pilJ putative methyl accepting chemotaxis protein 644419 646494 1 2076P2.orf0573 conserved hypothetical protein 647362 646472 −2 891P2.orf0574 peptide deformylase 647567 648151 2 585 P2.orf0575 shortchain dehydrogenase 649016 648183 −3 834 P2.orf0576 ycaN LysR familytranscriptional regulator 649114 650016 1 903 P2.orf0577 ycfQ TetRfamily transcriptional regulator 650709 650032 −1 678 P2.orf0578 yjgIshort-chain dehydrogenase/reductase SDR 650818 651558 1 741 P2.orf0579hypothetical protein 652126 652260 1 135 P2.orf0580 putativeKetopantoate reductase PanE/ApbA 652662 652390 −1 273 P2.orf0581 leuA2-isopropylmalate synthase 654186 652687 −1 1500 P2.orf0582 hypotheticalprotein 654661 654545 −2 117 P2.orf0583 conserved hypothetical protein655211 654687 −3 525 P2.orf0584 pleC Sensor protein 655466 656701 2 1236P2.orf0585 conserved hypothetical protein 657778 656801 −2 978P2.orf0586 sporulation initiation inhibitor protein Soj 658949 659989 21041 P2.orf0587 chromosome partitioning protein ParB 659989 660891 1 903P2.orf0588 GCN5-related N-acetyltransferase 661556 661014 −3 543P2.orf0589 padR transcriptional regulator 662176 662706 1 531 P2.orf0590conserved hypothetical protein 663239 662793 −3 447 P2.orf0591 fadHNADH: flavin oxidoreductase/NADH oxidase 665393 663282 −3 2112P2.orf0592 triacylglycerol lipase superfamily protein 666816 665485 −11332 P2.orf0593 glxA AraC family transcription regulator 667905 666955−1 951 P2.orf0594 conserved hypothetical protein 668034 668333 3 300P2.orf0595 conserved hypothetical protein 668330 668737 2 408 P2.orf0596yfiR regulatory protein TetR 669425 668724 −3 702 P2.orf0597 putativeacyltransferase 669523 670626 1 1104 P2.orf0598 putative outer membraneautotransporter 672954 670636 −1 2319 barrel P2.orf0599 hypotheticalprotein 687767 686256 −3 1512 P2.orf0600 Invasion protein B homolog688434 687865 −1 570 P2.orf0601 universal stress protein UspA 689603688716 −3 888 P2.orf0602 conserved hypothetical protein 689981 689637 −3345 P3.orf0001 istA Integrase, catalytic region 1 1509 1 1509 P3.orf0002istB IstB domain protein ATP-binding protein 1496 2287 2 792 P3.orf0003CzcC family heavy metal RND efflux outer 3597 2500 −1 1098 membraneprotein P3.orf0004 transposase, IS4 family protein 4330 4806 1 477P3.orf0005 alsR regulatory protein BphR 7279 6389 −2 891 P3.orf0006 copKCopper resistance protein K 7696 7511 −2 186 P3.orf0007 copD putativecopper resistance D transmembrane 8850 7924 −1 927 protein P3.orf0008copC copper resistance protein CopC 9241 8855 −2 387 P3.orf0009 silPheavy metal translocating P-type ATPase 11634 9382 −1 2253 P3.orf0010conserved hypothetical protein 12523 12227 −2 297 P3.orf0011 copB copperresistance protein B 13520 12750 −3 771 P3.orf0012 pcoA CopA familycopper resistance protein 15354 13756 −1 1599 P3.orf0013 cusR twocomponent response regulator 15899 16603 2 705 P3.orf0014 cusS sensorhistidine kinase 16857 18011 3 1155 P3.orf0015 ArsR familytranscriptional regulator 18941 19288 2 348 P3.orf0016 yqcKlactoylglutathione lyase 19302 19772 3 471 P3.orf0017 arsC arsenatereductase 19785 20282 3 498 P3.orf0018 putative sodium bile acidsymporter family 20293 21378 1 1086 protein P3.orf0019 arsC arsenatereductase 21393 21815 3 423 P3.orf0020 ywzG transcriptional regulator,PadR family 21914 22216 2 303 P3.orf0021 chromate transporter 2221323442 1 1230 P3.orf0022 yhbS acetyltransferase, gnat family 23448 239783 531 P3.orf0023 yubM ParB domain protein nuclease 25667 24186 −3 1482P3.orf0024 bepE transporter, HAE1 family 26377 25976 −2 402 P3.orf0025bepF multidrug efflux RND transporter, membrane 27438 26470 −1 969fusion protein MexE P3.orf0026 relaxase 29882 27960 −3 1923 P3.orf0027conserved hypothetical protein 30123 30749 3 627 P3.orf0028 RES domainsuperfamily 30762 31394 3 633 P3.orf0029 conserved hypothetical protein31479 31844 3 366 P3.orf0030 conserved hypothetical protein 33376 31856−2 1521 P3.orf0031 conserved hypothetical protein 33749 33390 −3 360P3.orf0032 conserved hypothetical protein 35140 33746 −2 1395 P3.orf0033conserved hypothetical protein 36100 35150 −2 951 P3.orf0034 conservedhypothetical protein 36489 36097 −1 393 P3.orf0035 DNA repair proteinRadC 37202 36708 −3 495 P3.orf0036 dsba oxidoreductase 37806 37378 −1429 P3.orf0037 conserved hypothetical protein 41061 38170 −1 2892P3.orf0038 conserved hypothetical protein 41510 41061 −3 450 P3.orf0039conserved hypothetical protein 42909 41491 −1 1419 P3.orf0040 putativesecreted protein 43810 42899 −2 912 P3.orf0041 putative secreted protein44499 43807 −1 693 P3.orf0042 conserved hypothetical protein 44894 44496−3 399 P3.orf0043 conserved hypothetical protein 45265 44906 −2 360P3.orf0044 conserved hypothetical protein 45515 45282 −3 234 P3.orf0045plasmid conserved hypothetical protein, 45895 45512 −2 384 RAQPRD familyP3.orf0046 excisionase/Xis, DNA-binding 46099 46569 1 471 P3.orf0047conserved hypothetical protein 46638 47141 3 504 P3.orf0048 AAA ATPase,central region 47159 48073 2 915 P3.orf0049 conserved hypotheticalprotein 48537 49037 3 501 P3.orf0050 conserved hypothetical protein49037 49939 2 903 P3.orf0051 F440523_63 hypothetical protein 50110 507031 594 P3.orf0052 conserved hypothetical protein 50770 53337 1 2568P3.orf0053 conserved hypothetical protein 54127 53378 −2 750 P3.orf0054conserved hypothetical protein 56190 54124 −1 2067 P3.orf0055 conservedhypothetical protein 56638 56321 −2 318 P3.orf0056 lytictransglycosylase, catalytic 57435 56866 −1 570 P3.orf0057 conservedhypothetical protein 58175 57438 −3 738 P3.orf0058 conservedhypothetical protein 58832 58188 −3 645 P3.orf0059 conservedhypothetical protein 59428 58829 −2 600 P3.orf0060 JBP2 helicase-likeprotein 61846 59567 −2 2280 P3.orf0061 conserved hypothetical protein62288 61983 −3 306 P3.orf0062 conserved hypothetical protein 62698 62378−2 321 P3.orf0063 conserved hypothetical protein 63858 62749 −1 1110P3.orf0064 conserved hypothetical protein 64570 63923 −2 648 P3.orf0065conserved hypothetical protein 64907 64647 −3 261 P3.orf0066 conservedhypothetical protein 65331 64924 −1 408 P3.orf0067 conservedhypothetical protein 66560 65871 −3 690 P3.orf0068 yubP conservedhypothetical protein 67482 66655 −1 828 P3.orf0069 conservedhypothetical protein 68533 67628 −2 906 P3.orf0070 conservedhypothetical protein 69128 68844 −3 285 P3.orf0071 conservedhypothetical protein 69696 69436 −1 261 P3.orf0072 tnpA Transposase Tn370407 69766 −1 642 P3.orf0073 merA mercuric reductase 72470 70782 −31689 P3.orf0074 merP mercuric transport protein periplasmic protein72786 72481 −1 306 P3.orf0075 merT putative mercuric transport protein73149 72799 −1 351 P3.orf0076 merR MerR family transcriptional regulator73221 73628 3 408 P3.orf0077 conserved hypothetical protein 74714 73890−3 825 P3.orf0078 conserved hypothetical protein 75281 75003 −3 279P3.orf0079 conserved hypothetical protein 76115 75378 −3 738 P3.orf0080conserved hypothetical protein 76721 76329 −3 393 P3.orf0081 conservedhypothetical protein 77461 77294 −2 168 P3.orf0082 lspA signal peptidaseII 78322 77942 −2 381 P3.orf0083 cadA heavy metal translocating P-typeATPase 81247 78446 −2 2802 P3.orf0084 zntR MerR family transcriptionalregulator 81450 81848 3 399 P3.orf0085 sterol desaturase-like protein82512 82303 −1 210 P3.orf0086 cation efflux protein 82924 83559 1 636P3.orf0087 topB DNA topoisomerase III 86313 84283 −1 2031 P3.orf0088 ssbsingle-stranded DNA-binding protein 87037 86597 −2 441 P3.orf0089conserved hypothetical protein 87638 87111 −3 528 P3.orf0090 conservedhypothetical protein 88426 87635 −2 792 P3.orf0091 conservedhypothetical protein 89902 88856 −2 1047 P3.orf0092 conservedhypothetical protein 90658 90098 −2 561 P3.orf0093 conservedhypothetical protein 92352 90673 −1 1680 P3.orf0094 conservedhypothetical protein 92614 92345 −2 270 P3.orf0095 cobyrinic acida,c-diamide synthase 93473 92598 −3 876 P3.orf0096 phage-related protein93728 93516 −3 213 P3.orf0097 conserved hypothetical protein 94593 93847−1 747 P3.orf0098 conserved hypothetical protein 95428 95544 1 117P3.orf0099 hypothetical protein 95955 96197 3 243 P3.orf0100 iapAlkaline phosphatase isozyme conversion 98224 96218 −2 2007 proteinP3.orf0101 Pyridoxamine 5′-phosphate oxidase-like, FMN- 99403 98414 −2990 binding P3.orf0102 trpB tryptophan synthase subunit beta 100406100089 −3 318 P3.orf0103 trpB tryptophan synthase, beta subunit 101314100406 −2 909 P3.orf0104 conserved hypothetical protein 102640 101558 −21083 P3.orf0105 conserved hypothetical protein 103201 102659 −2 543P3.orf0106 rpoD RNA polymerase sigma factor 105716 103623 −3 2094P3.orf0107 dnaG DNA primase 107778 105883 −1 1896 P3.orf0108 GatB/Yqeydomain superfamily 108256 107789 −2 468 P3.orf0109 carAcarbamoyl-phosphate synthase small chain 108790 109962 1 1173 P3.orf0110carB Carbamoylphosphate synthase large subunit 109955 113200 2 3246P3.orf0111 greA transcription elongation factor GreA 113454 113912 3 459P3.orf0112 conserved hypothetical protein 115711 113996 −2 1716P3.orf0113 abrB membrane protein AbrB duplication 116368 115796 −2 573P3.orf0114 lrp transcriptional regulator 117507 117001 −1 507 P3.orf0115nucleoside-diphosphate-sugar epimerase 117815 118876 2 1062 P3.orf0116trxB thioredoxin reductase (NADPH) 118974 119945 3 972 P3.orf0117 yafCredox-sensitive transcriptional activator 120106 120996 1 891 P3.orf0118diguanylate cyclase 121882 121061 −2 822 P3.orf0119 conservedhypothetical protein 122308 121898 −2 411 P3.orf0120 conservedhypothetical protein 122847 122305 −1 543 P3.orf0121 Serine phosphataseRsbU, regulator of sigma 124565 122895 −3 1671 subunit P3.orf0122 aminoacid ABC transporter periplasmic 125412 124600 −1 813 protein P3.orf0123yedY Sulfoxide reductase catalytic subunit yedY 127668 126661 −1 1008P3.orf0124 aatA aspartate aminotransferase 129076 127874 −2 1203P3.orf0125 uvrB excinuclease ABC subunit B 129371 131581 2 2211P3.orf0126 mdtA RND family efflux transporter MFP subunit 131809 1329121 1104 P3.orf0127 AcrB/AcrD/AcrF family protein 132944 136144 2 3201P3.orf0128 fabG 3-oxoacyl-[acyl-carrier-protein] reductase 136284 1370273 744 P3.orf0129 folB dihydroneopterin aldolase family protein 137128137535 1 408 P3.orf0130 uvrC Nuclease subunit of the excinucleasecomplex 137920 140361 1 2442 P3.orf0131 pgsAPhosphatidylglycerophosphate synthase 140437 140988 1 552 P3.orf0132moaE molybdenum cofactor biosynthesis protein E 141003 141761 3 759P3.orf0133 Calcium-binding EF-hand protein 141891 142391 3 501P3.orf0134 sigW RNA polymerase sigma factor 142388 142996 2 609P3.orf0135 conserved hypothetical protein 142996 143559 1 564 P3.orf0136conserved hypothetical protein 143556 143957 3 402 P3.orf0137 azoB NmrAfamily protein 144927 144049 −1 879 P3.orf0138 yybR transcriptionalregulator 145036 145386 1 351 P3.orf0139 ilvE branched-chain amino acidaminotransferase 146392 145484 −2 909 P3.orf0140 petP transcriptionalregulator, MarR family 146478 146960 3 483 P3.orf0141 petR Responseregulator receiver: Transcriptional 147017 147763 2 747 regulatoryprotein, C-terminal P3.orf0142 envZ Signal transduction histidine kinase147784 149145 1 1362 P3.orf0143 hypothetical protein 149529 149149 −1381 P3.orf0144 yhcK diguanylate cyclase 150423 149653 −1 771 P3.orf0145csaA chaperonin csaA 150861 150505 −1 357 P3.orf0146 magnetic particlemembrane specific GTPase 151425 151000 −1 426 P16 P3.orf0147 conservedhypothetical protein 152319 151645 −1 675 P3.orf0148 rpoH RNA polymerasefactor sigma-32 152518 153435 1 918 P3.orf0149 yuxG oxidoreductase yuxG155553 153502 −1 2052 P3.orf0150 mtnA Initiation factor 2B related156703 155603 −2 1101 P3.orf0151 mtnP Purine phosphorylase, family 2157654 156734 −2 921 P3.orf0152 Cytochrome c1, heme protein precursor158759 157995 −3 765 P3.orf0153 petB Cytochrome b/b6-like protein 159988158759 −2 1230 P3.orf0154 petA ubiquinol-cytochrome c reductase,iron-sulfur 160561 160016 −2 546 subunit P3.orf0155 yibK putativeaminotransferase/methyltransferase 161030 161545 2 516 P3.orf0156 hemFcoproporphyrinogen III oxidase 161542 162483 1 942 P3.orf0157hypothetical protein 162813 162550 −1 264 P3.orf0158 major facilitatortransporter 163774 162830 −2 945 P3.orf0159 hypothetical protein 163909164841 1 933 P3.orf0160 polynucleotide adenylyltransferase region 166208164859 −3 1350 P3.orf0161 hypothetical protein 166528 166259 −2 270P3.orf0162 nudL NUDIX hydrolase 167248 166538 −2 711 P3.orf0163conserved hypothetical protein 167916 167245 −1 672 P3.orf0164 nudFMutT/nudix family protein 168572 167877 −3 696 P3.orf0165 aidB putativeacyl-CoA dehydrogenase 170347 168665 −2 1683 P3.orf0166 Transcriptionalregulator 171179 170394 −3 786 P3.orf0167 hypothetical protein 171425171742 2 318 P3.orf0168 conserved hypothetical protein 171819 172907 31089 P3.orf0169 YcaO-like family 173005 174360 1 1356 P3.orf0170Putative nitroreductase 174395 175204 2 810 P3.orf0171 hypotheticalprotein 175281 177491 3 2211 P3.orf0172 conserved hypothetical protein177491 177958 2 468 P3.orf0173 ywmD von Willebrand factor type A 179423178047 −3 1377 P3.orf0174 transcriptional regulator, LuxR 181216 179585−2 1632 family/hydrolase, alpha/beta fold family P3.orf0175 yeeAPutative DNA methyltransferase yeeA 183092 185971 2 2880 P3.orf0176 LuxRfamily transcriptional regulator 187094 186264 −3 831 P3.orf0177SpoVT/AbrB domain-containing protein 187255 187404 1 150 P3.orf0178 ispE4-diphosphocytidyl-2C-methyl-D-erythritol 188600 187665 −3 936 kinaseP3.orf0179 TPR repeat-containing protein 190357 188618 −2 1740P3.orf0180 conserved hypothetical protein 190896 191087 3 192 P3.orf0181hypothetical protein 193001 191250 −3 1752 P3.orf0182 hypotheticalprotein 193258 193461 1 204 P3.orf0183 peptidase M24 195602 193539 −32064 P3.orf0184 prmA ribosomal protein L11 methylase 196575 195634 −1942 P3.orf0185 uvrD UvrD/REP helicase domain protein 198952 196559 −22394 P3.orf0186 conserved hypothetical protein 200143 199112 −2 1032P3.orf0187 asnO Asparagine synthase (glutamine-hydrolyzing) 202308200392 −1 1917 P3.orf0188 yeaM pyridoxamine 5′-phosphate oxidase-related203165 202365 −3 801 protein P3.orf0189 pyridoxamine 5′-phosphateoxidase-related 203259 203870 3 612 FMN-binding P3.orf0190 conservedhypothetical protein 203949 204098 3 150 P3.orf0191 yfkN 5′-nucleotidase205696 204164 −2 1533 P3.orf0192 pentapeptide repeat-containing protein206223 205687 −1 537 P3.orf0193 D-lactate dehydrogenase 206420 207871 21452 P3.orf0194 ssuA Putative aliphatic sulfonates-binding protein208092 209174 3 1083 P3.orf0195 ABC transporter ATP-binding protein209185 209952 1 768 P3.orf0196 tauC binding-protein-dependent transportsystems 209945 210766 2 822 inner membrane component P3.orf0197 zntBMg2+ transporter protein, CorA-like 210794 211834 2 1041 P3.orf0198transcriptional regulator, GntR family with 214073 212691 −3 1383aminotransferase domain P3.orf0199 conserved hypothetical protein 214491214973 3 483 P3.orf0200 nitA putative amidohydrolase 215030 216106 21077 P3.orf0201 Radical SAM domain protein 216189 217202 3 1014P3.orf0202 putative histone acetyltransferase 217199 217756 2 558P3.orf0203 selenophosphate synthetase-related protein 217753 218772 11020 P3.orf0204 glycosyl transferase group 1 218769 219959 3 1191P3.orf0205 conserved hypothetical protein 219956 220243 2 288 P3.orf0206putative flavoprotein involved in K+ transport 220249 221529 1 1281P3.orf0207 conserved hypothetical protein 221858 221541 −3 318P3.orf0208 putative oxidoreductase protein 222622 221873 −2 750P3.orf0209 ycaN transcriptional regulator, LysR family 223647 222733 −1915 P3.orf0210 enoyl-CoA hydratase 223743 224489 3 747 P3.orf0211 Hadh3-hydroxyacyl-CoA dehydrogenase 224636 225592 2 957 P3.orf0212 ybfItranscriptional activator 226298 226915 2 618 P3.orf0213 pamO steroidmonooxygenase 227173 228822 1 1650 P3.orf0214 metX esterase 228849229904 3 1056 P3.orf0215 conserved hypothetical protein 230906 230031 −3876 P3.orf0216 TetR family transcriptional regulator 231603 230980 −1624 P3.orf0217 ybhR multidrug ABC transporter permease 232801 231680 −21122 component P3.orf0218 ybhS multidrug ABC transporter permease 233368232805 −2 564 component P3.orf0219 ybhS putative ABC transporterpermease protein 233931 233365 −1 567 P3.orf0220 ybhF ABC transporterrelated protein 235682 233928 −3 1755 P3.orf0221 membrane protein 236706235687 −1 1020 P3.orf0222 ybiH TetR family transcriptional regulator237326 236703 −3 624 P3.orf0223 conserved hypothetical protein 238615237500 −2 1116 P3.orf0224 paaA phenylacetic acid degradation protein(similar 239551 238799 −2 753 to paaA) P3.orf0225 hypothetical protein240236 239757 −3 480 P3.orf0226 Radical SAM domain protein 241498 240233−2 1266 P3.orf0227 hcaE Rieske (2Fe—2S) domain-containing protein 242879241698 −3 1182 P3.orf0228 thioesterase superfamily protein 243659 243189−3 471 P3.orf0229 ydeM MaoC-like dehydratase 244141 243656 −2 486P3.orf0230 ligA NAD-dependent DNA ligase 246344 244233 −3 2112P3.orf0231 recN DNA repair protein RecN 248114 246438 −3 1677 P3.orf0232DNA uptake lipoprotein 249069 248191 −1 879 P3.orf0233 DNA-bindingprotein 249926 250372 2 447 P3.orf0234 lpxC UDP-3-O-[3-hydroxymyristoyl]N- 251433 250468 −1 966 acetylglucosamine deacetylase P3.orf0235 ftsZcell division protein FtsZ 253351 251786 −2 1566 P3.orf0236 ftsAF492457_3 cell division protein FtsA 254667 253432 −1 1236 P3.orf0237ftsQ Cell division protein FtsQ 255726 254782 −1 945 P3.orf0238 ddlD-alanine-D--alanine ligase 256628 255714 −3 915 P3.orf0239 murBUDP-N-acetylenolpyruvoylglucosamine 257635 256625 −2 1011 reductaseP3.orf0240 murC UDP-N-acetylmuramate--L-alanine ligase 259059 257635 −11425 P3.orf0241 murG UDP-N-acetylglucosamine-N-acetylmuramyl- 260279259056 −3 1224 (pentapeptide) pyrophosphoryl-undecaprenolN-acetylglucosamine transferase P3.orf0242 ftsW putative cell divisionprotein ftsW 261397 260276 −2 1122 P3.orf0243 murDUDP-N-acetylmuramoyl-L-alanyl-D-glutamate 262860 261394 −1 1467synthetase P3.orf0244 mraY Glycosyl transferase, family 4 263966 262878−3 1089 P3.orf0245 murF UDP-N-acetylmuramoyl-tripeptide--D-alanyl-D-265477 264020 −2 1458 alanine ligase subfamily P3.orf0246 murEUDP-N-acetylmuramoylalanyl-D-glutamate-- 266946 265474 −1 14732,6-diaminopimelate ligase P3.orf0247 penA peptidoglycan synthetase FtsI268828 266933 −2 1896 P3.orf0248 periplasmic protein 269292 268825 −1468 P3.orf0249 mraW S-adenosyl-methyltransferase mraW 270293 269289 −31005 P3.orf0250 mraZ cell division protein MraZ 270811 270290 −2 522P3.orf0251 ampD negative regulator of AmpC, AmpD 272679 271888 −1 792P3.orf0252 eamA integral membrane protein 273634 272702 −2 933P3.orf0253 hfaC ABC transporter related protein 275499 273631 −1 1869P3.orf0254 curculin domain-containing protein 276723 275686 −1 1038P3.orf0255 djlA DnaJ-like protein DjlA 277594 276848 −2 747 P3.orf0256bacterial extracellular solute-binding protein, 277977 278957 3 981family 7 P3.orf0257 tripartite ATP-independent periplasmic 279099 2796563 558 transporter, DctQ component P3.orf0258 TRAP dicarboxylatetransporter, DctM subunit 279676 280986 1 1311 P3.orf0259 OXP15-oxoprolinase (ATP-hydrolyzing) 281063 284704 2 3642 P3.orf0260conserved hypothetical protein 284715 285302 3 588 P3.orf0261 conservedhypothetical protein 285447 285947 3 501 P3.orf0262 conservedhypothetical protein 285944 287074 2 1131 P3.orf0263 conservedhypothetical protein 287088 287303 3 216 P3.orf0264 yneJ putativetranscriptional regulator protein, LysR 287445 288308 3 864 familyP3.orf0265 putative transmembrane protein 288326 289105 2 780 P3.orf0266pyruvate, phosphate dikinase 289409 291367 2 1959 P3.orf0267 ydcN XREfamily transcriptional regulator 292040 291459 −3 582 P3.orf0268Gluconate 2-dehydrogenase subunit 3 292191 292955 3 765 P3.orf0269Gluconate 2-dehydrogenase (acceptor) 293016 294779 3 1764 P3.orf0270diheme cytochrome c SoxE 294776 295147 2 372 P3.orf0271 ykpBKetopantoate reductase 295209 296135 3 927 P3.orf0272 ydeK transporterydeK 296132 296998 2 867 P3.orf0273 hxlR transcriptional regulator297307 297005 −2 303 P3.orf0274 gstB glutathione S-transferase 297503298117 2 615 P3.orf0275 conserved hypothetical protein 298114 298491 1378 P3.orf0276 4-oxalocrotonate tautomerase family protein 298504 2987101 207 P3.orf0277 thcD FAD-dependent pyridine nucleotide-disulphide300150 298870 −1 1281 oxidoreductase P3.orf0278 hrb rubredoxin reductase300460 300251 −2 210 P3.orf0279 conserved hypothetical protein 301498300509 −2 990 P3.orf0280 glyA glycine hydroxymethyltransferase 303294302002 −1 1293 P3.orf0281 yfeJ glutamine amidotransferase class-I 304426303725 −2 702 P3.orf0282 ptsJ GntR family transcriptional regulator304715 306028 2 1314 P3.orf0283 cupin 2 domain-containing protein 306617306231 −3 387 P3.orf0284 yedZ Ferric reductase domain protein protein306862 307848 1 987 transmembrane component domain protein P3.orf0285ydeR Major facilitator superfamily MFS_1 308978 307812 −3 1167P3.orf0286 ycaN LysR family transcriptional regulator 310241 311167 2927 P3.orf0287 yajO aldo/keto reductase 310081 309035 −2 1047 P3.orf0288menE citrate synthase 311990 313657 2 1668 P3.orf0289 putativeglutathione S-transferase-related 313654 314640 1 987 protein P3.orf0290short-chain dehydrogenase/reductase SDR 314637 315410 3 774 P3.orf0291creA CreA family protein 315498 315989 3 492 P3.orf0292 LemA familyprotein 316110 316691 3 582 P3.orf0293 htpX HtpX-2 peptidase 316696317859 1 1164 P3.orf0294 virR conserved hypothetical protein 318375317875 −1 501 P3.orf0295 fixR short-chain dehydrogenase 318540 319295 3756 P3.orf0296 hypothetical protein 320005 320121 1 117 P3.orf0297 yjfLinner membrane protein YjfL 320245 320667 1 423 P3.orf0298 conservedhypothetical protein 320745 321326 3 582 P3.orf0299 ygiCglutathionylspermidine synthase 321341 322552 2 1212 P3.orf0300conserved hypothetical protein 323212 322577 −2 636 P3.orf0301 cqsSCAI-1 autoinducer sensor kinase/phosphatase 324242 323349 −3 894 cqsSP3.orf0302 pleC response regulator receiver sensor signal 325762 324248−2 1515 transduction histidine kinase P3.orf0303 iorA indolepyruvateferredoxin oxidoreductase, 329497 325985 −2 3513 alpha subunitP3.orf0304 putative TetR family protein receptor protein 330225 329839−1 387 P3.orf0305 conserved hypothetical protein 330266 330535 2 270P3.orf0306 conserved hypothetical protein 330489 330956 3 468 P3.orf0307zraR two component, sigma54 specific, Fis family 332800 331025 −2 1776transcriptional regulator P3.orf0308 yjbG Oligoendopeptidase F homolog332996 334807 2 1812 P3.orf0309 aarF domain-containing kinase 334862336265 2 1404 P3.orf0310 hypothetical protein 336546 337256 3 711P3.orf0311 diguanylate cyclase/phosphodiesterase 339938 337284 −3 2655P3.orf0312 hipO amidohydrolase 341077 339941 −2 1137 P3.orf0313hypothetical protein 341375 341542 2 168 P3.orf0314 ilvG thiaminepyrophosphate protein central region 341626 343383 1 1758 P3.orf0315conserved hypothetical protein 343422 345497 3 2076 P3.orf0316 conservedhypothetical protein 345494 347092 2 1599 P3.orf0317 conservedhypothetical protein 347500 347114 −2 387 P3.orf0318 conservedhypothetical protein 347766 347518 −1 249 P3.orf0319 ttuDhydroxypyruvate reductase 349150 347876 −2 1275 P3.orf0320 Creatininase349350 350093 3 744 P3.orf0321 N-formylglutamate amidohydrolase 350106350867 3 762 P3.orf0322 conserved hypothetical protein 350920 351222 1303 P3.orf0323 ytcJ amidohydrolase 3 353140 351260 −2 1881 P3.orf0324conserved hypothetical protein 353483 353193 −3 291 P3.orf0325 ybfItranscriptional regulator, AraC family 353634 354530 3 897 P3.orf0326conserved hypothetical protein 354659 354910 2 252 P3.orf0327 conservedhypothetical protein 355041 355385 3 345 P3.orf0328 hypothetical protein355851 355375 −1 477 P3.orf0329 conserved hypothetical protein 356637355870 −1 768 P3.orf0330 conserved hypothetical protein 357494 356634 −3861 P3.orf0331 conserved hypothetical protein 357888 357541 −1 348P3.orf0332 cqsA CAI-1 autoinducer synthase 358328 359608 2 1281P3.orf0333 SRY1 Threo-3-hydroxyaspartate ammonia-lyase 360652 359672 −2981 P3.orf0334 ydfD transcriptional regulator, GntR family with 360821362302 2 1482 aminotransferase domain P3.orf0335 L-lactate permease362445 364148 3 1704 P3.orf0336 transcriptional regulator 364222 3645601 339 P3.orf0337 arsB arsenical-resistance protein 364569 365630 3 1062P3.orf0338 NADPH-dependent FMN reductase 365627 366391 2 765 P3.orf0339ggt gamma-glutamyltranspeptidase 366507 368312 3 1806 P3.orf0340 epoxidehydrolase protein 368348 369241 2 894 P3.orf0341 AraC familytranscriptional regulator 369241 370308 1 1068 P3.orf0342 putativeregulator PrlF 370305 370499 3 195 P3.orf0343 conserved hypotheticalprotein 371729 370581 −3 1149 P3.orf0344 conserved hypothetical protein372148 371726 −2 423 P3.orf0345 conserved hypothetical protein 372478373455 1 978 P3.orf0346 ddpA ABC transporter substrate-binding protein373624 375249 1 1626 P3.orf0347 dppB ABC transporter permease protein 2375326 376348 2 1023 P3.orf0348 ddpC ABC transporter permease protein 1376345 377193 1 849 P3.orf0349 gsiA ABC transporter ATP-binding protein377190 378911 3 1722 P3.orf0350 mcbR Transcriptional Regulator, GntRfamily protein 378980 379708 2 729 P3.orf0351 aminotransferase class-III379990 381345 1 1356 P3.orf0352 hydantoin utilization protein 381360383396 3 2037 P3.orf0353 putative N-methylhydantoinase B 383401 385014 11614 P3.orf0354 ygaY major facilitator transporter 386318 385119 −3 1200P3.orf0355 HxlR family transcriptional regulator 386421 386861 3 441P3.orf0356 GntR family transcriptional regulator 387573 386902 −1 672P3.orf0357 bcpA isocitrate lyase and phosphorylmutase 387704 388567 2864 P3.orf0358 isopropylmalate isomerase large subunit 388564 389985 11422 P3.orf0359 leuD isopropylmalate isomerase small subunit 389985390620 3 636 P3.orf0360 yraM conserved hypothetical protein 390701391888 2 1188 P3.orf0361 dctP c4-dicarboxylate-binding periplasmicprotein 391939 392961 1 1023 P3.orf0362 TRAP-type C4-dicarboxylatetransport system 392966 393532 2 567 small permease P3.orf0363C4-dicarboxylate TRAP-T family tripartite ATP- 393529 394812 1 1284independent periplasmic transporter, membrane protein, large subunitP3.orf0364 citH proline/glycine betaine transporter, Major 394995 3962873 1293 facilitator superfamily P3.orf0365 mqsR Motility quorum-sensingregulator mqsR 396479 396775 2 297 P3.orf0366 ygiT transcriptionalregulator, XRE family 396777 397178 3 402 P3.orf0367 hypotheticalprotein 397787 398224 2 438 P3.orf0368 lcfA long-chain-fatty-acid--CoAligase 400068 398320 −1 1749 P3.orf0369 ygfF short-chaindehydrogenase/reductase SDR 400154 400912 2 759 P3.orf0370 bioCmethyltransferase 400934 401659 2 726 P3.orf0371 F377340_3 AraC 402662401670 −3 993 P3.orf0372 dienelactone hydrolase family protein 402712403524 1 813 P3.orf0373 conserved hypothetical protein 403624 404664 11041 P3.orf0374 pleC sensory transduction histidine kinase 406997 404673−3 2325 P3.orf0375 dht dihydropyrimidinase 408628 407168 −2 1461P3.orf0376 pucI cytosine/purines, uracil, thiamine, allantoin 410219408690 −3 1530 transporter P3.orf0377 yeiA dihydroorotate dehydrogenasefamily protein 411740 410436 −3 1305 P3.orf0378 yeiT putativeoxidoreductase 413141 411768 −3 1374 P3.orf0379 hyuC amidase,hydantoinase/carbamoylase family 414563 413307 −3 1257 P3.orf0380 mmsAmethylmalonate-semialdehyde dehydrogenase 416162 414663 −3 1500P3.orf0381 rutR transcriptional regulator, TetR family protein 416868417575 3 708 P3.orf0382 suhB putative inositol monophosphatase 417572418438 2 867 P3.orf0383 CRISPR-associated protein Cas2 418894 418604 −2291 P3.orf0384 conserved hypothetical protein 420031 418940 −2 1092P3.orf0385 putative crispr-associated protein cas4 420690 420028 −1 663P3.orf0386 CRISPR-associated Csh2 family protein 421602 420730 −1 873P3.orf0387 CRISPR-associated protein, Csd1 family 423567 421660 −1 1908P3.orf0388 CRISPR-associated protein Cas5 family 424163 423564 −3 600P3.orf0389 helicase 426840 424429 −1 2412 P3.orf0390 conservedhypothetical protein 427477 427037 −2 441 P3.orf0391 Pirin domainprotein 428530 427601 −2 930 P3.orf0392 ybfI transcriptional regulator,AraC family 429531 428647 −1 885 P3.orf0393 mdeA Cystathioninegamma-synthase 429596 430801 2 1206 P3.orf0394 ydeL transcriptionalregulator, GntR family with 432223 430811 −2 1413 aminotransferasedomain protein P3.orf0395 FMN-binding negative transcriptional regulator432301 432936 1 636 P3.orf0396 putative nitronate monooxygenase 434092433112 −2 981 P3.orf0397 HxlR family transcriptional regulator 434310434867 3 558 P3.orf0398 conserved hypothetical protein 435492 434926 −1567 P3.orf0399 D-isomer specific 2-hydroxyacid 435713 436675 2 963dehydrogenase NAD-binding P3.orf0400 hypothetical protein 438321 436699−1 1623 P3.orf0401 2OG-Fe(II) oxygenase family protein 438775 439785 11011 P3.orf0402 hypothetical protein 438564 438355 −1 210 P3.orf0403Glyoxalase/bleomycin resistance 439832 440221 2 390 protein/dioxygenaseP3.orf0404 med Bmp family membrane protein 440300 441394 2 1095P3.orf0405 yufO ABC transporter related protein 441436 442977 1 1542P3.orf0406 yufP inner-membrane translocator 442970 444100 2 1131P3.orf0407 yufQ branched chain amino acid ABC transporter, 444097 4450291 933 permease protein P3.orf0408 amn AMP nucleosidase 445120 446628 11509 P3.orf0409 hypothetical protein 446711 447028 2 318 P3.orf0410 HicBfamily protein 447193 447432 1 240 P3.orf0411 conserved hypotheticalprotein 447502 448788 1 1287 P3.orf0412 SupD 448785 449744 3 960P3.orf0413 yakc aldo/keto reductase 449848 450882 1 1035 P3.orf0414 yhgDTetR family transcriptional regulator 450910 451506 1 597 P3.orf0415AraC family transcriptional regulator 451595 452563 2 969 P3.orf0416major facilitator transporter 452652 453845 3 1194 P3.orf0417 fyuATonB-dependent receptor 453931 456051 1 2121 P3.orf0418O-methyltransferase family protein 456051 457061 3 1011 P3.orf0419 yafCtranscriptional regulator protein 457981 457088 −2 894 P3.orf0420 stptransporter protein 458114 459625 2 1512 P3.orf0421 NQO1 NAD(P)H quinoneoxidoreductase 460322 459654 −3 669 P3.orf0422 nahR LysR substratebinding domain protein 460421 461374 2 954 P3.orf0423 yebE Innermembrane protein yebE 462176 461334 −3 843 P3.orf0424 acetyl-CoAcarboxylase 462381 463019 3 639 P3.orf0425 yjbB Na/Pi-cotransporterII-related protein 464741 463029 −3 1713 P3.orf0426 hypothetical protein465315 464797 −1 519 P3.orf0427 hypothetical protein 465715 465299 −2417 P3.orf0428 NADH-ubiquinone oxidoreductase 466049 465705 −3 345P3.orf0429 PED1 Acetyl-CoA C-acyltransferase 467453 466269 −3 1185P3.orf0430 braG putative high-affinity branched-chain amino 468382467660 −2 723 acid transport ATP-binding proteinputative P3.orf0431 braFABC superfamily ATP binding cassette 469152 468379 −1 774 transporter,ABC protein P3.orf0432 branched-chain amino acid ABC transporter 470993469149 −3 1845 P3.orf0433 putative branched-chain amino acid ABC 472322471105 −3 1218 transporter, periplasmic substrate-binding proteinP3.orf0434 dehydrogenase 473394 472480 −1 915 P3.orf0435 rsmAMethyltransferase type 12 474041 473445 −3 597 P3.orf0436 iorB Aldehydeoxidase and xanthine 476400 474082 −1 2319 dehydrogenase, molybdopterinbinding protein P3.orf0437 iorA Membrane-bound aldehyde dehydrogenase476874 476410 −1 465 iron-sulfur protein P3.orf0438 yqhC transcriptionalregulator protein 477114 478010 3 897 P3.orf0439 apl putative alkalinephosphatase protein 478111 478704 1 594 P3.orf0440 sbcD nuclease SbcCD,D subunit 478813 480060 1 1248 P3.orf0441 sbcC exonuclease SbcC 480060483848 3 3789 P3.orf0442 aminotransferase 485304 483934 −1 1371P3.orf0443 ydeL transcriptional regulator, GntR family 485526 486989 31464 P3.orf0444 mocR transcriptional regulator 488515 487031 −2 1485P3.orf0445 bam Indoleacetamide hydrolase 488833 490233 1 1401 P3.orf0446ytcJ amidohydrolase-like 490463 492109 2 1647 P3.orf0447 cobalaminsynthesis protein, P47K 492109 493038 1 930 P3.orf0448 ALD4 aldehydedehydrogenase 5 493082 494605 2 1524 P3.orf0449 conserved hypotheticalprotein 494617 495354 1 738 P3.orf0450 appA extracellular solute-bindingprotein family 5 495476 497071 2 1596 P3.orf0451 dppB binding-proteindependent transport system 497178 498197 3 1020 inner membrane proteinP3.orf0452 appC binding-protein-dependent transport systems 498194499108 2 915 inner membrane component P3.orf0453 oligopeptide/dipeptideABC transporter, 499108 500118 1 1011 ATPase subunit P3.orf0454 appFoligopeptide/dipeptide ABC transporter, 500115 501104 3 990 ATPasesubunit P3.orf0455 acdS 1-aminocyclopropane-1-carboxylate 502175 501159−3 1017 deaminase P3.orf0456 lrp leucine-responsive regulatory protein502373 502843 2 471 P3.orf0457 NADH dehydrogenase protein 504282 502864−1 1419 P3.orf0458 putative addiction module antidote protein, 505113505406 3 294 CopG/Arc/MetJ family P3.orf0459 conserved hypotheticalprotein 505091 504606 −3 486 P3.orf0460 yoaH chemotaxis sensorytransducer 506986 505490 −2 1497 P3.orf0461 glnQ polar amino acidtransport system ATP- 507949 507197 −2 753 binding protein P3.orf0462polar amino acid ABC transporter, inner 508586 507936 −3 651 membranesubunit P3.orf0463 yecS glutamine ABC superfamily ATP binding 509304508579 −1 726 cassette transporter, membrane protein P3.orf0464 glnHamino acid ABC transporter substrate-binding 510214 509390 −2 825protein P3.orf0465 lutR GntR domain-containing protein 510487 511170 1684 P3.orf0466 alc1 allantoicase 511212 512225 3 1014 P3.orf0467 allAureidoglycolate hydrolase 512234 512761 2 528 P3.orf0468 hypotheticalprotein 514441 512849 −2 1593 P3.orf0469 hypothetical protein 514877515026 2 150 P3.orf0470 hypothetical protein 514651 514505 −2 147P3.orf0471 nrdZ putative B12-dependent ribonucleoside- 516753 515536 −11218 diphosphate/-triphosphate reductase (nrdJ- like) P3.orf0472hypothetical protein 517886 518038 2 153 P3.orf0473 ydcR Bifunctional --Transcriptional Regulator, GntR 519443 518043 −3 1401family/Aminotransferase, class I and II P3.orf0474 MFS transporter519730 521019 1 1290 P3.orf0475 transcriptional regulator, BadM/Rrf2family 521021 521401 2 381 P3.orf0476 HPr kinase 521401 522249 1 849P3.orf0477 sulfotransferase 522246 523112 3 867 P3.orf0478 glcFGlycolate oxidase iron-sulfur subunit 525337 523952 −2 1386 P3.orf0479glcE glycolate oxidase, subunit glcE 526566 525334 −1 1233 P3.orf0480glcD FAD linked oxidase-like protein 528058 526571 −2 1488 P3.orf0481rpmJ 50S ribosomal protein L36 528391 528266 −2 126 P3.orf0482hypothetical protein 528619 529317 1 699 P3.orf0483 Putativephosphoethanolamine N- 530285 529314 −3 972 methyltransferase P3.orf0484lcfA acyl-CoA synthetase 532444 530696 −2 1749 P3.orf0485 biotindependent acyl-CoA carboxylase 532599 534194 3 1596 P3.orf0486 des fattyacid desaturase 534570 535637 3 1068 P3.orf0487 conserved hypotheticalprotein 536061 535885 −1 177 P3.orf0488 XRE family transcriptionalregulator 537253 536912 −2 342 P3.orf0489 hypothetical protein 537835537951 1 117 P3.orf0490 fad dependent oxidoreductase; protein 539165537972 −3 1194 P3.orf0491 valyl-tRNA synthetase 541083 539137 −1 1947P3.orf0492 betB Aldehyde Dehydrogenase 542856 541297 −1 1560 P3.orf04936-phosphogluconate dehydrogenase NAD- 543742 542873 −2 870 bindingP3.orf0494 cynR LysR family transcriptional regulator 543784 544932 11149 P3.orf0495 acetyltransferase (GNAT) family protein 545532 545140 −1393 P3.orf0496 conserved hypothetical protein 545943 545662 −1 282P3.orf0497 ABC transporter related protein 546858 546079 −1 780P3.orf0498 binding-protein dependent transport system 547711 546917 −2795 inner membrane protein P3.orf0499 ssuA putative sulfonate/nitratetransport system 548718 547735 −1 984 substrate-binding proteinP3.orf0500 agmatinase 549766 548759 −2 1008 P3.orf0501 cmpR putativeLysR-family regulatory protein 549871 550782 1 912 P3.orf0502 putativemembrane protein 550993 551823 1 831 P3.orf0503 conserved hypotheticalprotein 551829 552332 3 504 P3.orf0504 sdaA L-serine dehydratase 1552433 553821 1 1389 P3.orf0505 ytnL putative hydrolase 553879 555078 11200 P3.orf0506 dgdR transcriptional regulator, LysR family 555974555093 −3 882 P3.orf0507 icd isocitrate dehydrogenase 556111 557196 11086 P3.orf0508 conserved hypothetical protein 557266 557913 1 648P3.orf0509 methyl-accepting chemotaxis protein 559595 557919 −3 1677P3.orf0510 methyl-accepting chemotaxis protein 561525 559840 −1 1686P3.orf0511 mcp3 methyl-accepting chemotaxis sensory 563423 561738 −31686 transducer P3.orf0512 anti-sigma factor, ChrR 563646 564329 3 684P3.orf0513 yhbS GCN5-related N-acetyltransferase 564359 564871 2 513P3.orf0514 Aass Alpha-aminoadipic semialdehyde synthase 565472 566539 21068 P3.orf0515 putative transcriptional regulator, AsnC family 565324564887 −2 438 protein P3.orf0516 CRISPR-associated protein Cas2 567419567090 −3 330 P3.orf0517 conserved hypothetical protein 568244 567429 −3816 P3.orf0518 conserved hypothetical protein 571430 568281 −3 3150P3.orf0519 hypothetical protein 571876 571736 −2 141 P3.orf0520 putativeABC transporter-binding protein 572058 573365 3 1308 P3.orf0521binding-protein-dependent transport systems 573362 574309 2 948 innermembrane component P3.orf0522 yurM maltose/maltodextrin ABC transporter574306 575139 1 834 permease protein MalG P3.orf0523 smoK maltose 575149576219 1 1071 P3.orf0524 rpfC GAF sensor hybrid histidine kinase 576303578552 3 2250 P3.orf0525 gstA conserved hypothetical protein 578676579287 3 612 P3.orf0526 dctA sodium: dicarboxylate symporter 580834579419 −2 1416 P3.orf0527 dctB histidine kinase 581119 583020 1 1902P3.orf0528 dctD C4-dicarboxylate transport transcriptional 583017 5843963 1380 regulatory protein dctD P3.orf0529 MGLL hydrolase 585291 584416−1 876 P3.orf0530 ydaM diguanylate cyclase 587186 585414 −3 1773P3.orf0531 tnaA tryptophanase 587561 589075 2 1515 P3.orf0532 conservedhypothetical protein 589207 589812 1 606 P3.orf0533 sodB superoxidedismutase 589927 590535 1 609 P3.orf0534 trpI LysR familytranscriptional regulator 591413 590499 −3 915 P3.orf0535 pdxA4-hydroxythreonine-4-phosphate 591591 592613 3 1023 dehydrogenaseP3.orf0536 ydbB Cupin domain protein 592618 593676 1 1059 P3.orf0537putative oxidoreductase protein 594448 593699 −2 750 P3.orf0538 yhjCtranscriptional regulator protein 594536 595471 2 936 P3.orf0539transporter 596621 595425 −3 1197 P3.orf0540 pat Phosphinothricinacetyltransferase 597202 596618 −2 585 P3.orf0541 cmpR LysR familytranscriptional regulator 597267 598265 3 999 P3.orf0542 ywnBRrf2-linked NADH-flavin reductase 599486 598875 −3 612 P3.orf0543 ytfHHTH-type transcriptional regulator ytfH 599657 600055 2 399 P3.orf0544TetR family transcriptional regulator 600669 600043 −1 627 P3.orf0545putative NAD(P)H dehydrogenase 600774 601382 3 609 P3.orf0546 cyclasefamily protein 601466 602404 2 939 P3.orf0547 tpm thiopurineS-methyltransferase 603073 602429 −2 645 P3.orf0548 moaR transcriptionalregulator, LuxR family 603210 603926 3 717 P3.orf0549 amine oxidase605247 603910 −1 1338 P3.orf0550 short-chain dehydrogenase/reductasefamily 606076 605336 −2 741 oxidoreductase P3.orf0551 AraC familytranscriptional regulator 606190 607110 1 921 P3.orf0552 majorfacilitator superfamily MFS_1 607197 608450 3 1254 P3.orf0553 tdhATonB-dependent heme/hemoglobin receptor 610963 608438 −2 2526 familyprotein P3.orf0554 FecR protein 611984 611058 −3 927 P3.orf0555FecI-like protein 612481 611981 −2 501 P3.orf0556 ycdO conservedhypothetical protein 613577 612753 −3 825 P3.orf0557 ycdB Dyp-typeperoxidase family protein 614927 613623 −3 1305 P3.orf0558 ycdOconserved hypothetical protein 616107 614929 −1 1179 P3.orf0559 efeUiron permease FTR1 616954 616109 −2 846 P3.orf0560 conservedhypothetical protein 623392 623982 1 591 P3.orf0561 pleC sensorhistidine kinase (non-motile and phage- 624021 625847 3 1827 resistanceprotein) P3.orf0562 pleC Signal transduction histidine kinase 627293625860 −3 1434 P3.orf0563 purU formyltetrahydrofolate deformylase 627659628516 2 858 P3.orf0564 TRAP transporter solute receptor TAXI family628659 629678 3 1020 protein P3.orf0565 TRAP transporter, 4TM/12TMfusion protein 629779 631911 1 2133 P3.orf0566 conserved hypotheticalprotein 631908 632378 3 471 P3.orf0567 membrane protein-like protein632378 633676 2 1299 P3.orf0568 cupin 2 domain-containing protein 633719634216 2 498 P3.orf0569 fabG short-chain dehydrogenase/reductase SDR634978 634232 −2 747 P3.orf0570 hypothetical protein 635089 635322 1 234P3.orf0571 luxQ two-component hybrid sensor and regulator 635442 6389243 3483 P3.orf0572 agmR two-component response regulator 639596 638943 −3654 P3.orf0573 proP major facilitator superfamily 641507 639789 −3 1719P3.orf0574 putative hydrolase/carboxylic esterase 642770 641817 −3 954P3.orf0575 Leu/Ile/Val-binding protein 644023 642791 −2 1233 P3.orf0576mexR MarR family transcriptional regulator 644239 644751 1 513P3.orf0577 conserved hypothetical protein 644833 645165 1 333 P3.orf0578conserved hypothetical protein 645504 645773 3 270 P3.orf0579transcriptional regulator, XRE family 645764 646111 2 348 P3.orf0580yvoA transcriptional regulator, GntR family 647380 648111 1 732P3.orf0581 amidohydrolase 2 647292 646474 −1 819 P3.orf0582 conservedhypothetical protein 648226 649182 1 957 P3.orf0583 conservedhypothetical protein 649184 649621 2 438 P3.orf0584 integral membraneprotein 649637 651130 2 1494 P3.orf0585 conserved hypothetical protein651713 651135 −3 579 P3.orf0586 mnmA tRNA(5-methylaminomethyl-2-thiouridylate)- 653006 651852 −3 1155methyltransferase P3.orf0587 Transcriptional activator, TenA familyprotein 653783 653061 −3 723 P3.orf0588 fdxB 2Fe—2S ferredoxin 654279653947 −1 333 P3.orf0589 Iron-sulfur assembly protein 654862 654530 −2333 P3.orf0590 mdtA secretion protein 655170 656342 3 1173 P3.orf0591hydrophobic/amphiphilic exporter-1 656364 659462 3 3099 P3.orf0592 ybaLpotassium efflux system protein 661960 660227 −2 1734 P3.orf0593hypothetical protein 662198 662629 2 432 P3.orf0594 secD protein-exportmembrane protein SECD 662767 665103 1 2337 P3.orf0595 grsT Type IIthioesterase 665186 665971 2 786 P3.orf0596 phbA Membrane proteasesubunit 667426 665975 −2 1452 stomatin/prohibitin-like proteinP3.orf0597 yojI Cyclic peptide transporter 669105 667423 −1 1683P3.orf0598 hypothetical protein 669427 669215 −2 213 P3.orf0599 GTPasedomain-containing protein 670123 669551 −2 573 P3.orf0600cation/multidrug efflux pump protein 673337 670374 −3 2964 P3.orf0601putative HlyD-like secretion protein 674600 673467 −3 1134 P3.orf0602lgrC OciB protein 674941 681312 1 6372 P3.orf0603 pksJ linear gramicidinsynthetase subunit D 681309 686699 3 5391 P3.orf0604 lgrC non-ribosomalpeptide synthetase 686696 690688 2 3993 P3.orf0605 lgrC putativenonribosomal peptide synthetases 690664 702225 1 11562 (NPRS) P3.orf0606lgrC erythronolide synthase 702250 707367 1 5118 P3.orf0607 pksJ HctF707383 712224 1 4842 P3.orf0608 pksJ non-ribosomal peptidesynthetase/polyketide 712221 716462 3 4242 synthase P3.orf0609 lgrCnon-ribosomal peptide synthetase 716459 720319 2 3861 P3.orf0610 lgrCnon-ribosomal peptide synthetase 720291 722912 3 2622 P3.orf0611 lgrCpeptide synthetase 722909 729400 2 6492 P3.orf0612 mbtH MbtHdomain-containing protein 729464 729697 2 234 P3.orf0613 conservedhypothetical protein 731518 731312 −2 207 P3.orf0614 hypotheticalprotein 732225 732362 3 138 P3.orf0615 hypothetical protein 732366732548 3 183 P3.orf0616 conserved hypothetical protein 733333 732761 −2573 P3.orf0617 conserved hypothetical protein 734754 734527 −1 228P3.orf0618 yyaK CAAX amino terminal protease family 736217 735243 −3 975P3.orf0619 yycB cyanate transport system protein 737535 736339 −1 1197P3.orf0620 transcriptional regulator, GntR family 738299 737532 −3 768P3.orf0621 putative SURF1 family protein 739095 738403 −1 693 P3.orf0622cyoD cytochrome o ubiquinol oxidase subunit IV 739619 739194 −3 426P3.orf0623 cyoC cytochrome o ubiquinol oxidase subunit III 740278 739616−2 663 P3.orf0624 cyoB cytochrome o ubiquinol oxidase, subunit I 742286740283 −3 2004 (ubiquinol oxidase chain A) P3.orf0625 cyoA ubiquinoloxidase, subunit II 743480 742305 −3 1176 P3.orf0626 type III effectorprotein 744090 744644 3 555 P3.orf0627 hypothetical protein 744655745092 1 438 P3.orf0628 DNA methylase N-4/N-6 745248 748043 3 2796P3.orf0629 type III restriction enzyme, res subunit 748063 751170 1 3108P3.orf0630 UvrD/REP type DNA helicase 753318 751198 −1 2121 P3.orf0631pdxJ pyridoxal phosphate biosynthetic protein PdxJ 754149 753397 −1 753P3.orf0632 hypothetical protein 754142 754318 2 177 P3.orf0633 ggpSalpha,alpha-trehalose-phosphate synthase 755880 754360 −1 1521P3.orf0634 ggpS alpha,alpha-trehalose-phosphate synthase 756681 755959−1 723 P3.orf0635 gpsA glycerol-3-phosphate dehydrogenase 757862 756681−3 1182 P3.orf0636 aglA putative alpha-glucosidase AglA 758072 759703 21632 P3.orf0637 glutathione-dependent formaldehyde- 759738 760196 3 459activating GFA P3.orf0638 TetR family transcriptional regulator 760355760999 2 645 P3.orf0639 ilvE aminotransferase, class IV 761003 761953 2951 P3.orf0640 conserved hypothetical protein 761953 763626 1 1674P3.orf0641 crt 3-hydroxybutyryl-CoA dehydratase 764644 763850 −2 795P3.orf0642 oruR AraC family transcriptional regulator 764810 765886 21077 P3.orf0643 FAD/FMN-containing dehydrogenases 767279 765852 −3 1428P3.orf0644 lutR transcriptional regulator, GntR 767533 769188 1 1656family/amidohydrolase family protein P3.orf0645 yiaO putativeperiplasmic substrate-binding 769391 770386 2 996 transport proteinP3.orf0646 siaT TRAP-type C4-dicarboxylate transport system, 770492770998 2 507 small permease component P3.orf0647 ygiK C4-dicarboxylatetransport system (permease 770995 772278 1 1284 large protein)P3.orf0648 cysB cystathionine beta-synthase 772491 773513 3 1023P3.orf0649 2-isopropylmalate synthase 773510 774922 2 1413 P3.orf0650conserved hypothetical protein 774877 775938 1 1062 P3.orf0651 conservedhypothetical protein 776020 777183 1 1164 P3.orf0652 Argininosuccinatelyase 2 777193 778479 1 1287 P3.orf0653 conserved hypothetical protein778476 779720 3 1245 P3.orf0654 hypothetical protein 779737 781011 11275 P3.orf0655 short chain dehydrogenase 782717 781806 −3 912P3.orf0656 transcriptional regulator, TetR family 782822 783403 2 582P3.orf0657 iclR IclR family transcriptional regulator 784283 783417 −3867 P3.orf0658 gentisate 1,2-dioxygenase 784491 785555 3 1065 P3.orf0659fumarylacetoacetat hydroxylase 785552 786268 2 717 P3.orf0660 siaP TRAPdicarboxylate transporter- DctP subunit 786340 787383 1 1044 P3.orf0661hypothetical protein 787380 787946 3 567 P3.orf0662 siaT trapdicarboxylate transporter, dctm subunit 787953 789257 3 1305 P3.orf0663maiA maleylacetoacetate isomerase 789271 789945 1 675 P3.orf0664HTH-type transcriptional regulator 790355 790053 −3 303 P3.orf0665 iscSCysteine desulfurase 791652 790423 −1 1230 P3.orf0666 iscS Cysteinesulfinate desulfinase/cysteine 792881 791745 −3 1137 desulfurase andrelated enzyme P3.orf0667 iscR transcriptional regulator, BadM/Rrf2family 793399 792878 −2 522 P3.orf0668 cysE serine O-acetyltransferase794181 793441 −1 741 P3.orf0669 hydrolase of the alpha/beta superfamily794379 795017 3 639 P3.orf0670 thrC threonine synthase-like protein796344 795088 −1 1257 P3.orf0671 siaT TRAP transporter, 4TM/12TM fusionprotein 798272 796416 −3 1857 P3.orf0672 TRAP transporter solutereceptor, TAXI family 799414 798431 −2 984 P3.orf0673 kdgR IclR familytranscriptional regulator 800322 799525 −1 798 P3.orf0674 pepQ peptidaseM24 801581 800361 −3 1221 P3.orf0675 zntA heavy metal translocatingP-type ATPase 804002 801801 −3 2202 P3.orf0676 zntR transcriptionalregulator, MerR family protein 804141 804641 3 501 P3.orf0677 rsmAphospholipid N-methyltransferase protein 804744 805394 3 651 P3.orf0678anmK anhydro-N-acetylmuramic acid kinase 807521 805377 −3 2145P3.orf0679 tyrS tyrosyl-tRNA synthetase 807709 808965 1 1257 P3.orf0680YceI family protein 809682 809047 −1 636 P3.orf0681 slyA MarR familytranscriptional regulator 809891 810358 2 468 P3.orf0682 majorfacilitator transporter 810360 811934 3 1575 P3.orf0683 emrA secretionprotein HlyD 811942 812994 1 1053 P3.orf0684 conserved hypotheticalprotein 816400 813002 −2 3399 P3.orf0685 glnE glutamate-ammonia-ligaseadenylyltransferase 816637 819669 1 3033 P3.orf0686 bcp Redoxin 819666820127 3 462 P3.orf0687 conserved hypothetical protein 820143 821033 3891 P3.orf0688 yncA GCN5-related N-acetyltransferase 821069 821593 2 525P3.orf0689 conserved hypothetical protein 821947 822381 1 435 P3.orf0690conserved hypothetical protein 824286 823750 −1 537 P3.orf0691 conservedhypothetical protein 823753 822395 −2 1359 P3.orf0692 conservedhypothetical protein 824445 825173 3 729 P3.orf0693 major facilitatorsuperfamily MFS_1 826398 825151 −1 1248 P3.orf0694 ydeM putative acyldehydratase 826905 826429 −1 477 P3.orf0695 ubiquinone/menaquinonebiosynthesis 827735 826902 −3 834 methyltransferase protein P3.orf0696radical SAM domain protein 830039 827772 −3 2268 P3.orf0697 rhiR LuxRfamily transcriptional regulator 830445 831188 3 744 P3.orf0698 rhlIN-acyl-L-homoserine lactone synthetase MsaI 831221 831901 2 681P3.orf0699 ydeM MaoC domain protein dehydratase 831898 832386 1 489P3.orf0700 alpha/beta hydrolase fold protein 833473 832415 −2 1059P3.orf0701 prfB peptide chain release factor RF-2 834438 833473 −1 966P3.orf0702 mrcA penicillin binding protein 1A 837211 834671 −2 2541P3.orf0703 amiC N-acetylmuramoyl-L-alanine amidase 838844 837453 −3 1392P3.orf0704 rne Ribonuclease E and G 839847 843209 3 3363 P3.orf0705 aspCputative aminotransferase protein 844504 843335 −2 1170 P3.orf0706 GGT1gamma-glutamyltranspeptidase precursor 845878 844637 −2 1242 P3.orf0707putative Zn-dependent protease 845982 847358 3 1377 P3.orf0708 outermembrane protein 847424 848182 2 759 P3.orf0709 gph phosphoglycolatephosphatase 848873 848187 −3 687 P3.orf0710 conserved hypotheticalprotein 849082 849558 1 477 P3.orf0711 vagC SpoVT/AbrB-like protein849653 849895 2 243 P3.orf0712 PilT protein domain protein 849994 8502961 303 P3.orf0713 conserved hypothetical protein 850860 850378 −1 483P3.orf0714 nahR LysR family transcriptional regulator 850859 851845 2987 P3.orf0715 bdlA methyl-accepting chemotaxis sensory 852122 853828 21707 transducer with Pas/Pac sensor P3.orf0716 fumarylacetoacetatehydrolase family protein 854906 853923 −3 984 P3.orf0717 homogentisate12-dioxygenase 856060 854909 −2 1152 P3.orf0718 vllY4-hydroxyphenylpyruvate dioxygenase 857153 856053 −3 1101 P3.orf0719transcriptional regulator, MarR family protein 857344 857895 1 552P3.orf0720 methyl-accepting chemotaxis sensory 858317 859390 2 1074transducer P3.orf0721 cya adenylate cyclase protein 859494 861215 3 1722P3.orf0722 Argininosuccinate lyase 2 862511 861219 −3 1293 P3.orf0723conserved hypothetical protein 863485 862508 −2 978 P3.orf0724 fattyacid desaturase 864225 863482 −1 744 P3.orf0725 conserved hypotheticalprotein 865333 864473 −2 861 P3.orf0726 iolS aldo/keto reductase 866343865357 −1 987 P3.orf0727 lgrD amino acid adenylation domain protein869822 866340 −3 3483 P3.orf0728 gshB glutathione synthase 871153 870191−2 963 P3.orf0729 GST glutathione S-transferase 872056 871313 −2 744P3.orf0730 thiG bifunctional sulfur carrier protein/thiazole 873100872105 −2 996 synthase protein P3.orf0731 aroQ 3-dehydroquinatedehydratase 873253 873732 1 480 P3.orf0732 accB acetyl-CoA carboxylase,biotin carboxyl carrier 873725 874207 2 483 protein P3.orf0733 accCacetyl-CoA carboxylase 874221 875564 3 1344 P3.orf0734 D-serinedehydratase 875663 876799 2 1137 P3.orf0735 ycjX conserved hypotheticalprotein 876931 878382 1 1452 P3.orf0736 membrane protein 878379 879446 31068 P3.orf0737 leucyl/phenylalanyl-tRNA--protein transferase 879579880301 3 723 P3.orf0738 conserved hypothetical protein 880751 880308 −3444 P3.orf0739 ABC superfamily ATP binding cassette 881269 880748 −2 522transporter substrate binding protein P3.orf0740 NADH dehydrogenase881697 881350 −1 348 P3.orf0741 nrdJ Ribonucleotide reductase largesubunit 882342 886010 3 3669 P3.orf0742 putative aminopeptidase protein886135 886365 1 231 P3.orf0743 domain of unknown function DUF1814 886362887063 3 702 P3.orf0744 nuclease 888130 887069 −2 1062 P3.orf0745conserved hypothetical protein 889257 888127 −1 1131 P3.orf0746conserved hypothetical protein 890082 889258 −1 825 P3.orf0747 putativetransmembrane protein 891508 890066 −2 1443 P3.orf0748 conservedhypothetical protein 892071 891652 −1 420 P3.orf0749 IivG ABCtransporter related protein 892719 893525 3 807 P3.orf0750 IivF ABCtransporter related protein 893545 894264 1 720 P3.orf0751 IivHhydrophobic amino acid ABC transporter 894261 895124 3 864 permeaseP3.orf0752 braE inner-membrane translocator 895124 896110 2 987P3.orf0753 nepI major facilitator family transporter 897320 896151 −31170 P3.orf0754 yafC Transcriptional regulator, LysR family 898325897456 −3 870 P3.orf0755 mauR LysR family transcriptional regulator899245 898304 −2 942 P3.orf0756 sodium/sulphate symporter 899382 9007883 1407 P3.orf0757 Glyoxalase/bleomycin resistance 901172 900798 −3 375protein/dioxygenase P3.orf0758 conserved hypothetical protein 902518901277 −2 1242 P3.orf0759 sigma-54 interacting transcription regulator902714 904321 2 1608 protein P3.orf0760 nsrR HTH-type transcriptionalregulator nsrR 904774 904325 −2 450 P3.orf0761 braC extracellularligand-binding receptor 906172 904952 −2 1221 P3.orf0762 hypotheticalprotein 906686 906889 2 204 P3.orf0763 xdhA aldehyde oxidase andxanthine 907429 910212 1 2784 dehydrogenase molybdopterin bindingP3.orf0764 hcrC putative deshydrogenase/oxidoreductase; 910209 910781 3573 P3.orf0765 Gluconate 2-dehydrogenase (acceptor) 910778 912058 2 1281P3.orf0766 AMP-dependent synthetase and ligase 913887 912082 −1 1806P3.orf0767 IivF ABC transporter related protein 914711 913884 −3 828P3.orf0768 IivK Extracellular ligand-binding receptor 916006 914780 −21227 P3.orf0769 IivM inner-membrane translocator 917130 916087 −1 1044P3.orf0770 IivH inner-membrane translocator 918063 917185 −1 879P3.orf0771 braF ABC transporter related protein 918916 918149 −2 768P3.orf0772 yqjP Beta-lactamase-like 920316 919276 −1 1041 P3.orf0773acyl-CoA dehydrogenase 922322 920526 −3 1797 P3.orf0774 hypotheticalprotein 923029 922643 −2 387 P3.orf0775 asnO asparagine synthase 925222923261 −2 1962 P3.orf0776 conserved hypothetical protein 925873 925373−2 501 P3.orf0777 sir1 sulfite reductase (ferredoxin) 927872 925857 −32016 P3.orf0778 cya adenylate cyclase 1 929331 928132 −1 1200 P3.orf0779conserved hypothetical protein 929415 929771 3 357 P3.orf0780 2OG-Fe(II)oxygenase 930407 929787 −3 621 P3.orf0781 conserved hypothetical protein930792 932603 3 1812 P3.orf0782 mmgC acyl-CoA dehydrogenase 934470932680 −1 1791 P3.orf0783 fadN 3-hydroxyacyl-CoA dehydrogenase 936829934496 −2 2334 P3.orf0784 fadA 3-ketoacyl-CoA thiolase 938014 936875 −21140 P3.orf0785 transcriptional regulator, MerR family protein 938540938148 −3 393 P3.orf0786 lcfA long-chain-fatty-acid--CoA ligase 940431938701 −1 1731 P3.orf0787 Helix-turn-helix motif protein 941092 940724−2 369 P3.orf0788 plasmid maintenance system killer 941296 941174 −2 123P3.orf0789 hypothetical protein 942032 941577 −3 456 P3.orf0790conserved hypothetical protein 942675 942043 −1 633 P3.orf0791 conservedhypothetical protein 943429 942773 −2 657 P3.orf0792 ArgK protein 944530943568 −2 963 P3.orf0793 ppdK pyruvate phosphate dikinase 947228 944574−3 2655 P3.orf0794 glyS glycyl-tRNA synthetase beta chain 949324 947249−2 2076 P3.orf0795 hypothetical protein 950163 950405 3 243 P3.orf0796glyQ Glycyl-tRNA synthetase alpha chain 950135 949329 −3 807 P3.orf0797hypothetical protein 950700 950449 −1 252 P3.orf0798 peptidase S49951588 950713 −1 876 P3.orf0799 methyltransferase small 952466 951648 −3819 P3.orf0800 conserved hypothetical protein 952771 952463 −2 309P3.orf0801 ispB Polyprenyl synthetase 953124 954125 3 1002 P3.orf0802pleC integral membrane sensor hybrid histidine 954513 955805 3 1293kinase P3.orf0803 ADS1 fatty-acid desaturase 955953 956948 3 996P3.orf0804 ppsA Beta-ketoacyl synthase 957140 957436 2 297 P3.orf0805cyclopropane-fatty-acyl-phospholipid synthase 957464 958363 2 900P3.orf0806 amino acid adenylation 958379 960163 2 1785 P3.orf0807acyl-CoA dehydrogenase domain protein 960167 961330 2 1164 P3.orf0808crt F148266_1 unknown 961327 962502 1 1176 P3.orf0809 cmoAmethyltransferase type 12 963218 962508 −3 711 P3.orf0810 conservedhypothetical protein 963792 963313 −1 480 P3.orf0811 conservedhypothetical protein 964293 963898 −1 396 P3.orf0812 HDDC2 protein964561 965175 1 615 P3.orf0813 ycfQ transcriptional regulator, TetRfamily 965820 965188 −1 633 P3.orf0814 estB beta-lactamase 965903 9671502 1248 P3.orf0815 TetR-family transcriptional regulator 967743 967138 −1606 P3.orf0816 gst3 glutathione S-transferase domain-containing 967843968511 1 669 protein P3.orf0817 conserved hypothetical protein 969588968770 −1 819 P3.orf0818 yfhB phenazine biosynthesis protein 971074970151 −2 924 P3.orf0819 GntR family transcriptional regulator with971121 972512 3 1392 aminotransferase domain P3.orf0820 putativeflagellin 972583 974163 1 1581 P3.orf0821 conserved hypothetical protein974616 974173 −1 444 P3.orf0822 FMO5 Dimethylaniline monooxygenase[N-oxide- 976226 974622 −3 1605 forming] 5 P3.orf0823 biotin/lipoylattachment domain-containing 976537 976319 −2 219 protein P3.orf0824accC carbamoyl-phosphate synthase L chain ATP- 977933 976590 −3 1344binding P3.orf0825 conserved hypothetical protein 978049 978747 1 699P3.orf0826 transcriptional regulator, XRE family 978834 979655 3 822P3.orf0827 ybhB putative phosphatidylethanolamine-binding 980209 979643−2 567 protein P3.orf0828 ybcM AraC protein 981184 980306 −2 879P3.orf0829 conserved hypothetical protein 981345 982268 3 924 P3.orf0830rhtB amino acid efflux protein 982913 982272 −3 642 P3.orf0831 mutBmethylmalonyl-CoA mutase 985204 983015 −2 2190 P3.orf0832 mutAmethylmalonyl-CoA mutase 987113 985209 −3 1905 P3.orf0833 Enoyl-CoAhydratase/isomerase 988058 987369 −3 690 P3.orf0834 hypothetical protein988638 988207 −1 432 P3.orf0835 hypothetical protein 989373 988879 −1495 P3.orf0836 hmuR TonB-dependent receptor plug 989660 991966 2 2307P3.orf0837 yddA putative ABC transport system, ATP-binding 991971 9937553 1785 protein P3.orf0838 yrpG putative oxidoreductase 994690 993740 −2951 P3.orf0839 3-ketoacyl-(acyl-carrier-protein) reductase 995724 994966−1 759 P3.orf0840 NHL repeat-containing protein 996113 997576 2 1464P3.orf0841 GCN5-related N-acetyltransferase 997593 998120 3 528P3.orf0842 npdA NAD-dependent deacetylase 998882 998127 −3 756P3.orf0843 nitro reductase 999578 998934 −3 645 P3.orf0844 hypotheticalprotein 999914 999597 −3 318 P3.orf0845 pleC multi-sensor signaltransduction histidine 1002236 1000074 −3 2163 kinase P3.orf0846ATP-dependent protease HslVU (ClpYQ), 1003513 1002365 −2 1149 ATPasesubunit P3.orf0847 cytochrome c family protein 1003815 1004216 3 402P3.orf0848 conserved hypothetical protein 1004257 1004955 1 699P3.orf0849 acoR transcriptional regulator 1005170 1007215 2 2046P3.orf0850 acoX ATP-NAD/AcoX kinase 1007579 1008661 2 1083 P3.orf0851acoA acetoin dehydrogenase complex, E1 1008740 1009744 2 1005 component,alpha subunit P3.orf0852 acoB pyruvate dehydrogenase E1 component, beta1009779 1010786 3 1008 subunit P3.orf0853 acoC branched-chain alpha-ketoacid 1010805 1011917 3 1113 dehydrogenase subunit E2 P3.orf0854 budCshort-chain dehydrogenase/reductase SDR 1011922 1012716 1 795 P3.orf0855hypothetical protein 1013102 1013245 2 144 P3.orf0856 Lysine-specificdemethylase 1013381 1014550 2 1170 P3.orf0857 aspartyl/asparaginylbeta-hydroxylase 1015527 1014553 −1 975 P3.orf0858 conservedhypothetical protein 1015809 1015531 −1 279 P3.orf0859 prolyl4-hydroxylase, alpha subunit 1015971 1016600 3 630 P3.orf0860 conservedhypothetical protein 1016930 1016616 −3 315 P3.orf0861 asnO Asparaginesynthase (glutamine-hydrolyzing) 1017430 1019361 1 1932 P3.orf0862sulfotransferase 1020238 1019384 −2 855 P3.orf0863 HPr kinase 10210711020238 −1 834 P3.orf0864 viuB siderophore-interacting protein 10223431021240 −1 1104 P3.orf0865 solute-binding periplasmic protein of 10235211022385 −3 1137 iron/siderophore ABC transporter P3.orf0866 fhuA OMRfamily ferrichrome outer membrane 1025656 1023536 −2 2121 transporterP3.orf0867 TetR family transcriptional regulator 1025958 1026647 3 690P3.orf0868 alpha/beta hydrolase fold 1027782 1026658 −1 1125 P3.orf0869paaG enoyl-CoA hydratase 1028657 1027845 −3 813 P3.orf0870 Acetyl-CoAC-acetyltransferase 1029949 1028765 −2 1185 P3.orf0871 yncBoxidoreductase, zinc-binding dehydrogenase 1030187 1031251 2 1065 familyP3.orf0872 pleC two-component sensor histidine kinase 1031517 1032734 31218 P3.orf0873 putative lipoprotein 1033471 1032830 −2 642 P3.orf0874hypothetical protein 1033737 1033501 −1 237 P3.orf0875 putative2-hydroxychromene-2-carboxylate 1034622 1034008 −1 615 isomeraseP3.orf0876 5-carboxymethyl-2-hydroxymuconate delta- 1034781 1035623 3843 isomerase P3.orf0877 yjcC conserved hypothetical protein 10358291037511 1 1683 P3.orf0878 conserved hypothetical protein 1038050 1037535−3 516 P3.orf0879 transcriptional regulator, XRE family 1038394 10389991 606 P3.orf0880 sulfonate/nitrate/taurine transport system ATP- 10396641040437 2 774 binding protein P3.orf0881 sulfonate/nitrate/taurinetransport system 1040430 1041365 3 936 permease protein P3.orf0882sulfonate/nitrate/taurine transport system 1041362 1042516 2 1155permease protein P3.orf0883 sulfonate/nitrate/taurine transport system1042687 1043700 1 1014 substrate-binding protein P3.orf0884 diguanylatecyclase/phosphodiesterase with 1043849 1045435 2 1587 PAS/PAC sensor(s)P3.orf0885 ytsP conserved hypothetical protein 1045456 1045956 1 501P3.orf0886 yhjX major facilitator superfamily MFS_1 1047664 1046012 −21653 P3.orf0887 cynR LysR-family transcriptional regulator 10488391047913 −1 927 P3.orf0888 yhcA EmrB/QacA family drug resistancetransporter 1050376 1048943 −2 1434 P3.orf0889 slyA regulatory protein,MarR 1050906 1050373 −1 534 P3.orf0890 kipR IclR family regulatoryprotein 1051954 1051049 −2 906 P3.orf0891 acoA Thiaminepyrophosphate-dependent 1052403 1053386 3 984 dehydrogenase, E1component alpha subunit P3.orf0892 pdhB putative pyruvate dehydrogenaseE1 beta 1053383 1054354 2 972 subunit P3.orf0893 outer membrane effluxprotein 1056161 1054611 −3 1551 P3.orf0894 stan VCBS 1059657 1056286 −13372 P3.orf0895 Fat4 Putative Ig domain family 1076476 1059644 −2 16833P3.orf0896 conserved hypothetical protein 1082145 1076527 −1 5619P3.orf0897 conserved hypothetical protein 1082623 1082946 1 324P3.orf0898 Tail Collar domain protein 1082954 1083649 2 696 P3.orf0899tail collar domain-containing protein 1083750 1084343 3 594 P3.orf0900phage tail collar domain-containing protein 1084375 1084974 1 600P3.orf0901 phage tail collar domain-containing protein 1085296 1085622 1327 P3.orf0902 peptidase M50 1088761 1086569 −2 2193 P3.orf0903 mdtAMultidrug resistance protein mdtA 1090330 1088765 −2 1566 P3.orf0904conserved hypothetical protein 1091271 1090327 −1 945 P3.orf0905conserved hypothetical protein 1092302 1091397 −3 906 P3.orf0906 samS-adenosylmethionine uptake transporter 1092538 1093491 1 954 P3.orf0907yodQ acetylornithine deacetylase or succinyl- 1093542 1094795 3 1254diaminopimelate desuccinylase P3.orf0908 conserved hypothetical protein1095826 1095146 −2 681 P3.orf0909 ydjG aldo/keto reductase 10967971095826 −1 972 P3.orf0910 membrane protein 1097557 1096910 −2 648P3.orf0911 hypothetical protein 1097741 1097571 −3 171 P3.orf0912hypothetical protein 1098183 1097731 −1 453 P3.orf0913 fabG short-chaindehydrogenase/reductase SDR 1099050 1098265 −1 786 P3.orf0914 linXshort-chain dehydrogenase/reductase SDR 1099871 1099077 −3 795P3.orf0915 yiaN DctM9 1101186 1099900 −1 1287 P3.orf0916 DctQ9 11017221101183 −3 540 P3.orf0917 siaP TRAP dicarboxylate transporter, DctPsubunit 1102788 1101802 −1 987 P3.orf0918 hypothetical protein 11032011103016 −3 186 P3.orf0919 fixI cation transport ATPase 1105679 1103229−3 2451 P3.orf0920 nitrogen fixation protein fixH 1106284 1105700 −2 585P3.orf0921 fixG nitrogen fixation protein fixG 1107868 1106360 −2 1509P3.orf0922 petJ cytochrome-c oxidase fixP 1108809 1107940 −1 870P3.orf0923 cytochrome-c oxidase FixO2 1109728 1109003 −2 726 P3.orf0924fixN cbb3-type cytochrome c oxidase subunit I 1111223 1109733 −3 1491P3.orf0925 hypothetical protein 1111513 1111256 −2 258 P3.orf0926conserved hypothetical protein 1111787 1112620 2 834 P3.orf0927hypothetical protein 1112860 1112642 −2 219 P3.orf0928 IivF ABCtransporter ATP-binding protein 1113652 1112957 −2 696 P3.orf0929 IivGABC transporter ATP-binding protein 1114401 1113649 −1 753 P3.orf0930ABC transporter permease protein 1115462 1114398 −3 1065 P3.orf0931 IivHinner-membrane translocator 1116426 1115491 −1 936 P3.orf0932Extracellular ligand-binding receptor 1117676 1116489 −3 1188 P3.orf0933transcriptional regulator, AsnC family protein 1118010 1117768 −1 243P3.orf0934 conserved hypothetical protein 1119103 1119681 1 579P3.orf0935 GCN5-related N-acetyltransferase 1119806 1121011 2 1206P3.orf0936 lysX RimK domain protein ATP-grasp 1121016 1122521 3 1506P3.orf0937 yncD putative TonB-dependent receptor protein 1122565 11249881 2424 P3.orf0938 transcriptional regulator 1125283 1124993 −2 291P3.orf0939 addiction module killer protein 1125581 1125276 −3 306P3.orf0940 thioesterase superfamily protein 1126135 1125626 −2 510P3.orf0941 menE O-succinylbenzoate--CoA ligase 1126640 1126176 −3 465P3.orf0942 fadD AMP-dependent synthetase and ligase 1126818 1126660 −1159 P4.orf0001 hcpC Putative beta-lactamase hcpC 1 1233 1 1233P4.orf0002 rifampin ADP-ribosylating transferase 1724 1275 −3 450P4.orf0003 conserved hypothetical protein 2205 1702 −1 504 P4.orf0004ThiJ/PfpI 3391 2255 −2 1137 P4.orf0005 glxA transcriptional regulator,AraC family 3495 4505 3 1011 P4.orf0006 pleC Signal transductionhistidine kinase 6703 4697 −2 2007 P4.orf0007 putative endonucleaseinvolved in 7431 6823 −1 609 recombination P4.orf0008 gatCaspartyl/glutamyl-tRNA(Asn/Gln) 7647 7934 3 288 amidotransferase subunitC P4.orf0009 gatA aspartyl/glutamyl-tRNA(Asn/Gln) 7931 9433 2 1503amidotransferase subunit A P4.orf0010 gatBaspartyl-tRNA(Asn)/glutamyl-tRNA (Gln) 9430 10881 1 1452amidotransferase subunit B P4.orf0011 hypothetical protein 11112 11450 3339 P4.orf0012 Putative lipoprotein 11505 12014 3 510 P4.orf0013 yafPN-acetyltransferase yafP 12042 12503 3 462 P4.orf0014 CopG familyDNA-binding protein 12528 12803 3 276 P4.orf0015 hypothetical protein13120 12971 −2 150 P4.orf0016 toxin complex protein 13164 20768 3 7605P4.orf0017 metI binding-protein-dependent transport systems 21511 20828−2 684 inner membrane component P4.orf0018 metN D-methionine transportsystem ATP-binding 22577 21495 −3 1083 protein P4.orf0019 ygcF7-carboxy-7-deazaguanine synthase homolog 22800 23543 3 744 P4.orf0020queD putative 6-pyruvoyl tetrahydropterin synthase 23543 23929 2 387P4.orf0021 queC exsB protein 23926 24669 1 744 P4.orf0022 23S rRNAmethyltransferase 24686 25204 2 519 P4.orf0023 menE AMP-dependentsynthetase and ligase 26973 25480 −1 1494 P4.orf0024 fadRtranscriptional regulator AcrR family 27670 27053 −2 618 P4.orf0025conserved hypothetical protein 28000 28827 1 828 P4.orf0026 putativeRossmann fold nucleotide-binding 29784 30428 3 645 protein P4.orf0027glpR transcriptional regulator 29699 28854 −3 846 P4.orf0028 phnV ABCtransporter permease protein 30599 31381 2 783 P4.orf0029 potA ABCtransporter ATP-binding protein 31392 32417 3 1026 P4.orf0030spermidine/putrescine transport system 32495 33592 2 1098substrate-binding protein P4.orf0031 potB Spermidine/putrescinetransport system 33705 34526 3 822 permease protein P4.orf0032 siaPTRAP-type bacterial extracellular solute- 35753 34626 −3 1128 bindingprotein P4.orf0033 siaT TrapT family protein 37113 35791 −1 1323P4.orf0034 Tripartite ATP-independent periplasmic 37598 37110 −3 489P4.orf0035 glpR putative transcriptional regulator 38445 37690 −1 756P4.orf0036 oxidoreductase, FAD-binding protein 38634 40082 3 1449P4.orf0037 ooxA Opine oxidase subunit A 40100 41329 2 1230 P4.orf0038glpK glycerol kinase 41326 42756 1 1431 P4.orf0039 conservedhypothetical protein 43607 42732 −3 876 P4.orf0040 hypothetical protein43635 43880 3 246 P4.orf0041 citE Citrate lyase 44886 43918 −1 969P4.orf0042 puuR XRE family-like protein 45473 44883 −3 591 P4.orf0043artJ ABC-type amino acid transport 45870 46616 3 747 P4.orf0044 glnMamino acid ABC transporter permease protein 46777 47400 1 624 P4.orf0045tcyC amino acid ABC transporter ATP-binding 47397 48128 3 732 proteinP4.orf0046 puuB FAD dependent oxidoreductase 48489 49658 3 1170P4.orf0047 putative bleomycin resistance protein 50018 49659 −3 360P4.orf0048 fhuC ABC transporter related protein 50845 50063 −2 783P4.orf0049 yojI pyoverdine biosynthesis protein PvdE 52485 50845 −1 1641P4.orf0050 viuB FAD-binding 9 siderophore-interacting 53240 52482 −3 759domain-containing protein P4.orf0051 fhuB transport system permeaseprotein 55216 53237 −2 1980 P4.orf0052 fhuD iron complex transportsystem substrate- 56103 55213 −1 891 binding protein P4.orf0053 foxAputative TonB-dependent receptor 58628 56106 −3 2523 P4.orf0054 fecRFecR protein 59723 58737 −3 987 P4.orf0055 sigW DNA-directed RNApolymerase specialized 60330 59815 −1 516 sigma subunit P4.orf0056conserved hypothetical protein 60860 60522 −3 339 P4.orf0057 conservedhypothetical protein 61234 62583 1 1350 P4.orf0058 mrp protein 6386162650 −1 1212 P4.orf0059 hflK HflK protein 64296 65366 3 1071 P4.orf0060hflC membrane protease subunit HflC 65385 66287 3 903 P4.orf0061conserved hypothetical protein 66495 66776 3 282 P4.orf0062 conservedhypothetical protein 67990 68175 1 186 P4.orf0063 degP Peptidase S1C, Do68267 69739 2 1473 P4.orf0064 tas aldo/keto reductase 67819 66809 −21011 P4.orf0065 phosphoserine phosphatase SerB 70742 69825 −3 918P4.orf0066 miaA tRNA delta(2)-isopentenylpyrophosphate 70814 71782 2 969transferase P4.orf0067 ilvI acetolactate synthase, large subunit 7202173772 3 1752 P4.orf0068 ilvH acetolactate synthase 3 regulatory subunit73882 74418 1 537 P4.orf0069 ilvC ketol-acid reductoisomerase 7448075607 2 1128 P4.orf0070 yfkH ribonuclease BN 75627 76763 3 1137P4.orf0071 conserved hypothetical protein 77386 76790 −2 597 P4.orf0072conserved hypothetical protein 77913 77488 −1 426 TM.orf0001 dnaAchromosomal replication initiation protein 1 1578 1 1578 TM.orf0002 dnaNDNA polymerase III subunit beta 1786 2907 1 1122 TM.orf0003 recFrecombination protein F 2904 4169 2 1266 TM.orf0004 gyrB DNA gyrasesubunit B 4183 6687 1 2505 TM.orf0005 putative transcription regulatorprotein 6772 6981 1 210 TM.orf0006 conserved hypothetical protein 69787244 2 267 TM.orf0007 ygiD Catalytic LigB subunit of aromaticring-opening 8093 7278 −2 816 dioxygenase TM.orf0008 pbpG penicillinbinding protein 8240 10417 3 2178 TM.orf0009 bcr drug resistancetransporter, Bcr/CflA subfamily 11649 10429 −1 1221 TM.orf0010 citACitrate (Si)-synthase 12169 13266 1 1098 TM.orf0011 conservedhypothetical protein 14238 13351 −1 888 TM.orf00122-amino-3-carboxymuconate-6-semialdehyde 15546 14329 −1 1218decarboxylase TM.orf0013 leuA pyruvate carboxyltransferase 16189 17394 11206 TM.orf0014 Pc06g00060 17400 18092 2 693 TM.orf0015 CADD protein18203 18883 3 681 TM.orf0016 potC binding-protein-dependent transportsystems 19730 18945 −2 786 inner membrane component TM.orf0017 potHbinding-protein-dependent transport systems 20912 19737 −2 1176 innermembrane component TM.orf0018 potA putative spermidine/putrescine ABC21979 20909 −3 1071 transporter, ATP-binding protein TM.orf0019spermidine/putrescine-binding periplasmic 23147 22092 −2 1056 proteinTM.orf0020 ABC-2 type transporter 24256 23381 −3 876 TM.orf0021 nodI ABCtransporter related protein 25005 24253 −1 753 TM.orf0022 conservedhypothetical protein 25155 25655 2 501 TM.orf0023 regB two-componentsensor histidine kinase 25652 27001 3 1350 TM.orf0024 regAphotosynthetic apparatus regulatory protein 27055 27645 1 591 RegATM.orf0025 yneP thioesterase family protein 27784 28308 1 525 TM.orf0026mprA two component transcriptional regulator, 29239 28325 −3 915 wingedhelix family TM.orf0027 argD acetylornithine and succinylornithine 2953330732 1 1200 aminotransferase TM.orf0028 argF ornithinecarbamoyltransferase 30729 31256 2 528 TM.orf0029 argF ornithinecarbamoyltransferase 31267 31704 1 438 TM.orf0030 hslO molecularchaperone Hsp33 31701 32687 2 987 TM.orf0031 Acyl-CoA synthetases(AMP-forming)/AMP- 32743 33399 1 657 acid ligases II TM.orf0032 gylRIclR family regulatory protein 33543 34448 2 906 TM.orf0033 menCN-acylamino acid racemase 35583 34474 −1 1110 TM.orf0034 beta-lactamase37011 35704 −1 1308 TM.orf0035 nudF adp-ribose pyrophosphatase 3762037156 −1 465 TM.orf0036 TetR family transcriptional regulator 3827137663 −1 609 TM.orf0037 crp transcriptional regulator 38805 39485 2 681TM.orf0038 fixR halohydrin epoxidase A 39570 40316 2 747 TM.orf0039hypothetical protein 40712 40512 −2 201 TM.orf0040 secretion activatorprotein 41274 40729 −1 546 TM.orf0041 hypothetical protein 41399 41271−2 129 TM.orf0042 putative tail fiber protein 43942 41588 −3 2355TM.orf0043 conserved hypothetical protein 46922 43947 −2 2976 TM.orf0044conserved hypothetical protein 49201 46919 −3 2283 TM.orf0045 conservedhypothetical protein 52092 49198 −1 2895 TM.orf0046 hypothetical protein52646 52092 −2 555 TM.orf0047 conserved hypothetical protein 54580 52646−3 1935 TM.orf0048 conserved hypothetical protein 55198 54584 −3 615TM.orf0049 hypothetical protein 55520 55203 −2 318 TM.orf0050 conservedhypothetical protein 56404 55535 −3 870 TM.orf0051 hypothetical protein57586 56708 −3 879 TM.orf0052 hypothetical protein 57893 57606 −2 288TM.orf0053 conserved hypothetical protein 59500 57890 −3 1611 TM.orf0054conserved hypothetical protein 60285 59713 −1 573 TM.orf0055 conservedhypothetical protein 61524 60295 −1 1230 TM.orf0056 conservedhypothetical protein 62297 61770 −2 528 TM.orf0057 DNA primase 6446562915 −3 1551 TM.orf0058 conserved hypothetical protein 65855 64749 −21107 TM.orf0059 hypothetical protein 65984 66289 3 306 TM.orf0060hypothetical protein 66645 66322 −1 324 TM.orf0061 conservedhypothetical protein 66920 66645 −2 276 TM.orf0062 hypothetical protein67428 67204 −1 225 TM.orf0063 conserved hypothetical protein 67775 67425−2 351 TM.orf0064 hypothetical protein 68454 68194 −1 261 TM.orf0065conserved hypothetical protein 68687 68451 −2 237 TM.orf0066hypothetical protein 69642 69863 2 222 TM.orf0067 hypothetical protein69856 70269 1 414 TM.orf0068 conserved hypothetical protein 70675 713911 717 TM.orf0069 hypothetical protein 71388 71807 2 420 TM.orf0070hypothetical protein 71804 72085 3 282 TM.orf0071 hypothetical protein72105 72434 2 330 TM.orf0072 hypothetical protein 72421 72678 1 258TM.orf0073 hypothetical protein 72678 72995 2 318 TM.orf0074 phageintegrase family protein 72995 74203 3 1209 TM.orf0075 hypotheticalprotein 74694 74437 −1 258 TM.orf0076 hypothetical protein 75004 75219 1216 TM.orf0077 hypothetical protein 75230 75640 3 411 TM.orf0078conserved hypothetical protein 75646 75903 1 258 TM.orf0079 L-lactatepermease 77675 76167 −2 1509 TM.orf0080 hypothetical protein 77831 793003 1470 TM.orf0081 fh fumarate hydratase, class II 80821 79415 −3 1407TM.orf0082 HemY protein 82392 80995 −1 1398 TM.orf0083 conservedhypothetical protein 83888 82389 −2 1500 TM.orf0084 hemDuroporphyrinogen III synthase HEM4 84784 84008 −3 777 TM.orf0085 HEMCPorphobilinogen deaminase, chloroplastic 85834 84845 −3 990 TM.orf0086gcp O-sialoglycoprotein endopeptidase 85940 87097 3 1158 TM.orf0097conserved hypothetical protein 88155 87109 −1 1047 TM.orf0088 conservedhypothetical protein 89324 88152 −2 1173 TM.orf0089 conservedhypothetical protein 90512 89349 −2 1164 TM.orf0090 transmembraneprotein 91469 90630 −2 840 TM.orf0091 NmrA-like protein 92583 91600 −1984 TM.orf0092 ycil YCIl-related protein 92841 93125 2 285 TM.orf0093Thymocyte nuclear protein 93147 93566 2 420 TM.orf0094 petC Rieske 9356893927 1 360 TM.orf0095 acsA acetyl-coenzyme A synthetase 94040 95998 31959 TM.orf0096 GMC oxidoreductase 97714 96806 −3 909 TM.orf0097hypothetical protein 98517 98398 −1 120 TM.orf0098 conservedhypothetical protein 99271 98888 −3 384 TM.orf0099 conservedhypothetical protein 99605 99426 −2 180 TM.orf0100 htpX heat shockprotein HtpX 99762 100673 2 912 TM.orf0101 rsmB Sun protein 100681102306 1 1626 TM.orf0102 rpe ribulose-phosphate 3-epimerase 102358103083 1 726 TM.orf0103 conserved hypothetical protein 103162 105234 12073 TM.orf0104 rrf2 FeS assembly SUF system regulator 105385 105822 1438 TM.orf0105 FeS assembly protein SufB 105949 107394 1 1446 TM.orf0106sufC FeS assembly ATPase SufC 107488 108246 1 759 TM.orf0107 FeSassembly protein SufD 108251 109600 3 1350 TM.orf0108 sufS cysteinedesulfurase, SufS subfamily 109597 110844 1 1248 TM.orf0109 nifU NifUfamily SUF system FeS assembly protein 110856 111359 2 504 TM.orf0110yitW conserved hypothetical protein 111435 111776 2 342 TM.orf0111conserved hypothetical protein 111898 112584 1 687 TM.orf0112 mhqRtranscriptional regulator, MarR family 112665 113168 2 504 TM.orf0113conserved hypothetical protein 113153 113515 3 363 TM.orf0114 ydfHtranscriptional regulatory protein 114188 113553 −2 636 TM.orf0115choline/carnitine/betaine transporter 116182 114206 −3 1977 TM.orf0116hypothetical protein 116447 116590 3 144 TM.orf0117 ybbK conservedhypothetical protein 117109 116603 −3 507 TM.orf0118 meaAmethylmalonyl-CoA mutase 119139 117109 −1 2031 TM.orf0119 crotonyl-CoAreductase 119561 120847 3 1287 TM.orf0120 yngJ Acyl-CoA dehydrogenase121098 122777 2 1680 TM.orf0121 hypothetical protein 122896 123093 1 198TM.orf0122 ATP: cob(I)alamin adenosyltransferase 123188 123763 3 576TM.orf0123 etfB Electron transfer flavoprotein, beta subunit 124053124802 2 750 TM.orf0124 etfA electron transfer flavoprotein alphasubunit 124805 125737 3 933 TM.orf0125 hbdA 3-hydroxyacyl-CoAdehydrogenase 125872 126744 1 873 TM.orf0126 Thiol: disulfideinterchange protein TlpA 127403 126807 −2 597 TM.orf0127 argHargininosuccinate lyase 127533 128948 2 1416 TM.orf0128 lysAdiaminopimelate decarboxylase 129193 130452 1 1260 TM.orf0129hypoxanthine phosphoribosyltransferase 131087 130545 −2 543 TM.orf0130conserved hypothetical protein 131816 131157 −2 660 TM.orf0131 ftsEputative ATPase involved in cell division 132046 132810 1 765 TM.orf0132cell division protein 132807 133694 2 888 TM.orf0133 conservedhypothetical protein 133763 134383 3 621 TM.orf0134phospholipid/glycerol acyltransferase 134380 135102 1 723 TM.orf0135yigI thioesterase superfamily protein 135712 135278 −3 435 TM.orf0136thioesterase superfamily protein 136197 135709 −1 489 TM.orf0137 chaCCation transport protein chaC 136860 136246 −1 615 TM.orf0138 conservedhypothetical protein 137089 138096 1 1008 TM.orf0139 tyrCcyclohexadienyl dehydrogenase 139029 138133 −1 897 TM.orf0140 hisChistidinol-phosphate aminotransferase 140208 139108 −1 1101 TM.orf0141pheA chorismate mutase 141267 140347 −1 921 TM.orf0142 metX homoserineO-acetyltransferase 141696 142784 2 1089 TM.orf0143 putative methioninebiosynthesis protein 142781 143473 3 693 (MetW) TM.orf0144 pleCnon-motile and phage-resistance protein 144628 143483 −3 1146 TM.orf0145yiaO TRAP dicarboxylate transporter, DctP subunit 145134 146123 2 990TM.orf0146 tripartite ATP-independent periplasmic 146223 146735 2 513transporter DctQ TM.orf0147 TRAP dicarboxylate transporter, DctM subunit146752 148017 1 1266 TM.orf0148 SAM-dependent methyltransferases 148765147989 −3 777 TM.orf0149 gloB metallo-beta-lactamase family protein148965 149744 2 780 TM.orf0150 yibF Glutathione S-transferase 149898150503 2 606 TM.orf0151 hypothetical protein 150954 150544 −1 411TM.orf0152 conserved hypothetical protein 151812 151060 −1 753TM.orf0153 phbB acetoacetyl-CoA reductase 152634 151909 −1 726TM.orf0154 phaA acetyl-CoA acetyltransferase 153993 152821 −1 1173TM.orf0155 phbC polyhydroxyalkanoate synthase 155401 154136 −3 1266TM.orf0156 polyhydroxyalkanoate synthesis repressor 156093 156713 2 621PhaR TM.orf0157 conserved hypothetical protein 157735 156914 −3 822TM.orf0158 arcB PAS/PAC sensor hybrid histidine kinase 161874 157891 −13984 TM.orf0159 hypothetical protein 162127 162324 1 198 TM.orf0160 ABCsuperfamily ATP binding cassette 162639 163628 2 990 transporter,binding protein TM.orf0161 ABC-type transport system, permease 164038164925 1 888 component TM.orf0162 Cobalt import ATP-binding protein cbiO2 164944 165738 1 795 TM.orf0163 NUDIX hydrolase 165845 166438 3 594TM.orf0164 NHL repeat-containing protein 166621 168297 1 1677 TM.orf0165ispZ intracellular septation protein 168936 168310 −1 627 TM.orf0166ftsY Signal recognition particle GTPase 169885 168941 −3 945 TM.orf0167MiaB-like tRNA modifying enzyme 171300 169882 −1 1419 TM.orf0168 dapFdiaminopimelate epimerase 172243 171332 −3 912 TM.orf0169 divL sensorprotein divL 174357 172282 −1 2076 TM.orf0170 ahcYS-adenosyl-L-homocysteine hydrolase 175792 174497 −3 1296 TM.orf0171ptsH phosphocarrier protein HPr 176294 175995 −2 300 TM.orf0172 manX PTSsystem, IIA component 176722 176315 −3 408 TM.orf0173 3448_RHORTRecName: Full = UPF0042 177782 176838 −2 945 nucleotide-binding proteinRru_A3448 TM.orf0174 hprK HPr kinase 178240 177779 −3 462 TM.orf0175ptsN phosphotransferase system mannitol/fructose- 178760 178296 −2 465specific IIA domain-containing protein TM.orf0176 sigma 54 modulationprotein 179526 178927 −1 600 TM.orf0177 RNA polymerase factor sigma-54181269 179695 −1 1575 TM.orf0178 ABC transporter, ATP-binding protein182080 181280 −3 801 TM.orf0179 conserved hypothetical protein 182789182148 −2 642 TM.orf0180 lipopolysaccharide export system protein LptC183433 182804 −3 630 TM.orf0181 Predicted sugar phosphate isomerase184530 183538 −1 993 involved in capsule formation TM.orf0182 rnd 3′-5′exonuclease 185288 184677 −2 612 TM.orf0183 NADH-ubiquinoneoxidoreductase 39 kDa 185536 186507 1 972 subunit precursor TM.orf0184gltD putative oxidoreductase 187193 188638 3 1446 TM.orf0185 gltBglutamate synthase(NADPH) large subunit 188662 193212 1 4551 TM.orf0186gdhA Glu/Leu/Phe/Val dehydrogenase 193479 194759 2 1281 TM.orf0187 RarDprotein 194918 195850 3 933 TM.orf0188 glutathione S-transferase 195862196530 1 669 TM.orf0189 yjeS putative iron-sulfur cluster bindingprotein 196511 197725 3 1215 TM.orf0190 yeeZ NAD-dependentepimerase/dehydratase 197722 198687 1 966 TM.orf0191 ABC transporter,ATP-binding protein 200274 198694 −1 1581 TM.orf0192 hydrolase 201221200403 −2 819 TM.orf0193 Glycosyltransferase 201550 202644 1 1095TM.orf0194 rfaF ADP-heptose of LPS heptosyltransferase 202641 203612 2972 TM.orf0195 infC translation initiation factor IF-3 203868 204446 2579 TM.orf0196 rpmI 50S ribosomal protein L35 204715 204912 1 198TM.orf0197 rplT ribosomal protein L20 204999 205367 2 369 TM.orf0198pheS phenylalanyl-tRNA synthetase alpha chain 205622 206701 3 1080TM.orf0199 pheT phenylalanyl-tRNA synthetase beta chain 206718 209126 22409 TM.orf0200 cyaA putative adenylate cyclase 209411 210817 3 1407TM.orf0201 lepA GTP-binding protein 210967 212766 1 1800 TM.orf0202hypothetical protein 213156 212833 −1 324 TM.orf0203 major facilitatorsuperfamily MFS_1 214904 213621 −2 1284 TM.orf0204 regulatory proteinArsR 215206 214874 −3 333 TM.orf0205 icfA Putative carbonic anhydraseprecursor 216181 215435 −3 747 TM.orf0206 yxbA conserved hypotheticalprotein 216380 216661 3 282 TM.orf0207 GCN5-related N-acetyltransferase216787 217368 1 582 TM.orf0208 conserved hypothetical protein 217661217350 −2 312 TM.orf0209 microcystin-dependent protein 218298 218855 2558 TM.orf0210 putative microcystin dependent protein 218946 219497 2552 TM.orf0211 tail collar domain-containing protein 219511 220071 1 561TM.orf0212 cytochrome B561 220281 220988 2 708 TM.orf0213 cytochrome C221508 221068 −1 441 TM.orf0214 mcp4 Pmethyl-accepting chemotaxis 222175223602 1 1428 receptor/sensory transducer TM.orf0215 dadA D-amino aciddehydrogenase small subunit 224981 223725 −2 1257 TM.orf0216 dadBputative alanine racemase, catabolic 226230 225064 −1 1167 TM.orf0217Leucine-responsive regulatory protein 226382 226843 3 462 TM.orf0218linC UcpA protein 226937 227698 3 762 TM.orf0219 ydeE transcriptionalregulatory protein 228561 227701 −1 861 TM.orf0220 conservedhypothetical protein 228712 229140 1 429 TM.orf0221 htr5methyl-accepting chemotaxis protein 230871 229171 −1 1701 TM.orf0222ydhC drug resistance transporter, Bcr/CflA subfamily 232207 230993 −31215 TM.orf0223 short-chain dehydrogenase/reductase SDR 233060 232257 −2804 TM.orf0224 2-oxoglutarate and iron-dependent oxygenase 233851 233171−3 681 domain-containing protein 1 TM.orf0225 mmgC putative acyl-CoAdehydrogenase 234531 235685 2 1155 TM.orf0226 lcfB Acyl-CoAsynthetase/AMP-acid ligase II 235682 237250 3 1569 TM.orf0227 samS-adenosylmethionine uptake transporter 238234 237323 −3 912 TM.orf0228Cml2 possible acetyltransferase 238800 238231 −1 570 TM.orf0229chemotaxis sensory transducer 238976 241060 3 2085 TM.orf0230 ileSIsoleucyl-tRNA synthetase 241390 242553 1 1164 TM.orf0231 gcvAtranscriptional regulator 242561 243562 3 1002 TM.orf0232 GDSL-likelipase/acylhydrolase 244208 243579 −2 630 TM.orf0233 ntcA cAMP-bindingprotein - catabolite protein 244888 244205 −3 684 activator andregulatory subunit of cAMP- dependent protein kinase TM.orf0234conserved hypothetical protein 245056 245658 1 603 TM.orf0235 class IIaldolase/adducin family protein 245663 246508 3 846 TM.orf0236Carboxymethylenebutenolidase 246645 247532 2 888 TM.orf0237Monofunctional biosynthetic peptidoglycan 248200 247553 −3 648transglycosylase TM.orf0238 Extracellular serine protease 248526 2552002 6675 TM.orf0239 aromatic compounds catabolic protein 255761 255240 −2522 TM.orf0240 mhqR MarR family transcriptional regulator 255862 2562901 429 TM.orf0241 putative lysine decarboxylase 256878 256297 −1 582TM.orf0242 dctP putative C4 dicarboxylate binding protein 257203 2581921 990 TM.orf0243 siaT Tripartite ATP-independent periplasmic 258225258797 2 573 transporter DctQ component TM.orf0244 siaT TRAPdicarboxylate transporter, DctM subunit 258794 260089 3 1296 TM.orf0245conserved hypothetical protein 260423 260172 −2 252 TM.orf0246 gcvA LysRfamily transcriptional regulator 260662 261588 1 927 TM.orf0247conserved hypothetical protein 261623 262540 3 918 TM.orf0248 cysAsulfate ABC transporter, ATPase subunit 262652 263767 3 1116 TM.orf0249yhaZ conserved hypothetical protein 264557 263808 −2 750 TM.orf0250 yetLregulatory protein, MarR 265045 264554 −3 492 TM.orf0251 bchO ethylferulate-hydrolyzing esterase 266038 265088 −3 951 TM.orf0252 FADdependent oxidoreductase 267555 266038 −1 1518 TM.orf0253 TetR familytranscriptional regulator 267715 268386 1 672 TM.orf0254 ybaN Innermembrane protein ybaN 268811 268383 −2 429 TM.orf0255 katA catalase270504 269020 −1 1485 TM.orf0256 2-polyprenyl-6-methoxyphenolhydroxylase 271822 270644 −3 1179 TM.orf0257 Major facilitatorsuperfamily domain-containing 273069 271819 −1 1251 protein 3 TM.orf0258fyuA TonB-dependent receptor 275101 273074 −3 2028 TM.orf0259 pchRtranscriptional regulator, arac family 275300 276286 3 987 TM.orf0260cation diffusion facilitator family transporter 277241 276246 −2 996TM.orf0261 tcmP O-methyltransferase domain-containing 278163 277291 −1873 protein TM.orf0262 oxyR hydrogen peroxide-inducible genes activator279196 278279 −3 918 TM.orf0263 hypothetical protein 279330 279716 2 387TM.orf0264 proS prolyl-tRNA synthetase 279842 281365 3 1524 TM.orf0265conserved hypothetical protein 281575 282030 1 456 TM.orf0266zinc-finger protein 282178 282603 1 426 TM.orf0267 ohrR transcriptionalregulator, MarR family 282856 283302 1 447 TM.orf0268 ydhCtranscriptional regulator 284050 283334 −3 717 TM.orf0269 class IIaldolase/adducin family protein 284230 285009 1 780 TM.orf0270 gsiB ABCtransporter substrate binding protein 285156 286703 2 1548 TM.orf0271appB oligopeptide ABC transporter 286825 287802 1 978 TM.orf0272 appCABC transporter permease protein 287799 288638 2 840 TM.orf0273 gsiA ABCtransporter ATP-binding protein 288650 290305 3 1656 TM.orf0274 ghrAD-isomer specific 2-hydroxyacid 290302 291273 1 972 dehydrogenaseNAD-binding TM.orf0275 aphA histone deacetylase family protein 291313292341 1 1029 TM.orf0276 aminotransferase 292397 293764 3 1368TM.orf0277 exoT putative succinoglycan transport protein 294288 295754 21467 TM.orf0278 conserved hypothetical protein 296735 297058 3 324TM.orf0279 hypothetical protein 297314 297114 −2 201 TM.orf0280conserved hypothetical protein 298793 298524 −2 270 TM.orf0281 lutRtranscriptional regulator-like 299671 298943 −3 729 TM.orf0282 TRAPdicarboxylate transporter, DctQ subunit 300331 299783 −3 549 TM.orf0283C4-dicarboxylate transporter 301644 300337 −1 1308 TM.orf0284 dctBpossible TrapT family, dctP subunit, C4- 302889 301747 −1 1143dicarboxylate periplasmic binding protein TM.orf0285 aminoglycosidephosphotransferase 304023 302923 −1 1101 TM.orf0286 crt enoyl-CoAhydratase 304211 305059 3 849 TM.orf0287 potI binding-protein dependenttransport system 306353 305532 −2 822 inner membrane protein TM.orf0288potH binding-protein dependent transport system 307310 306372 −2 939inner membrane protein TM.orf0289 potG putrescine transport ATP-bindingprotein PotG 308592 307378 −1 1215 TM.orf0290 potF PotF 309829 308690 −31140 TM.orf0291 puuA glutamine synthetase 311504 310074 −2 1431TM.orf0292 dppF putative oligopeptide ABC transporter (ATP 313018 311990−3 1029 binding protein) TM.orf0293 appD oligopeptide/dipeptide ABCtransporter, 314052 313015 −1 1038 ATPase subunit TM.orf0294 dppCDipeptide transport system permease protein 315002 314124 −2 879 dppCTM.orf0295 binding-protein-dependent transport systems 315975 315019 −1957 inner membrane component TM.orf0296 hbpA extracellularsolute-binding protein 317578 316088 −3 1491 TM.orf0297 peptidase S58DmpA 318724 317585 −3 1140 TM.orf0298 glxR 2-hydroxy-3-oxopropionatereductase 319943 318963 −2 981 TM.orf0299 pyridoxamine 5′-phosphateoxidase family 320088 320702 2 615 protein TM.orf0300 conservedhypothetical protein 321132 321632 2 501 TM.orf0301 proCpyrroline-5-carboxylate reductase 321639 322481 2 843 TM.orf0302conserved hypothetical protein 322663 323526 1 864 TM.orf0303Carboxymethylenebutenolidase 324313 323603 −3 711 TM.orf0304 conservedhypothetical protein 324611 325759 3 1149 TM.orf0305 conservedhypothetical protein 325814 326329 3 516 TM.orf0306 engD PredictedGTPase, probable translation factor 327522 326422 −1 1101 TM.orf0307 pthpeptidyl-tRNA hydrolase 328177 327527 −3 651 TM.orf0308 rplY 50Sribosomal protein L25/general stress 328797 328183 −1 615 protein CtcTM.orf0309 prs ribose-phosphate pyrophosphokinase 329931 328942 −1 990TM.orf0310 conserved hypothetical protein 330683 330189 −2 495TM.orf0311 ade adenine deaminase 332662 330908 −3 1755 TM.orf0312conserved hypothetical protein 333599 332781 −2 819 TM.orf0313 midAconserved hypothetical protein 334801 333623 −3 1179 TM.orf0314Prolipoprotein diacylglyceryl transferase 335616 334798 −1 819TM.orf0315 protein conserved in bacteria 335984 336340 3 357 TM.orf0316putative membrane protein 336346 337263 1 918 TM.orf0317 sdpRtranscriptional regulator, ArsR family 337414 338223 1 810 TM.orf0318rpmB ribosomal protein L28 338503 338814 1 312 TM.orf0319 conservedhypothetical protein 339013 339561 1 549 TM.orf0320 hyfR putativePAS/PAC sensor protein 341505 339562 −1 1944 TM.orf0321 nrtC putativenitrate transporter component, nrtA 341772 343175 2 1404 TM.orf0322 nrtBpossible nitrate transport system permease 343200 344030 2 831 proteinTM.orf0323 cmpD nitrate ABC transporter, ATPase subunits C 344049 3449542 906 and D TM.orf0364 ydhP putative transmembrane efflux precursor382165 383358 1 1194 protein TM.orf0365 conserved hypothetical protein383415 383909 2 495 TM.orf0366 conserved hypothetical protein 383926385074 1 1149 TM.orf0367 52.8 kDa protein in TAR-I ttuC′ 3′region 386612385080 −2 1533 TM.orf0368 tricarboxylic transport 387097 386630 −3 468TM.orf0369 yflP conserved hypothetical protein 388109 387102 −2 1008TM.orf0370 tctD two component transcriptional regulator, 388416 389090 2675 winged helix family TM.orf0371 hypothetical protein 388417 388253 −3165 TM.orf0372 histidine kinase 389101 390489 1 1389 TM.orf0373extracellular solute-binding protein family 1 390486 391586 2 1101TM.orf0374 ubiG 3-demethylubiquinone-9 3-methyltransferase 392398 391607−3 792 TM.orf0375 lysC aspartate kinase 392580 393800 2 1221 TM.orf0376ptsP Signal transduction protein containing GAF 393993 396263 2 2271 andPtsI domains TM.orf0377 conserved hypothetical protein 396535 397770 11236 TM.orf0378 ispG 4-hydroxy-3-methylbut-2-en-1-yl diphosphate 397921399060 1 1140 synthase TM.orf0379 hisS Histidyl-tRNA synthetase, classIIa 399126 400370 2 1245 TM.orf0380 prfA peptide chain release factorRF-1 400381 401469 1 1089 TM.orf0381 hemK HemK family modificationmethylase 401466 402362 2 897 TM.orf0382 conserved hypothetical protein402540 403709 2 1170 TM.orf0383 mal MOSC domain containing protein404581 403811 −3 771 TM.orf0384 clpB ATP-dependent Clp proteaseATP-binding 404822 407419 3 2598 subunit TM.orf0385 yebA Peptidase M23B408960 407533 −1 1428 TM.orf0386 outer membrane autotransporter barrel415506 418865 2 3360 domain-containing protein TM.orf0387 glpRglycerol-3-phosphate transcriptional regulator 418973 419752 3 780protein TM.orf0388 mco Multicopper oxidase family 421157 419772 −2 1386TM.orf0389 pchR PchR 421349 422161 3 813 TM.orf0390 fhuA outer membraneferripyochelin receptor 422306 424453 3 2148 TM.orf0391 membrane protein424458 425720 2 1263 TM.orf0392 PepSY-associated TM helix domain protein425720 426814 3 1095 TM.orf0393 (Acyl-carrier protein) phosphodiesterase427469 426789 −2 681 TM.orf0394 gcvA LysR family transcriptionalregulator 427576 428472 1 897 TM.orf0395 yvbU LysR familytranscriptional regulator 428469 429377 2 909 TM.orf0396 conservedhypothetical protein 429458 430402 3 945 TM.orf0397 ordL FAD dependentoxidoreductase 430538 431851 3 1314 TM.orf0398 TRAP transporter,4TM/12TM fusion protein 433867 431906 −3 1962 TM.orf0399 TRAPtransporter solute receptor TAXI family 435070 434003 −3 1068 proteinTM.orf0400 ybiO MscS Mechanosensitive ion channel 435428 438028 3 2601TM.orf0401 yadH Inner membrane transport permease yadH 438183 438971 2789 TM.orf0402 conserved hypothetical protein 439111 439530 1 420TM.orf0403 ftsK cell divisionFtsK/SpoIIIE 439527 441980 2 2454TM.orf0404 Glutathione peroxidase 442138 442779 1 642 TM.orf0405conserved hypothetical protein 443279 442806 −2 474 TM.orf0406 histonedeacetylase 444178 443276 −3 903 TM.orf0407 Outer membrane protein andrelated 444712 445557 1 846 peptidoglycan-associated lipo proteinTM.orf0408 asmA AsmA protein 445810 447792 1 1983 TM.orf0409 conservedhypothetical protein 447945 448235 2 291 TM.orf0410 hupC cytochrome B561448239 448796 2 558 TM.orf0411 Ion transport 2 domain protein 448999449613 1 615 TM.orf0412 yajO putative Oxidoreductase 450653 449628 −21026 TM.orf0413 protein tyrosine phosphatase 451254 450781 −1 474TM.orf0414 ppc phosphoenolpyruvate carboxylase 451412 454207 3 2796TM.orf0415 dgdR transcriptional regulator, LysR family 454237 455127 1891 TM.orf0416 ndhF putative NADH-quinone oxidoreductase 455220 456689 21470 subunit 5 TM.orf0417 conserved hypothetical protein 456682 459156 12475 TM.orf0418 nitrogen regulatory protein P-II 459203 459559 3 357TM.orf0419 regA transcriptional regulator 460693 459683 −3 1011TM.orf0420 fruB phosphotransferase system, enzyme I 460971 463514 2 2544TM.orf0421 fruK 1-phosphofructokinase 463511 464491 3 981 TM.orf0422fruA PTS system, fructose-specific IIC component 464526 466307 2 1782TM.orf0423 ywoC Amidases related to nicotinamidase 467048 466344 −2 705TM.orf0424 foxA TonB-dependent siderophore receptor 467333 469402 3 2070TM.orf0425 bioA adenosylmethionine--8-amino-7-oxononanoate 471910 470576−3 1335 transaminase TM.orf0426 bioD dithiobiotin synthetase 472545471907 −1 639 TM.orf0427 8-amino-7-oxononanoate synthase BioF 473678472542 −2 1137 TM.orf0428 bioB biotin synthase 474574 473675 −3 900TM.orf0429 ydhC transcriptional regulatory protein 474814 475455 1 642TM.orf0430 ydeP oxidoreductase alpha (molybdopterin) subunit 475529477859 3 2331 TM.orf0431 gno dehydrogenase 478626 477841 −1 786TM.orf0432 conserved hypothetical protein 478688 480127 3 1440TM.orf0433 potA ABC transporter related protein 480236 481363 3 1128TM.orf0434 ABC transporter, periplasmic solute-binding 481456 482583 11128 protein TM.orf0435 potB binding-protein-dependent transport systems482680 483660 1 981 inner membrane component TM.orf0436 potCbinding-protein-dependent transport systems 483657 484469 2 813 innermembrane component TM.orf0437 histidinol dehydrogenase 484499 485821 31323 TM.orf0438 ureR transcriptional regulator, AraC family protein486830 485865 −2 966 TM.orf0439 outer membrane protein W precursor487875 487210 −1 666 TM.orf0440 hemN oxygen-independentcoproporphyrinogen III 488180 489589 3 1410 oxidase TM.orf0441 dhlAhaloalkane dehalogenase 490541 489624 −2 918 TM.orf0442 arginineexporter protein ArgO 491285 490656 −2 630 TM.orf0443 glpDglycerol-3-phosphate dehydrogenase 491733 493265 2 1533 TM.orf0444 glpKglycerol kinase 493445 494989 3 1545 TM.orf0445 TPR repeat-containingprotein 495112 495654 1 543 TM.orf0446 conserved hypothetical protein495786 496427 2 642 TM.orf0447 hypothetical protein 496544 496660 3 117TM.orf0448 kdpA potassium-transporting ATPase subunit A 496804 498504 11701 TM.orf0449 kdpB potassium-translocating P-type ATPase B 498519500567 2 2049 subunit TM.orf0450 kdpC potassium-transporting ATPasesubunit C 500585 501175 3 591 TM.orf0451 kdpD two-component system, OmpRfamily, sensor 501204 503951 2 2748 histidine kinase TM.orf0452 kdpEtwo-component system, response regulator 503973 504704 2 732 TM.orf0453conserved hypothetical protein 505112 504765 −2 348 TM.orf0454 nthBnitrile hydratase beta subunit 505831 505157 −3 675 TM.orf0455 nthAnitrile hydratase alpha subunit 506502 505828 −1 675 TM.orf0456 gcvAtranscriptional regulator 507496 506543 −3 954 TM.orf0457 hypotheticalprotein 508111 507761 −3 351 TM.orf0458 globin 508687 508247 −3 441TM.orf0459 ErfK/YbiS/YcfS/YnhG 508807 509469 1 663 TM.orf0460 putativelipoprotein 509478 510302 2 825 TM.orf0461 arsC protein tyrosinephosphatase 510431 510949 3 519 TM.orf0462 pdhR GntR domain-containingprotein 511748 510972 −2 777 TM.orf0463 FMN-dependent alpha-hydroxy acid511872 513029 2 1158 dehydrogenase TM.orf0464 isocitrate lyase andphosphorylmutase 513155 514018 3 864 TM.orf0465 hypothetical protein514015 514371 1 357 TM.orf0466 ABC transporter substrate-binding protein514469 515752 3 1284 TM.orf0467 conserved hypothetical protein 517165516215 −3 951 TM.orf0468 peptidase 517201 517578 1 378 TM.orf0469 qseBtwo-component transcriptional regulator FeuP, 517575 518240 2 666 wingedhelix family TM.orf0470 phoQ two-component sensor histidine kinase518237 519682 3 1446 TM.orf0471 iorB Twin-arginine translocation pathwaysignal 521914 519695 −3 2220 TM.orf0472 iorA putative aldehydedehydrogenase subunit III 522370 521918 −3 453 TM.orf0473transcriptional regulator, AraC family 522591 523544 2 954 TM.orf0474lcfA putative ligase 525113 523548 −2 1566 TM.orf0475 Extracellularligand-binding receptor 526400 525171 −2 1230 TM.orf0476 petPtranscriptional regulator 526590 527138 2 549 TM.orf0477 conservedhypothetical protein 527847 527146 −1 702 TM.orf0478 panCpantoate--beta-alanine ligase 528817 527900 −3 918 TM.orf0479hypothetical protein 529043 530308 3 1266 TM.orf0480 hypotheticalprotein 530716 530312 −3 405 TM.orf0481 ribH6,7-dimethyl-8-ribityllumazine synthase 531291 530773 −1 519 TM.orf0482gmhB Histidinol phosphatase and related 531698 532249 3 552 phosphatasesTM.orf0483 paaG enoyl-CoA hydratase 533046 532252 −1 795 TM.orf0484radical SAM domain-containing protein 533444 534793 3 1350 TM.orf0485bchE Radical SAM domain protein 534908 536443 3 1536 TM.orf0486AMP-dependent synthetase and ligase 536464 538260 1 1797 TM.orf0487 eryApolyketide synthase 538257 545873 2 7617 TM.orf0488 thioesterasedomain-containing protein 545926 546360 1 435 TM.orf0489 acpP acylcarrier protein 546431 546682 3 252 TM.orf0490 fabF3-oxoacyl-[acyl-carrier-protein] synthase II 546700 548034 1 1335(Beta-ketoacyl-ACP synthase II) (KAS II) TM.orf0491 phage tail Collar548247 548765 2 519 TM.orf0492 Tail Collar domain protein 548785 5493331 549 TM.orf0493 phage tail Collar 549347 549880 3 534 TM.orf0494Nitrilase/cyanide hydratase and apolipoprotein 551667 549940 −1 1728N-acyltransferase TM.orf0495 braG putative branched-chain amino acid ABC552409 551714 −3 696 transporter, ATP-binding protein TM.orf0496 braFbranched chain amino acid ABC transporter 553173 552409 −1 765ATP-binding protein TM.orf0497 IivM putative branched-chain amino acidABC 554282 553170 −2 1113 transporter, permease protein TM.orf0498 braDputative permease component of ABC 555185 554292 −2 894 transporterTM.orf0499 amiC Aliphatic amidase expression-regulating 556529 555276 −21254 protein TM.orf0500 amiR two component response regulator 557586556978 −1 609 TM.orf0501 amiC two component sensor kinase 558743 557613−2 1131 TM.orf0502 major facilitator transporter 559050 560237 2 1188TM.orf0503 exoD exopolysaccharide synthesis, ExoD 560901 560248 −1 654TM.orf0504 ABC-type transport system, periplasmic 561238 561855 1 618component TM.orf0505 membrane protein 561833 562960 3 1128 TM.orf0506dokA Signal transduction histidine kinase 563084 564592 3 1509TM.orf0507 putative phosphohistidine phosphatase, SixA 564654 565271 2618 TM.orf0508 crtK TspO and MBR like protein 565318 565920 1 603TM.orf0509 dhkA PAS fold family 565990 567486 1 1497 TM.orf0510 GCDHacyl-CoA dehydrogenase domain-containing 568693 567503 −3 1191 proteinTM.orf0511 nitroreductase 568851 569519 2 669 TM.orf0512Leu/Ile/Val-binding protein 570792 569566 −1 1227 TM.orf0513 lcfBAMP-dependent synthetase and ligase 571032 572663 2 1632 TM.orf0514 ybcLputative efflux transporter 573873 572689 −1 1185 TM.orf0515 nahRtranscriptional regulator, LysR family 574012 574938 1 927 TM.orf0516ybaK ybaK/ebsC protein 575000 575491 3 492 TM.orf0517Phospholipase/Carboxylesterase 576117 575497 −1 621 TM.orf0518thioredoxin reductase 577220 576114 −2 1107 TM.orf0519 FMO5monooxygenase 578828 577506 −2 1323 TM.orf0520 NAD-dependentepimerase/dehydratase 579816 578839 −1 978 TM.orf0521 acnR putativeTetR-family transcriptional regulator 579957 580628 2 672 TM.orf0522acyl-CoA dehydrogenase domain protein 580669 581832 1 1164 TM.orf0523yfdE L-carnitine dehydratase/bile acid-inducible 581847 582971 2 1125protein F TM.orf0524 htd2 Hydroxyacyl-thioester dehydratase type 2582968 583837 3 870 TM.orf0525 yciC cobalamin synthesis protein P47K583974 585140 2 1167 CH42_BURCC RecName: Full = GTP TM.orf0526cyclohydrolase folE2 2 585216 586118 2 903 TM.orf0527 carbonicanhydrase/acetyltransferase 586156 586716 1 561 isoleucine patchsuperfamily TM.orf0528 thrS threonyl-tRNA synthetase 586723 588711 11989 TM.orf0529 allB dihydroorotase 588708 590063 2 1356 TM.orf0530 lmrAregulatory protein, TetR 590631 590041 −1 591 TM.orf0531 pksS cytochromeP450 590886 592121 2 1236 TM.orf0532 Enoyl-CoA hydratase/isomerase592172 593083 3 912 TM.orf0533 alanyl-tRNA synthetase-like protein593148 593792 2 645 TM.orf0534 hypothetical protein 593789 593926 3 138TM.orf0535 metal dependent phosphohydrolase 594581 593940 −2 642TM.orf0536 AraC family transcriptional regulator 594749 595756 3 1008TM.orf0537 phnO acetyltransferase protein 596183 595719 −2 465TM.orf0538 thiolase 597626 596370 −2 1257 TM.orf0539 pabpara-aminobenzoate synthase 597796 599931 1 2136 TM.orf0540 conservedhypothetical protein 600061 600906 1 846 TM.orf0541 qor alcoholdehydrogenase 602007 601015 −1 993 TM.orf0542 mauR transcriptionalregulator protein 602150 603052 3 903 TM.orf0543 catA putativedioxygenase 603116 603706 3 591 TM.orf0544 yeaM AraC familytranscriptional regulator 604467 603676 −1 792 TM.orf0545 conservedhypothetical protein 604608 605018 2 411 TM.orf0546 iorA indolepyruvateferredoxin oxidoreductase 607828 605153 −3 2676 subunit alpha/betaTM.orf0547 ADH4 putative iron-containing alcohol 609219 610397 2 1179dehydrogenase TM.orf0548 yncG glutathione S-transferasedomain-containing 610399 611100 1 702 protein TM.orf0549 thioesterasefamily protein 611097 611534 2 438 TM.orf0550 conserved hypotheticalprotein 611534 611995 3 462 TM.orf0551 lcfB putative AMP-dependentsynthetase and 612106 613716 1 1611 ligase TM.orf0552 chemotaxistransducer 613861 615543 1 1683 TM.orf0553 conserved hypotheticalprotein 616401 615544 −1 858 TM.orf0554 acoA Thiaminepyrophosphate-dependent 616817 617848 3 1032 dehydrogenase, E1 componentalpha subunit TM.orf0555 putative pyruvate dehydrogenase E1 beta 617854618828 1 975 subunit TM.orf0556 yiaO 2,3-diketo-L-gulonate-bindingperiplasmic 618991 620031 1 1041 protein yiaO TM.orf0557 yiaM TripartiteATP-independent periplasmic 620150 620704 3 555 transporter DctQcomponent TM.orf0558 putative membrane permease 620762 622084 3 1323TM.orf0559 ilvB putative acetolactate synthase large subunit 622226623998 3 1773 TM.orf0560 conserved hypothetical protein 624099 624686 2588 TM.orf0561 prfC peptide chain release factor 3 626404 624803 −3 1602TM.orf0562 virulence-associated protein VapB-like protein 626680 6269701 291 TM.orf0563 hydrolase-like protein protein of the 627843 627025 −1819 alpha/beta-hydrolase fold family TM.orf0564 conserved hypotheticalprotein 627993 628298 2 306 TM.orf0565 exoI nuclease (SNase-like) 628791628339 −1 453 TM.orf0566 katG catalase/peroxidase HPI 634943 632781 −22163 TM.orf0567 cysteine desulfuration protein SufE 635136 635567 2 432TM.orf0568 class II aldolase/adducin domain protein 635598 636389 2 792TM.orf0569 OsmC family protein 636514 636900 1 387 TM.orf0570 Amidase636948 638387 2 1440 TM.orf0571 conserved hypothetical protein 638855640081 3 1227 TM.orf0572 hypothetical protein 640122 640562 2 441TM.orf0573 paaA phenylacetic acid degradation protein (similar 640657641412 1 756 to paaA) TM.orf0574 soj chromosome partitioning protein642261 643097 2 837 TM.orf0575 conserved hypothetical protein 643191643934 2 744 TM.orf0576 Heat shock protein DnaJ-like protein 643951644727 1 777 TM.orf0577 exoN UTP--glucose-1-phosphateuridylyltransferase 645077 645976 3 900 TM.orf0578 rkpK putative UDPglucose dehydrogenase 646078 647397 1 1320 TM.orf0579 exoCphosphomannomutase/phosphoglucomutase 647463 648872 2 1410 TM.orf0580yjbE putative transmembrane protein 649638 648958 −1 681 TM.orf0581putative methyl-accepting chemotaxis protein 649939 651363 1 1425TM.orf0582 ribokinase 652335 651364 −1 972 TM.orf0583 hypotheticalprotein 652572 652417 −1 156 TM.orf0584 dacF D-alanyl-D-alaninecarboxypeptidase 654331 652757 −3 1575 TM.orf0585 conserved hypotheticalprotein 654712 655467 1 756 TM.orf0586 conserved hypothetical protein655702 656541 1 840 TM.orf0587 clpS ATP-dependent Clp protease adaptorprotein 656820 657164 2 345 ClpS TM.orf0588 ATP-dependent Clp proteaseATP-binding 657164 659569 3 2406 subunit ClpA TM.orf0589 conservedhypothetical protein 661060 660392 −3 669 TM.orf0590 hypotheticalprotein 661403 661134 −2 270 TM.orf0591 hypothetical protein 661698661420 −1 279 TM.orf0592 conserved hypothetical protein 662968 661796 −31173 TM.orf0593 conserved hypothetical protein 663406 663008 −3 399TM.orf0594 conserved hypothetical protein 663738 663403 −1 336TM.orf0595 dhbF syringomycin synthetase 667650 663781 −1 3870 TM.orf0596conserved hypothetical protein 668332 667784 −3 549 TM.orf0597 Chain A,Crystal Structure Of Cmls, A Flavin- 670077 668353 −1 1725 DependentHalogenase TM.orf0598 putative acetyltransferase 670754 670074 −2 681TM.orf0599 lcfB Long-chain-fatty-acid--CoA ligase 672127 670751 −3 1377TM.orf0600 fabG short-chain dehydrogenase/reductase SDR 672963 672124 −1840 TM.orf0601 htpX putative protease htpX homolog 674367 673189 −1 1179TM.orf0602 conserved hypothetical protein 675506 674370 −2 1137TM.orf0603 ilvI acetolactate synthase 2 catalytic subunit 677353 675629−3 1725 TM.orf0604 translation initiation inhibitor 677895 677428 −1 468TM.orf0605 dhaS betaine-aldehyde dehydrogenase 679419 677941 −1 1479TM.orf0606 nemA GTN reductase 680812 679697 −3 1116 TM.orf0607 lrpleucine-responsive regulatory protein 681490 681008 −3 483 TM.orf0608putA delta-1-pyrroline-5-carboxylate dehydrogenase 681671 684853 3 3183TM.orf0609 metA Homoserine O-succinyltransferase 685094 686035 3 942TM.orf0610 dhaR transcriptional regulator, TetR family 686626 686042 −3585 TM.orf0611 ybfB major facilitator superfamily MFS-1 686797 688068 11272 TM.orf0612 PRX1 BcpB protein 688671 688087 −1 585 TM.orf0613 aminoacid transporter LysE 689327 688668 −2 660 TM.orf0614 conservedhypothetical protein 690148 689324 −3 825 TM.orf0615 cmoAmethyltransferase domain protein 690891 690145 −1 747 TM.orf0616 ydeGMFS-type transporter ydeG 692297 691077 −2 1221 TM.orf0617inosine-uridine preferring nucleoside hydrolase 692498 693520 3 1023family protein TM.orf0618 hlyB putative chemotaxis protein 693705 6959782 2274 TM.orf0619 hypothetical protein 696163 696546 1 384 TM.orf0620bfr bacterioferritin 697119 696637 −1 483 TM.orf0621 hypotheticalprotein 697540 697331 −3 210 TM.orf0622 acetyl-CoA synthetase 699297697648 −1 1650 TM.orf0623 ydbL conserved hypothetical protein 699801699409 −1 393 TM.orf0624 ynbE conserved hypothetical protein 699995699801 −2 195 TM.orf0625 conserved hypothetical protein 702253 700028 −32226 TM.orf0626 prtG calcium binding hemolysin protein 704409 702337 −12073 TM.orf0627 cya calcium binding hemolysin protein 707645 704406 −23240 TM.orf0628 hbd 3-hydroxybutyryl-CoA dehydrogenase 708626 707766 −2861 TM.orf0629 acyl-CoA dehydrogenase family protein 710498 708687 −21812 TM.orf0630 yngJ acyl-CoA dehydrogenase 711807 710659 −1 1149TM.orf0631 yngJ pimeloyl-CoA dehydrogenase, large subunit 713033 711837−2 1197 TM.orf0632 peroxisomal bifunctional enzyme 715404 713272 −1 2133TM.orf0633 IivF ABC transporter related protein 716251 715547 −3 705TM.orf0634 braF ABC transporter related protein 717000 716248 −1 753TM.orf0635 IivM inner-membrane translocator 718319 717000 −2 1320TM.orf0636 IivH inner-membrane translocator 719266 718316 −3 951TM.orf0637 ABC transporter, periplasmic branched chain 720794 719529 −21266 amino acid binding protein TM.orf0638 enoyl-CoA hydratase 721169721630 3 462 TM.orf0639 alkK Acyl-CoA synthetases (AMP-forming)/AMP-723334 721679 −3 1656 acid ligase II TM.orf0640 stationary-phasesurvival protein SurE 724368 723562 −1 807 TM.orf0641 cpnA dehydrogenase724537 725313 1 777 TM.orf0642 short-chain dehydrogenase/reductase SDR725393 726169 3 777 TM.orf0643 aminoglycoside phosphotransferase 726257727384 3 1128 TM.orf0644 conserved hypothetical protein 729404 727473 −21932 TM.orf0645 Helix-turn-helix motif 730001 729531 −2 471 TM.orf0646putative acyl-CoA dehydrogenase 731374 730163 −3 1212 TM.orf0647 acnRTetR family transcriptional regulator 731751 732479 2 729 TM.orf0648oxidoreductase, zinc-binding dehydrogenase 732536 733510 3 975 familyTM.orf0649 methyl-accepting chemotaxis sensory 733792 735945 1 2154transducer TM.orf0650 conserved hypothetical protein 736107 736688 2 582TM.orf0651 cutL carbon-monoxide dehydrogenase (acceptor) 739092 736771−1 2322 TM.orf0652 chromate transporter 739344 740543 2 1200 TM.orf0653transcriptional regulatory protein ZraR 741144 740554 −1 591 TM.orf0654rpoH RNA polymerase sigma-32 factor 741640 742587 1 948 TM.orf0655soluble lytic murein transglycosylase and 743595 742660 −1 936 relatedregulatory protein TM.orf0656 hypothetical protein 744009 743890 −1 120TM.orf0657 phhA phenylalanine 4-monooxygenase 745351 744503 −3 849TM.orf0658 pat GCN5-related N-acetyltransferase 746049 745474 −1 576TM.orf0659 N-formylglutamate amidohydrolase 747143 746187 −2 957TM.orf0660 conserved hypothetical protein 747698 748045 3 348 TM.orf0661conserved hypothetical protein 748108 748539 1 432 TM.orf0662 ybiCmalate dehydrogenase 749609 748530 −2 1080 TM.orf0663 HTH-typetranscriptional regulator in instable 749726 750433 3 708 DNA locusTM.orf0664 dctP c4-dicarboxylate-binding periplasmic protein 750531751577 2 1047 TM.orf0665 Tripartite ATP-independent periplasmic 751608752219 2 612 transporter DctQ component TM.orf0666 siaT C4-dicarboxylateTRAP-T family tripartite ATP- 752216 753484 3 1269 independentperiplasmic transporter, membrane protein, large subunit TM.orf0667conserved hypothetical protein 754396 753521 −3 876 TM.orf0668hypothetical protein 754660 755205 1 546 TM.orf0669 sodium/hydrogenexchanger family protein 755322 757847 2 2526 TM.orf0670 conservedhypothetical protein 758235 757852 −1 384 TM.orf0671 conservedhypothetical protein 758543 758232 −2 312 TM.orf0672 codA cytosinedeaminase 759953 758661 −2 1293 TM.orf0673 yufQ inner-membranetranslocator 760898 759957 −2 942 TM.orf0674 yufP inner-membranetranslocator 761966 760917 −2 1050 TM.orf0675 yufO ABC transportercomponent 763503 761956 −1 1548 TM.orf0676 med Transcriptional activatorprotein med 764695 763700 −3 996 TM.orf0677 ytxM Putative esterase ytxM765825 764917 −1 909 TM.orf0678 apl dedA family protein 766513 765920 −3594 TM.orf0679 Arylformamidase 766698 767585 2 888 TM.orf0680 hldE rfaEbifunctional protein 769104 767590 −1 1515 TM.orf0681 gmhAPhosphoheptose isomerase 769759 769145 −3 615 TM.orf0682 cupin 2domain-containing protein 769946 770377 3 432 TM.orf0683 hldDnucleoside-diphosphate-sugar epimerase 771374 770388 −2 987 TM.orf0684hypothetical protein 771640 771888 1 249 TM.orf0685 gfcB lipoproteingfcB 772654 771977 −3 678 TM.orf0686 sulfate adenylyltransferase subunit2 sulfate 772921 773724 1 804 adenylatetransferase sat ATP-sulfurylasesmall subunit TM.orf0687 tuf sulfate adenylyltransferase, large subunit773744 774697 3 954 TM.orf0688 cysC NodQ bifunctional enzyme 774694775674 1 981 TM.orf0689 lcfA 4-coumarate-CoA ligase 777276 775735 −11542 TM.orf0690 peroxisomal 2,4-dienoyl-CoA reductase 778127 777318 −2810 TM.orf0691 thiolase 779269 778124 −3 1146 TM.orf0692 DUF35 779685779266 −1 420 TM.orf0693 peroxisomal multifunctional enzyme type 2780596 779682 −2 915 TM.orf0694 alpha-methylacyl-CoA racemase 781834780686 −3 1149 TM.orf0695 slyA transcriptional regulator, MarR family782310 781861 −1 450 TM.orf0696 FOX2 peroxisomal multifunctional enzymetype 2 783339 782434 −1 906 TM.orf0697 acyl-CoA dehydrogenase 783507784658 2 1152 TM.orf0698 lcfA acyl-CoA synthase 784655 786238 3 1584TM.orf0699 enoyl-CoA hydratase/isomerase 786235 787038 1 804 TM.orf0700siaT trap dicarboxylate transporter, dctm subunit 788453 787113 −2 1341TM.orf0701 tripartite ATP-independent periplasmic 788963 788457 −2 507transporter DctQ TM.orf0702 dctB Bacterial extracellular solute-bindingprotein, 790096 789038 −3 1059 family 7 TM.orf0703 cysW Sulfate ABCtransporter permease CysW 791154 790300 −1 855 TM.orf0704 cysT sulfatetransport system permease protein 792007 791159 −3 849 TM.orf0705 sbpsulfate ABC transporter, periplasmic sulfate- 793059 792013 −1 1047binding protein TM.orf0706 MUM4 (MUCILAGE-MODIFIED 4); UDP-4- 794335793313 −3 1023 keto-6-deoxy-glucose-3,5-epimerase/UDP-4-keto-rhamnose-4-keto-reductase/UDP-L- rhamnose synthase/UDP-glucose 4,6-dehydratase/catalytic TM.orf0707 hldD NAD-dependentepimerase/dehydratase 795138 794335 −1 804 TM.orf0708 rfbPundecaprenyl-phosphate galactose 795619 796302 1 684 phosphotransferaseTM.orf0709 capI NAD-dependent epimerase/dehydratase 796462 797448 1 987TM.orf0710 capL UDP-N-acetyl-D-mannosaminuronate 797455 798807 1 1353dehydrogenase TM.orf0711 kpsD polysaccharide biosynthesis/export protein798962 801733 3 2772 TM.orf0712 gfcD lipoprotein gfcD 801746 804310 32565 TM.orf0713 rfbF glucose-1-phosphate cytidylyltransferase 804589805395 1 807 TM.orf0714 rfbN glycosyl transferase, group 2 familyprotein 805747 806772 1 1026 TM.orf0715 algA GDP-mannosepyrophosphorylase EpsQ 806941 808362 1 1422 TM.orf0716 hypotheticalprotein 809191 810693 1 1503 TM.orf0717 transposase, IS4 811033 810656−3 378 TM.orf0718 transposase 811329 811030 −1 300 TM.orf0719transposase 812031 811762 −1 270 TM.orf0720 putative transposase number1 of insertion 812834 812286 −2 549 sequence NGRIS-2b TM.orf0721nitroreductase 813882 814241 2 360 TM.orf0722 glycosyltransferase 814291815379 1 1089 TM.orf0723 conserved hypothetical protein 815651 815971 3321 TM.orf0724 phnA putative polybetahydroxybutyrate synthesis 816051816371 2 321 PhnA protein TM.orf0725 conserved hypothetical protein816451 817203 1 753 TM.orf0726 transcriptional regulator, TetR familyprotein 817819 817235 −3 585 TM.orf0727 yxaH putative membrane protein817946 819001 3 1056 TM.orf0728 bioY putative biotin transporter bioY819599 819009 −2 591 TM.orf0729 yqjI transcriptional regulator,PadR-like family 819792 820448 2 657 TM.orf0730 conserved hypotheticalprotein 820445 820891 3 447 TM.orf0731 ynfM Inner membrane transportprotein ynfM 822157 820892 −3 1266 TM.orf0732 alsR transcriptionalregulator, LysR family 822266 823165 3 900 TM.orf0733 glyoxalase familyprotein 823242 823730 2 489 TM.orf0734 ybaA conserved hypotheticalprotein 823730 824089 3 360 TM.orf0735 ttr Putative acetyltransferaseprotein 824625 824080 −1 546 TM.orf0736 XRE family transcriptionalregulator 825205 824636 −3 570 TM.orf0737 pecS transcriptional regulatorprotein 825751 825248 −3 504 TM.orf0738 pecM putative transmembraneprotein 825858 826748 2 891 TM.orf0739 yfmJ NADP-dependent dehydrogenase826767 827801 2 1035 TM.orf0740 ywfM transporter ywfM 828741 827788 −1954 TM.orf0741 ornithine cyclodeaminase/mu-crystallin 829797 828844 −1954 TM.orf0742 conserved hypothetical protein 830365 829883 −3 483TM.orf0743 hosA MarR family transcriptional regulator 830469 830942 2474 TM.orf0744 conserved hypothetical protein 831065 832447 3 1383TM.orf0745 arsenate reductase and related 832953 832576 −1 378TM.orf0746 peptidase M14, carboxypeptidase A 834152 832953 −2 1200TM.orf0747 metC Cys/Met metabolism pyridoxal-phosphate- 834281 835465 31185 dependent enzymes TM.orf0748 rhlE DEAD/DEAH box helicasedomain-containing 836034 837620 2 1587 protein TM.orf0749 bdlAMethyl-accepting chemotaxis protein 839419 837737 −3 1683 TM.orf0750phenazine biosynthesis protein PhzF family 840341 839514 −2 828TM.orf0751 Multidrug resistance protein B 841645 840386 −3 1260TM.orf0752 peptidase C45, acyl-coenzyme A: 6- 842814 841633 −1 1182aminopenicillanic acid acyl-transferase TM.orf0753 lysX S6 modificationenzyme RimK 843734 842811 −2 924 TM.orf0754 norM MATE efflux familyprotein 845343 843844 −1 1500 TM.orf0755 conserved hypothetical protein845638 846540 1 903 TM.orf0756 conserved hypothetical protein 846561847322 2 762 TM.orf0757 GGDEF domain containing protein 847396 848274 1879 TM.orf0758 diguanylate cyclase with PAS/PAC sensor 848280 848630 2351 TM.orf0759 hypothetical protein 848932 849105 1 174 TM.orf0760 leuA2-isopropylmalate synthase 849191 850897 3 1707 TM.orf0761 mreB rodshape-determining protein MreB and 851190 852230 2 1041 related proteinsTM.orf0762 mreC Rod shape-determining protein MreC 852430 853335 1 906TM.orf0763 Cell shape-determining protein 853352 853867 3 516 TM.orf0764mrdA penicillin binding protein 2 853953 855959 2 2007 TM.orf0765 mrdBBacterial cell division membrane protein 855956 857107 3 1152 TM.orf0766hypothetical protein 857429 857650 3 222 TM.orf0767 pldC phospholipaseD/transphosphatidylase 859194 857668 −1 1527 TM.orf0768 yjaBGCN5-related N-acetyltransferase 859390 859845 1 456 TM.orf0769 rutFflavin reductase domain-containing protein 860411 859860 −2 552TM.orf0770 yhgD transcriptional regulator, TetR family 860595 861254 2660 TM.orf0771 eutG maleylacetate reductase 862448 861276 −2 1173TM.orf0772 conserved hypothetical protein 862698 863018 2 321 TM.orf0773Bbp2 863033 863611 3 579 TM.orf0774 hypothetical protein 863608 863973 1366 TM.orf0775 C repressor protein 864710 863964 −2 747 TM.orf0776conserved hypothetical protein 864876 865304 2 429 TM.orf0777 conservedhypothetical protein 865831 865328 −3 504 TM.orf0778 hypotheticalprotein 866147 865857 −2 291 TM.orf0779 exoI putative nuclease 867100866357 −3 744 TM.orf0780 thrS threonyl-tRNA synthetase 867419 869302 31884 TM.orf0781 Outer membrane lipoprotein-like 869483 870010 3 528TM.orf0782 conserved hypothetical protein 870055 870642 1 588 TM.orf0783filamentation induced by cAMP protein Fic 870812 871078 3 267 TM.orf0784hypothetical protein 871209 872570 2 1362 TM.orf0785 hypotheticalprotein 872707 873117 1 411 TM.orf0786 arsB membrane anion transportprotein 873236 874462 3 1227 TM.orf0787 conserved hypothetical protein874551 876017 2 1467 TM.orf0788 transcriptional regulator 876446 876036−2 411 TM.orf0789 cry2 Deoxyribodipyrimidine photolyase 876900 878381 21482 TM.orf0790 icc metallophosphoesterase 878427 879224 2 798TM.orf0791 glxA transcriptional regulator, AraC family 880193 879255 −2939 TM.orf0792 ThiJ/PfpI family protein 880283 880981 3 699 TM.orf0793extracellular ligand-binding receptor 882269 880995 −2 1275 TM.orf0794oxidoreductase, short chain 883289 882483 −2 807 dehydrogenase/reductasefamily TM.orf0795 Short-chain dehydrogenase/reductase SDR 883557 8843152 759 TM.orf0796 Rhomboid family protein 884613 885104 2 492 TM.orf0797conserved hypothetical protein 885466 885122 −3 345 TM.orf0798 conservedhypothetical protein 885588 886211 2 624 TM.orf0799 acetyltransferase886436 886909 3 474 TM.orf0800 possible periplasmic substrate-bindingprotein, 887349 888662 2 1314 ABC-type amino acid ABC transporterTM.orf0801 braD inner-membrane translocator 888777 889673 2 897TM.orf0802 inner-membrane translocator 889688 890713 3 1026 TM.orf0803IivG ABC transporter related protein 890710 891459 1 750 TM.orf0804 IivFABC transporter related protein 891479 892138 3 660 TM.orf0805 lcfBputative acyl coenzyme A synthetase, long- 892181 893356 3 1176chain-fatty-acid--CoA ligase TM.orf0806 istB IstB domain proteinATP-binding protein 894205 893414 −3 792 TM.orf0807 istA Integrase,catalytic region 895700 894192 −2 1509 TM.orf0808 intZ site-specificrecombinase, phage integrase 897748 895811 −3 1938 family TM.orf0809hypothetical protein 898105 897965 −3 141 TM.orf0810 YGGT family protein898781 898464 −2 318 TM.orf0811 Na+/solute symporter (Ssf family) 900627898861 −1 1767 TM.orf0812 membrane protein 900934 900641 −3 294TM.orf0813 hypothetical protein 901151 901759 3 609 TM.orf0814 dnaQ DNApolymerase III, epsilon subunit 901740 903149 2 1410 TM.orf0815Predicted signal-transduction protein 903243 904697 2 1455 containingcAMP-binding and CBS domains TM.orf0816 pldB alpha/beta hydrolase foldprotein 904706 905701 3 996 TM.orf0817 short-chaindehydrogenase/reductase SDR 906481 905702 −3 780 TM.orf0818 ybfI AraCfamily transcriptional regulator 907367 906504 −2 864 TM.orf0819 caiEphenylacetic acid degradation protein PaaY 907543 908148 1 606TM.orf0820 putative ABC transporter ATP-binding protein 908308 909990 11683 TM.orf0821 virulence-associated protein D 910204 910410 1 207TM.orf0822 mlaC toluene tolerance transporter 911169 910477 −1 693TM.orf0823 Surface lipoprotein 912117 911266 −1 852 TM.orf0824transcriptional regulator 912352 913419 1 1068 TM.orf0825 metF5,10-methylenetetrahydrofolate reductase 913416 914360 2 945 TM.orf0826metH 5-methyltetrahydrofolate--homocysteine 914413 917922 1 3510methyltransferase TM.orf0827 NAD(P)(+) transhydrogenase (AB-specific)918395 919534 3 1140 TM.orf0828 NAD(P)(+) transhydrogenase (AB-specific)919531 919950 1 420 TM.orf0829 pntB NAD(P) transhydrogenase, betasubunit 919961 921364 3 1404 TM.orf0830 tauD putativealpha-ketoglutarate-dependent taurine 922702 921779 −3 924 dioxygenaseTM.orf0831 Transcriptional regulator, AraC family protein 922852 9239161 1065 TM.orf0832 conserved hypothetical protein 924188 923874 −2 315TM.orf0833 30S ribosomal protein S21 924597 924391 −1 207 TM.orf0834transcriptional activator, TenA family 924910 925581 1 672 TM.orf0835COQ9 Ubiquinone biosynthesis protein 925664 926437 3 774 TM.orf0836 purKphosphoribosylaminoimidazole carboxylase 927569 926454 −2 1116 ATPasesubunit TM.orf0837 purE phosphoribosylcarboxyaminoimidazole mutase928135 927611 −3 525 TM.orf0838 conserved hypothetical protein 928319928906 3 588 TM.orf0839 sulfatase 929009 930739 3 1731 TM.orf08402-nitropropane dioxygenase 930795 931772 2 978 TM.orf0841 putativetranscriptional regulator family 931811 932302 3 492 TM.orf0842conserved hypothetical protein 932637 932819 2 183 TM.orf0843 ubiXaromatic acid decarboxylase 933975 933316 −1 660 TM.orf0844 conservedhypothetical protein 934076 934243 3 168 TM.orf0845 alkK Acyl-CoAsynthetase (AMP-forming)/AMP-acid 936065 934428 −2 1638 ligase IITM.orf0846 acsA AMP-dependent synthetase and ligase 938073 936157 −11917 TM.orf0847 hypothetical protein 938444 938578 3 135 TM.orf0848 ybfBMFS-type transporter ybfB 938618 939883 3 1266 TM.orf0849 AAA ATPase939969 942092 2 2124 TM.orf0850 conserved hypothetical protein 942089943507 3 1419 TM.orf0851 conserved hypothetical protein 944176 943583 −3594 TM.orf0852 quinone oxidoreductase 945380 944355 −2 1026 TM.orf0853rnz beta-lactamase superfamily hydrolase 946345 945536 −3 810 TM.orf0854rnz beta-lactamase domain protein 946664 947518 3 855 TM.orf0855 cyclasefamily protein 948746 947757 −2 990 TM.orf0856 3-hydroxybutyratedehydrogenase 949225 950004 1 780 TM.orf0857 alpha/beta hydrolase fold950054 950875 3 822 TM.orf0858 putative excisonase protein 950962 9514051 444 TM.orf0859 rutR TetR family transcriptional regulator 952074951400 −1 675 TM.orf0860 yufQ inner-membrane translocator 953023 952079−3 945 TM.orf0861 yufP permease protein of sugar ABC transporter 954108953020 −1 1089 TM.orf0862 yufO ABC transporter, ATP-binding protein955661 954105 −2 1557 TM.orf0863 med Basic membrane lipoprotein 956701955721 −3 981 TM.orf0864 putative iron/ascorbate oxidoreductase 956986958041 1 1056 TM.orf0865 sulfite reductase 959499 958093 −1 1407TM.orf0866 hypothetical protein 959937 959536 −1 402 TM.orf0867 putativecache sensor protein 960487 959996 −3 492 TM.orf0868 glycinebetaine/proline transport system 961483 960599 −3 885 substrate-bindingprotein TM.orf0869 binding-protein-dependent transport systems 962426961581 −2 846 inner membrane component TM.orf0870 proV glycinebetaine/L-proline ABC transporter, 963712 962426 −3 1287 ATPase subunitTM.orf0871 putative addiction module antidote protein, 964193 964423 3231 CopG/Arc/MetJ family TM.orf0872 hspB small heat shock protein 965045964605 −2 441 TM.orf0873 ybfL transposase, is4 family 965331 966446 21116 TM.orf0874 rpmE large subunit ribosomal protein L31 966850 966629−3 222 TM.orf0875 ytxE OmpA/MotB domain protein 968218 966944 −3 1275TM.orf0876 conserved hypothetical protein 969468 968293 −1 1176TM.orf0877 hypothetical protein 969997 969587 −3 411 TM.orf0878 suhBfructose-1 6-bisphosphatase 971114 970272 −2 843 TM.orf0879 efpelongation factor P 971848 971282 −3 567 TM.orf0880 thiEthiamine-phosphate pyrophosphorylase 972731 972063 −2 669 TM.orf0881fbaB fructose-bisphosphate aldolase 973648 972728 −3 921 TM.orf0882hypothetical protein 974192 973854 −2 339 TM.orf0883 pgkphosphoglycerate kinase 975422 974226 −2 1197 TM.orf0884 gapglyceraldehyde-3-phosphate dehydrogenase 976536 975532 −1 1005TM.orf0885 hypothetical protein 976579 976710 1 132 TM.orf0886 cbbTtransketolase 978670 976667 −3 2004 TM.orf0887 glnT putative amino acidcarrier 979078 980508 1 1431 protein(sodium/alanine symporter)TM.orf0888 aspA aspartate ammonia-lyase 980586 981959 2 1374 TM.orf0889ansA cytoplasmic asparaginase I 982881 982036 −1 846 TM.orf0890Aspartate racemase 983627 982881 −2 747 TM.orf0891 putativetranscriptional regulator 983797 984603 1 807 TM.orf0892 hypotheticalprotein 984949 985254 1 306 TM.orf0893 conserved hypothetical protein985283 985633 3 351 TM.orf0894 5-formyltetrahydrofolate cyclo-ligase985909 986610 1 702 TM.orf0895 ymdB metallophosphoesterase 986721 9870592 339 TM.orf0896 ymdB conserved hypothetical protein 987053 987598 3 546TM.orf0897 conserved hypothetical protein 987733 988482 1 750 TM.orf0898ruvC crossover junction endodeoxyribonuclease 988521 989021 2 501TM.orf0899 ruvA Holliday junction ATP-dependent DNA 989054 989701 3 648helicase TM.orf0900 ruvB Holliday junction DNA helicase B 989698 9907591 1062 TM.orf0901 tol-pal system-associated acyl-CoA 990862 991296 1 435thioesterase TM.orf0902 gatA Glutamyl-tRNA(Gln) amidotransferase 992736991339 −1 1398 subunit A TM.orf0903 tolQ biopolymer transport protein993294 994028 2 735 TM.orf0904 tolR biopolymer transport protein 994032994490 2 459 TM.orf0905 hypothetical protein 995062 994682 −3 381TM.orf0906 TolA protein 995410 995754 1 345 TM.orf0907 tolBtranslocation protein TolB 995751 997130 2 1380 TM.orf0908 Outermembrane protein and related 997284 997796 2 513peptidoglycan-associated lipo protein TM.orf0909 tol-pal system proteinYbgF 998013 999008 2 996 TM.orf0910 tilS putative PP-loop superfamilyATPase 999013 1000494 1 1482 TM.orf0911 ftsH cell division protease1000491 1002434 2 1944 TM.orf0912 folP dihydropteroate synthase 10024611003666 3 1206 TM.orf0913 glmM phosphoglucosamine mutase 1003826 10051813 1356 TM.orf0914 thiD phosphomethylpyrimidine kinase 1005334 1006173 1840 TM.orf0915 phosphomethylpyrimidine kinase 1006170 1007015 2 846TM.orf0916 isomerase 1007384 1008172 3 789 TM.orf0917 GGDEFdomain-containing protein 1009326 1008169 −1 1158 TM.orf0918 serCphosphoserine aminotransferase 1009580 1010746 3 1167 TM.orf0919 serAD-3-phosphoglycerate dehydrogenase 1010864 1012441 3 1578 TM.orf0920hisZ ATP phosphoribosyltransferase regulatory 1012760 1013935 3 1176subunit TM.orf0921 purA adenylosuccinate synthetase 1013949 1015232 21284 TM.orf0922 conserved hypothetical protein 1015398 1017425 2 2028TM.orf0923 conserved hypothetical protein 1017441 1017908 2 468TM.orf0924 IivF branched-chain amino acid ABC transporter, 10186851017936 −2 750 ATP-binding protein TM.orf0925 braF branched-chain aminoacid ABC transporter, 1019443 1018685 −3 759 ATP-binding proteinTM.orf0926 braE inner-membrane translocator 1020607 1019456 −3 1152TM.orf0927 IivH inner-membrane translocator 1021634 1020609 −2 1026TM.orf0928 ABC-type branched-chain amino acid 1023101 1021761 −2 1341transporter TM.orf0929 transcriptional regulator, XRE family protein1023300 1024766 2 1467 TM.orf0930 arlR protein containing responseregulator domain, 1024837 1025208 1 372 but no DNA binding domainTM.orf0931 hypothetical protein 1025230 1025514 1 285 TM.orf0932Na+/solute symporter 1025519 1028239 3 2721 TM.orf0933 transcriptionalregulator MazE 1028523 1028293 −1 231 TM.orf0934 rpoH RNA polymerasefactor sigma-32 1029554 1028631 −2 924 TM.orf0935 rluD Pseudouridylatesynthases, 23S RNA-specific 1031385 1030168 −1 1218 TM.orf0936hypothetical protein 1031422 1031904 1 483 TM.orf0937 Mov34/MPN/PAD-11031901 1032383 2 483 TM.orf0938 (Uracil-5)-methyltransferase 10324631034070 1 1608 TM.orf0939 ltaE putative low specificity L-threoninealdolase 1034067 1035119 2 1053 TM.orf0940 lcfB malonyl-CoA synthase1035551 1037068 3 1518 TM.orf0941 ywbO thiol oxidoreductase FrnE 10371951037962 3 768 TM.orf0942 mdcF malonate transporter 1038916 1037981 −3936 TM.orf0943 N-acetyl-gamma-glutamyl-phosphate 1039420 1040418 1 999reductase TM.orf0944 puuR HTH-type transcriptional regulator puuR1041265 1040717 −3 549 TM.orf0945 omega-amino acid-pyruvateaminotransferase 1041612 1042934 2 1323 TM.orf0946 ald alaninedehydrogenase 1043080 1044204 1 1125 TM.orf0947 addiction moduleantitoxin, RelB/DinJ family 1044340 1044486 1 147 TM.orf0948 putativemultidrug-efflux transporter 1045981 1044515 −3 1467 TM.orf0949 pleCPAS/PAC sensor signal transduction histidine 1046185 1047351 1 1167kinase TM.orf0950 dadA glycine/D-amino acid oxidase 1048626 1047364 −11263 TM.orf0951 ygdD inner membrane protein ygdD 1048788 1049189 2 402TM.orf0952 hypothetical protein 1049217 1049336 2 120 TM.orf0953 AAAATPase central domain protein 1049427 1050308 2 882 TM.orf0954 conservedhypothetical protein 1050366 1051544 2 1179 TM.orf0955 hypotheticalprotein 1051833 1052648 2 816 TM.orf0956 macB ABC transporter relatedprotein 1054578 1052626 −1 1953 TM.orf0957 macA RND family effluxtransporter MFP subunit 1055783 1054575 −2 1209 TM.orf0958 conservedhypothetical protein 1055843 1056796 3 954 TM.orf0959 membrane protein1056813 1058180 2 1368 TM.orf0960 aprE HlyD family secretion protein1059543 1058170 −1 1374 TM.orf0961 aprD ABC transporter, ATP-bindingprotein 1061372 1059540 −2 1833 TM.orf0962 yhjN Putative ammoniamonooxygenase 1062612 1061521 −1 1092 TM.orf0963 histonedeacetylase-like amidohydrolase 1062890 1063828 3 939 TM.orf0964 xseBExonuclease VII small subunit 1063936 1064193 1 258 TM.orf0965 ispAgeranyltranstransferase 1064250 1065176 2 927 TM.orf0966 dxsdeoxyxylulose-5-phosphate synthase 1065466 1067403 1 1938 TM.orf0967yqxC Predicted rRNA methylase 1067420 1068229 3 810 TM.orf0968 fnrtranscriptional regulator, Crp 1068283 1068984 1 702 TM.orf0969 phbC PHBde-polymerase 1070115 1068994 −1 1122 TM.orf0970 conserved hypotheticalprotein 1070686 1070255 −3 432 TM.orf0971 rhodanese-relatedsulfurtransferase 1070835 1071209 2 375 TM.orf0972 hypothetical protein1071363 1071199 −1 165 TM.orf0973 lcfB putative long-chain-fatty-acidCoA ligase 1072995 1071436 −1 1560 TM.orf0974 fadR TetR familytranscriptional regulator 1073737 1073117 −3 621 TM.orf0975 SCP2lipid-transfer protein 1074051 1075232 2 1182 TM.orf0976 conservedhypothetical protein 1075263 1075730 2 468 TM.orf0977 FOX2 MaoC-likedehydratase 1075727 1076113 3 387 TM.orf0978 short-chaindehydrogenase/reductase SDR 1076176 1077003 1 828 TM.orf0979 acyl-CoAdehydrogenase domain protein 1077080 1078240 3 1161 TM.orf0980 conservedhypothetical protein 1078598 1078299 −2 300 TM.orf0981 putativeepimerase PhzC/PhzF-like protein 1079828 1078869 −2 960 TM.orf0982 ynjAconserved hypothetical protein 1080524 1079934 −2 591 TM.orf0983 aroCchorismate synthase 1081676 1080603 −2 1074 TM.orf0984 short-chaindehydrogenase/reductase SDR 1082535 1081723 −1 813 TM.orf0985 yfkH YihYfamily protein 1083690 1082737 −1 954 TM.orf0986 curved DNA-bindingprotein 1084583 1083687 −2 897 TM.orf0987 omp 17 kDa surface antigenprecursor 1085187 1084711 −1 477 TM.orf0988 pdxH pyridoxamine5′-phosphate oxidase 1085540 1086133 3 594 TM.orf0989 fieF cation effluxprotein 1086130 1087056 1 927 TM.orf0990 apt adeninephosphoribosyltransferase 1087198 1087722 1 525 TM.orf0991 tagDNA-3-methyladenine glycosylase I 1088343 1087750 −1 594 TM.orf0992Predicted aminomethyltransferase related to 1089265 1088336 −3 930 GcvTTM.orf0993 glycosyl transferase family protein 1089430 1090422 1 993TM.orf0994 ytcC glycosyl transferase group 1 1090419 1091657 2 1239TM.orf0995 lpsD Glycosyltransferase 1091647 1092765 1 1119 TM.orf0996asnB asparagine synthase 1092798 1094612 2 1815 TM.orf0997 ypfGconserved hypothetical protein 1095734 1094628 −2 1107 TM.orf0998 pleCsensory transduction histidine kinase 1095958 1098360 1 2403 TM.orf0999dcd deoxycytidine triphosphate deaminase 1099261 1099815 1 555TM.orf1000 murA UDP-N-acetylglucosamine enolpyruvyl 1099904 1101193 31290 transferase TM.orf1001 hisG ATP phosphoribosyltransferase catalytic1101245 1101928 3 684 subunit TM.orf1002 hisD histidinol dehydrogenase1101912 1103213 2 1302 TM.orf1003 conserved hypothetical protein 11033271103848 3 522 TM.orf1004 arsC low molecular weight phosphotyrosineprotein 1103908 1104330 1 423 phosphatase TM.orf1005 infA translationinitiation factor 1 1104528 1104746 2 219 TM.orf1006 maf protein 11048661105480 3 615 TM.orf1007 rng ribonuclease G 1105477 1107108 1 1632TM.orf1008 zinc-binding protein 1107142 1107381 1 240 TM.orf1009hypothetical protein 1107832 1108107 1 276 TM.orf1010 hypotheticalprotein 1108278 1108138 −1 141 TM.orf1011 amidohydrolase family protein1108294 1108461 1 168 TM.orf1012 conserved hypothetical protein 11087291109280 1 552 TM.orf1013 metG Methionyl-tRNA synthetase 1110472 1109360−3 1113 TM.orf1014 conserved hypothetical protein 1110999 1111595 2 597TM.orf1015 metQ lipoprotein, YaeC family 1112465 1111677 −2 789TM.orf1016 cysD O-acetylhomoserine/O-acetylserine 1114130 1112832 −21299 sulfhydrylase TM.orf1017 leuA pyruvate carboxyltransferase 11155741114237 −1 1338 TM.orf1018 oxyR transcriptional regulator, LysR family1115785 1116711 1 927 TM.orf1019 nuoE NADH dehydrogenase (ubiquinone) 24kDa 1116819 1117316 2 498 subunit TM.orf1020 putative NAD-dependentformate 1117313 1118869 3 1557 dehydrogenase beta subunit proteinTM.orf1021 yjgC formate dehydrogenase, alpha subunit 1118881 1121721 12841 TM.orf1022 fdhD formate dehydrogenase accessory protein 11217381122535 3 798 TM.orf1023 putative NAD-dependent formate 1122552 11227642 213 dehydrogenase TM.orf1024 nylB 6-aminohexanoate-dimer hydrolase1123952 1122780 −2 1173 TM.orf1025 gsiA ABC transporter ATP-bindingprotein 1125784 1123964 −3 1821 TM.orf1026 puuC aldehyde dehydrogenase(acceptor) 1127313 1125781 −1 1533 TM.orf1027 soxB Sarcosine oxidasesubunit beta 1128720 1127365 −1 1356 TM.orf1028 acetyltransferase1129144 1128707 −3 438 TM.orf1029 FAD dependent oxidoreductase 11294181130563 3 1146 TM.orf1030 appA ABC superfamily ATP binding cassette1130651 1132309 3 1659 transporter substrate-binding protein TM.orf1031appB oligopeptide ABC superfamily ATP binding 1132328 1133278 3 951cassette transporter, membrane protein TM.orf1032 appC ABC transporterpermease 1133284 1134153 1 870 TM.orf1033 gcvA transcriptionalregulatory protein 1134178 1135080 1 903 TM.orf1034 major facilitatorsuperfamily MFS_1 1136380 1135142 −3 1239 TM.orf1035 ywlCSua5/YciO/YrdC/YwlC family protein 1137113 1136391 −2 723 TM.orf1036hypothetical protein 1137943 1137362 −3 582 TM.orf1037 hypotheticalprotein 1138346 1138122 −2 225 TM.orf1038 conserved hypothetical protein1138468 1138971 1 504 TM.orf1039 hypothetical protein 1138968 1139462 2495 TM.orf1040 ycfQ transcriptional regulator, TetR family protein1139539 1140165 1 627 TM.orf1041 fecI RNA polymerase sigma-70 familyprotein 1140504 1141028 2 525 TM.orf1042 putative FecR 1141025 1141987 3963 TM.orf1043 norA oxidoreductase 1143149 1142049 −2 1101 TM.orf1044ycaN transcriptional regulator LysR family 1143262 1144149 1 888TM.orf1045 foxA TonB-dependent siderophore receptor 1144309 1146759 12451 TM.orf1046 PepSY-associated TM helix domain-containing 11467701147993 3 1224 protein TM.orf1047 ntaB Flavin reductase like domainprotein 1148069 1148584 3 516 TM.orf1048 iron-sulfur cluster repairdi-iron protein 1148678 1149136 3 459 TM.orf1049 signal peptide protein1149672 1149154 −1 519 TM.orf1050 phosphoglycerate mutase family protein1149860 1150432 3 573 TM.orf1051 ybfB major facilitator superfamilyMFS_1 1150696 1151955 1 1260 TM.orf1052 hypothetical protein 11519791152560 2 582 TM.orf1053 ubiG 3-demethylubiquinone-9 3-O- 11534401152544 −1 897 methyltransferase TM.orf1054 peptidase M50 11545971153437 −2 1161 TM.orf1055 Lantibiotic dehydratase domain protein1157020 1154594 −3 2427 TM.orf1056 hypothetical protein 1157871 1157017−1 855 TM.orf1057 conserved hypothetical protein 1158788 1157871 −2 918TM.orf1058 eamB Lysine exporter protein (LYSE/YGGA) 1159483 1158854 −3630 TM.orf1059 radical SAM domain-containing protein 1161690 1159567 −12124 TM.orf1060 hypothetical protein 1162116 1161901 −1 216 TM.orf1061conserved hypothetical protein 1162425 1162712 2 288 TM.orf1062 yfkNalkaline phosphatase 1162922 1167649 3 4728 TM.orf1063 prtC Secretedprotease C 1167664 1169685 1 2022 TM.orf1064 TolC family type Isecretion outer membrane 1169792 1171222 3 1431 protein TM.orf1065 paxBputative exotoxin translocation ATP-binding 1171219 1173030 1 1812protein PaxB TM.orf1066 cyaD type I secretion membrane fusion protein,1173020 1174321 3 1302 HlyD family TM.orf1067 conserved hypotheticalprotein 1175412 1174330 −1 1083 TM.orf1068 pleC Signal transductionhistidine kinase 1175791 1177161 1 1371 TM.orf1069 acoD acetaldehydedehydrogenase 1177358 1178905 3 1548 TM.orf1070 conserved hypotheticalprotein 1178941 1179459 1 519 TM.orf1071 conserved hypothetical protein1179570 1180430 2 861 TM.orf1072 pyridoxamine 5′-phosphateoxidase-related 1180529 1180996 3 468 FMN-binding protein TM.orf1073conserved hypothetical protein 1181468 1180980 −2 489 TM.orf1074 fliSflagellar protein FliS 1182138 1181662 −1 477 TM.orf1075 fliD flagellarhook-associated protein 2 (FliD, 1184293 1182170 −3 2124 filament capprotein) precursor TM.orf1076 flagellar protein FlaG 1184798 1184376 −2423 TM.orf1077 flagellin 1186200 1185037 −1 1164 TM.orf1078 flagellindomain protein 1187778 1186615 −1 1164 TM.orf1079 alkylhydroperoxidaselike protein, AhpD family 1188104 1188586 3 483 TM.orf1080 conservedhypothetical protein 1189248 1188577 −1 672 TM.orf1081 conservedhypothetical protein 1191698 1189254 −2 2445 TM.orf1082 celY cellulase1192993 1191689 −3 1305 TM.orf1083 cellulose synthase subunit B 11957791192990 −1 2790 TM.orf1084 bcsA putative cellulose synthase catalyticsubunit 1198133 1195797 −2 2337 TM.orf1085 fliB Lysine-N-methylase1199528 1198353 −2 1176 TM.orf1086 fliW Flagellar assembly factor fliW1200072 1199554 −1 519 TM.orf1087 flagellar hook-associated protein FlgL1201094 1200192 −2 903 TM.orf1088 flgK flagellar hook-associated protein1202984 1201173 −2 1812 TM.orf1089 hypothetical protein 1203537 1203016−1 522 TM.orf1090 chemotactic signal-response protein CheL 12039521203569 −3 384 TM.orf1091 flgI flagellar P-ring protein precursor1205127 1203964 −1 1164 TM.orf1092 flagellar assembly regulator FliX1205467 1205895 1 429 TM.orf1093 dksA DnaK suppressor protein 12060171206433 3 417 TM.orf1094 pleC putative sensory box histidine kinase1206708 1209452 2 2745 TM.orf1095 flagellar basal body L-ring protein1210260 1209466 −1 795 TM.orf1096 flgA flagellar basal body P-ringbiosynthesis protein 1211316 1210324 −1 993 FlgA TM.orf1097 flgGflagellar basal-body rod protein FlgG 1212149 1211364 −2 786 TM.orf1098flgF flagellar basal-body rod protein 1212950 1212198 −2 753 TM.orf1099fliL flagellar basal body-associated protein 1213326 1213868 2 543TM.orf1100 fliM flagellar motor switch protein FliM 1213924 1215030 11107 TM.orf1101 ATPase involved in DNA repair 1215027 1215620 2 594TM.orf1102 FlaA locus 229 kDa protein 1215704 1216519 3 816 TM.orf1103cheY Response regulator receiver 1216543 1216929 1 387 TM.orf1104chemotaxis protein CheZ 1216979 1217641 3 663 TM.orf1105 PPE-repeatprotein (cell mobility) 1217692 1218354 1 663 TM.orf1106 ytxE chemotaxisMotB protein 1218351 1219100 2 750 TM.orf1107 conserved hypotheticalprotein 1219097 1222261 3 3165 TM.orf1108 rlmI methyltransferase 12224021223379 1 978 TM.orf1109 ysgA rRNA methylase 1223383 1224210 1 828TM.orf1110 hypothetical protein 1224235 1224495 1 261 TM.orf1111 dhaRTetR/AcrR family transcriptional regulator 1224589 1225215 1 627TM.orf1112 hypothetical protein 1224617 1224492 −2 126 TM.orf1113 linC2,5-dichloro-2,5-cyclohexadiene-1,4- 1225281 1226045 2 765dioldehydrogenase TM.orf1114 DMT family permease 1226948 1226052 −2 897TM.orf1115 lrhA LysR family transcriptional regulator 1227106 1227975 1870 TM.orf1116 fliP flagellar biosynthetic protein FliP 1229494 1228724−3 771 TM.orf1117 hypothetical protein 1229949 1229491 −1 459 TM.orf1118hypothetical protein 1230402 1230043 −1 360 TM.orf1119 ylqH flagellarbiosynthetic protein FlhB 1230732 1230448 −1 285 TM.orf1120 csrA carbonstorage regulator 1231119 1230856 −1 264 TM.orf1121 flgB flagellar basalbody rod protein FlgB 1231481 1231903 3 423 TM.orf1122 flgC flagellarbasal-body rod protein FlgC 1231952 1232359 3 408 TM.orf1123 fliEflagellar hook-basal body complex protein FliE 1232420 1232734 3 315TM.orf1124 yscS flagellar biosynthetic protein FNQ 1232807 1233082 3 276TM.orf1125 fliR flagellar biosynthesis protein FliR 1233102 1233857 2756 TM.orf1126 flhB flagellar biosynthetic protein FlhB 1233875 12349543 1080 TM.orf1127 cell cycle histidine kinase CckA 1235020 1236945 11926 TM.orf1128 hypothetical protein 1237585 1237100 −3 486 TM.orf1129recA RecA protein 1237929 1239029 2 1101 TM.orf1130 alaS alanyl-tRNAsynthetase 1239345 1241999 2 2655 TM.orf1131 conserved hypotheticalprotein 1243214 1242069 −2 1146 TM.orf1132 rhsD nematicidal protein 21248254 1243263 −2 4992 TM.orf1133 putative acetyltransferase 12490031248464 −3 540 TM.orf1134 yjiA cobalamin synthesis protein CobW 12501031249000 −1 1104 TM.orf1135 3-hydroxyacyl-CoA dehydrogenase (hdb-1)1250300 1251193 3 894 TM.orf1136 icd isocitrate dehydrogenase 12525431251296 −3 1248 TM.orf1137 hypothetical protein 1252775 1253668 3 894TM.orf1138 ygaU protein containing LysM domain 1253779 1255026 1 1248TM.orf1139 ywfA MFS-type transporter ywfA 1256268 1255027 −1 1242TM.orf1140 hypothetical protein 1259653 1259456 −3 198 TM.orf1141hypothetical protein 1261070 1259721 −2 1350 TM.orf1142 yezE TetR familytranscriptional regulator 1261874 1261266 −2 609 TM.orf1143 conservedhypothetical protein 1261993 1262910 1 918 TM.orf1144 yfcG glutathioneS-transferase domain-containing 1262966 1263670 3 705 protein TM.orf1145yfcG Glutathione S-transferase domain protein 1263679 1264389 1 711TM.orf1146 rbsC methyl-galactoside transport system permease 12654691264411 −1 1059 protein TM.orf1147 ABC transporter ATP-binding protein1266995 1265466 −2 1530 TM.orf1148 torT ABC transportersubstrate-binding protein 1268159 1267098 −2 1062 TM.orf1149 kipA ureaamidolyase related protein 1269275 1268304 −2 972 TM.orf1150 allophanatehydrolase subunit 1 1270140 1269265 −1 876 TM.orf1151 accC biotincarboxylase 1271514 1270150 −1 1365 TM.orf1152 acetyl-CoA carboxylasebiotin carboxyl carrier 1271758 1271522 −3 237 protein TM.orf1153conserved hypothetical protein 1272647 1271865 −2 783 TM.orf1154 cynRtranscriptional regulator 1272913 1273836 1 924 TM.orf1155 cupinsuperfamily protein 1274097 1275260 2 1164 TM.orf1156 pfpI proteinase1275836 1275285 −2 552 TM.orf1157 conserved hypothetical protein 12765161275911 −3 606 TM.orf1158 ynjF phosphatidylglycerophosphate synthase1276707 1277315 2 609 TM.orf1159 conserved hypothetical protein 12774541278356 2 903 TM.orf1160 ydjF transcriptional regulator, DeoR family1278360 1279133 2 774 TM.orf1161 ytfG oxidoreductase ytfG 12800161279138 −1 879 TM.orf1162 ytcD putative HTH-type transcriptionalregulator 1280138 1280497 3 360 TM.orf1163 mdtC AcrB 1283531 1280535 −22997 TM.orf1164 Membrane-fusion protein 1284902 1283676 −2 1227TM.orf1165 padR transcriptional regulator, PadR family protein 12854561284899 −3 558 TM.orf1166 dtdp-glucose 4,6-dehydratase 1286620 1285598−3 1023 TM.orf1167 NAD-dependent epimerase/dehydratase 1287306 1286620−1 687 TM.orf1168 methyltransferase, UbiE 1288319 1287462 −2 858TM.orf1169 glmU molybdopterin binding domain-containing 1290137 1288446−2 1692 protein TM.orf1170 pucA xanthine dehydrogenase accessory factor1290925 1290194 −3 732 TM.orf1171 yqeB XdhC/CoxI family protein 12912801290942 −2 339 TM.orf1172 VWA containing CoxE family protein 12925451291286 −3 1260 TM.orf1173 ATPase associated with various cellular1293426 1292542 −1 885 activities TM.orf1174 cutM carbon-monoxidedehydrogenase (acceptor) 1294473 1293673 −1 801 TM.orf1175 cutL carbonmonoxide dehydrogenase large chain 1296922 1294535 −3 2388 TM.orf1176cutS carbon monoxide dehydrogenase small chain 1297463 1296993 −2 471TM.orf1177 ghrA D-isomer specific 2-hydroxyacid 1298852 1297899 −2 954dehydrogenase, NAD-binding TM.orf1178 hypothetical protein 12991901298951 −3 240 TM.orf1179 hypothetical protein 1299598 1299329 −3 270TM.orf1180 narP two-component response regulator 1299787 1300494 1 708TM.orf1181 appC ABC transporter permease protein 1301407 1300499 −3 909TM.orf1182 gsiC binding-protein-dependent transport systems 13023421301404 −1 939 inner membrane component TM.orf1183oligopeptide/dipeptide ABC transporter, 1303335 1302349 −1 987 ATPasesubunit TM.orf1184 ABC transporter ATP binding protein 1304318 1303389−2 930 TM.orf1185 dppA oligopeptide ABC transpoter oligopeptide- 13058891304315 −3 1575 binding protein TM.orf1186 conserved hypotheticalprotein 1306808 1305963 −2 846 TM.orf1187 TetR family transcriptionalregulator 1306998 1307657 2 660 TM.orf1188 conserved hypotheticalprotein 1307758 1308891 1 1134 TM.orf1189 allophanate hydrolase 13092731308911 −3 363 TM.orf1190 rutB isochorismatase family protein 13099501309270 −1 681 TM.orf1191 yufO putative ABC transporter ATP-bindingprotein 1311546 1309969 −1 1578 TM.orf1192 isochorismatase familyprotein 1312235 1311543 −2 693 TM.orf1193 yufQ ABC transporter permeaseprotein 1313199 1312273 −1 927 TM.orf1194 putative simple sugartransport system 1314322 1313204 −3 1119 permease protein TM.orf1195 medsimple sugar transport system substrate- 1315523 1314414 −2 1110 bindingprotein TM.orf1196 ydfH transcriptional regulator, GntR family 13159161316635 3 720 TM.orf1197 Urea amidolyase 1316648 1318471 3 1824TM.orf1198 anti-sigma-factor antagonist 1318884 1318495 −1 390TM.orf1199 mobA molybdopterin-guanine dinucleotide 1319614 1318931 −3684 biosynthesis protein A TM.orf1200 sugE small multidrug resistanceprotein 1319848 1320168 1 321 TM.orf1201 cold-shock DNA-bindingdomain-containing 1320334 1320687 1 354 protein TM.orf1202 yiaO TRAPdicarboxylate transporter- DctP subunit 1320940 1322001 1 1062TM.orf1203 hypothetical protein 1322006 1322500 3 495 TM.orf1204 TRAPdicarboxylate transporter, DctM subunit 1322497 1323801 1 1305TM.orf1205 long-chain-fatty-acid--CoA ligase 1323806 1324267 3 462TM.orf1206 lcfB AMP-dependent synthetase and ligase 1324240 1325400 11161 TM.orf1207 hypothetical protein 1325530 1325820 1 291 TM.orf1208ygaZ AzlC family protein 1325859 1326626 2 768 TM.orf1209 branched-chainamino acid transport 1326626 1326946 3 321 TM.orf1210 pepN membranealanyl aminopeptidase 1327064 1329727 3 2664 TM.orf12114-chloro-3-hydroxybutyrate hydrolase 1330982 1329804 −2 1179 TM.orf1212transcriptional regulator, AraC family 1331259 1331786 2 528 TM.orf1213thcR transcriptional regulator, AraC family 1331747 1332259 3 513TM.orf1214 Methyl-accepting chemotaxis protein 1333700 1332351 −2 1350TM.orf1215 ycbC conserved hypothetical protein 1333892 1334713 3 822TM.orf1216 cph2 Phytochrome-like protein cph2 1334862 1337087 2 2226TM.orf1217 YbaK/prolyl-tRNA synthetase associated 1337299 1337096 −3 204region TM.orf1218 ppnK Predicted sugar kinase 1338173 1337343 −2 831TM.orf1219 moaA molybdenum cofactor biosynthesis protein A 13392241338181 −1 1044 TM.orf1220 conserved hypothetical protein 13404861339377 −2 1110 TM.orf1221 conserved hypothetical protein 13410121340800 −1 213 TM.orf1222 hypothetical protein 1341287 1341955 3 669TM.orf1223 cheA CheA Signal transduction histidine Kinases 13420751344816 1 2742 (STHK) TM.orf1224 cheW Chemotaxis signal transductionprotein 1344813 1345310 2 498 TM.orf1225 cheY FOG: CheY-like receiver1345407 1345772 2 366 TM.orf1226 cheB chemotaxis-specific methylesterase1345820 1347007 3 1188 TM.orf1227 chemotaxis protein methyltransferaseCheR 1347007 1347816 1 810 TM.orf1228 ctrA two-component responseregulator 1348673 1347942 −2 732 TM.orf1229 fliI flagellum-specific ATPsynthase 1348922 1350259 3 1338 TM.orf1230 hypothetical protein 13502661350706 3 441 TM.orf1231 conserved hypothetical protein 1351202 13523833 1182 TM.orf1232 yfiL ABC transporter related protein 1352388 1353380 2993 TM.orf1233 ABC transporter permease protein 1353373 1354323 1 951TM.orf1234 ybfB major facilitator superfamily MFS_1 1355611 1354358 −31254 TM.orf1235 yjdC transcriptional regulator, TetR family protein1356283 1355675 −3 609 TM.orf1236 conserved hypothetical protein 13568031356336 −2 468 TM.orf1237 conserved hypothetical protein 1357954 1356872−3 1083 TM.orf1238 ylxH FleN 1361632 1360847 −3 786 TM.orf1239 FlhF1362951 1361629 −1 1323 TM.orf1240 flhA flagellar biosynthesis pathway,component 1365160 1363037 −3 2124 FlhA TM.orf1241 flbD Responseregulator containing CheY-like 1366774 1365245 −3 1530 receiver,AAA-type ATPase, and DNA-binding domains TM.orf1242 pomA chemotaxisprotein 1367656 1366889 −3 768 TM.orf1243 fliN flagellar motor switchprotein 1368111 1367758 −1 354 TM.orf1244 flagellar assembly protein H1368888 1368181 −1 708 TM.orf1245 fliG flagellar motor switch protein G1369955 1368933 −2 1023 TM.orf1246 fliF flagellar MS-ring protein1371668 1369962 −2 1707 TM.orf1247 conserved hypothetical protein1372410 1372048 −1 363 TM.orf1248 flgE Flagellar hook protein flgE1374276 1372573 −1 1704 TM.orf1249 flgD flagellar basal-body rodmodification protein 1375223 1374444 −2 780 FlgD TM.orf1250 flagellarhook-length control protein 1378290 1375246 −1 3045 TM.orf1251 pleCPAS/PAC sensor signal transduction histidine 1379682 1378405 −1 1278kinase TM.orf1252 chaC ChaC-like protein 1380628 1379810 −3 819TM.orf1253 acetoacetyl-CoA synthetase 1380890 1382917 3 2028 TM.orf1254cph2 GGDEF family protein 1383065 1384003 3 939 TM.orf1255 soxR LysRfamily transcriptional regulator 1384044 1384946 2 903 TM.orf1256 ccpAcytochrome c551 peroxidase precursor 1385079 1386113 2 1035 TM.orf1257conserved hypothetical protein 1387205 1386129 −2 1077 TM.orf1258anthranilate synthase 1389504 1387342 −1 2163 TM.orf1259 bigR ArsRfamily transcriptional regulator 1389867 1390208 2 342 TM.orf1260YeeE/YedE family protein 1390205 1390642 3 438 TM.orf1261 membraneprotein 1390709 1391170 3 462 TM.orf1262 metallo-beta-lactamase familyprotein 1391175 1392083 2 909 TM.orf1263 conserved hypothetical protein1392135 1392353 2 219 TM.orf1264 sulfate transporter 1392393 1394147 21755 TM.orf1265 conserved hypothetical protein 1394285 1395940 3 1656TM.orf1266 conserved hypothetical protein 1396591 1395983 −3 609TM.orf1267 conserved hypothetical protein 1397805 1396579 −1 1227TM.orf1268 ydeM Anaerobic sulfatase-maturating enzyme 1399284 1397824 −11461 homolog ydeM TM.orf1269 hypothetical protein 1399613 1399281 −2 333TM.orf1270 bdlA methyl-accepting chemotaxis protein 1401211 1399727 −31485 TM.orf1271 conserved hypothetical protein 1401687 1402514 2 828TM.orf1272 dctP TRAP dicarboxylate transporter- DctP subunit 14025991403675 2 1077 TM.orf1273 conserved hypothetical protein 1403734 14043421 609 TM.orf1274 siaT TRAP dicarboxylate transporter, DctM subunit1404412 1405698 1 1287 TM.orf1275 conserved hypothetical protein 14057881406468 2 681 TM.orf1276 gatA Glutamyl-tRNA(Gln) amidotransferasesubunit A 1406534 1407892 3 1359 TM.orf1277 amidase 1407916 1408734 1819 TM.orf1278 namA NADH: flavin oxidoreductase/NADH oxidase 14088591410016 3 1158 TM.orf1279 hypothetical protein 1410145 1410306 1 162TM.orf1280 mntB ABC transporter component 1410303 1411064 2 762TM.orf1281 mntC ABC transporter permease protein 1411083 1411967 2 885TM.orf1282 periplasmic solute binding protein 1411964 1412929 3 966TM.orf1283 helix-turn-helix type 11 domain-containing 1413590 1412898 −2693 protein TM.orf1284 conserved hypothetical protein 1413958 1413596 −3363 TM.orf1285 Peci enoyl-CoA hydratase/isomerase 1415250 1414009 −11242 TM.orf1286 lcfB AMP-binding domain protein 1417269 1415335 −1 1935TM.orf1287 Short-chain alcohol dehydrogenase of 1417502 1418254 3 753unknown specificity TM.orf1288 rutR transcriptional regulator, TetRfamily protein 1418422 1419123 1 702 TM.orf1289 alsB D-allose-bindingperiplasmic protein 1419446 1420483 3 1038 TM.orf1290 rbsA ABCtransporter related protein 1420601 1422166 3 1566 TM.orf1291 rbsCinner-membrane translocator 1422163 1423200 1 1038 TM.orf1292 sorCtranscriptional regulator, DeoR family 1423261 1424268 1 1008 TM.orf1293glpD Glycerol-3-phosphate dehydrogenase 1424428 1426194 1 1767TM.orf1294 aldolase protein 1426249 1427091 1 843 TM.orf1295 lyxL-xylulose kinase protein 1427157 1428653 2 1497 TM.orf1296 putativedehydrogenase 1429452 1428658 −1 795 TM.orf1297 conserved hypotheticalprotein 1430636 1429497 −2 1140 TM.orf1298 TetR family transcriptionalregulator 1430704 1431333 1 630 TM.orf1299 conserved hypotheticalprotein 1433151 1431346 −1 1806 TM.orf1300 fecI ECF subfamily RNApolymerase sigma factor 1433359 1433928 1 570 TM.orf1301 sigma factorregulatory protein FecR/PupR 1433928 1434968 2 1041 family TM.orf1302irgA TonB-dependent receptor 1435150 1437471 1 2322 TM.orf1303hypothetical protein 1437570 1437866 2 297 TM.orf1304 conservedhypothetical protein 1437863 1439428 3 1566 TM.orf1305 hypotheticalprotein 1439425 1439766 1 342 TM.orf1306 ywfM transporter ywfM 14406491439717 −3 933 TM.orf1307 TetR family transcriptional regulator 14413441440688 −1 657 TM.orf1308 hypothetical protein 1442035 1442223 1 189TM.orf1309 conserved hypothetical protein 1442292 1442507 2 216TM.orf1310 hypothetical protein 1442531 1442734 3 204 TM.orf1311 pqqECoenzyme PQQ synthesis protein E 1442877 1444391 2 1515 TM.orf1312TonB-dependent receptor 1444961 1447168 3 2208 TM.orf1313rhizobiocin/RTX toxin and hemolysin-type 1448826 1447252 −1 1575 calciumbinding protein TM.orf1314 cya calcium binding hemolysin protein 14525881449004 −1 3585 TM.orf1315 hemolysin-type calcium-binding repeat family1454350 1452965 −3 1386 protein TM.orf1316 cya proprotein convertase P1457577 1454641 −1 2937 TM.orf1317 araQ ABC superfamily ATP bindingcassette 1458683 1457808 −2 876 transporter, membrane protein TM.orf1318yurN inner membrane component of binding- 1459558 1458680 −3 879protein-dependent transport system TM.orf1319 ugpB extracellularsolute-binding protein 1460942 1459650 −2 1293 TM.orf1320 ugpC ABCsuperfamily ATP binding cassette 1462072 1460972 −3 1101 transporter,ABC protein TM.orf1321 glpR transcriptional regulator, DeoR family1462836 1462069 −1 768 TM.orf1322 ypeA acetyltransferase, GNAT family1463231 1463707 3 477 TM.orf1323 aprE peptidase S8 and S53, subtilisin,kexin, 1463916 1466324 2 2409 sedolisin TM.orf1324 WD-40 repeat protein1467482 1466412 −2 1071 TM.orf1325 cobalamin synthesis protein, P47K1468521 1467466 −1 1056 TM.orf1326 conserved hypothetical protein1468785 1469552 2 768 TM.orf1327 hypothetical protein 1469561 1469698 3138 TM.orf1328 lysine exporter protein LysE/YggA 1470401 1469760 −2 642TM.orf1329 AsnC family transcriptional regulator 1470889 1470398 −3 492TM.orf1330 hxuC TonB-dependent hemoglobin 1471277 1473346 3 2070TM.orf1331 hemP hemin uptake protein HemP 1473394 1473591 1 198TM.orf1332 hmuS hemin transport protein 1473602 1474663 3 1062TM.orf1333 hmuT periplasmic binding protein 1474663 1475559 1 897TM.orf1334 hmuU putative ABC-type Fe3+-siderophore transport 14755641476712 3 1149 system, permease component TM.orf1335 hmuV ABC-type hemintransport system, ATPase 1476730 1477518 1 789 component TM.orf1336 bioIcytochrome P450 hydroxylase 1478788 1477532 −3 1257 TM.orf1337 mscLlarge conductance mechanosensitive channel 1479044 1479484 3 441 proteinTM.orf1338 conserved hypothetical protein 1481042 1479507 −2 1536TM.orf1339 outer membrane protein 1481600 1482310 3 711 TM.orf1340conserved hypothetical protein 1482576 1483103 2 528 TM.orf1341 tolQMotA/TolQ/ExbB proton channel 1483635 1484510 2 876 TM.orf1342hypothetical protein 1484536 1484949 1 414 TM.orf1343 hypotheticalprotein 1484946 1485374 2 429 TM.orf1344 tonB TonB-like protein 14853711486483 3 1113 TM.orf1345 glycosyl transferase family 2 1489295 1486617−2 2679 TM.orf1346 hypothetical protein 1489715 1489996 3 282 TM.orf1347triacylglycerol lipase 1491806 1490001 −2 1806 TM.orf1348 glkglucokinase 1492005 1493099 2 1095 TM.orf1349 agmatine deiminase 14931441494274 3 1131 TM.orf1350 pirin 1495175 1494279 −2 897 TM.orf1351 ykvYpeptidase M24 1496516 1495320 −2 1197 TM.orf1352 major facilitatortransporter 1497829 1496525 −3 1305 TM.orf1353 pchR AraC familytranscriptional regulator 1498011 1499015 2 1005 TM.orf1354 cationefflux protein 1499913 1499005 −1 909 TM.orf1355 BGL2 glycosidehydrolase, family 17 1501794 1500118 −1 1677 TM.orf1356 cobC putativethreonine-phosphate decarboxylase 1502952 1501879 −1 1074 TM.orf1357cobQ adenosylcobyric acid synthase 1504421 1502949 −2 1473 TM.orf1358btuB TonB-dependent receptor 1506335 1504440 −2 1896 TM.orf1359PRC-barrel domain-containing protein 1507420 1506827 −3 594 TM.orf1360conserved hypothetical protein 1507743 1508477 2 735 TM.orf1361 hrpBHrpA-like helicase 1508518 1511061 1 2544 TM.orf1362 rhodanese-relatedsulfurtransferase 1511485 1511084 −3 402 TM.orf1363 gcvA LysR familytranscriptional regulator 1511592 1512470 2 879 TM.orf1364glutamyl-tRNA(Gln) amidotransferase subunit A 1512585 1513982 2 1398TM.orf1365 insulin-cleaving metalloproteinase outer 1514113 1515390 11278 membrane protein TM.orf1366 conserved hypothetical protein 15154821517041 3 1560 TM.orf1367 conserved hypothetical protein 1517041 15181171 1077 TM.orf1368 twin-arginine translocation pathway signal 15181331519266 1 1134 TM.orf1369 ycnE Putative monooxygenase ycnE 15196331519343 −3 291 TM.orf1370 Haloacid dehalogenase domain protein 15204331519759 −1 675 hydrolase TM.orf1371 mmsB 3-hydroxyisobutyratedehydrogenase 1521512 1520613 −2 900 TM.orf1372 acyl-CoA dehydrogenasedomain-containing 1522771 1521620 −3 1152 protein TM.orf1373 mmsAmethylmalonate-semialdehyde dehydrogenase 1524365 1522869 −2 1497TM.orf1374 mauR transcriptional regulator 1524594 1525505 2 912TM.orf1375 NupC family protein 1525694 1526542 3 849 TM.orf1376 yutK Na+dependent nucleoside transporter 1526520 1526921 2 402 TM.orf1377radical SAM/B12 binding domain protein 1527265 1529292 1 2028 TM.orf1378aminoglycoside phosphotransferase 1529454 1530494 2 1041 TM.orf1379 gnoshort-chain dehydrogenase/reductase SDR 1530546 1531322 2 777 TM.orf1380Mecr nuclear receptor binding factor 1 1531420 1532424 1 1005 TM.orf1381yhfT fatty-acyl-CoA synthase 1532466 1534115 2 1650 TM.orf1382 TetRfamily transcriptional regulator 1534242 1534850 2 609 TM.orf1383 mdtARND family efflux transporter MFP subunit 1534942 1536090 1 1149TM.orf1384 nolG acriflavin resistance protein 1536146 1539379 3 3234TM.orf1385 znuA periplasmic solute binding protein 1540543 1539479 −31065 TM.orf1386 zur ferric uptake regulator, Fur family 1540644 15412012 558 TM.orf1387 znuC high-affinity zinc uptake system ATP-binding1541198 1542064 3 867 protein ZnuC TM.orf1388 znuB permease of ABC zinctransporter ZnuB 1542083 1542883 3 801 TM.orf1389 conserved hypotheticalprotein 1543006 1544046 1 1041 TM.orf1390 potI binding-protein-dependenttransport systems 1544920 1544096 −3 825 inner membrane componentTM.orf1391 potB binding-protein-dependent transport systems 15457921544917 −1 876 inner membrane component TM.orf1392 potAspermidine/putrescine transport system ATP- 1546891 1545794 −3 1098binding protein TM.orf1393 twin-arginine translocation pathway signal1548148 1546973 −3 1176 TM.orf1394 UspA domain-containing protein1548687 1549520 2 834 TM.orf1395 major facilitator family protein1550818 1549541 −3 1278 TM.orf1396 phosphatase 1551026 1551763 3 738TM.orf1397 hypothetical protein 1552567 1551773 −3 795 TM.orf1398lipoprotein, putative 1553277 1552606 −1 672 TM.orf1399 ABC transportsystem substrate-binding 1554268 1553270 −3 999 protein TM.orf1400 ABCtransporter related protein 1555138 1554287 −3 852 TM.orf1401 ABCtransport system permease protein 1556336 1555143 −2 1194 TM.orf1402lcfB malonyl-CoA synthase 1557997 1556462 −3 1536 TM.orf1403 crtcarnitinyl-CoA dehydratase 1558856 1558041 −2 816 TM.orf1404 ydfH GntRfamily transcriptional regulator 1559600 1558914 −2 687 TM.orf1405Tripartite ATP-independent periplasmic 1559821 1560336 1 516 transporterDctQ component TM.orf1406 TRAP dicarboxylate transporter, DctM subunit1560333 1561619 2 1287 TM.orf1407 yiiZ TRAP dicarboxylate transporter-DctP subunit 1561698 1562741 2 1044 TM.orf1408 acyl-CoA synthase 15627251563960 1 1236 TM.orf1409 caiD enoyl-CoA hydratase 1564010 1564804 3 795TM.orf1410 lip2 lipolytic enzyme 1564915 1565889 1 975 TM.orf1411hypothetical protein 1565897 1566424 3 528 TM.orf1412 gatAGlutamyl-tRNA(Gln) amidotransferase subunit A 1566459 1567871 2 1413TM.orf1413 conserved hypothetical protein 1568030 1568968 3 939TM.orf1414 nahD 2-hydroxychromene-2-carboxylate isomerase 15690701569678 1 609 TM.orf1415 dkgB oxidoreductase 1569801 1570634 2 834TM.orf1416 nitroreductase 1571352 1570693 −1 660 TM.orf1417 pleCtwo-component sensor histidine kinase 1571579 1573522 3 1944 TM.orf1418ddpA extracellular solute-binding protein 1573651 1575285 1 1635TM.orf1419 dppB ABC transporter membrane spanning protein 15752821576328 2 1047 (oligopeptide) TM.orf1420 ddpC binding-protein-dependenttransport systems 1576325 1577242 3 918 inner membrane componentTM.orf1421 dppD ABC transporter related protein 1577239 1578087 1 849TM.orf1422 molybdopterin oxidoreductase 1581154 1578839 −3 2316TM.orf1423 conserved hypothetical protein 1582223 1581204 −2 1020TM.orf1424 TRAP transporter, 4TM/12TM fusion protein 1584193 1582220 −31974 TM.orf1425 31 kDa immunogenic protein 1585241 1584273 −2 969TM.orf1426 yagI Transcriptional regulator, IclR family 1586146 1585379−3 768 TM.orf1427 conserved hypothetical protein 1586343 1587263 2 921TM.orf1428 acetone carboxylase, gamma subunit 1587801 1587295 −1 507TM.orf1429 acetone carboxylase alpha subunit 1590220 1587902 −3 2319TM.orf1430 hyuA Acetone carboxylase beta subunit; AcxA 1592448 1590271−1 2178 TM.orf1431 stc Fis family GAF modulated sigma54 specific 15928041594699 3 1896 transcriptional regulator TM.orf1432 conservedhypothetical protein 1595182 1594706 −3 477 TM.orf1433 cation effluxprotein precursor 1595344 1596237 1 894 TM.orf1434 lcfA AMP-dependentsynthetase and ligase 1596385 1598199 1 1815 TM.orf1435 grsTthioesterase domain protein 1599028 1598234 −3 795 TM.orf1436 ppxExopolyphosphatase 1599825 1600829 2 1005 TM.orf1437 rlmE 23S rRNAmethylase 1600879 1601694 1 816 TM.orf1438 guaB inosine-5′-monophosphatedehydrogenase 1601944 1603401 1 1458 TM.orf1439 rsmB tRNA and rRNAcytosine-C5-methylase 1603530 1604882 2 1353 TM.orf1440 hypotheticalprotein 1604974 1605618 1 645 TM.orf1441 yhiD MgtC/SapB transporter1605701 1606150 3 450 TM.orf1442 cpx xanthine/uracil/vitamin C permease1606354 1608045 1 1692 TM.orf1443 ydjP AB hydrolase superfamily proteinydjP 1608128 1608985 3 858 TM.orf1444 yetK transporter yetK 16090881610164 3 1077 TM.orf1445 guaA bifunctional GMP synthase/glutamine1610289 1611836 2 1548 amidotransferase protein TM.orf1446 TetR familytranscriptional regulator 1612122 1612697 2 576 TM.orf1447 3-betahydroxysteroid 1612702 1613676 1 975 dehydrogenase/isomerase TM.orf1448ydjG putative oxidoreductase 1614840 1613758 −1 1083 TM.orf1449 ycaNLysR family transcriptional regulator 1615063 1615968 1 906 TM.orf1450ndvA Beta-(1-->2)glucan export ATP- 1619031 1616164 −1 2868binding/permease protein ndvA TM.orf1451 bacteriocin/lantibiotic ABCtransporter 1621187 1619037 −2 2151 TM.orf1452 membrane-fusion protein1622477 1621188 −2 1290 TM.orf1453 putative secreted protein 16231001622522 −3 579 TM.orf1454 silP cation transport ATPase 1625423 1623330−2 2094 TM.orf1455 conserved hypothetical protein 1626219 1627070 2 852TM.orf1456 PEBP family protein 1627587 1627084 −1 504 TM.orf1457 yeaMAraC family transcriptional regulator 1628424 1627657 −1 768 TM.orf1458conserved hypothetical protein 1628546 1629337 3 792 TM.orf1459conserved hypothetical protein 1629444 1630667 2 1224 TM.orf1460conserved hypothetical protein 1630670 1631500 3 831 TM.orf1461conserved hypothetical protein 1631513 1632250 3 738 TM.orf1462 yuxNTetR family regulatory protein 1632939 1632262 −1 678 TM.orf1463glycosyl transferase family protein 1633072 1634304 1 1233 TM.orf1464putative integral membrane protein 1635052 1634318 −3 735 TM.orf1465hypothetical protein 1635191 1635475 3 285 TM.orf1466 gdhAmembrane-bound PQQ-dependent 1635644 1636300 3 657 dehydrogenase,glucose/quinate/shikimate family TM.orf1467 sodium/hydrogen exchanger1637571 1636267 −1 1305 TM.orf1468 chaperone protein HtpG 16377471638415 3 669 TM.orf1469 molybdopterin oxidoreductase, iron-sulfur1638399 1640249 2 1851 binding subunit TM.orf1470 hmeA molybdopterinoxidoreductase, iron-sulfur 1640246 1641451 3 1206 binding subunitTM.orf1471 Polysulphide reductase NrfD 1641448 1642797 1 1350 TM.orf1472transmembrane prediction 1642790 1643350 3 561 TM.orf1473 conservedhypothetical protein 1643347 1643880 1 534 TM.orf1474 conservedhypothetical protein 1643877 1644626 2 750 TM.orf1475 conservedhypothetical protein 1644598 1644987 1 390 TM.orf1476 hypotheticalprotein 1644984 1645415 2 432 TM.orf1477 electron transport proteinSCO1/SenC 1645412 1646215 3 804 TM.orf1478 ctaC cytochrome c oxidase,subunit II 1646212 1647162 1 951 TM.orf1479 ctaD cytochrome c oxidase,subunit I 1647159 1648784 2 1626 TM.orf1480 ctaE cytochrome c oxidasesubunit III 1648777 1649412 1 636 TM.orf1481 caa(3)-type oxidase,subunit IV 1649418 1649690 2 273 TM.orf1482 Sulfide: quinoneoxidoreductase 1651323 1649695 −1 1629 TM.orf1483 transmembrane protein1652416 1651634 −3 783 TM.orf1484 bigR transcriptional regulator, ArsRfamily 1652914 1652513 −3 402 TM.orf1485 peroxiredoxin 1653579 1652911−1 669 TM.orf1486 azlB alanine catabolic operon transcriptional 16541541653687 −2 468 regulator protein TM.orf1487 eamB amino acid transporterLysE 1654270 1654920 1 651 TM.orf1488 conserved hypothetical protein1655033 1656364 3 1332 TM.orf1489 yfkF major facilitator superfamilyMFS-1 1656361 1657545 1 1185 TM.orf1490 GATS-like protein 1 16579391657559 −3 381 TM.orf1491 peroxidase-related enzyme 1658554 1657970 −3585 TM.orf1492 yhdH zinc-binding alcohol dehydrogenase 1659600 1658608−1 993 TM.orf1493 dhaR TetR family transcriptional regulator 16603091659629 −3 681 TM.orf1494 nahR LysR family transcriptional regulator1661260 1660334 −3 927 TM.orf1495 conserved hypothetical protein 16613511661824 3 474 TM.orf1496 Alcohol dehydrogenase zinc-binding domain1661821 1662735 1 915 protein TM.orf1497 hypothetical protein 16628431663415 2 573 TM.orf1498 yozG XRE family transcriptional regulator1663415 1663621 3 207 TM.orf1499 yfcG glutathione S-transferasedomain-containing 1664340 1663633 −1 708 protein TM.orf1500 conservedhypothetical protein 1665756 1664476 −1 1281 TM.orf1501 ybfLtransposase, is4 family 1665938 1667053 3 1116 TM.orf1502 ATP-dependentmetalloprotease FtsH 1667172 1668986 2 1815 TM.orf1503 adhA zinc-bindingalcohol dehydrogenase family 1669071 1670054 2 984 protein TM.orf1504dihydroxy-acid dehydratase 1670168 1671841 3 1674 TM.orf1505 xthAexodeoxyribonuclease III Xth 1672701 1671868 −1 834 TM.orf1506 erpAIron-sulfur cluster insertion protein 1673106 1672717 −1 390 TM.orf1507dGTP triphosphohydrolase 1673308 1674513 1 1206 TM.orf1508 argSarginyl-tRNA synthetase 1674515 1676260 3 1746 TM.orf1509 protein TonB,putative 1676260 1677294 1 1035 TM.orf1510 nagZBeta-N-acetylhexosaminidase 1677291 1678337 2 1047 TM.orf1511 scpAcondensin subunit ScpA 1678359 1679288 2 930 TM.orf1512 scpBtranscription regulator 1679278 1679955 1 678 TM.orf1513 membrane-fusionprotein 1680119 1681375 3 1257 TM.orf1514 lagD ATP-binding cassettesubfamily C 1681372 1683636 1 2265 TM.orf1515 hlyB ATP-binding cassettesubfamily B 1683633 1686644 2 3012 TM.orf1516 conserved hypotheticalprotein 1687259 1686669 −2 591 TM.orf1517 bepC type I secretion outermembrane protein, TolC 1688680 1687286 −3 1395 family TM.orf1518hypothetical protein 1688979 1688677 −1 303 TM.orf1519 conservedhypothetical protein 1689357 1688986 −1 372 TM.orf1520 arcA Responseregulators consisting of a CheY-like 1689771 1690445 2 675 receiverdomain and a winged-helix DNA- binding domain TM.orf1521 hypotheticalprotein 1690822 1691073 1 252 TM.orf1522 PMP3 Plasma membraneproteolipid 1691208 1691393 2 186 TM.orf1523 putative proteasome-typeprotease 1692190 1691471 −3 720 TM.orf1524 transglutaminase domainprotein 1693098 1692262 −1 837 TM.orf1525 conserved hypothetical protein1694101 1693154 −3 948 TM.orf1526 conserved hypothetical protein 16957371694187 −2 1551 TM.orf1527 putative anti-sigma regulatory factor,1696399 1695950 −3 450 serine/threonine protein kinase TM.orf1528 btrVanti-anti-sigma regulatory factor 1696803 1696468 −1 336 TM.orf1529 yqeWNa+/Picotransporter 1697053 1698705 1 1653 TM.orf1530 yoaE terC-likemembrane protein 1698858 1699607 2 750 TM.orf1531 hemolysin 16996041700917 3 1314 TM.orf1532 hypothetical protein 1701280 1700933 −3 348TM.orf1533 extracellular solute-binding protein, family 1 17016531702708 3 1056 TM.orf1534 fbpB Fe(3+)-transport system permease protein1702922 1704649 3 1728 sfuB TM.orf1535 ABC transporter related protein1704649 1705710 1 1062 TM.orf1536 tatA Sec-independent proteintranslocase protein 1705796 1706044 3 249 tatA/E homolog TM.orf1537 tatBSec-independent protein translocase protein 1706101 1706607 1 507 TatBTM.orf1538 tatC sec-independent protein translocase protein 17066141707447 1 834 TM.orf1539 serS seryl-tRNA synthetase 1707547 1708836 11290 TM.orf1540 surE acid phosphatase 1708986 1709741 2 756 TM.orf1541pcm Protein-L-isoaspartate(D-aspartate) O- 1709744 1710409 3 666methyltransferase TM.orf1542 ygeR Membrane protein 1710498 1711802 21305 TM.orf1543 fabF 3-oxoacyl-(acyl-carrier-protein) synthase II1713106 1711841 −3 1266 protein TM.orf1544 transcriptional regulator,TetR family 1713711 1713118 −1 594 TM.orf1545 conserved hypotheticalprotein 1714692 1713799 −1 894 TM.orf1546 yajC preprotein translocasesubunit YajC 1714946 1715341 3 396 TM.orf1547 secD Preproteintranslocase subunit SecD 1715451 1717019 2 1569 TM.orf1548 secFPreprotein translocase subunit SecF 1717039 1718007 1 969 TM.orf1549NADH dehydrogenase [ubiquinone] 1 alpha 1718056 1718439 1 384 subcomplexassembly factor TM.orf1550 sodB superoxide dismutase 1719123 1718524 −1600 TM.orf1551 conserved hypothetical protein 1720699 1719497 −3 1203TM.orf1552 crtB fusion protein of y4aC and y4aD 1722519 1720735 −1 1785TM.orf1553 squalene 1722679 1723536 1 858 TM.orf1554 glucose-inhibiteddivision protein A 1724921 1723554 −2 1368 TM.orf1555 hypotheticalprotein 1725496 1725029 −3 468 TM.orf1556 uvrA excinuclease ABC subunitA 1728736 1725845 −3 2892 TM.orf1557 Methyl-accepting chemotaxis proteinsignaling 1730575 1728926 −3 1650 domain TM.orf1558 conservedhypothetical protein 1731388 1730708 −3 681 TM.orf1559 yodQacetylornithine deacetylase 1732898 1731552 −2 1347 TM.orf1560 cmpR LysRfamily transcriptional regulator 1733016 1733912 2 897 TM.orf1561 cysWsulfate ABC transporter, permease protein 1734802 1733906 −3 897 CysWTM.orf1562 cysU sulfate ABC transporter, permease protein 17356661734827 −3 840 CysT TM.orf1563 cysP sulfate ABC transporter,sulfate-binding 1736684 1735674 −2 1011 protein TM.orf1564 hypotheticalprotein 1736794 1736681 −3 114 TM.orf1565 beta-lactamasedomain-containing protein 1738310 1736901 −2 1410 TM.orf1566 ssbsingle-strand DNA binding protein 1738492 1739133 1 642 TM.orf1567aldo/keto reductase 1740203 1739235 −2 969 TM.orf1568 ohrRtranscriptional regulator, MarR family 1740407 1740874 3 468 TM.orf1569ohr organic hydroperoxide resistance protein 1741000 1741413 1 414TM.orf1570 gyrA Type IIA topoisomerase (DNA gyrase/topo II, 17416741744610 2 2937 topoisomerase IV), A subunit TM.orf1571 coaDphosphopantetheine adenylyltransferase 1744632 1745171 2 540 TM.orf1572queA Queuosine biosynthesis protein 1745280 1746341 2 1062 TM.orf1573tgt Queuine/archaeosinetRNA-ribosyltransferase 1746338 1747522 3 1185TM.orf1574 AMP-dependent synthetase and ligase 1749280 1747559 −3 1722TM.orf1575 mcp2 methyl-accepting chemotaxis protein 1750175 1751881 31707 TM.orf1576 hypothetical protein 1752014 1752364 3 351 TM.orf1577similar to protein conserved in bacteria with a 1752687 1753322 2 636cystatin-like fold TM.orf1578 Aldehyde Dehydrogenase 1754996 1753404 −21593 TM.orf1579 Hydroxypyruvate isomerase 1755861 1755070 −1 792TM.orf1580 SprT protein 1755977 1756609 3 633 TM.orf1581 ldc Orn/DAP/Argdecarboxylase 2 1757015 1758154 3 1140 TM.orf1582 haloacid dehalogenase,type II 1758963 1758262 −1 702 TM.orf1583 slt soluble lytic mureintransglycosylase precursor 1761198 1759060 −1 2139 TM.orf1584 dapAdihydrodipicolinate synthase 1761583 1762494 1 912 TM.orf1585 smpBSsrA-binding protein 1762550 1763026 3 477 TM.orf1586 yuiHoxidoreductase 1763031 1763738 2 708 TM.orf1587 putative uracil-DNAglycosylase 1764448 1763729 −3 720 TM.orf1588 conserved hypotheticalprotein 1765409 1764588 −2 822 TM.orf1589 folK7,8-Dihydro-6-hydroxymethylpterin- 1765659 1766156 2 498pyrophosphokinase, HPPK TM.orf1590 rpoZ DNA-directed RNA polymerase,omega 1766263 1766664 1 402 subunit TM.orf1591 rsh Guanosinepolyphosphate 1766844 1769000 2 2157 pyrophosphohydrolases/synthetasesTM.orf1592 pyrE orotate phosphoribosyltransferase 1769087 1769689 3 603TM.orf1593 pdxJ Pyridoxal phosphate biosynthesis protein 1769762 17705533 792 TM.orf1594 acpS 4′-phosphopantetheinyl transferase 1770558 17709892 432 TM.orf1595 lepB signal peptidase I 1770986 1771786 3 801TM.orf1596 rnc RNAse III 1771856 1772584 3 729 TM.orf1597 eraGTP-binding protein Era 1772581 1773660 1 1080 TM.orf1598 hypotheticalprotein 1773946 1774569 1 624 TM.orf1599 p040 1774559 1775173 3 615TM.orf1600 hypothetical protein 1775189 1775713 3 525 TM.orf1601conserved hypothetical protein 1776669 1775686 −1 984 TM.orf1602transcriptional regulator protein 1776764 1777684 3 921 TM.orf1603 recODNA repair protein RecO 1777681 1778469 1 789 TM.orf1604 parC Gramnegative topoisomerase IV, subunit A 1778466 1780784 2 2319 TM.orf1605TRAP dicarboxylate transporter, DctM subunit 1782490 1780913 −3 1578TM.orf1606 tripartite ATP-independent periplasmic 1782764 1782495 −2 270transporter DctQ TM.orf1607 tripartite ATP-independent periplasmic1783036 1782752 −3 285 transporter DctQ TM.orf1608 Bacterialextracellular solute-binding protein, 1784565 1783456 −1 1110 family 7TM.orf1609 TRAP-type transports system extracellular 1786091 1784991 −21101 solute binding protein TM.orf1610 Bacterial extracellularsolute-binding protein, 1787389 1786277 −3 1113 family 7 TM.orf1611 ateArginyltransferase 1788423 1787695 −1 729 TM.orf1612 tdcB threoninedehydratase 1789781 1788525 −2 1257 TM.orf1613 quiP peptidase S45penicillin amidase 1792263 1789819 −1 2445 TM.orf1614 Odc1 lysine1792435 1793697 1 1263 TM.orf1615 mhpD fumarylacetoacetate (FAA)hydrolase 1794703 1793690 −3 1014 TM.orf1616 hemB Delta-aminolevulinicacid dehydratase 1795758 1794754 −1 1005 TM.orf1617 putative3-hydroxyisobutyryl-Coenzyme A 1796022 1797101 2 1080 hydrolaseTM.orf1618 expG transcriptional regulator 1797343 1797798 1 456TM.orf1619 speE spermidine synthase 1798767 1797889 −1 879 TM.orf1620speH S-adenosylmethioninedecarboxylase 1799218 1798817 −3 402 proenzymeTM.orf1621 Haloacetate dehalogenase H-1 1799576 1800466 3 891 TM.orf1622conserved hypothetical protein 1800503 1801288 3 786 TM.orf1623hypothetical protein 1801610 1802128 3 519 TM.orf1624 rnk nucleosidediphosphate kinase regulator 1802160 1802576 2 417 TM.orf1625 recGATP-dependent DNA helicase 1804676 1802577 −2 2100 TM.orf1626 EMI5Succinate dehydrogenase assembly factor 1804861 1805169 1 309 TM.orf1627mfd transcription-repair coupling factor superfamily 1805326 1808889 13564 II helicase TM.orf1628 conserved hypothetical protein 18090611809393 1 333 TM.orf1629 hypothetical protein 1809447 1809929 2 483TM.orf1630 pleD response regulator/GGDEF domain protein 1811242 1809914−3 1329 TM.orf1631 ytcI AMP-dependent synthetase and ligase 18128101811293 −1 1518 TM.orf1632 methyl-accepting chemotaxis protein 18152151813104 −2 2112 TM.orf1633 tRNA/rRNA methyltransferase (SpoU) 18155831816182 1 600 TM.orf1634 pilJ methyl-accepting chemotaxis protein1818315 1816198 −1 2118 TM.orf1635 Transmembrane protein 1819276 1818431−3 846 TM.orf1636 Glycosyltransferase 1820451 1819339 −1 1113 TM.orf1637UDP-2,3-diacylglucosamine hydrolase 1821447 1820455 −1 993 TM.orf1638cold-shock DNA-binding domain-containing 1821901 1821695 −3 207 proteinTM.orf1639 cold-shock DNA-binding domain protein 1822480 1822274 −3 207TM.orf1640 kipR regulatory protein, IclR 1823933 1823052 −2 882TM.orf1641 bcpA 2,3-dimethylmalate lyase 1824793 1823930 −3 864TM.orf1642 putative branched chain amino acid ABC 1826112 1824880 −11233 transporter substrate-binding protein TM.orf1643 IivF ABCtransporter related protein 1826920 1826201 −3 720 TM.orf1644 braFHigh-affinity branched-chain amino acid 1827752 1826913 −2 840 transportATP-binding protein braF TM.orf1645 IivM branched chain amino acid ABCtransporter 1828717 1827749 −3 969 inner membrane protein TM.orf1646IivH putative ABC transporter, permease protein 1829599 1828733 −3 867TM.orf1647 leuD 3-isopropylmalate dehydratase, small subunit 18301341829604 −2 531 TM.orf1648 3-isopropylmalate dehydratase large subunit1831449 1830193 −1 1257 TM.orf1649 F0F1 ATP synthase subunit beta1831718 1833145 3 1428 TM.orf1650 alternate F1F0 ATPase, F1 subunitepsilon 1833142 1833570 1 429 TM.orf1651 F0F1-ATPase subunit 18335871833877 3 291 TM.orf1652 hypothetical protein 1833874 1834194 1 321TM.orf1653 F0F1 ATP synthase subunit A 1834191 1834925 2 735 TM.orf1654F0F1 ATP synthase subunit C 1834922 1835170 3 249 TM.orf1655 H +transporting two-sector ATPase B/B′ 1835184 1835927 2 744 subunitTM.orf1656 F0F1 ATP synthase subunit alpha 1835914 1837476 1 1563TM.orf1657 atpG H + transporting two-sector ATPase gamma 1837473 18383542 882 subunit TM.orf1658 hypothetical protein 1838654 1838367 −2 288TM.orf1659 major facilitator superfamily MFS_1 1840067 1838769 −2 1299TM.orf1660 TfoX domain-containing protein 1840283 1840675 3 393TM.orf1661 conserved hypothetical protein 1840779 1842725 2 1947TM.orf1662 ppdK Pyruvate, phosphate dikinase 1842737 1844335 3 1599TM.orf1663 yehW binding-protein-dependent transport systems 18450881844321 −3 768 inner membrane component TM.orf1664 yehX ABC transporterrelated protein 1846020 1845085 −1 936 TM.orf1665 yehYbinding-protein-dependent transport systems 1847249 1846017 −2 1233inner membrane component TM.orf1666 osmF Substrate-binding region ofABC-type glycine 1848202 1847246 −3 957 betaine transport systemTM.orf1667 putative membrane protein 1848844 1848488 −3 357 TM.orf1668response regulator 1849103 1850494 3 1392 TM.orf1669 lipolytic enzyme1851479 1850499 −2 981 TM.orf1670 vapI XRE family plasmid maintenancesystem 1851988 1852281 1 294 antidote protein TM.orf1671 conservedhypothetical protein 1852615 1852304 −3 312 TM.orf1672 transposase,mutator type 1852717 1853367 1 651 TM.orf1673 transposase, mutator type1853497 1853646 1 150 TM.orf1674 mltB Lytic murein transglycosylase1855361 1856512 3 1152 TM.orf1675 rlpA Lipoproteins 1856642 1857619 3978 TM.orf1676 dacA serine-type D-Ala-D-Ala carboxypeptidase 18576931858931 2 1239 TM.orf1677 tmk thymidylate kinase 1858943 1859620 3 678TM.orf1678 holB ATPase involved in DNA replication 1859617 1860744 11128 TM.orf1679 metG methionyl-tRNA synthetase 1860830 1862380 3 1551TM.orf1680 ycfH TatD-related deoxyribonuclease 1862436 1863242 2 807TM.orf1681 lipB metallo-beta-lactamase superfamily 1863242 1864087 3 846TM.orf1682 hypothetical protein 1864096 1864536 1 441 TM.orf1683regulatory protein TetR 1865132 1864539 −2 594 TM.orf1684 ydgK drugresistance transporter, Bcr/CflA subfamily 1866381 1865194 −1 1188TM.orf1685 conserved hypothetical protein 1866575 1866916 3 342TM.orf1686 rpoE RNA polymerase, sigma-24 subunit, ECF 1866987 1867547 2561 subfamily TM.orf1687 conserved hypothetical protein 1867531 18683011 771 TM.orf1688 conserved hypothetical protein 1868393 1869109 3 717TM.orf1689 conserved hypothetical protein 1869258 1869860 2 603TM.orf1690 acetyltransferase 1870904 1870125 −2 780 TM.orf1691 dnaBreplicative DNA helicase 1871863 1871147 −3 717 TM.orf1692 yvbTconserved hypothetical protein 1872145 1873167 1 1023 TM.orf1693 mazGMazG family protein 1873366 1874289 1 924 TM.orf1694 ydfG short-chaindehydrogenase/reductase SDR 1874289 1874606 2 318 TM.orf1695 ydfGshort-chain dehydrogenase/reductase SDR 1874603 1875043 3 441 TM.orf1696groL unnamed protein product 1876885 1875239 −3 1647 TM.orf1697 groSchaperonin 1877294 1877004 −2 291 TM.orf1698 hflX GTP-binding proteinHFLX 1878805 1877438 −3 1368 TM.orf1699 hfq RNA-binding protein Hfq1879268 1878990 −2 279 TM.orf1700 HAD-superfamily hydrolase, subfamilyIA, 1880150 1879434 −2 717 variant 1 TM.orf1701 trkA Trk systempotassium uptake protein 1881552 1880176 −1 1377 TM.orf1702 ntrXresponse regulator containing CheY-like 1882971 1881586 −1 1386 receiverTM.orf1703 Nitrogen regulation protein, NtrY, Signal 1885423 1882976 −32448 transduction histidine kinase TM.orf1704 ntrC two-componentresponse regulator, nitrogen 1886904 1885420 −1 1485 regulation responseTM.orf1705 nitrogen regulation protein 1888063 1886921 −3 1143TM.orf1706 dus tRNA-dihydrouridine synthase 1889211 1888213 −1 999TM.orf1707 IspD/ispF bifunctional enzyme 1889399 1890658 3 1260TM.orf1708 pgpA phosphatidylglycerophosphatase A and 1890655 1891176 1522 related protein TM.orf1709 ygaD protein (competence- andmitomycin-induced) 1891199 1891690 3 492 TM.orf1710 sigma factor, sigma70 type, group 4 (ECF) 1892690 1891716 −2 975 TM.orf1711 conservedhypothetical protein 1893151 1892687 −3 465 TM.orf1712 oligoketidecyclase/lipid transport protein 1893770 1893270 −2 501 TM.orf1713 lipAlipoic acid synthetase 1894835 1893879 −2 957 TM.orf1714 lpddihydrolipoamide dehydrogenase 1896793 1894970 −3 1824 TM.orf1715pyruvate dehydrogenase complex 1898249 1896852 −2 1398 dihydrolipoamideacetyltransferase TM.orf1716 pdhB Pyruvate dehydrogenase E1 component,beta 1899277 1898291 −3 987 subunit TM.orf1717 pdhA2-dehydro-3-deoxyphosphooctonate aldolase 1900396 1899374 −3 1023TM.orf1718 yybE transcriptional regulator, LysR family 1901444 1900551−2 894 TM.orf1719 putative enoyl-CoA hydratase echA8 1901579 1902382 3804 TM.orf1720 Peptidoglycan-binding domain 1 protein 1903739 1902756 −2984 TM.orf1721 septum formation initiator 1904226 1903840 −1 387TM.orf1722 amiC ABC-type branched-chain amino acid 1904609 1905880 31272 transport system, periplasmic component TM.orf1723 IivHigh-affinity branched-chain amino acid 1906153 1907892 1 1740 transportsystem permease protein IivH TM.orf1724 IivM branched chain amino acidABC transporter 1907897 1909099 3 1203 permease TM.orf1725 braF putativeATP-binding component of ABC 1909096 1909899 1 804 transporterTM.orf1726 IivF ABC transporter, nucleotide binding/ATPase 19099151910610 1 696 protein (urea/amide) TM.orf1727 ureD urease accessoryprotein UreD 1910629 1911585 1 957 TM.orf1728 ureA urease, gamma subunit1911653 1911955 3 303 TM.orf1729 ureB urease subunit beta 19119661912364 2 399 TM.orf1730 ureC Urease alpha subunit 1912369 1914078 11710 TM.orf1731 ureE urease accessory protein 1914190 1915035 1 846TM.orf1732 ureF Urease accessory protein UreF 1915043 1915798 3 756TM.orf1733 ureG urease accessory protein 1915852 1916511 1 660TM.orf1734 conserved hypothetical protein 1917954 1916518 −1 1437TM.orf1735 glycosyl transferase WecB/TagA/CpsF 1918745 1917951 −2 795TM.orf1736 polysaccharide export protein 1919529 1918765 −1 765TM.orf1737 cpsD lipopolysaccharide biosynthesis 1921541 1919529 −2 2013TM.orf1738 conserved hypothetical protein 1922273 1923637 3 1365TM.orf1739 wcaJ sugar transferase 1923693 1925129 2 1437 TM.orf1740glycoside hydrolase family 18 1925129 1926148 3 1020 TM.orf1741 FkbMfamily methyltransferase 1927010 1926171 −2 840 TM.orf1742 hypotheticalprotein 1927033 1927158 1 126 TM.orf1743 conserved hypothetical protein1927269 1927676 2 408 TM.orf1744 dgdR LysR family transcriptionalregulator 1928536 1927688 −3 849 TM.orf1745 Membrane protein 19286911929641 2 951 TM.orf1746 eno enolase 1930955 1929666 −2 1290 TM.orf1747kdsA 2-dehydro-3-deoxyphosphooctonate aldolase 1931957 1931103 −2 855TM.orf1748 pyrG CTP synthase 1933636 1932002 −3 1635 TM.orf1749hypothetical protein 1934265 1933840 −1 426 TM.orf1750 tpiAtriose-phosphate isomerase 1935114 1934356 −1 759 TM.orf1751 ppiDpeptidyl-prolyl cis-trans isomerse 1935472 1937385 1 1914 TM.orf1752trpE Anthranilate synthase component I 1937456 1938973 3 1518 TM.orf1753guaA putative GMP synthase [glutamine- 1938970 1939551 1 582hydrolyzing] TM.orf1754 copZ heavy metal transport/detoxificationprotein 1939783 1939574 −3 210 TM.orf1755 yibQ conserved hypotheticalprotein 1941474 1939789 −1 1686 TM.orf1756 hmrR Cu(I)-responsivetranscriptional regulator 1941993 1941529 −1 465 TM.orf1757 copAcopper-translocating P-type ATPase 1944254 1941990 −2 2265 TM.orf1758trpG anthranilate synthase component II 1944525 1945148 2 624 TM.orf1759trpD anthranilate phosphoribosyltransferase 1945145 1946185 3 1041TM.orf1760 trpC indole-3-glycerol phosphate synthase 1946190 1947005 2816 TM.orf1761 moaC molybdenum cofactor biosynthesis protein 19470111947508 3 498 MoaC TM.orf1762 moeA molybdopterin biosynthesis protein1947547 1948782 1 1236 TM.orf1763 lexA LexA repressor 1949031 1949879 2849 TM.orf1764 ComEC/Rec2-related protein 1952111 1949964 −2 2148TM.orf1765 Glutamyl-tRNA synthetase, class Ic 1952232 1953629 2 1398TM.orf1766 plpC NLPA lipoprotein 1954013 1954648 3 636 TM.orf1767conserved hypothetical protein 1954721 1956109 3 1389 TM.orf1768 lpxBlipid-A-disaccharide synthase 1957339 1956149 −3 1191 TM.orf1769conserved hypothetical protein 1958160 1957336 −1 825 TM.orf1770 lpxAUDP-N-acetylglucosamine acyltransferase 1958996 1958157 −2 840TM.orf1771 fabZ beta-hydroxyacyl-(acyl-carrier-protein) 1959469 1958993−3 477 dehydratase FabZ TM.orf1772 lpxD UDP-3-O-[3-hydroxymyristoyl]glucosamine N- 1960565 1959534 −2 1032 acyltransferase TM.orf1773 outermembrane protein 1961264 1960677 −2 588 TM.orf1774 yaeT outer membraneprotein 1963560 1961269 −1 2292 TM.orf1775 zinc metalloprotease Atu13801964840 1963683 −2 1158 TM.orf1776 dxr 1-deoxy-D-xylulose 5-phosphate1965945 1964890 −1 1056 reductoisomerase TM.orf1777 cdsA CDP-diglyceridesynthetase 1966871 1966098 −2 774 TM.orf1778 uppS undecaprenylpyrophosphate synthetase 1967679 1966966 −1 714 TM.orf1779 frr ribosomerecycling factor 1968277 1967717 −3 561 TM.orf1780 pyrH uridylate kinase1969009 1968281 −3 729 TM.orf1781 tsf elongation factor Ts 19701951969272 −2 924 TM.orf1782 rpsB 30S ribosomal protein S2 1971239 1970433−2 807 TM.orf1783 DNA polymerase III alpha subunit 1975030 1971530 −33501 TM.orf1784 conserved hypothetical protein 1975621 1975127 −3 495TM.orf1785 conserved hypothetical protein 1976125 1975841 −3 285TM.orf1786 ptxR LysR family transcriptional regulator 1977125 1976223 −2903 TM.orf1787 nylA 6-aminohexanoate-cyclic-dimer hydrolase 19786241977197 −3 1428 TM.orf1788 nreC transcriptional regulator, LuxR family1979695 1978703 −3 993 TM.orf1789 rpsI 30S ribosomal protein S9 19803331979839 −1 495 TM.orf1790 rplM Ribosomal protein L13 1980799 1980341 −3459 TM.orf1791 Enoyl-CoA hydratase/carnithine racemase 1981104 1981913 2810 TM.orf1792 yccU O-acetylhomoserine/O-acetylserine 1981918 1982421 1504 sulfhydrylase TM.orf1793 cysD O-acetylhomoserine sulfhydrylase1982418 1983734 2 1317 TM.orf1794 phoE phosphoglycerate mutase familyprotein 1983773 1984378 3 606 TM.orf1795 azoR FMN-dependentNADH-azoreductase 1985030 1984401 −2 630 TM.orf1796 transcriptionalregulator, LysR family 1985165 1986124 3 960 TM.orf1797 flavinreductase-like, FMN-binding 1986115 1986681 1 567 TM.orf1798 dgdRtranscriptional regulator, LysR family protein 1987558 1986707 −3 852TM.orf1799 dgdA aminotransferase class-Ill 1987691 1988995 3 1305TM.orf1800 conserved hypothetical protein 1989035 1989901 3 867TM.orf1801 glutamine amidotransferase, class 1989950 1991113 3 1164II/dipeptidase TM.orf1802 conserved hypothetical protein 1991182 19922881 1107 TM.orf1803 TRAP-T family transporter, DctQ (4 TMs) 19923011992771 1 471 subunit TM.orf1804 TRAP-T family protein transporter, DctM(12 1992768 1994075 2 1308 TMs) subunit TM.orf1805 conservedhypothetical protein 1995126 1994083 −1 1044 TM.orf1806 HD superfamilymetal-dependent 1996391 1995123 −2 1269 phosphohydrolase TM.orf1807divalent cation tolerance protein 1997241 1996522 −1 720 TM.orf1808transcriptional regulator, MerR family 1999060 1998188 −3 873 TM.orf1809ihfA integration host factor subunit alpha 1999445 1999101 −2 345TM.orf1810 fabH 3-oxoacyl-[acyl-carrier-protein] synthase III 20005831999606 −1 978 TM.orf1811 plsX putative glycerol-3-phosphateacyltransferase 2001644 2000580 −2 1065 PlsX TM.orf1812 rpmF Ribosomalprotein L32 2001904 2001719 −3 186 TM.orf1813 conserved hypotheticalprotein 2002635 2002018 −1 618 TM.orf1814 uqcc ubiquinol-cytochrome Cchaperone 2003360 2002755 −2 606 TM.orf1815 conserved hypotheticalprotein 2003577 2004143 2 567 TM.orf1816 hypothetical protein 20046902004232 −1 459 TM.orf1817 conserved hypothetical protein 2005305 2004736−1 570 TM.orf1818 thiL thiamine-monophosphate kinase 2006528 2005488 −21041 TM.orf1819 nusB transcription antitermination protein NusB 20071182006525 −3 594 TM.orf1820 6,7-dimethyl-8-ribityllumazine synthase2007584 2007135 −2 450 TM.orf1821 GTP cyclohydrolase II 2008743 2007592−1 1152 TM.orf1822 ribE riboflavin synthase, alpha subunit 20094252008793 −3 633 TM.orf1823 ribD riboflavin biosynthesis protein ribD2010538 2009504 −3 1035 TM.orf1824 Transcriptional repressor nrdR2011124 2010648 −2 477 TM.orf1825 glyA serine hydroxymethyltransferase2012503 2011205 −3 1299 TM.orf1826 ywlF ribose 5-phosphate isomeraseRpiB 2013101 2012658 −2 444 TM.orf1827 transcriptional regulatoryprotein MucR 2013596 2014069 3 474 TM.orf1828 hypothetical protein2014465 2014229 −3 237 TM.orf1829 hypothetical protein 2015327 2014479−2 849 TM.orf1830 hypothetical protein 2016262 2015324 −3 939 TM.orf1831hypothetical protein 2016565 2016266 −3 300 TM.orf1832 conservedhypothetical protein 2016880 2016608 −3 273 TM.orf1833 aspSAspartyl-tRNA synthetase 2018935 2017109 −3 1827 TM.orf1834 rndRibonuclease D 2019195 2020463 2 1269 TM.orf1835 putativeglyoxalase/bleomycin resistance 2021259 2020492 −1 768protein/dioxygenase family protein TM.orf1836 chromosomal replicationinitiator 2021912 2021256 −2 657 TM.orf1837 permease 2023120 2021981 −31140 TM.orf1838 CDP-alcohol phosphatidyltransferase 2023757 2023140 −2618 TM.orf1839 conserved hypothetical protein 2025005 2023761 −2 1245TM.orf1840 purM Phosphoribosylformylglycinamidine cyclo- 2025286 20264191 1134 ligase TM.orf1841 GART phosphoribosylglycinamideformyltransferase 2026392 2027057 2 666 TM.orf1842 ndk Nucleosidediphosphate kinase 2027572 2027150 −3 423 TM.orf1843 yheS ABCtransporter, ATP-binding protein 2027829 2029742 2 1914 TM.orf1844 walKPAS/PAC sensor hybrid histidine kinase 2030545 2032674 1 2130 TM.orf1845DNA polymerase III subunit chi 2033165 2032686 −2 480 TM.orf1846 pepAleucyl aminopeptidase 2034667 2033183 −3 1485 TM.orf1847 Predictedpermeases 2034999 2036126 2 1128 TM.orf1848 putative permease 20361232037277 3 1155 TM.orf1849 lptD Organic solvent tolerance protein OstA2037280 2039472 1 2193 TM.orf1850 surA Parvulin-like peptidyl-prolylisomerase 2039504 2040784 3 1281 TM.orf1851 pdxA dimethyladenosinetransferase 2040771 2041805 2 1035 TM.orf1852 rsmA dimethyladenosinetransferase 2041853 2042728 3 876 TM.orf1853 yrbG sodium/calciumexchanger 2042829 2043818 2 990 TM.orf1854 gmk Guanylate kinase (GMPkinase) 2044522 2043848 −3 675 TM.orf1855 yicC stress-induced protein2045441 2044551 −2 891 TM.orf1856 Short-chain dehydrogenase/reductase(SDR) 2045603 2046376 3 774 superfamily TM.orf1857 btuB TonB-dependentreceptor 2046799 2048769 1 1971 TM.orf1858 periplasmic binding protein2048773 2049651 1 879 TM.orf1859 yvrB transport system permease protein2049648 2050643 2 996 TM.orf1860 fecE iron(III) dicitrate transportATP-binding protein 2050647 2051492 2 846 FecE TM.orf1861 conservedhypothetical protein 2051447 2052490 3 1044 TM.orf1862 periplasmicsolute-binding protein 2053517 2052495 −2 1023 TM.orf1863 fabF3-oxoacyl-[acyl-carrier-protein] synthase II 2054827 2053568 −3 1260TM.orf1864 acpP transit peptide-acyl carrier protein fusion 20553482055112 −1 237 protein TM.orf1865 nodG dehydrogenase 2056285 2055548 −3738 TM.orf1866 fabD S-malonyltransferase 2057284 2056343 −3 942TM.orf1867 hypothetical protein 2057425 2057538 1 114 TM.orf1868 rpsF30S ribosomal protein S6 2057880 2058332 2 453 TM.orf1869 rpsR smallsubunit ribosomal protein S18 2058365 2058640 3 276 TM.orf1870 conservedhypothetical protein 2058801 2059754 2 954 TM.orf1871 rplI 50S ribosomalprotein L9 2059777 2060346 1 570 TM.orf1872 cfacyclopropane-fatty-acyl-phospholipid synthase 2060777 2062018 3 1242TM.orf1873 dnaB replicative DNA helicase 2062180 2063763 1 1584TM.orf1874 alr alanine racemase 2063760 2064986 2 1227 TM.orf1875putative ABC transporter permease protein 2064983 2065756 3 774TM.orf1876 putative ribonucleotide transport ATP-binding 2065791 20665702 780 protein TM.orf1877 radA DNA repair protein RadA 2066607 2068079 21473 TM.orf1878 cvpA CvpA family protein 2068136 2068903 3 768TM.orf1879 purF amidophosphoribosyltransferase 2068966 2070456 1 1491TM.orf1880 yciK short-chain dehydrogenase/reductase SDR 2070503 20713273 825 TM.orf1881 regulatory protein TetR 2072065 2071397 −3 669TM.orf1882 hypothetical protein 2072479 2072607 1 129 TM.orf1883 SRY1amino-acid dehydratase protein 2073857 2072868 −2 990 TM.orf1884 engAGTP-binding protein EngA 2075421 2073862 −1 1560 TM.orf1885 yfgL PQQenzyme repeat family protein 2076972 2075596 −1 1377 TM.orf1886conserved hypothetical protein 2077694 2076969 −2 726 TM.orf1887 2′-5′RNA ligase 2079212 2079949 3 738 TM.orf1888 alpha/beta fold familyhydrolase 2079217 2077826 −3 1392 TM.orf1889 conserved hypotheticalprotein 2080076 2081422 3 1347 TM.orf1890 qor oxidoreductase,zinc-binding dehydrogenase 2082477 2081425 −1 1053 family TM.orf1891virF transcriptional regulator, AraC family 2083450 2082566 −3 885TM.orf1892 pbuA TonB-dependent siderophore receptor 2083700 2085835 32136 TM.orf1893 conserved hypothetical protein 2085867 2086160 2 294TM.orf1894 alkB alkylated DNA repair protein 2086811 2086161 −2 651TM.orf1895 conserved hypothetical protein 2087542 2086829 −3 714TM.orf1896 ttuE pyruvate kinase 2089060 2087633 −3 1428 TM.orf1897putative helicase/glycosylase 2090031 2089057 −1 975 TM.orf1898conserved hypothetical protein 2091276 2090047 −1 1230 TM.orf1899 Zinctransporter 2092271 2091309 −2 963 TM.orf1900 sldA glucose dehydrogenase2094700 2092283 −3 2418 TM.orf1901 quinolinate synthetase complex, Asubunit 2094913 2095887 1 975 TM.orf1902 nadB L-aspartate oxidase2095893 2097410 2 1518 TM.orf1903 nadC nicotinate-nucleotidepyrophosphorylase 2097397 2098254 1 858 TM.orf1904 multi-sensor signaltransduction histidine 2098362 2099126 2 765 kinase TM.orf1905 rpfGputative PAS/PAC sensor protein 2099123 2099464 3 342 TM.orf1906SMP-30/Gluconolaconase/LRE domain- 2099618 2100664 3 1047 containingprotein TM.orf1907 conserved hypothetical protein 2101439 2100675 −2 765TM.orf1908 valS Valyl-tRNA synthetase 2104205 2101536 −2 2670 TM.orf1909conserved hypothetical protein 2105076 2104375 −1 702 TM.orf1910 bepCTolC family type I secretion outer membrane 2106603 2105257 −1 1347protein TM.orf1911 pcm2 protein-L-isoaspartate(D-aspartate) O- 21073572106701 −3 657 methyltransferase TM.orf1912 hypothetical protein 21078152107594 −1 222 TM.orf1913 alkK medium-chain-fatty-acid--CoA ligase2108418 2110052 2 1635 TM.orf1914 SCP2 sterol carrier family protein2110578 2110285 −1 294 TM.orf1915 dhkK multi-sensor hybrid histidinekinase 2110886 2113477 3 2592 TM.orf1916 conserved hypothetical protein2114158 2113478 −3 681 TM.orf1917 pulE general secretion pathway proteinE 2114300 2115988 3 1689 TM.orf1918 hypothetical protein 2115985 21172801 1296 TM.orf1919 hypothetical protein 2117277 2118068 2 792 TM.orf1920hypothetical protein 2118220 2118795 1 576 TM.orf1921 hypotheticalprotein 2118990 2120030 2 1041 TM.orf1922 outD type II and III secretionsystem protein 2120027 2121799 3 1773 TM.orf1923 conserved hypotheticalprotein 2121796 2122551 1 756 TM.orf1924 pleC Sensor protein 21261812122729 −1 3453 TM.orf1925 elongation factor G 2128635 2126509 −1 2127TM.orf1926 hypothetical protein 2129444 2130598 3 1155 TM.orf1927 imuBnucleotidyltransferase/DNA polymerase 2130673 2132406 1 1734 involved inDNA repair TM.orf1928 DNA polymerase III subunit alpha 2132460 2135711 23252 TM.orf1929 phospholipid N-methyltransferase 2135982 2136569 2 588TM.orf1930 pdhS Sensor protein 2138390 2136588 −2 1803 TM.orf1931 SpoVRfamily protein 2140127 2138673 −2 1455 TM.orf1932 conserved hypotheticalprotein 2141480 2140197 −2 1284 TM.orf1933 prkA PrkA serine kinase2143509 2141578 −1 1932 TM.orf1934 hypothetical protein 2144138 2143938−2 201 TM.orf1935 hypothetical protein 2144325 2144131 −1 195 TM.orf1936GNAT family acetyltransferase 2144648 2144277 −2 372 TM.orf1937conserved hypothetical protein 2145649 2144921 −3 729 TM.orf1938periplasmic binding protein 2146284 2147204 2 921 TM.orf1939 yfiZtransport system permease protein 2147201 2148211 3 1011 TM.orf1940 yclPABC transporter related protein 2148205 2148993 1 789 TM.orf1941 recJsingle-stranded-DNA-specificexonuclease 2150814 2149024 −1 1791TM.orf1942 ywjI fructose 1,6-bisphosphatase II 2151844 2150867 −3 978TM.orf1943 hom homoserine dehydrogenase 2153434 2152106 −3 1329TM.orf1944 aatC aminotransferase 2154657 2153431 −1 1227 TM.orf1945 phbCpoly-beta-hydroxybutyrate polymerase 2155556 2157358 3 1803 TM.orf1946yeiE LysR family transcriptional regulator 2158296 2157418 −1 879TM.orf1947 ydgK drug resistance transporter, Bcr/CflA subfamily 21595192158293 −2 1227 TM.orf1948 membrane protein 2160635 2159586 −2 1050TM.orf1949 hexapaptide repeat-containing transferase 2161402 2160773 −3630 TM.orf1950 lolD ABC transporter related protein 2162133 2161450 −1684 TM.orf1951 lolC lipoprotein-releasing system permease protein2163373 2162126 −3 1248 TM.orf1952 pcmProtein-L-isoaspartate(D-aspartate) O- 2163558 2164793 2 1236methyltransferase (PCMT) family TM.orf1953 conserved hypotheticalprotein 2165229 2164807 −1 423 TM.orf1954 xanthine/uracil/vitamin Cpermease 2165468 2166760 3 1293 TM.orf1955 yeaM AraC familytranscriptional regulator 2168105 2167317 −2 789 TM.orf1956 qacA MFSfamily transporter 2168260 2169447 1 1188 TM.orf1957 CRYZ Alcoholdehydrogenase zinc-binding domain 2170488 2169475 −1 1014 proteinTM.orf1958 pecT lysR-like transcriptional regulator 2170607 2171488 3882 TM.orf1959 rhtC lysine exporter family protein 2171560 2172219 1 660TM.orf1960 conserved hypothetical protein 2172465 2172226 −1 240TM.orf1961 methylmalonyl-CoA epimerase 2172974 2172570 −2 405 TM.orf1962rnjA beta-lactamase domain-containing protein 2174803 2173139 −3 1665TM.orf1963 coaX pantothenate kinase 2175615 2174836 −1 780 TM.orf1964biotin- 2176456 2175644 −3 813 TM.orf1965 nuoN NADH dehydrogenasesubunit N 2177898 2176453 −1 1446 TM.orf1966 nuoM NADH dehydrogenasesubunit M 2179443 2177920 −1 1524 TM.orf1967 NADH dehydrogenase subunitL 2181374 2179443 −2 1932 TM.orf1968 nuoK NADH dehydrogenase subunit K2181690 2181382 −1 309 TM.orf1969 nuoJ NADH-quinone oxidoreductase chain10 2182304 2181690 −2 615 TM.orf1970 nuoI NADH dehydrogenase subunit I2182900 2182412 −3 489 TM.orf1971 nuoH NADH dehydrogenase subunit G2184067 2183054 −3 1014 TM.orf1972 NADH-quinone oxidoreductase, chain G2186138 2184060 −2 2079 TM.orf1973 NADH dehydrogenase I subunit F2187429 2186149 −1 1281 TM.orf1974 NADH dehydrogenase I chain E 21880452187419 −3 627 TM.orf1975 nuoD NADH dehydrogenase I chain D 21892472188060 −1 1188 TM.orf1976 nuoC NADH (or F420H2) dehydrogenase, subunitC 2189798 2189244 −2 555 TM.orf1977 nuoB NADH-quinone oxidoreductase, Bsubunit 2190524 2189967 −2 558 TM.orf1978 NADH-ubiquinone/plastoquinone2190886 2190521 −3 366 oxidoreductase, chain 3 TM.orf1979 putativeflavin-binding monooxygenase 2193069 2191516 −1 1554 TM.orf1980 huphistone-like DNA-binding protein 2193901 2193626 −3 276 TM.orf1981 lonATP-dependent protease La 2196599 2194116 −2 2484 TM.orf1982 clpXATP-dependent protease ATP-binding subunit 2198022 2196754 −1 1269TM.orf1983 clpP Protease subunit of ATP-dependent Clp 2199088 2198456 −3633 proteases TM.orf1984 tig FKBP-type peptidyl-prolyl cis-transisomerase 2200572 2199214 −1 1359 (trigger factor) TM.orf1985 conservedhypothetical protein 2202443 2200824 −2 1620 TM.orf1986 exoZexopolysaccharide production 2203626 2202583 −1 1044 (acetyltransferase)protein TM.orf1987 malonyl-CoA decarboxylase 2205347 2203839 −2 1509TM.orf1988 conserved hypothetical protein 2207017 2205452 −3 1566TM.orf1989 hypothetical protein 2207192 2207560 3 369 TM.orf1990endonuclease/exonuclease/phosphatase 2207572 2208174 1 603 TM.orf1991ybdL possible cysteine-S-conjugate beta-lyase 2209649 2208462 −2 1188TM.orf1992 glnB Nitrogen regulatory protein P-II 2210083 2210421 1 339TM.orf1993 glnA L-glutamine synthetase 2210677 2212086 1 1410 TM.orf1994conserved hypothetical protein 2213194 2212262 −3 933 TM.orf1995 lysRtranscriptional regulator, LysR family 2213332 2214294 1 963 TM.orf1996parE topoisomerase IV subunit B 2214825 2216804 2 1980 TM.orf1997hypothetical protein 2216898 2217503 2 606 TM.orf1998 hypotheticalprotein 2217551 2218081 3 531 TM.orf1999 hypothetical protein 22181052218752 1 648 TM.orf2000 hypothetical protein 2218981 2218829 −3 153TM.orf2001 chemotaxis sensory transducer 2219272 2220630 1 1359TM.orf2002 hypothetical protein 2220928 2220659 −3 270 TM.orf2003 phnMputative metal-dependent hydrolase involved 2220969 2221925 2 957 inphosphonate metabolism; PhnM protein TM.orf2004 bioH hydrolase, alpha2222639 2221926 −2 714 TM.orf2005 isochorismatase domain-containingprotein 2A 2223264 2222671 −1 594 TM.orf2006 rhlE DeaD 2223380 2224945 31566 TM.orf2007 hydrolase or acyltransferase 2226033 2225167 −1 867TM.orf2008 putative amino acid or sugar ABC transport 2227092 2226037 −11056 system, permease protein TM.orf2009 braD putative amino acid orsugar ABC transport 2228115 2227108 −1 1008 system, permease proteinTM.orf2010 IivF High-affinity branched-chain amino acid 2228837 2228112−2 726 transport ATP-binding protein TM.orf2011 braF ABC transporterATPase 2229627 2228830 −1 798 TM.orf2012 branched-chain amino acid ABCtransporter, 2231062 2229866 −3 1197 periplasmic branched-chain aminoacid- binding protein TM.orf2013 hyfR PAS modulated sigma54 specific2232828 2231296 −1 1533 transcriptional regulator, Fis family TM.orf2014besA putative esterase 2233914 2232955 −1 960 TM.orf2015 mdlC thiaminepyrophosphate binding domain- 2235657 2233963 −1 1695 containing proteinTM.orf2016 hmp oxidoreductase FAD/NAD(P)-binding subunit 2236949 2235744−2 1206 TM.orf2017 conserved hypothetical protein 2237212 2238534 1 1323TM.orf2018 wcaL glucosyltransferase 2238557 2239822 3 1266 TM.orf2019Glycosyl transferase, group 1 2239822 2241012 1 1191 TM.orf2020phosphoglycerate mutase 2241009 2241626 2 618 TM.orf2021 conservedhypothetical protein 2241641 2242849 3 1209 TM.orf2022 conservedhypothetical protein 2242856 2243626 3 771 TM.orf2023 hypotheticalprotein 2243807 2244343 3 537 TM.orf2024 gsiA putativemureinpeptideoligopeptide ABC 2244340 2246259 1 1920 transporterATP-binding protein TM.orf2025 agpA oligopeptide ABC transporter,periplasmic 2246256 2248238 2 1983 oligopeptide-binding proteinTM.orf2026 appB oligopeptide ABC transporter, permease 2248337 2249335 3999 protein TM.orf2027 appC putative oligopeptide ABC transporter,2249332 2250486 1 1155 permease protein TM.orf2028 CRP/FNR familytranscriptional regulator 2251329 2250565 −1 765 TM.orf2029 uppPundecaprenyl pyrophosphate phosphatase 2251593 2252426 2 834 TM.orf2030paaF enoyl-CoA hydratase/isomerase 2252736 2253500 2 765 TM.orf2031creatininase 2253493 2254263 1 771 TM.orf2032 luciferase family protein2254407 2255546 2 1140 TM.orf2033 enoyl-CoA hydratase/isomerase 22555752256363 1 789 TM.orf2034 AMP-dependent synthetase and ligase 22564912258272 3 1782 TM.orf2035 ribB 3,4-dihydroxy-2-butanone 4-phosphate2258291 2259421 3 1131 synthase TM.orf2036 ntaB nitrilotriacetatemonooxygenase component B 2259523 2260062 1 540 protein TM.orf2037 yjjLmajor facilitator family transporter 2260395 2261744 2 1350 TM.orf2038yeaM putative transcriptional regulator 2262725 2261847 −2 879TM.orf2039 AsnC-family transcriptional regulator 2262982 2263479 1 498TM.orf2040 dusA tRNA-dihydrouridine synthase A 2263678 2264670 1 993TM.orf2041 putative membrane protein 2264816 2265505 3 690 TM.orf2042hypothetical protein 2265502 2265876 1 375 TM.orf2043 conservedhypothetical protein 2266046 2267356 3 1311 TM.orf2044 extracellularligand-binding receptor 2268558 2267443 −1 1116 TM.orf2045 conservedhypothetical protein 2269045 2268692 −3 354 TM.orf2046 IivFbranched-chain amino acid transport system 2269784 2269074 −2 711ATP-binding protein TM.orf2047 IivG ABC transporter component 22706712269784 −3 888 TM.orf2048 braE inner-membrane translocator 22720162270691 −2 1326 TM.orf2049 braD inner-membrane translocator 22729302272016 −3 915 TM.orf2050 hypothetical protein 2273700 2273446 −1 255TM.orf2051 hemA 5-aminolevulinate synthase 2275060 2273834 −3 1227TM.orf2052 putative transcriptional regulator protein 2275884 2275354 −1531 TM.orf2053 ygaD Putative multidrug export ATP- 2278615 2275931 −32685 binding/permease protein ygaD TM.orf2054 estA Esterase estA 22789372281075 3 2139 TM.orf2055 conserved hypothetical protein 2282164 2281118−3 1047 TM.orf2056 bax conserved hypothetical protein 2283102 2282185 −1918 TM.orf2057 hypothetical protein 2283527 2283207 −2 321 TM.orf2058chvG sensor histidine kinase 2285247 2283745 −1 1503 TM.orf2059 chvItranscriptional regulatory protein ChvI 2286110 2285262 −2 849TM.orf2060 conserved hypothetical protein 2286552 2286094 −1 459TM.orf2061 hypothetical protein 2287144 2287668 1 525 TM.orf2062conserved hypothetical protein 2289037 2288207 −3 831 TM.orf2063ATP-dependent RNA helicase, DEAD 2291518 2289044 −3 2475 TM.orf2064 ligputative mRNA processing factor 2291671 2292774 1 1104 TM.orf2065 ligATP-dependent DNA ligase 2292779 2294395 3 1617 TM.orf2066 conservedhypothetical protein 2294475 2296736 2 2262 TM.orf2067 conservedhypothetical protein 2296920 2297339 2 420 TM.orf2068 Major facilitatorsuperfamily MFS_1 2297336 2298787 3 1452 TM.orf2069 lipoprotein 22988082299350 1 543 TM.orf2070 corA Mg2 transporter protein CorA familyprotein 2299473 2300498 2 1026 TM.orf2071 hypothetical protein 23006252301026 2 402 TM.orf2072 cirA addiction module killer protein 23010742303014 3 1941 TM.orf2073 polyferredoxin 2303035 2304465 1 1431TM.orf2074 conserved hypothetical protein 2305361 2304483 −2 879TM.orf2075 FAD dependent oxidoreductase 2305472 2306527 3 1056TM.orf2076 HD domain protein 2306674 2307246 1 573 TM.orf2077 conservedhypothetical protein 2311926 2307280 −1 4647 TM.orf2078 ytfM surfaceantigen 2313988 2311976 −3 2013 TM.orf2079 lipolytic protein G-D-S-Lfamily 2316039 2315095 −1 945 TM.orf2080 opgC protein, require forsuccinylation of 2317235 2316042 −2 1194 osmoregulated periplasmicglucans TM.orf2081 oxalate/formate antiporter 2317714 2318352 1 639TM.orf2082 ybfB putative MFS transporter 2318417 2318923 3 507TM.orf2083 hypothetical protein 2318977 2319186 1 210 TM.orf2084 paaJDcaF 2320431 2319220 −1 1212 TM.orf2085 paaH 3-hydroxybutyryl-CoAdehydrogenase 2322033 2320468 −1 1566 TM.orf2086 pcaR transcriptionalregulator 2322284 2323099 3 816 TM.orf2087 protein, possibly involved inaromatic 2323156 2323644 1 489 compounds catabolism TM.orf2088hypothetical protein 2323701 2323865 2 165 TM.orf2089 serA putativephosphoglycerate dehydrogenase 2324871 2323915 −1 957 (PGDH), serA-likeprotein TM.orf2090 phnE phosphonate ABC transporter, inner 23257752324969 −3 807 membrane subunit TM.orf2091 phnD binding proteincomponent of ABC 2326913 2325879 −2 1035 phosphonate transporterTM.orf2092 phnC Phosphonates ABC transporter, ATP-binding 23278342326980 −2 855 protein TM.orf2093 conserved hypothetical protein 23281742328887 2 714 TM.orf2094 yhaH Inner membrane protein yhaH 23289782329553 2 576 TM.orf2095 guanylate kinase 2330170 2329577 −3 594TM.orf2096 phnM phosphonate metabolism protein PhnM 2331288 2330143 −11146 TM.orf2097 phnL Phosphonates transport ATP-binding protein 23320342331285 −2 750 phnL TM.orf2098 phnK phosphonate C-P lyase system proteinPhnK 2332848 2332039 −1 810 TM.orf2099 phnJ enzyme of phosphonatemetabolism 2333783 2332845 −2 939 TM.orf2100 phnI phosphonate metabolism2334925 2333780 −3 1146 TM.orf2101 phnH phosphonate C-P lyase systemprotein PhnH 2335559 2334930 −2 630 TM.orf2102 phnG PhnG protein 23360742335565 −3 510 TM.orf2103 phosphonate operon transcriptional regulator2336204 2337016 3 813 TM.orf2104 ppi peptidyl-prolyl cis-transisomerase, cyclophilin 2337191 2337766 3 576 type TM.orf2105 conservedhypothetical protein 2337886 2338764 1 879 TM.orf2106 transcriptionregulator 2340272 2340514 3 243 TM.orf2107 plasmid stabilization systemprotein 2340523 2340837 1 315 TM.orf2108 ppi peptidyl-prolyl cis-transisomerase cyclophilin 2341092 2341571 2 480 type TM.orf2109 recQATP-dependent DNA helicase 2341831 2343489 1 1659 TM.orf2110 ylaBputative signaling membrane protein 2345029 2343524 −3 1506 TM.orf2111conserved hypothetical protein 2346179 2345247 −2 933 TM.orf2112 patatin2346754 2347893 1 1140 TM.orf2113 erfK conserved hypothetical protein2347890 2349365 2 1476 TM.orf2114 hypothetical protein 2349865 2349590−3 276 TM.orf2115 phenazine biosynthesis protein PhzF family 23510132350081 −1 933 TM.orf2116 ybfL transposase, is4 family 2352240 2351125−1 1116 TM.orf2117 yhfA redox protein 2352467 2352916 3 450 TM.orf2118ydhC drug resistance transporter, Bcr/CflA subfamily 2352999 2354153 21155 protein TM.orf2119 conserved hypothetical protein 2354473 2356797 12325 TM.orf2120 conserved hypothetical protein 2356794 2357492 2 699TM.orf2121 cbpA curved DNA-binding protein 2357752 2358750 1 999TM.orf2122 glutathione S-transferase 2359462 2358761 −3 702 TM.orf2123TrkA domain-containing protein 2361260 2359479 −2 1782 TM.orf2124 yfcGglutathione S-transferase-like protein 2361410 2362114 3 705 TM.orf2125quiP peptidase S45 penicillin amidase 2362290 2364647 2 2358 TM.orf2126siaT TRAP transporter, DctM-like membrane 2365995 2364673 −1 1323protein TM.orf2127 conserved hypothetical protein 2366552 2365992 −2 561TM.orf2128 dctP C4-dicarboxylate-binding periplasmic protein 23676552366552 −3 1104 TM.orf2129 ygaE transcriptional regulator 23685072367776 −3 732 TM.orf2130 cyclase family protein 2368754 2369530 3 777TM.orf2131 possible isomerase/decarboxylase 2369581 2370600 1 1020TM.orf2132 braF ABC transporter related protein 2370912 2371667 2 756TM.orf2133 IivF ABC-type branched-chain amino acid 2371681 2372397 1 717transport systems, ATPase component TM.orf2134 Extracellularligand-binding receptor 2372698 2373918 1 1221 TM.orf2135 branched chainamino acid ABC transporter 2373979 2374866 1 888 permease TM.orf2136IivM ABC-type branched-chain amino acid 2374871 2375854 3 984 transportsystem, permease component TM.orf2137 mcp4 Methyl-accepting chemotaxisprotein 2376055 2377737 1 1683 TM.orf2138 putative ABC branched-chainamino 2378066 2379283 3 1218 transporter, periplasmic binding proteinTM.orf2139 linocin_M18 bacteriocin protein 2380217 2379390 −2 828TM.orf2140 conserved hypothetical protein 2380648 2380265 −3 384TM.orf2141 Zinc transporter 2381604 2380786 −1 819 TM.orf2142 Permeasesof the major facilitator superfamily 2382992 2381766 −2 1227 TM.orf2143ydcO benzoate transporter subfamily 2384329 2383154 −3 1176 TM.orf2144mobB molybdopterin-guanine dinucleotide 2385012 2384446 −1 567biosynthesis MobB region TM.orf2145 nrtC nitrate transporter 23853442386798 3 1455 TM.orf2146 nrtB nitrate ABC transporter, inner membrane2386816 2387637 1 822 subunit TM.orf2147 nasD nitrate ABC transporter,ATPase subunits C 2387665 2388492 1 828 and D TM.orf2148 nasD F283498_4assimilatory nitrite reductase large 2388507 2390984 2 2478 subunitTM.orf2149 nasE Nitrite reductase (NAD(P)H) large subunit, 23909992391406 3 408 NirD TM.orf2150 nasA Anaerobic dehydrogenases, typically2391403 2393925 1 2523 selenocysteine-containing TM.orf2151 hypotheticalprotein 2393951 2394112 3 162 TM.orf2152 cysG siroheme synthase protein2394162 2395475 2 1314 TM.orf2153 trpD Glycosyl transferase, family 32395490 2396497 3 1008 TM.orf2154 evgS two-component sensor histidinekinase 2396494 2398014 1 1521 TM.orf2155 coxL carbon monoxidedehydrogenase large chain 2398195 2400567 1 2373 TM.orf2156 gltSsodium/glutamate symporter 2401753 2400554 −3 1200 TM.orf2157 yhhJ ABC-2type transporter 2402862 2401750 −1 1113 TM.orf2158 yhiH ABC transporterpermease ATP-binding 2405599 2402870 −3 2730 protein TM.orf2159 yhiIHlyD family secretion protein 2406633 2405602 −1 1032 TM.orf2160 envRTetR family transcriptional regulator 2406898 2407506 1 609 TM.orf2161conserved hypothetical protein 2407616 2407921 3 306 TM.orf2162conserved hypothetical protein 2408835 2407939 −1 897 TM.orf2163 yxnAshort chain dehydrogenase 2409110 2410153 3 1044 TM.orf2164 conservedhypothetical protein 2410172 2410669 3 498 TM.orf2165 conservedhypothetical protein 2411430 2410681 −1 750 TM.orf2166 molybdopterinbinding domain protein 2412483 2411680 −1 804 TM.orf2167 putativeNAD-dependent 2413505 2412480 −2 1026 epimerase/dehydratase TM.orf2168sugar fermentation stimulation protein A 2413667 2414458 3 792TM.orf2169 map methionine aminopeptidase, type I 2414609 2415403 3 795TM.orf2170 DNA repair proteins 2415435 2416274 2 840 TM.orf2171 intAintegrase family protein 2416608 2417708 2 1101 TM.orf2172 conservedhypothetical protein 2418398 2418892 3 495 TM.orf2173 conservedhypothetical protein 2419006 2419677 1 672 TM.orf2174 superfamily IDNA/RNA helicase 2419788 2423363 2 3576 TM.orf2175phosphatidylserine/phosphatidylglycerophosphate/ 2423360 2425150 3 1791cardiolipin synthase-like protein TM.orf2176 conserved hypotheticalprotein 2425981 2425283 −3 699 TM.orf2177 conserved hypothetical protein2426516 2425986 −2 531 TM.orf2178 glutaredoxin 2427986 2427276 −2 711TM.orf2179 Dps family DNA-binding stress response 2428909 2429463 1 555protein TM.orf2180 rhaS transcriptional regulator, AraC family 24302132429470 −1 744 TM.orf2181 yxaF transcriptional regulator, TetR family2430962 2430372 −2 591 TM.orf2182 ydeP HxlR family transcriptionalregulator 2431847 2431473 −2 375 TM.orf2183 ywbO DSBA oxidoreductase2431963 2432751 1 789 TM.orf2184 conserved hypothetical protein 24327382433883 3 1146 TM.orf2185 FAD-dependent pyridine nucleotide-disulphide2433896 2435191 3 1296 oxidoreductase TM.orf2186 conserved hypotheticalprotein 2436402 2436115 −1 288 TM.orf2187 trbI conjugation TrbI familyprotein 2437524 2436406 −1 1119 TM.orf2188 trbG P-type conjugativetransfer protein TrbG 2438321 2437593 −2 729 TM.orf2189 trbF conjugaltransfer protein 2439253 2438570 −3 684 TM.orf2190 trbL P-typeconjugative transfer protein TrbL 2440554 2439256 −1 1299 TM.orf2191P-type conjugative transfer protein TrbJ 2441724 2440960 −1 765TM.orf2192 trbE conjugal transfer ATPase TrbE 2444159 2441721 −2 2439TM.orf2193 type IV secretory pathway VirB3 family protein 24444362444164 −1 273 TM.orf2194 conjugal transfer protein TrbC 2444756 2444436−2 321 TM.orf2195 trbB conjugal transfer protein trbB 2445673 2444753 −3921 TM.orf2196 conserved hypothetical protein 2446792 2446364 −3 429TM.orf2197 traG TRAG protein 2448802 2446802 −3 2001 TM.orf2198conserved hypothetical protein 2450722 2448923 −3 1800 TM.orf2199 yjbJlytic transglycosylase, catalytic 2451818 2450907 −2 912 TM.orf2200 traFPeptidase S26, conserved region 2452339 2451821 −3 519 TM.orf2201conserved hypothetical protein 2452800 2452336 −1 465 TM.orf2202conserved hypothetical protein 2453042 2452797 −2 246 TM.orf2203putative partition protein 2453677 2453039 −3 639 TM.orf2204 replicationprotein A 2454747 2453674 −1 1074 TM.orf2205 conserved hypotheticalprotein 2455050 2454763 −1 288 TM.orf2206 conserved hypothetical protein2455516 2455157 −3 360 TM.orf2207 conserved hypothetical protein 24565002456216 −3 285 TM.orf2208 XRE family transcriptional regulator 24568422456994 1 153 TM.orf2209 SAM-dependent methyltransferase 2457058 24578911 834 TM.orf2210 conserved hypothetical protein 2458289 2457966 −2 324TM.orf2211 DNA polymerase III alpha subunit 2462233 2458967 −3 3267TM.orf2212 imuB conserved hypothetical protein 2463621 2462233 −1 1389TM.orf2213 imuA conserved hypothetical protein 2464411 2463659 −3 753TM.orf2214 hypothetical protein 2464755 2464922 2 168 TM.orf2215conserved hypothetical protein 2465463 2464963 −1 501 TM.orf2216conserved hypothetical protein 2465757 2465584 −1 174 TM.orf2217 yoaMconserved hypothetical protein 2466560 2465973 −2 588 TM.orf2218 Gene 61protein 2468052 2467114 −1 939 TM.orf2219 conserved hypothetical protein2469420 2468281 −1 1140 TM.orf2220 putative methylase/helicase 24737742469431 −3 4344 TM.orf2221 RC187 2474539 2473940 −3 600 TM.orf2222conserved hypothetical protein 2474999 2474625 −2 375 TM.orf2223conserved hypothetical protein 2475501 2475010 −1 492 TM.orf2224conserved hypothetical protein 2476137 2475571 −1 567 TM.orf2225 yubMParB domain protein nuclease 2478356 2476200 −2 2157 TM.orf2226conserved hypothetical protein 2479337 2478537 −2 801 TM.orf2227putative ardC antirestriction protein 2480466 2479507 −1 960 TM.orf2228conserved hypothetical protein 2480783 2481640 3 858 TM.orf2229conserved hypothetical protein 2483151 2481637 −1 1515 TM.orf2230 Domainof unknown function DUF1863 2483227 2483682 1 456 TM.orf2231 conservedhypothetical protein 2483721 2484341 2 621 TM.orf2232 conservedhypothetical protein 2484829 2484362 −3 468 TM.orf2233 conservedhypothetical protein 2485356 2484841 −1 516 TM.orf2234 copB copperresistance B precursor 2486148 2485456 −1 693 TM.orf2235 pcoAcopper-resistance protein, CopA family 2488406 2486616 −2 1791TM.orf2236 conserved hypothetical protein 2489421 2488522 −1 900TM.orf2237 Heavy metal transport/detoxification protein 2489652 2489437−1 216 TM.orf2238 heavy metal translocating P-type ATPase 24898152492364 1 2550 TM.orf2239 nrdH glutaredoxin 2492427 2492693 2 267TM.orf2240 ndoR oxidoreductase FAD-binding subunit 2492708 2493742 31035 TM.orf2241 transcriptional regulator, MerR family 2493769 2494266 1498 TM.orf2242 ydeG riboflavin synthase subunit alpha 2494328 2495533 31206 TM.orf2243 hypothetical protein 2495807 2495577 −2 231 TM.orf2244conserved hypothetical protein 2496107 2495841 −2 267 TM.orf2245 cationdiffusion facilitator family transporter 2497148 2496210 −2 939TM.orf2246 bepE hydrophobe/amphiphile efflux-1 (HAE1) family 25003392497169 −3 3171 protein TM.orf2247 acrA secretion protein HlyD 25015742500336 −1 1239 TM.orf2248 ynfL LysR family transcriptional regulator2501812 2502717 1 906 TM.orf2249 conserved hypothetical protein 25030762503306 3 231 TM.orf2250 DNA repair protein RadC 2503412 2503576 3 165TM.orf2251 cya2 adenylate cyclase protein 2504733 2503585 −1 1149TM.orf2252 purB adenylosuccinate lyase 2505040 2506338 1 1299 TM.orf2253hypothetical protein 2506873 2506412 −3 462 TM.orf2254Metallophosphoesterase 2507048 2507887 3 840 TM.orf2255 conservedhypothetical protein 2508288 2507971 −1 318 TM.orf2256enoyl-[acyl-carrier-protein] reductase (NADH) 2509244 2508462 −2 783TM.orf2257 purC phosphoribosylaminoimidazole- 2509598 2510368 3 771succinocarboxamide synthase TM.orf2258 yexAphosphoribosylformylglycinamidine synthase 2510365 2510610 1 246 proteinTM.orf2259 purQ phosphoribosylformylglycinamidine synthase I 25106482511385 3 738 TM.orf2260 purL Phosphoribosylformylglycinamidine (FGAM)2511463 2513316 1 1854 synthase, synthetase domain TM.orf2261 purLphosphoribosylformylglycinamidine synthase II 2513280 2513669 2 390TM.orf2262 BolA family protein 2513749 2513985 1 237 TM.orf2263glutaredoxin-like protein 2514053 2514406 3 354 TM.orf2264 conservedhypothetical protein 2514838 2514488 −3 351 TM.orf2265 PepSY-associatedTM helix domain protein 2516373 2514835 −1 1539 TM.orf2266 hypotheticalprotein 2516633 2516370 −2 264 TM.orf2267 fhuA TonB-dependentsiderophore receptor 2519107 2516627 −3 2481 TM.orf2268 fecR twocomponent sensor kinase 2520175 2519261 −3 915 TM.orf2269 sigma factor2520741 2520217 −1 525 TM.orf2270 short chain dehydrogenase 25215302520787 −1 744 TM.orf2271 mtaD 5-methylthioadenosine/S- 2521714 25231261 1413 adenosylhomocysteine deaminase TM.orf2272 metC cystathioninebeta-lyase 2524377 2523199 −1 1179 TM.orf2273 glpE rhodanese-likeprotein 2526621 2524378 −1 2244 TM.orf2274 pecT transcriptionalregulator, LysR family protein 2526749 2527594 3 846 TM.orf2275conserved hypothetical protein 2529128 2527611 −2 1518 TM.orf2276hypothetical protein 2529581 2529147 −2 435 TM.orf2277 conservedhypothetical protein 2530717 2529758 −3 960 TM.orf2278 conservedhypothetical protein 2531797 2531267 −3 531 TM.orf2279 fixN cbb3-typecytochrome c oxidase subunit I 2532412 2533872 1 1461 TM.orf2280 FixO1cytochrome C oxidase subunit 2533890 2534651 2 762 TM.orf2281hypothetical protein 2534648 2534815 3 168 TM.orf2282 petJ cytochrome-coxidase fixP chain 2534802 2535671 2 870 TM.orf2283 fixG cytochrome coxidase accessory protein CcoG 2535668 2537248 3 1581 TM.orf2284nitrogen fixation protein fixH 2537335 2537778 1 444 TM.orf2285hypothetical protein 2537883 2538539 2 657 TM.orf2286 conservedhypothetical protein 2538569 2539285 3 717 TM.orf2287 rpsD 30S ribosomalprotein S4 2540006 2539392 −2 615 TM.orf2288 hmuV ABC transporterrelated protein 2540874 2540083 −1 792 TM.orf2289 transport systempermease protein 2541866 2540871 −2 996 TM.orf2290 yvrC Fe3+-hydroxamateABC superfamily ATP 2542804 2541863 −3 942 binding cassette transporterperiplasmic family protein TM.orf2291 btuB TonB-dependent receptor2544963 2542804 −1 2160 TM.orf2292 Methyltransferase type 11 25458172545011 −2 807 TM.orf2293 pncA isochorismatase hydrolase 2547811 2546240−3 1572 TM.orf2294 alpha-isopropylmalate/homocitrate synthase 25495022547865 −1 1638 family transferase TM.orf2295 hypothetical protein2549747 2549610 −2 138 TM.orf2296 conserved hypothetical protein 25515062550802 −1 705 TM.orf2297 cysS Cysteinyl-tRNA synthetase 2553762 2552371−1 1392 TM.orf2298 glutamyl-tRNA synthetase 2555212 2553857 −3 1356TM.orf2299 nadE Glutamine-dependent NAD(+) synthetase 2556984 2555293 −11692 TM.orf2300 nicotinate phosphoribosyltransferase 2558129 2556999 −21131 TM.orf2301 phospho-2-dehydro-3-deoxyheptonate 2559694 2558300 −31395 aldolase 1 TM.orf2302 gor glutathione-disulfide reductase 25613962560047 −2 1350 TM.orf2303 gabD succinic semialdehyde dehydrogenase2562979 2561489 −3 1491 TM.orf2304 hypothetical protein 2563165 25635241 360 TM.orf2305 hypothetical protein 2564350 2564532 1 183 TM.orf2306FAH fumarylacetoacetase 2565929 2564673 −2 1257 TM.orf2307 ybiHtranscriptional regulator 2566637 2565945 −2 693 TM.orf2308 mtaDamidohydrolase 2568043 2566637 −3 1407 TM.orf2309 yiiZ DctP 25682482569267 3 1020 TM.orf2310 tripartite ATP-independent periplasmic 25693042569834 3 531 transporter DctQ TM.orf2311 TRAP dicarboxylatetransporter, DctM subunit 2569834 2571129 1 1296 TM.orf2312 cupin 2domain-containing protein 2571163 2571690 1 528 TM.orf2313 glmSglucosamine-fructose-6-phosphate 2573607 2571781 −1 1827aminotransferase TM.orf2314 conserved hypothetical protein 25754842574030 −2 1455 TM.orf2315 protein, phage-related 2578821 2575528 −13294 TM.orf2316 conserved hypothetical protein 2579651 2578818 −2 834TM.orf2317 conserved hypothetical protein 2580302 2579667 −2 636TM.orf2318 hypothetical protein 2580467 2580315 −2 153 TM.orf2319hypothetical protein 2581272 2580481 −1 792 TM.orf2320 hypotheticalprotein 2581716 2581288 −1 429 TM.orf2321 hypothetical protein 25821052581716 −2 390 TM.orf2322 conserved hypothetical protein 2582563 2582102−3 462 TM.orf2323 conserved hypothetical protein 2583563 2582586 −2 978TM.orf2324 hypothetical protein 2584181 2583597 −2 585 TM.orf2325conserved hypothetical protein 2585579 2584194 −2 1386 TM.orf2326putative phage terminase-like protein 2587024 2585618 −3 1407 TM.orf2327dinB conserved hypothetical protein 2587742 2587221 −2 522 TM.orf2328conserved hypothetical protein 2587970 2588917 3 948 TM.orf2329 SRY1threonine dehydratase 2590188 2588929 −1 1260 TM.orf2330 cvrAsodium/hydrogen exchanger 2590380 2592305 2 1926 TM.orf2331 conservedhypothetical protein 2592646 2592320 −3 327 TM.orf2332prevent-host-death protein 2593009 2592758 −3 252 TM.orf2333fumarylacetoacetat hydroxylase 2593778 2593083 −2 696 TM.orf2334glycolate reductase 2594922 2593930 −1 993 TM.orf2335 cysG sirohemesynthase 2595157 2595816 1 660 TM.orf2336 cysH phosphoadenosinephosphosulfate reductase 2595894 2596604 2 711 TM.orf2337 cysQ3′(2′),5′-bisphosphate nucleotidase 2596698 2597522 2 825 TM.orf2338hypothetical protein 2597519 2598121 3 603 TM.orf2339 soj cobyrinic acida,c-diamide synthase 2598752 2598126 −2 627 TM.orf2340 hypotheticalprotein 2598950 2599210 3 261 TM.orf2341 ywlC putative translationfactor 2599222 2600265 1 1044 TM.orf2342 pleC Signal transductionhistidine kinase 2600307 2601653 2 1347 TM.orf2343 conservedhypothetical protein 2602071 2601631 −1 441 TM.orf2344FAD/FMN-containing dehydrogenases 2602148 2603581 3 1434 TM.orf2345formiminoglutamase 2604410 2603598 −2 813 TM.orf2346 hutH histidineammonia-lyase 2605963 2604407 −3 1557 TM.orf2347 mtaDN-formimino-L-glutamate deiminase 2607327 2605960 −1 1368 TM.orf2348hutI imidazolonepropionase 2607424 2608689 1 1266 TM.orf2349 hutCtranscriptional regulator 2608686 2609420 2 735 TM.orf2350 type IV pilusassembly PilZ 2610874 2609423 −3 1452 TM.orf2351 cysM cysteine synthase2611161 2612144 2 984 TM.orf2352 ocd putative ornithine cyclodeaminase2612141 2613178 3 1038 TM.orf2353 type IV pilus assembly PilZ 26146432613186 −2 1458 TM.orf2354 yrhP Lysine exporter protein (LYSE/YGGA)2614803 2615441 2 639 TM.orf2355 acetoacetyl-CoA synthetase 26154852616360 1 876 TM.orf2356 mcpA methyl-accepting chemotaxis protein2618629 2616383 −3 2247 TM.orf2357 cpxA Sensor protein cpxA 26201462618797 −1 1350 TM.orf2358 mtrA Two-component system response regulator2620832 2620143 −2 690 TM.orf2359 macA macrolide efflux protein MacA2621091 2622275 2 1185 TM.orf2360 macB ABC transporter related protein2622280 2624235 1 1956 TM.orf2361 multidrug efflux outer membranelipoprotein 2624232 2625647 2 1416 TM.orf2362 prtA hemolysin-typecalcium-binding region 2625880 2627820 1 1941 TM.orf2363glyoxalase/bleomycin resistance 2628245 2627829 −2 417protein/dioxygenase TM.orf2364 acnR transcriptional regulator, TetRfamily 2628388 2628990 1 603 TM.orf2365 conserved hypothetical protein2629744 2628974 −3 771 TM.orf2366 fadA acetyl-CoA acetyltransferase2629890 2630273 2 384 TM.orf2367 conserved hypothetical protein 26307832630322 −2 462 TM.orf2368 conserved hypothetical protein 2631894 2630842−1 1053 TM.orf2369 conserved hypothetical protein 2632694 2631894 −2 801TM.orf2370 conserved hypothetical protein 2636857 2632691 −3 4167TM.orf2371 phage tape measure protein 2643896 2637249 −2 6648 TM.orf2372conserved hypothetical protein 2644634 2644269 −2 366 TM.orf2373conserved hypothetical protein 2645607 2644651 −1 957 TM.orf2374hypothetical protein 2646244 2645810 −3 435 TM.orf2375 conservedhypothetical protein 2646657 2646241 −1 417 TM.orf2376 hypotheticalprotein 2646902 2646669 −2 234 TM.orf2377 phage capsid protein 26478462646902 −3 945 TM.orf2378 hypothetical protein 2648255 2647863 −2 393TM.orf2379 conserved hypothetical protein 2649469 2648255 −3 1215TM.orf2380 phage virion morphogenesis protein 2650168 2649653 −3 516TM.orf2381 putative head morphogenesis protein SPP1 2651240 2650296 −2945 gp7 TM.orf2382 F, portal protein 2652801 2651527 −1 1275 TM.orf2383putative phage-related protein 2654573 2653014 −2 1560 TM.orf2384terminase small subunit 2655147 2654620 −1 528 TM.orf2385 hypotheticalprotein 2655493 2655170 −3 324 TM.orf2386 conserved hypothetical protein2655909 2655490 −1 420 TM.orf2387 hypothetical protein 2656112 2655894−2 219 TM.orf2388 hypothetical protein 2656566 2656105 −1 462 TM.orf2389conserved hypothetical protein 2657171 2656569 −2 603 TM.orf2390conserved hypothetical protein 2657702 2657205 −2 498 TM.orf2391hypothetical protein 2657922 2657707 −1 216 TM.orf2392 Mu-like prophageprotein GP16 2658512 2657919 −2 594 TM.orf2393 hypothetical protein2658720 2658505 −1 216 TM.orf2394 hypothetical protein 2658943 2658713−3 231 TM.orf2395 hypothetical protein 2659374 2658940 −1 435 TM.orf2396conserved hypothetical protein 2659622 2659371 −2 252 TM.orf2397hypothetical protein 2659759 2659619 −3 141 TM.orf2398 hypotheticalprotein 2660297 2660073 −2 225 TM.orf2399 hypothetical protein 26605222660313 −2 210 TM.orf2400 putative transcriptional regulator 26609292660519 −3 411 TM.orf2401 phage-related conserved putative protein2661563 2660922 −2 642 TM.orf2402 phage transposition protein B 26620652661565 −1 501 TM.orf2403 hypothetical protein 2662663 2662379 −3 285TM.orf2404 Transposase-like Mu 2664791 2662674 −2 2118 TM.orf2405putative cytosolic protein 2665269 2664838 −1 432 TM.orf2406hypothetical protein 2665798 2665917 1 120 TM.orf2407 XRE familyDNA-binding protein 2666075 2666419 3 345 TM.orf2408 fadA acetyl-CoAacetyltransferase 2666886 2667605 2 720 TM.orf2409ribonucleotide-diphosphate reductase subunit 2668675 2667572 −3 1104beta TM.orf2410 ribonucleotide-diphosphate reductase subunit 26701352668681 −1 1455 alpha TM.orf2411 NrdA protein 2670595 2670113 −3 483TM.orf2412 XRE family transcriptional regulator 2672385 2670961 −1 1425TM.orf2413 2-methylcitrate dehydratase 2672553 2674067 2 1515 TM.orf2414prpC 2-methylcitrate synthase/citrate synthase II 2674090 2675289 1 1200TM.orf2415 prpB methylisocitrate lyase 2675307 2676224 2 918 TM.orf2416metE 5-methyltetrahydropteroyltriglutamate-- 2676226 2678535 1 2310homocysteine S-methyltransferase TM.orf2417 oatA acyltransferase 32678614 2679765 1 1152 TM.orf2418 asnO asparagine synthase 26817252679773 −3 1953 TM.orf2419 conserved hypothetical protein 26819712681738 −3 234 TM.orf2420 hypothetical protein 2682220 2682065 −3 156TM.orf2421 menE long-chain-fatty-acid-CoA ligase 2684066 2682306 −2 1761TM.orf2422 enoyl-CoA hydratase 2685023 2684121 −2 903 TM.orf2423 TetRfamily transcriptional regulator 2685200 2685826 3 627 TM.orf2424 osmCOsmC-like protein 2686269 2685838 −1 432 TM.orf2425 conservedhypothetical protein 2686422 2686754 2 333 TM.orf2426 membrane protein2686744 2687520 1 777 TM.orf2427 ydfF transcriptional regulator, ArsRfamily protein 2687572 2688282 1 711 TM.orf2428 putative lantibioticmodifying enzyme 2688293 2691013 3 2721 TM.orf2429 conservedhypothetical protein 2691119 2691400 3 282 TM.orf2430 yjcE Na+/H+antiporter 2693071 2691434 −3 1638 TM.orf2431 pobA 4-hydroxybenzoate3-monooxygenase 2694396 2693212 −1 1185 TM.orf2432 putative3-carboxy-cis,cis-muconate 2695588 2694389 −3 1200 cycloisomerase(3-carboxymuconate lactonizing enzyme) TM.orf2433 pcaG Protocatechuate3,4-dioxygenase, alpha 2696165 2695581 −2 585 subunit(PcaG) TM.orf2434pcaH protocatechuate 3,4-dioxygenase, beta 2696874 2696170 −1 705subunit TM.orf2435 pcaC 4-carboxymuconolactone decarboxylase 26972842696871 −2 414 TM.orf2436 pcaQ LysR family transcriptional regulator2697367 2698353 1 987 TM.orf2437 siaT TRAP transporter, DctM subunitsubfamily 2699656 2698319 −3 1338 TM.orf2438 conserved hypotheticalprotein 2700131 2699661 −2 471 TM.orf2439 siaP Bacterial extracellularsolute-binding protein, 2701242 2700211 −1 1032 family 7 TM.orf2440conserved hypothetical protein 2701520 2702683 3 1164 TM.orf2441conserved hypothetical protein 2702696 2703175 3 480 TM.orf2442conserved hypothetical protein 2709283 2709717 1 435 TM.orf2443 putativeacetyltransferase protein 2710251 2709745 −1 507 TM.orf2444 ygeXdiaminopropionate ammonia-lyase 2711459 2710248 −2 1212 TM.orf2445transcriptional regulator, AsnC family 2711571 2712083 2 513 TM.orf2446conserved hypothetical protein 2713314 2712058 −1 1257 TM.orf2447 YciIfamily protein 2713649 2713362 −2 288 TM.orf2448 thyX thymidylatesynthase (FAD) 2714649 2713711 −1 939 TM.orf2449 conserved hypotheticalprotein 2715206 2715721 3 516 TM.orf2450 ywhH YbaK/prolyl-tRNAsynthetase associated 2715728 2716246 3 519 region TM.orf2451 conservedhypothetical protein 2716311 2716613 2 303 TM.orf2452 ETC complex Isubunit region 2716907 2717200 3 294 TM.orf2453 conserved hypotheticalprotein 2717483 2718088 3 606 TM.orf2454 visC conserved hypotheticalprotein 2719289 2718096 −2 1194 TM.orf2455 putative transmembraneprotein 2720401 2719370 −3 1032 TM.orf2456 pecT LysR familytranscriptional regulator 2720510 2721382 3 873 TM.orf2457 conservedhypothetical protein 2721745 2721386 −3 360 TM.orf2458 conservedhypothetical protein 2722102 2721809 −3 294 TM.orf2459 nahRtranscriptional regulator, LysR family 2723159 2722215 −2 945 TM.orf2460yebQ major facilitator superfamily MFS_1 2723305 2724750 1 1446TM.orf2461 conserved hypothetical protein 2725624 2724725 −3 900TM.orf2462 ycaN transcriptional regulator, LysR family 2726568 2725621−1 948 TM.orf2463 yajO aldo/keto reductase 2726686 2727714 1 1029TM.orf2464 conserved hypothetical protein 2728262 2727711 −2 552TM.orf2465 gluconolactonase precursor 2728368 2729294 2 927 TM.orf2466conserved hypothetical protein 2729352 2729954 2 603 TM.orf2467 yhcXhydrolase, carbon-nitrogen family protein 2729951 2730880 3 930TM.orf2468 hypothetical protein 2731163 2730882 −2 282 TM.orf2469hypothetical protein 2731683 2731336 −1 348 TM.orf2470 conservedhypothetical protein 2732253 2731702 −1 552 TM.orf2471 putative ABCtransporter 2732676 2733464 2 789 TM.orf2472 ABC transporter permeaseprotein 2733461 2734240 3 780 TM.orf2473 putative sulfonate/nitratetransport system 2734283 2735254 3 972 substrate-binding proteinTM.orf2474 cation antiporter 2735608 2736081 1 474 TM.orf2475 multipleresistance and pH regulation protein F 2736081 2736404 2 324 TM.orf2476monovalent cation/proton antiporter, 2736451 2736756 1 306 MnhG/PhaGsubunit TM.orf2477 Na+/H+ antiporter MnhB subunit-related 27367532737361 2 609 protein TM.orf2478 putative monovalent cation/H+antiporter 2737351 2737776 1 426 subunit B TM.orf2479 phaCNADH-ubiquinone oxidoreductase chain 4L 2737778 2738347 3 570 TM.orf2480nuoN NADH dehydrogenase (quinone) 2738344 2739831 1 1488 TM.orf2481 nuoNNADH dehydrogenase (quinone) 2739836 2741353 3 1518 TM.orf2482hypothetical protein 2741350 2741589 1 240 TM.orf2483 nuoN putativemonovalent cation/H+ antiporter 2741582 2743303 3 1722 subunit DTM.orf2484 yjaB acetyltransferase 2743501 2744055 1 555 TM.orf2485conserved hypothetical protein 2744124 2745497 2 1374 TM.orf2486transmembrane protein 2745879 2745511 −1 369 TM.orf2487 iron-regulatedprotein 2746035 2746958 2 924 TM.orf2488 ykvO short chain dehydrogenase2747854 2747102 −3 753 TM.orf2489 glutathione S-transferasedomain-containing 2748690 2748100 −1 591 protein TM.orf2490 yqhCtranscriptional regulator protein 2748951 2749880 2 930 TM.orf2491 TetRfamily transcriptional regulator 2750587 2749901 −3 687 TM.orf2492hypothetical protein 2750599 2750718 1 120 TM.orf2493 class I peptidechain release factor 2751175 2750732 −3 444 TM.orf2494 mcp4Methyl-accepting chemotaxis protein 2753444 2751255 −2 2190 TM.orf2495dcrA methyl-accepting chemotaxis protein 2753759 2755423 3 1665TM.orf2496 htpX peptidase M48 Ste24p 2756252 2755389 −2 864 TM.orf2497hypothetical protein 2757400 2756330 −3 1071 TM.orf2498 ahpCalkylhydroperoxide reductase C 2757718 2758257 1 540 TM.orf2499 ahpDAhpD, alkyl hydroperoxide reductase D 2758548 2759081 2 534 TM.orf2500asnB asparagine synthase (glutamine-hydrolyzing) 2759614 2761425 1 1812TM.orf2501 cphA GNAT-family acetyltransferase TIGR03103 2761433 27632923 1860 TM.orf2502 yhfE peptidase M42 family hydrolase 2763289 2764512 11224 TM.orf2503 cysB cysteine synthase 2764722 2765726 2 1005 TM.orf2504Alanyl-tRNA editing protein alaX-M 2765778 2766548 2 771 TM.orf2505alkyl hydroperoxide reductase/Thiol specific 2767763 2766549 −2 1215antioxidant/Mal allergen TM.orf2506 MPST rhodanese-relatedsulfurtransferase 2768043 2768897 2 855 TM.orf2507 putativeacetyltransferase 2769196 2769819 1 624 TM.orf2508 ooxABFD/(2Fe—2S)-binding domain-containing 2769816 2771240 2 1425 proteinTM.orf2509 hypothetical protein 2771237 2772184 3 948 TM.orf2510alpha/beta hydrolase fold 2773161 2772196 −1 966 TM.orf2511 aapP ABCtransporter component 2774099 2773287 −2 813 TM.orf2512 aapM generalL-amino acid transport system 2775219 2774116 −1 1104 permease proteinTM.orf2513 yhdX putative amino acid ABC transporter 2776429 2775224 −31206 permease protein TM.orf2514 aapJ general L-amino acid transportsystem 2777638 2776607 −3 1032 substrate-binding protein TM.orf2515 metCcystathionine beta-lyase 2777991 2779223 2 1233 TM.orf2516 L-sorbosonedehydrogenase 2779267 2780544 1 1278 TM.orf2517 hypothetical protein2780657 2782402 3 1746 TM.orf2518 luxO two-component system responseregulator 2783883 2782414 −1 1470 LuxO TM.orf2519 FOG: TPR repeat, SEL1subfamily 2784735 2784028 −1 708 TM.orf2520 O-antigen polymerase 27860302784870 −2 1161 TM.orf2521 hypothetical protein 2787184 2786264 −3 921TM.orf2522 putative DNA helicase related protein 2790071 2787465 −2 2607TM.orf2523 putative DNA helicase related protein 2793105 2790073 −1 3033TM.orf2524 oxyR transcriptional regulator, LysR family 2793327 2794232 2906 TM.orf2525 yagI putative transcriptional regulator 2795001 2794255−1 747 TM.orf2526 TRAP dicarboxylate transporter, DctM subunit 27963432795048 −3 1296 TM.orf2527 Tripartite ATP-independent periplasmic2796936 2796388 −1 549 transporter DctQ component TM.orf2528 yiiZ TRAPdicarboxylate transporter- DctP subunit 2797997 2796951 −2 1047TM.orf2529 Enoyl-CoA hydratase/isomerase 2798877 2798095 −1 783TM.orf2530 paaG enoyl-CoA hydratase/isomerase 2799231 2800055 2 825TM.orf2531 L-carnitine dehydratase 2800069 2800272 1 204 TM.orf2532 yfdEL-carnitine dehydratase/bile acid-inducible 2800269 2801213 2 945protein F TM.orf2533 ydcS extracellular solute-binding protein family 12801396 2802571 3 1176 TM.orf2534 ydcT ABC transporter related protein2802653 2803717 3 1065 TM.orf2535 ydcU binding-protein-dependenttransport systems 2803714 2804667 1 954 inner membrane componentTM.orf2536 ydcV binding-protein-dependent transport systems 28046572805469 3 813 inner membrane component TM.orf2537 Aminobutyraldehydedehydrogenase 2805481 2806926 1 1446 TM.orf2538 short chaindehydrogenase 2807565 2806969 −1 597 TM.orf2539 yusZ short chaindehydrogenase 2807641 2808489 1 849 TM.orf2540 tuaC glycosyltransferase, group 1 family protein 2809650 2808493 −1 1158 TM.orf2541glmU UDP-N-acetylglucosamine pyrophosphorylase 2809869 2811233 2 1365TM.orf2542 amino acid carrier protein 2812699 2811248 −3 1452 TM.orf2543auxin efflux carrier 2812835 2813761 3 927 TM.orf2544 yvfR ABCtransporter ATP-binding protein 2813832 2814833 2 1002 TM.orf2545 ABC-2type transporter 2814833 2815645 3 813 TM.orf2546 pyrB Aspartatecarbamoyltransferase 2815807 2816742 1 936 TM.orf2547 pyrCdihydroorotase-like protein 2816827 2818170 1 1344 TM.orf2548 plsYGlycerol-3-phosphate acyltransferase 2818222 2818857 1 636 TM.orf2549ppsD AMP-dependent synthetase and ligase 2821610 2818842 −2 2769TM.orf2550 conserved hypothetical protein 2822581 2821583 −3 999TM.orf2551 smf Predicted Rossmann fold nucleotide-binding 28227782823923 2 1146 protein involved in DNA uptake TM.orf2552 topA DNAtopoisomerase I 2824023 2826851 2 2829 TM.orf2553 rnr RNAse R 28270442829389 2 2346 TM.orf2554 garL putative HpcH/HpaI aldolase familyprotein 2829389 2830159 3 771 TM.orf2555 bcr Bcr/CflA subfamily drugresistance transporter 2830184 2831434 3 1251 TM.orf2556 fprOxidoreductase FAD-binding domain protein 2831561 2832334 3 774TM.orf2557 hypothetical protein 2832962 2832366 −2 597 TM.orf2558 rpmGRibosomal protein L33 2833278 2833445 2 168 TM.orf2559 pleD responseregulator With diguanylate cyclase 2834972 2833596 −2 1377 (GGDEF)domain TM.orf2560 divK response regulator receiver protein 28353442834979 −2 366 TM.orf2561 mak putative kinase (mak-like)/transcriptional2835614 2836603 3 990 regulator, actin-like ATPase domain (NagC/XylR(ROK) familiy) TM.orf2562 ImpB/MucB/SamB family 2836817 2837962 3 1146TM.orf2563 Putative esterase 2838395 2837967 −2 429 TM.orf2564 conservedhypothetical protein 2839379 2838450 −2 930 TM.orf2565 CPA hydrolase,carbon-nitrogen family 2840387 2839506 −2 882 TM.orf2566 membraneprotein involved in aromatic 2840714 2842075 3 1362 hydrocarbondegradation TM.orf2567 aguA agmatine deiminase 2843195 2842119 −2 1077TM.orf2568 ackA acetate kinase 2844673 2843495 −3 1179 TM.orf2569 ptabifunctional enoyl-CoA hydratase/phosphate 2846073 2844670 −1 1404acetyltransferase TM.orf2570 ldh leucine dehydrogenase 2847277 2846210−3 1068 TM.orf2571 yybA transcriptional regulator 2848082 2847513 −2 570TM.orf2572 sodium/sulphate symporter 2849593 2848193 −3 1401 TM.orf25733-methylcrotonoyl-CoA carboxylase subunit 2852581 2849621 −3 2961 alphaTM.orf2574 paaF enoyl-CoA hydratase/isomerase 2853442 2852645 −3 798TM.orf2575 carboxyltransferase subunit of acetyl-CoA 2855110 2853503 −31608 carboxylase TM.orf2576 doxJ DSBA oxidoreductase 2855841 2855239 −1603 TM.orf2577 mmgC acyl-CoA dehydrogenase 2857054 2855921 −3 1134TM.orf2578 acetyl-CoA acetyltransferase 2858438 2857245 −2 1194TM.orf2579 IVD2 isovaleryl-CoA dehydrogenase 2859703 2858534 −3 1170TM.orf2580 thioesterase superfamily protein 2860250 2859762 −2 489TM.orf2581 oxyR LysR family transcriptional regulator 2860607 2861512 3906 TM.orf2582 conserved hypothetical protein 2861863 2861534 −3 330TM.orf2583 AraC family transcriptional regulator 2862942 2861932 −1 1011TM.orf2584 ThiJ/PfpI domain protein 2863077 2863775 2 699 TM.orf2585transporter 2863873 2865057 1 1185 TM.orf2586 fecI sigma-24 (FecI)2865141 2865656 2 516 TM.orf2587 fecR two component sensor histidinekinase 2865745 2866692 1 948 FecR/PupR TM.orf2588 bhuA TonB-dependentreceptor 2866810 2869065 1 2256 TM.orf2589 conserved hypotheticalprotein 2869604 2869125 −2 480 TM.orf2590 exoZ acyltransferase 3 28697342870780 2 1047 TM.orf2591 divalent cation transporter 2872171 2870762 −31410 TM.orf2592 maeB malic enzyme 2874567 2872258 −1 2310 TM.orf2593 NpBlipoate-protein ligase B 2874843 2875538 2 696 TM.orf2594 bioY putativebiotin transporter bioY 2876104 2875556 −3 549 TM.orf2595 conservedhypothetical protein 2876700 2876224 −1 477 TM.orf2596 acyPacylphosphatase 2877104 2876739 −2 366 TM.orf2597 DLD2 FAD linkedoxidase domain protein 2878610 2877171 −2 1440 TM.orf2598 Pccapropionyl-CoA carboxylase alpha chain 2880804 2878822 −1 1983 TM.orf2599hypothetical protein 2881093 2880827 −3 267 TM.orf2600 PCCBpropionyl-CoA carboxylase beta chain 2882666 2881134 −2 1533 TM.orf2601ATP synthase mitochondrial F1 complex 2883679 2882942 −3 738 assemblyfactor 2 TM.orf2602 hydrolase, haloacid dehalogenase-like family 28843922883676 −1 717 protein TM.orf2603 rluC ribosomal large subunitpseudouridine 2885369 2884389 −2 981 synthase C TM.orf2604 crcB crcBprotein 2885761 2885366 −3 396 TM.orf2605 rarA recombination factorprotein RarA 2887095 2885758 −1 1338 TM.orf2606 degP periplasmic serineprotease, Do 2888628 2887156 −1 1473 TM.orf2607 rplQ 50S ribosomalprotein L17 2889227 2888808 −2 420 TM.orf2608 rpoA DNA-directed RNApolymerase subunit alpha 2890401 2889385 −1 1017 TM.orf2609 rpsKribosomal protein S11 2890992 2890600 −1 393 TM.orf2610 rpsM ribosomalprotein S13 2891370 2891002 −1 369 TM.orf2611 adk adenylate kinase2892128 2891475 −2 654 TM.orf2612 secY preprotein translocase, SecYsubunit 2893468 2892125 −3 1344 TM.orf2613 rplO ribosomal protein L152894180 2893698 −2 483 TM.orf2614 rpmD 50S ribosomal protein L30 28944312894237 −3 195 TM.orf2615 rpsE small subunit ribosomal protein S52895000 2894437 −1 564 TM.orf2616 rplR ribosomal protein L18 28953732895014 −3 360 TM.orf2617 rplF large subunit ribosomal protein L62895921 2895388 −1 534 TM.orf2618 rpsH 30S ribosomal protein S8 28963312895933 −2 399 TM.orf2619 rpsN small subunit ribosomal protein S142896653 2896348 −1 306 TM.orf2620 rplE 50S ribosomal protein L5 28972142896675 −1 540 TM.orf2621 rplX ribosomal protein L24 2897549 2897232 −2318 TM.orf2622 rplN large subunit ribosomal protein L14 2897917 2897549−3 369 TM.orf2623 rpsQ ribosomal protein S17 2898186 2897950 −1 237TM.orf2624 rpmC 50S ribosomal protein L29 2898394 2898191 −3 204TM.orf2625 rplP 50S ribosomal protein L16 2898818 2898399 −2 420TM.orf2626 rpsC 30S ribosomal protein S3 2899534 2898857 −3 678TM.orf2627 rplV ribosomal protein L22 2899914 2899534 −1 381 TM.orf2628rpsS small subunit ribosomal protein S19 2900195 2899917 −2 279TM.orf2629 rplB Ribosomal protein L2 2901038 2900214 −2 825 TM.orf2630rplW Ribosomal protein L23 2901336 2901043 −1 294 TM.orf2631 rplD 50Sribosomal protein L4P 2901956 2901333 −2 624 TM.orf2632 rplC ribosomalprotein L3 2902644 2901967 −1 678 TM.orf2633 rpsJ Ribosomal protein S102903003 2902692 −2 312 TM.orf2634 tuf1 Translation elongation factor Tu2904382 2903192 −3 1191 TM.orf2635 fusA elongation factor Tu 29065182904437 −3 2082 TM.orf2636 rpsG 30S ribosomal protein S7 2907033 2906563−1 471 TM.orf2637 rpsL 30S ribosomal protein S12 2907431 2907060 −2 372TM.orf2638 rpoC DNA-directed RNA polymerase subunit beta′ 29121792907944 −3 4236 TM.orf2639 rpoB DNA-directed RNA polymerase subunit beta2916387 2912299 −1 4089 TM.orf2640 hypothetical protein 2916691 2916506−3 186 TM.orf2641 rplL large subunit ribosomal protein L7/L12 29175012917124 −3 378 TM.orf2642 rplJ 50S ribosomal protein L10 2918161 2917643−3 519 TM.orf2643 rplA ribosomal protein L1 2919219 2918500 −1 720TM.orf2644 rplK 50S ribosomal protein L11 2919652 2919224 −3 429TM.orf2645 nusG transcription antitermination protein NusG 29203742919841 −1 534 TM.orf2646 preprotein translocase subunit SecE 29205932920396 −1 198 TM.orf2647 tuf1 Translation elongation factor Tu 29224592921305 −1 1155 TM.orf2648 rlmB 23S rRNA (guanosine-2′-O-)- 29230552923909 3 855 methyltransferase TM.orf2649 mtrB two-component sensorhistidine kinase 2925366 2923870 −1 1497 TM.orf2650 cusR Responseregulator consisting of a CheY-like 2926069 2925353 −3 717 receiverdomain and a winged-helix DNA- binding domain TM.orf2651 degP putativeserine protease do-like 2927881 2926325 −3 1557 TM.orf2652 biotinsulfoxide reductase 2928767 2931172 3 2406 TM.orf2653 atm1 Iron-sulfurclusters transporter 2931181 2933037 1 1857 TM.orf2654 hypotheticalprotein 2933034 2933621 2 588 TM.orf2655 hypothetical protein 29338082933984 2 177 TM.orf2656 yyaM transporter yyaM 2934182 2935126 3 945TM.orf2657 CRY2 Deoxyribodipyrimidine photolyase 2935239 2936708 2 1470TM.orf2658 amine oxidase 2936714 2938120 3 1407 TM.orf2659 DUF1365domain containing protein 2938216 2938983 1 768 TM.orf2660Cyclopropane-fatty-acyl-phospholipid synthase 2938980 2940230 2 1251TM.orf2661 Short-chain dehydrogenase/reductase SDR 2940220 2941020 1 801TM.orf2662 conserved hypothetical protein 2941480 2941040 −3 441TM.orf2663 deoxyribodipyrimidine photolyase-related 2943093 2941477 −11617 protein TM.orf2664 Dehydrogenases with different specificities2943321 2943100 −1 222 (related to short-chain alcohol dehydrogenases)TM.orf2665 silC RND efflux system outer membrane 2945018 2943492 −2 1527lipoprotein TM.orf2666 bepE hydrophobe/amphiphile efflux-1 (HAE1) family2948190 2945038 −1 3153 protein TM.orf2667 bepD multidrug-efflux systemsecretion protein 2949383 2948187 −2 1197 TM.orf2668 bepR transcriptionregulator protein 2949681 2950376 2 696 TM.orf2669 mutator insertionsequence transposase 2950603 2950968 1 366 protein TM.orf2670 conservedhypothetical protein 2951085 2952566 2 1482 TM.orf2671 conservedhypothetical protein 2952556 2954118 1 1563 TM.orf2672 Dehydrogenase2956176 2954548 −1 1629 TM.orf2673 DNA polymerase bacteriophage-type2956396 2957304 1 909 TM.orf2674 slt Soluble lytic mureintransglycosylase and 2957490 2959313 2 1824 related regulatory proteinTM.orf2675 exbB tonB-system energizer ExbB 2959543 2960499 1 957TM.orf2676 exbD biopolymer transport protein 2960504 2960962 3 459TM.orf2677 tonB TonB family protein 2960959 2961804 1 846 TM.orf2678moaB molybdenum cofactor biosynthesis protein B 2961881 2962420 3 540TM.orf2679 radical SAM domain protein 2962496 2963704 3 1209 TM.orf2680arnT Undecaprenyl phosphate-alpha-4-amino-4- 2965367 2963712 −2 1656deoxy-L-arabinose arabinosyl transferase TM.orf2681 lpxBLipid-A-disaccharide synthase 2965708 2965364 −3 345 TM.orf2682 arnCglycosyl transferase family 2 2966607 2965705 −1 903 TM.orf2683 qseCperiplasmic sensor signal transduction 2968219 2966765 −3 1455 histidinekinase TM.orf2684 mprA response regulator 2968910 2968206 −2 705TM.orf2685 nudC MutT/NUDIX family protein 2969908 2968943 −3 966TM.orf2686 dnaX DNA polymerase III subunit gamma/tau 2970289 2972370 12082 TM.orf2687 transcriptional regulator 2972469 2972792 2 324TM.orf2688 AraC family transcriptional regulator 2972809 2973615 1 807TM.orf2689 glyoxalase/bleomycin resistance 2973643 2974044 1 402protein/dioxygenase TM.orf2690 cca polyA polymerase related protein2974125 2975264 2 1140 TM.orf2691 Ferritin, Dps family protein 29754452975948 2 504 TM.orf2692 HIT family protein 2976492 2975956 −1 537TM.orf2693 recR Recombinational DNA repair protein (RecF 2976645 29772442 600 pathway) TM.orf2694 UDP-glucose: sterol glucosyltransferase2977261 2978571 1 1311 TM.orf2695 putative signal peptide 29797042978574 −2 1131 TM.orf2696 hypothetical protein 2981781 2980027 −1 1755TM.orf2697 rmuC DNA recombination protein rmuC homolog 2983064 2981907−2 1158 TM.orf2698 def N-formylmethionyl-tRNA deformylase 29832642983818 1 555 TM.orf2699 fmt methionyl-tRNA formyltransferase fmt2983861 2984829 1 969 TM.orf2700 PepSY-associated TM helix 29870442985575 −3 1470 TM.orf2701 fhuA ferrichrome-iron receptor 29895232987049 −2 2475 TM.orf2702 fecR putative FecR 2990587 2989607 −3 981TM.orf2703 fecI sigma factor 2991125 2990580 −2 546 TM.orf2704hypothetical protein 2991823 2991218 −3 606 TM.orf2705 dapEsuccinyl-diaminopimelate desuccinylase 2993001 2991820 −1 1182TM.orf2706 dapD Tetrahydrodipicolinate N-succinyltransferase 29939182993052 −2 867 TM.orf2707 putative hydrolase 2994760 2993993 −3 768TM.orf2708 yhjK signal transduction protein 2994917 2996593 3 1677TM.orf2709 dcrA chemotaxis sensory transducer 2996892 2998247 2 1356TM.orf2710 argB acetylglutamate kinase 2999261 2998344 −2 918 TM.orf2711engB ribosome biogenesis GTP-binding protein 3000104 2999439 −2 666 YsxCTM.orf2712 oxaA 60 kDa inner membrane insertion protein 3001983 3000277−1 1707 TM.orf2713 conserved hypothetical protein 3002392 3001976 −3 417TM.orf2714 rnpA Bacterial ribonuclease P protein 3002886 3002389 −1 498TM.orf2715 rpmH 50S ribosomal protein L34 3003070 3002936 −3 135TM.orf2716 hypothetical protein 3003765 3003271 −1 495 TM.orf2717 ctpFATPase, E1-E2 type 3003941 3006679 3 2739 TM.orf2718 ydjX mercuricreductase 3006788 3007558 3 771 TM.orf2719 merA pyridinenucleotide-disulphide oxidoreductase 3007555 3009015 1 1461 dimerisationregion TM.orf2720 yycG periplasmic sensor signal transduction 30090343010491 1 1458 histidine kinase TM.orf2721 mdmC O-methyltransferase mdmC3011203 3010514 −3 690 TM.orf2722 ygiF conserved hypothetical protein3012925 3011234 −3 1692 TM.orf2723 gsiA ABC transporter, nucleotidebinding/ATPase 3013258 3015117 1 1860 protein TM.orf2724 gsiBextracellular solute-binding protein 3015235 3016773 1 1539 TM.orf2725gsiC binding-protein-dependent transport systems 3017180 3018103 3 924inner membrane component TM.orf2726 appC ABC transporter, membranespanning protein 3018116 3018961 3 846 (oligopeptide) TM.orf2727conserved hypothetical protein 3018999 3020756 2 1758 TM.orf2728 ABCtransporter related protein 3021633 3020782 −1 852 TM.orf2729 cmpBsulfate ester transporter permease protein 3022382 3021633 −2 750TM.orf2730 ssuC sulfate ester transport system permease 3023287 3022439−3 849 protein TM.orf2731 ssuA sulfate ester transport system substrate-3024407 3023304 −2 1104 binding protein TM.orf2732 conservedhypothetical protein 3025963 3024413 −3 1551 TM.orf2733 gcvA LysR familytranscriptional regulator 3026142 3027077 2 936 TM.orf2734 iron ABCtransporter substrate binding protein 3027174 3028073 2 900 TM.orf2735yfeB ABC transporter related protein 3028094 3029008 3 915 TM.orf2736yfeC Chelated iron transport system membrane 3029005 3029865 1 861protein yfeC TM.orf2737 yfeD Chelated iron transport system membrane3029862 3030698 2 837 protein yfeD TM.orf2738 narP DNA-binding responseregulator 3030785 3031390 3 606 TM.orf2739 envZ signal transductionhistidine kinase 3031393 3033165 1 1773 TM.orf2740 hypothetical protein3033317 3034462 3 1146 TM.orf2741 conserved hypothetical protein 30347063034455 −2 252 TM.orf2742 conserved hypothetical protein 3035290 3034970−3 321 TM.orf2743 lysS lysyl-tRNA synthetase, class I 3037119 3035458 −11662 TM.orf2744 yoaA ATP-dependent DNA helicase 3037259 3040222 3 2964TM.orf2745 conserved hypothetical protein 3040305 3040676 2 372TM.orf2746 rnhB Ribonuclease HII 3040806 3041465 2 660 TM.orf2747 ccrMmodification methylase 3041462 3042709 3 1248 TM.orf2748Tetratricopeptide repeat protein 3044104 3042713 −3 1392 TM.orf2749 yfhQA/G-specific adenine glycosylase 3045424 3044249 −3 1176 TM.orf2750 gcvALysR family transcriptional regulator 3046455 3045505 −1 951 TM.orf2751hypothetical protein 3046612 3046824 1 213 TM.orf2752 yddQisochorismatase family protein 3046859 3047482 3 624 TM.orf2753YCII-related 3047586 3048008 2 423 TM.orf2754 RNA polymerase sigma-70factor, ECF family 3047989 3049269 1 1281 protein TM.orf2755Zn-ribbon-containing, possibly RNA-binding 3049341 3049919 2 579 proteinand truncated derivatives TM.orf2756 DsbA oxidoreductase 3050099 30507973 699 TM.orf2757 smc chromosome segregation ATPase 3050825 3055081 34257 TM.orf2758 cobO cob(I)alamin adenosyltransferase 3056281 3055619 −3663 TM.orf2759 cobN cobaltochelatase 3060072 3056278 −1 3795 TM.orf2760cobW cobalamin synthesis protein cobW 3061138 3060086 −3 1053 TM.orf2761btuB receptor 3061646 3063613 3 1968 TM.orf2762 fdx4 Ferredoxin, 2Fe—2S3063597 3064358 2 762 TM.orf2763 periplasmic binding protein 30643553065317 3 963 TM.orf2764 yjbI protozoan/cyanobacterial globin familyprotein 3065328 3065810 2 483 TM.orf2765 putative transcriptionalregulator 3066493 3065927 −3 567 TM.orf2766 rhtA Inner membranetransporter rhtA 3066596 3067492 3 897 TM.orf2767 cobB Cobyrinic acida,c-diamide synthase CbiA 3068814 3067474 −1 1341 TM.orf2768 cobAputative uroporphyrin-III C-methyltransferase 3069710 3068811 −2 900protein TM.orf2769 cbiD cobalt-precorrin-6A synthase 3069779 3070960 31182 TM.orf2770 cobK precorrin-6x reductase 3070965 3071759 2 795TM.orf2771 hupE HupE/UreJ protein 3072327 3071746 −1 582 TM.orf2772 cobMprecorrin-4 C11-methyltransferase 3073264 3072419 −3 846 TM.orf2773 cobJprecorrin-3B C17-methyltransferase (cobJ) 3075147 3073261 −1 1887TM.orf2774 cobI precorrin 2 methylase 3075887 3075144 −2 744 TM.orf2775cobL Uroporphyrin-III C/tetrapyrrole 3077104 3075866 −3 1239(Corrin/Porphyrin) methyltransferase TM.orf2776 cobH Precorrin-8Xmethylmutase 3077739 3077101 −1 639 TM.orf2777 cbiX Sirohydrochlorincobaltochelatase 3078893 3077736 −2 1158 TM.orf2778 atpI ATP synthaseprotein I 3079616 3080020 3 405 TM.orf2779 atpB F0F1-type ATP synthase,subunit a 3080135 3080866 3 732 TM.orf2780 atpE ATP synthase C chain3080986 3081213 1 228 TM.orf2781 H + transporting two-sector ATPase B/B′3081431 3081934 3 504 subunit TM.orf2782 atpF ATP synthase B chainprecursor 3081940 3082422 1 483 TM.orf2783 icfG protein serine/threoninephosphatase 3082791 3084833 2 2043 TM.orf2784 ABC-type transport system,periplasmic 3084830 3086056 3 1227 component TM.orf2785 conservedhypothetical protein 3087609 3086035 −1 1575 TM.orf2786 putative innermembrane transport protein 3089987 3087606 −2 2382 TM.orf2787 glycinedehydrogenase subunit 2 3091764 3090199 −1 1566 TM.orf2788 glycinedehydrogenase subunit 1 3093125 3091761 −2 1365 TM.orf2789 gcvH glycinecleavage system H protein 3093510 3093133 −1 378 TM.orf2790 gcvT glycinecleavage system T protein 3094692 3093553 −1 1140 TM.orf2791 conservedhypothetical protein 3095752 3095186 −3 567 TM.orf2792 CDGSH iron sulfurdomain-containing protein 3 3096209 3095940 −2 270 TM.orf2793 ispH4-hydroxy-3-methylbut-2-enyl diphosphate 3096503 3097483 3 981 reductaseTM.orf2794 thrB Homoserine kinase ThrB 3097567 3098532 1 966 TM.orf2795rnhA ribonuclease H 3098519 3098974 3 456 TM.orf2796 upp uracilphosphoribosyltransferase 3099673 3098999 −3 675 TM.orf2797 Redoxindomain protein 3100282 3099800 −3 483 TM.orf2798 dsbD Thiol: disulfideinterchange protein 3102643 3100418 −3 2226 TM.orf2799 transcriptionalregulator 3102915 3103508 2 594 TM.orf2800 conserved hypotheticalprotein 3104602 3103775 −3 828 TM.orf2801 rimJ GCN5-relatedN-acetyltransferase 3105314 3104664 −2 651 TM.orf2802 tusA SirA-likeprotein 3105687 3105343 −1 345 TM.orf2803 M16 family peptidase 31070033105726 −2 1278 TM.orf2804 thrC threonine synthase 3108468 3107065 −11404 TM.orf2805 Thermostable carboxypeptidase 1 3110179 3108641 −3 1539TM.orf2806 ctaA cytochrome C oxidase assembly protein, AA3 31112193110215 −1 1005 subunit-controlling protein TM.orf2807 SURF1 familyprotein 3111981 3111298 −1 684 TM.orf2808 conserved hypothetical protein3112341 3112093 −1 249 TM.orf2809 putative cytochrome c oxidasepolypeptide III 3113309 3112500 −2 810 (cytochrome aa3 subunit 3)TM.orf2810 ctaG cytochrome-c oxidase assembly protein 3114153 3113482 −1672 TM.orf2811 hypothetical protein 3114413 3114198 −2 216 TM.orf2812ctaB putative protoheme IX farnesyltransferase 3115450 3114410 −3 1041(heme o synthase) TM.orf2813 ctaD cytochrome c oxidase, subunit I3117072 3115573 −1 1500 TM.orf2814 ctaC cytochrome C oxidase subunit II3118046 3117207 −2 840 TM.orf2815 peptidase U62 modulator of DNA gyrase3118594 3120039 1 1446 TM.orf2816 TRAP transporter, 4TM/12TM fusionprotein 3122292 3120118 −1 2175 TM.orf2817 TRAP transporter solutereceptor, TAXI family 3123381 3122419 −1 963 TM.orf2818 conservedhypothetical protein 3124273 3123434 −3 840 TM.orf2819 yfhH RpiR familytranscriptional regulator 3125326 3124340 −3 987 TM.orf2820aminotransferase class-III 3126719 3125340 −2 1380 TM.orf2821 putativeglutamatecysteine ligase 3128133 3126892 −1 1242 TM.orf2822 rsmEribosomal RNA small subunit 3129106 3128333 −3 774 methyltransferaseTM.orf2823 Coq2 4-hydroxybenzoate polyprenyltransferase and 31292543130210 3 957 related prenyltransferases TM.orf2824 hypothetical protein3130457 3130176 −2 282 TM.orf2825 ubiD 3-octaprenyl-4-hydroxybenzoatecarboxy- 3130544 3132064 3 1521 lyase TM.orf2826 conserved hypotheticalprotein 3132143 3133906 3 1764 TM.orf2827 hypothetical protein 31339633134148 1 186 TM.orf2828 metallophosphoesterase 3134264 3135094 3 831TM.orf2829 pmbA Peptidase U62, modulator of DNA gyrase 3135206 3136573 31368 TM.orf2830 suhB inositol-phosphate phosphatase 3136627 3137493 1867 TM.orf2831 msbA ABC-type multidrug transport system, ATPase 31374473139429 3 1983 and permease components TM.orf2832 conserved hypotheticalprotein 3139435 3140166 1 732 TM.orf2833 waaAthree-deoxy-D-manno-octulosonic-acid 3140163 3141497 2 1335 transferaseTM.orf2834 lpxK Tetraacyldisaccharide 4′-kinase 3141494 3142582 3 1089TM.orf2835 Lauroyl/myristoyl acyltransferase 3142579 3143499 1 921TM.orf2836 hypothetical protein 3144007 3143807 −3 201 TM.orf2837hypothetical protein 3144272 3144619 3 348 TM.orf2838 Putative amidase3146122 3144731 −3 1392 TM.orf2877 cytidine/deoxycytidylate deaminase,zinc- 3185617 3185183 −3 435 binding region TM.orf2878 possiblegamma-glutamyl-gamma- 3185753 3186490 3 738 aminobutyrate hydrolaseTM.orf2879 pseudouridine synthase, Rsu 3186487 3188253 1 1767 TM.orf2880N6-adenine-specific methylase 3188260 3188895 1 636 TM.orf2881 conservedhypothetical protein 3188997 3189890 2 894 TM.orf2882 metC cystathioninebeta-lyase 3191071 3189935 −3 1137 TM.orf2883 Biotin synthase 1 31927833191149 −1 1635 TM.orf2884 conserved hypothetical protein 31942173193033 −1 1185 TM.orf2885 mutL DNA mismatch repair protein MutL 31961453194217 −2 1929 TM.orf2886 pgi Glucose-6-phosphate isomerase 31974433196160 −3 1284 TM.orf2887 protease 3198930 3197473 −1 1458 TM.orf2888peptidase, M16 family 3200453 3198927 −2 1527 TM.orf2889 conservedhypothetical protein 3201124 3200570 −3 555 TM.orf2890 lspA signalpeptidase II 3201750 3201214 −1 537 TM.orf2891 ileS isoleucyl-tRNAsynthetase 3204559 3201743 −3 2817 TM.orf2892 ribF FAD synthase 32056643204687 −2 978 TM.orf2893 MaoC-like dehydratase 3206193 3205744 −1 450TM.orf2894 epoxide hydrolase 3207378 3206482 −1 897 TM.orf2895hypothetical protein 3207569 3207859 3 291 TM.orf2896 ybjE conservedhypothetical protein 3207856 3208455 1 600 TM.orf2897 Alcoholdehydrogenase zinc-binding domain 3209452 3208460 −3 993 proteinTM.orf2898 ydeS TetR family transcriptional regulator 3210229 3209510 −3720 TM.orf2899 emrB major facilitator superfamily permease 32118273210226 −1 1602 TM.orf2900 emrA secretion protein HlyD 3212940 3211843−1 1098 TM.orf2901 hypothetical protein 3213113 3212973 −2 141TM.orf2902 sigK RNA polymerase sigma factor 3214108 3214683 1 576TM.orf2903 conserved hypothetical protein 3214680 3215468 2 789TM.orf2904 extracellular solute-binding protein 3215562 3216545 2 984TM.orf2905 iron(III) transport system permease protein 3216650 3218272 31623 TM.orf2906 ugpC ABC transporter component 3218269 3219384 1 1116TM.orf2907 FAD dependent oxidoreductase 3220579 3219395 −3 1185TM.orf2908 TetR family transcriptional regulator 3220742 3221344 3 603TM.orf2909 map methionine aminopeptidase, type I, Map 3221341 3222165 1825 putative TM.orf2910 conserved hypothetical protein 3222268 3223383 11116 TM.orf2911 tsr putative methyl-accepting chemotaxis protein 32235433225231 1 1689 TM.orf2912 putative methyl-accepting chemotaxis protein3225458 3227146 3 1689 TM.orf2913 conserved hypothetical protein 32271813227645 2 465 TM.orf2914 conserved hypothetical protein 3228498 3227650−1 849 TM.orf2915 eutB ethanolamine ammonia-lyase heavy chain 32286833230098 3 1416 TM.orf2916 eutC Ethanolamine ammonia-lyase 32300953230928 1 834 TM.orf2917 ycaQ conserved hypothetical protein 32321533230915 −3 1239 TM.orf2918 putative lactonase precursor 3232435 32334721 1038 TM.orf2919 rimJ GCN5-related N-acetyltransferase 3233568 32340982 531 TM.orf2920 hmuV ABC transporter related protein 3235006 3234191 −3816 TM.orf2921 transport system permease protein 3236055 3235006 −1 1050TM.orf2922 periplasmic binding protein 3237179 3236052 −2 1128TM.orf2923 conserved hypothetical protein 3237469 3237176 −3 294TM.orf2924 cirA TonB-dependent receptor 3239539 3237536 −3 2004TM.orf2925 nahR Transcriptional regulator, LysR family 3240606 3239626−1 981 TM.orf2926 degU response regulator 3240884 3241528 3 645TM.orf2927 yxjM putative signal transduction histidine kinase 32415253243357 1 1833 TM.orf2928 truncated FmtB 3243493 3244641 1 1149TM.orf2929 hypothetical protein 3244953 3246122 2 1170 TM.orf2930conserved hypothetical protein 3246119 3247663 3 1545 TM.orf2931 adhAalcohol dehydrogenase 3248702 3247671 −2 1032 TM.orf2932 hypotheticalprotein 3248839 3248681 −3 159 TM.orf2933 acoR Fis family GAF modulatedsigma54 specific 3249372 3251333 2 1962 transcriptional regulatorTM.orf2934 Cobalt transport protein ATP-binding subunit 3252222 3251398−1 825 TM.orf2935 cobalt transport integral membrane protein 32525243252222 −2 303 TM.orf2936 cobalt ABC transporter, inner membrane 32529793252521 −3 459 subunit CbiQ TM.orf2937 cbiN cobalt transport protein3253308 3252979 −1 330 TM.orf2938 cbiM cobalamin biosynthesis proteincbim 3253979 3253305 −2 675 TM.orf2939 conserved hypothetical protein3254522 3254896 3 375 TM.orf2940 putative RNA polymerase, sigma-24subunit, 3254893 3256143 1 1251 ECF subfamily TM.orf2941 hypotheticalprotein 3256528 3256127 −3 402 TM.orf2942 slmA transcriptionalregulatory protein 3256620 3257273 2 654 TM.orf2943 OxidoreductaseFAD/NAD(P)-binding 3258577 3257249 −3 1329 TM.orf2944 slyA MarR familytranscriptional regulator 3259153 3258638 −3 516 TM.orf2945 membraneprotein of unknown function 3260016 3259150 −1 867 TM.orf2946 yecEconserved hypothetical protein 3261081 3261875 2 795 TM.orf2947 yhaZconserved hypothetical protein 3262984 3261872 −3 1113 TM.orf2948conserved hypothetical protein 3263683 3263087 −3 597 TM.orf2949 hutUurocanate hydratase 3265396 3263723 −3 1674 TM.orf2950 hutHphenylalanine/histidine ammonia-lyase 3267021 3265471 −1 1551 TM.orf2951putative glycine betaine/L-proline transport 3267847 3267026 −3 822 ATPbinding subunit TM.orf2952 proW histidine transport system permease ABC3268697 3267840 −2 858 transporter protein TM.orf2953 proX Glycinebetaine-binding periplasmic protein 3269858 3268815 −2 1044 TM.orf2954hutC GntR family transcriptional regulator 3270101 3270859 3 759TM.orf2955 mdeA methionine gamma-lyase 3272131 3270926 −3 1206TM.orf2956 lrp transcriptional regulator, AsnC family 3272249 3272707 3459 TM.orf2957 transglutaminase domain protein 3272807 3273718 3 912TM.orf2958 2-hydroxy-3-oxopropionate reductase 3273870 3274793 2 924TM.orf2959 ybfI transcriptional regulator, AraC family 3275542 3274745−3 798 TM.orf2960 conserved hypothetical protein 3275668 3276291 1 624TM.orf2961 plasmid stabilization system 3276601 3276302 −3 300TM.orf2962 addiction module antidote protein, CC2985 3276854 3276591 −2264 family TM.orf2963 yddQ putative hydrolase 3277476 3276931 −1 546TM.orf2964 glxA AraC family transcriptional regulator 3277598 3278584 3987 TM.orf2965 rplU 50S ribosomal protein L21P 3279122 3279430 3 309TM.orf2966 rpmA 50S ribosomal protein L27 3279545 3279805 3 261TM.orf2967 obg GTP-binding protein Obg/CgtA 3280029 3281519 2 1491TM.orf2968 proB gamma-glutamyl kinase 3281516 3282721 3 1206 TM.orf2969proA gamma-glutamyl phosphate reductase 3282718 3284061 1 1344TM.orf2970 nadD nicotinate (nicotinamide) nucleotide 3284126 3284737 3612 adenylyltransferase TM.orf2971 ybeB lojap-related protein 32848653285269 2 405 TM.orf2972 rlmH Ribosomal RNA large subunit 32853973285870 1 474 methyltransferase H TM.orf2973 conserved hypotheticalprotein 3285905 3286588 3 684 TM.orf2974 gpmI phosphoglycerate mutase3286725 3288329 2 1605 TM.orf2975 yibP Peptidase M23B 3288334 3289740 11407 TM.orf2976 ctpA carboxyl-terminal processing protease 32898343291231 1 1398 TM.orf2977 conserved hypothetical protein 3291269 32925793 1311 TM.orf2978 rppH nudix family hydrolase 3292662 3293138 2 477TM.orf2979 modA molybdate ABC transporter, periplasmic 3293246 3294043 3798 molybdate-binding protein TM.orf2980 modB molybdate ABC transporter,permease protein 3294078 3294839 2 762 TM.orf2981 modC molybdatetransporter ATP-binding protein 3294836 3295957 3 1122 TM.orf2982 mopBputative molybdenum-binding transcriptional 3296390 3295932 −2 459regulator ModE family protein TM.orf2983 conserved hypothetical protein3297205 3296390 −3 816 TM.orf2984 atpC F-type H+-transporting ATPaseepsilon chain 3297891 3297490 −1 402 TM.orf2985 atpD F1 -ATP SynthaseBeta Chain 3299373 3297949 −1 1425 TM.orf2986 atpG F-typeH+-transporting ATPase gamma chain 3300342 3299449 −1 894 TM.orf2987atpA primosome assembly protein PriA 3302050 3300515 −3 1536 TM.orf2988atpH F0F1 ATP synthase subunit delta 3302607 3302047 −1 561 TM.orf2989primosome assembly protein PriA 3305120 3302916 −2 2205 TM.orf2990 taltransaldolase 3305466 3306122 2 657 TM.orf2991 conserved hypotheticalprotein 3306180 3306977 2 798 TM.orf2992 xerC Tyrosine recombinase xerC3306962 3307984 3 1023 TM.orf2993 pcoA multicopper oxidase 33094203307975 −1 1446 TM.orf2994 hypothetical protein 3309811 3309482 −3 330TM.orf2995 crp transcriptional regulator, Crp 3310559 3309834 −2 726TM.orf2996 hypothetical protein 3312135 3310825 −1 1311 TM.orf2997 lolDABC transporter, ATP-binding protein 3312949 3312218 −3 732 TM.orf2998efflux ABC transporter, permease protein 3314076 3312949 −1 1128TM.orf2999 auxiliary transport protein, MFP family 3315350 3314073 −21278 TM.orf3000 hypothetical protein 3315558 3315881 2 324 TM.orf3001rpoE RNA polymerase sigma-70 factor, ECF 3315994 3316557 1 564 subfamilyTM.orf3002 conserved hypothetical protein 3316554 3317003 2 450TM.orf3003 conserved hypothetical protein 3317000 3317461 3 462TM.orf3004 LPD1 dihydrolipoamide dehydrogenase 3318981 3317569 −1 1413TM.orf3005 sucB 2-oxoglutarate dehydrogenase, E2 3320307 3319057 −1 1251component, dihydrolipoamide succinyltransferase TM.orf3006 sucA2-oxoglutarate dehydrogenase E1 component 3323323 3320432 −3 2892TM.orf3007 sucD Succinyl-CoA synthetase alpha chain 3324264 3323389 −1876 TM.orf3008 sucC succinyl-CoA synthetase, beta subunit 33254363324267 −2 1170 TM.orf3009 mdh malate dehydrogenase 3326560 3325616 −3945 TM.orf3010 AFG1 family ATPase 3328140 3326962 −1 1179 TM.orf3011ywrD gamma-glutamyltranspeptidase 3328425 3329972 2 1548 TM.orf3012 asdaspartate-semialdehyde dehydrogenase 3330149 3331141 3 993 TM.orf3013paiB Protease synthase and sporulation protein PAI 3331307 3331975 3 669TM.orf3014 hypothetical protein 3332533 3331976 −3 558 TM.orf3015 dppFDppF 3333765 3332776 −1 990 TM.orf3016 dppD dipeptide ABC superfamilyATP binding 3334762 3333758 −3 1005 cassette transporter, ABC proteinTM.orf3017 dppC peptide/nickel transport system permease 3335658 3334762−1 897 protein TM.orf3018 dppB nickel-transporting ATPase 33367503335740 −1 1011 TM.orf3019 dppA dipeptide-binding ABC transportersubstrate- 3338498 3336903 −2 1596 binding component TM.orf3020 yqjGputative glutathione S-transferase 3339743 3338727 −2 1017 TM.orf3021Flavoprotein WrbA 3340444 3339830 −3 615 TM.orf3022 transcriptionalregulator 3340592 3341506 3 915 TM.orf3023 conserved hypotheticalprotein 3341543 3341980 3 438 TM.orf3024 putative hydrolase 33420813342911 2 831 TM.orf3025 yerD glutamate synthase 3344576 3342924 −2 1653TM.orf3026 dapB Dihydrodipicolinate reductase, bacterial 3345433 3344573−3 861 TM.orf3027 two-component hybrid sensor and regulator 33471283345491 −3 1638 TM.orf3028 gitA citrate synthase I 3348662 3347349 −21314 TM.orf3029 conserved hypothetical protein 3349785 3348889 −1 897TM.orf3030 cytochrome c, class I 3350426 3349782 −2 645 TM.orf3031hypothetical protein 3351351 3350485 −1 867 TM.orf3032 phenylacetic aciddegradation-like protein 3351529 3351951 1 423 TM.orf3033 histidinetriad protein 3352065 3352511 2 447 TM.orf3034 cya adenylate/guanylatecyclase 3352516 3353817 1 1302 TM.orf3035 ribosomal large subunitpseudouridine 3354697 3353828 −3 870 synthase D TM.orf3036endoribonuclease L-PSP 3354836 3355204 3 369 TM.orf3037 nudI putativeNUDIX hydrolase 3355213 3355674 1 462 TM.orf3038 CMO dioxygenase 33568603355649 −3 1212 TM.orf3039 putative lactone-dependent transcriptional3357527 3356826 −2 702 regulator (tetr-family), mmfr TM.orf3040 ytcBNAD-dependent epimerase/dehydratase 3357911 3358969 3 1059 TM.orf3041dTDP-4-dehydrorhamnose 3,5-epimerase 3358977 3359543 2 567 TM.orf3042NAD dependent epimerase/dehydratase family 3359540 3360484 3 945TM.orf3043 oxidoreductase 3360565 3361692 1 1128 TM.orf3044 crtenoyl-CoA hydratase/isomerase 3361740 3362543 2 804 TM.orf3045 conservedhypothetical protein 3362634 3363560 2 927 TM.orf3046 LmbE-like protein3364894 3364127 −3 768 TM.orf3047 Acyl-CoA dehydrogenase 3366072 3364888−1 1185 TM.orf3048 glycosyl transferase family 2 3367241 3366069 −2 1173TM.orf3049 PIP proline iminopeptidase 3368566 3367547 −3 1020 TM.orf3050yfgC peptidase M48 Ste24p 3368909 3370357 3 1449 TM.orf3051 thioesterasesuperfamily protein 3370850 3370371 −2 480 TM.orf3052 thioesterasesuperfamily protein 3371302 3370868 −3 435 TM.orf3053 rpoH RNApolymerase sigma-32 factor 3372489 3371590 −1 900 TM.orf3054 ydeBTranscriptional regulators, similar to 3373223 3372726 −2 498 M. xanthusCarD TM.orf3055 fdxA 4Fe—4S ferredoxin iron-sulfur binding domain-3374015 3373677 −2 339 containing protein TM.orf3056 hslR heat shockprotein 3374698 3374264 −3 435 TM.orf3057 Helicase-like protein 33773373374695 −1 2643 TM.orf3058 htpG TPG_MAGMM RecName: Full = Chaperone3377583 3379529 2 1947 protein htpG; AltName: Full = Heat shock proteinhtpG; AltName: Full = High temperature protein G TM.orf3059 ycaD majorfacilitator transporter 3381061 3379517 −3 1545 TM.orf3060 acnAaconitate hydratase 3381716 3381291 −2 426 TM.orf3061 acn aconitatehydratase 3383994 3381724 −1 2271 TM.orf3062 braF high-affinitybranched-chain amino acid 3384871 3385668 1 798 transport ATP-bindingprotein TM.orf3063 Long-chain acyl-CoA synthetases (AMP- 3385665 33875962 1932 forming) TM.orf3064 braD inner-membrane translocator 33876963388589 2 894 TM.orf3065 IivM branched-chain amino acid transport system3388609 3389670 1 1062 permease protein TM.orf3066 branched-chain aminoacid transport system 3389828 3391060 3 1233 substrate-binding proteinTM.orf3067 IivF ABC transporter related protein 3391149 3392006 2 858TM.orf3068 paaK phenylacetate--CoA ligase 3392075 3393316 3 1242TM.orf3069 hpsA 3-hexulose-6-phosphate synthase 3394166 3393423 −2 744TM.orf3070 dihydroxy-acid dehydratase 3395893 3394163 −3 1731 TM.orf3071hypothetical protein 3396063 3395899 −1 165 TM.orf3072 trapdicarboxylate transporter- dctm subunit 3397390 3396074 −3 1317TM.orf3073 TRAP-type mannitol/chloroaromatic compound 3397901 3397380 −2522 transport system, small permease component TM.orf3074 yiiZ TRAPdicarboxylate family transporter, DctP 3398998 3397976 −3 1023 subunitTM.orf3075 transcriptional regulatory protein 3399123 3399842 2 720TM.orf3076 hypothetical protein 3400517 3399870 −2 648 TM.orf3077 ccmAheme ABC exporter, ATP-binding protein 3400672 3401334 1 663 CcmATM.orf3078 helB heme exporter protein CcmB 3401331 3402005 2 675TM.orf3079 cycZ heme exporter protein CcmC 3402323 3402895 3 573TM.orf3080 hypothetical protein 3403067 3403330 3 264 TM.orf3081 ccmECcmE/CycJ protein 3403314 3403874 2 561 TM.orf3082 cycK cytochrome cbiogenesis factor 3403874 3405889 3 2016 TM.orf3083 helX cytochrome cbiogenesis protein 3405902 3406438 3 537 TM.orf3084 ccmH proteininvolved in biosynthesis of c-type 3406435 3407139 1 705 cytochromesTM.orf3085 cycH cytochrome c-type biogenesis protein CycH 34071363408560 2 1425 TM.orf3086 aotP Arginine/ornithine transport ATP-binding3408690 3409466 2 777 protein aotP TM.orf3087 petP MarR familytranscriptional regulator 3409604 3410125 3 522 TM.orf3088 occTextracellular solute-binding protein family 3 3410322 3411095 2 774TM.orf3089 hisQ polar amino acid ABC transporter, inner 3411236 34119583 723 membrane subunit TM.orf3090 occM polar amino acid transport systempermease 3411962 3412648 3 687 protein TM.orf3091 yisB conservedhypothetical protein 3412717 3412950 1 234 TM.orf3092 IivHbranched-chain amino acid ABC transporter 3413234 3414094 3 861(permease) TM.orf3093 braE inner-membrane translocator 3414099 3415142 21044 TM.orf3094 braF branched-chain amino acid ABC transporter 34151393415915 3 777 ATP-binding protein TM.orf3095 IivF branched-chain aminoacid ABC transporter 3415902 3416609 2 708 ATP-binding proteinTM.orf3096 extracellular ligand-binding receptor 3416695 3417903 1 1209TM.orf3097 queF GTP cyclohydrolase family protein 3418466 3417993 −2 474TM.orf3098 conserved hypothetical protein 3418835 3419149 3 315TM.orf3099 conserved hypothetical protein 3419154 3419504 2 351TM.orf3100 folD FolD bifunctional protein 3419570 3420466 3 897TM.orf3101 conserved hypothetical protein 3420498 3421088 2 591TM.orf3102 conserved hypothetical protein 3421639 3421070 −3 570TM.orf3103 conserved hypothetical protein 3421938 3421645 −1 294TM.orf3104 hss homospermidine synthase 3423511 3422096 −3 1416TM.orf3105 argG argininosuccinate synthase 3424922 3423708 −2 1215TM.orf3106 Nephrocystin-3 3427879 3425117 −3 2763 TM.orf3107 cyaAadenylate cyclase, family 3 3429289 3427979 −3 1311 TM.orf3108Glutathione S-transferase domain protein 3429468 3430160 2 693TM.orf3109 beta-lactamase domain-containing protein 3430166 3431068 3903 TM.orf3110 pqqE pyrroloquinoline quinone biosynthesis protein3432137 3431073 −2 1065 PqqE TM.orf3111 coenzyme PQQ synthesis D 34324243432134 −3 291 TM.orf3112 pqqC pyrroloquinoline quinone biosynthesisprotein 3433185 3432421 −1 765 PqqC TM.orf3113 pqqB coenzyme PQQbiosynthesis protein B 3434081 3433182 −2 900 TM.orf3114 hypotheticalprotein 3434225 3434100 −2 126 TM.orf3115 rlmN cfr family radical SAMenzyme 3435617 3434352 −2 1266 TM.orf3116 conserved hypothetical protein3436238 3435684 −2 555 TM.orf3117 conserved hypothetical protein 34365133437232 1 720 TM.orf3118 cya2 adenylyl cyclase class-3/4/guanylylcyclase 3437330 3438481 3 1152 TM.orf3119 conserved hypothetical protein3439105 3440133 1 1029 TM.orf3120 purH bifunctional 3440241 3441824 21584 phosphoribosylaminoimidazolecarboxamide formyltransferase/IMPcyclohydrolase TM.orf3121 hypothetical protein 3441914 3442384 3 471TM.orf3122 conserved hypothetical protein 3442872 3442390 −1 483TM.orf3123 conserved hypothetical protein 3443297 3442869 −2 429TM.orf3124 NAD-glutamate dehydrogenase 3448211 3443370 −2 4842TM.orf3125 conserved hypothetical protein 3449196 3448420 −1 777TM.orf3126 mhbM 3-hydroxybenzoate 6-hydroxylase 3450939 3449662 −1 1278TM.orf3127 MarR family transcriptional regulator 3451172 3451669 3 498TM.orf3128 conserved hypothetical protein 3451809 3452450 2 642TM.orf3129 TRAP dicarboxylate transporter- DctM subunit 3452447 34537663 1320 TM.orf3130 conserved hypothetical protein 3453790 3454284 1 495TM.orf3131 siaP TRAP dicarboxylate transporter- DctP subunit 34543603455430 1 1071 TM.orf3132 Ala-tRNA(Pro) hydrolase 3455535 3456035 2 501TM.orf3133 trxA thioredoxin 3456220 3457137 1 918 TM.orf3134 IonDPeptidase S16, Ion-like protein 3457253 3457921 3 669 TM.orf3135conserved hypothetical protein 3457941 3458153 2 213 TM.orf3136conserved hypothetical protein 3458158 3458556 1 399 TM.orf3137Ubiquinone biosynthesis hydroxylase, 3460270 3459056 −3 1215UbiH/UbiF/VisC/COQ6 TM.orf3138 glnB nitrogen regulatory protein P-II3460624 3460962 1 339 TM.orf3139 ammonium transporter 3460989 3462314 21326 TM.orf3140 aatC aminotransferase class I and II 3462541 3463752 11212 TM.orf3141 ftsK DNA segregation ATPase FtsK/SpoIIIE 3463826 34662433 2418 TM.orf3142 lolA Outer membrane lipoprotein carrier protein3466269 3466949 2 681 LolA TM.orf3143 Na+-driven multidrug efflux pump3466974 3468320 2 1347 TM.orf3144 puuD putative glutamineamidotransferase 3468377 3469162 3 786 TM.orf3145 conserved hypotheticalprotein 3469186 3469800 1 615 TM.orf3146 alsT putative amino acidcarrier transmembrane 3469958 3471373 3 1416 protein TM.orf3147intracellular PHB depolymerase 3472706 3471480 −2 1227 TM.orf3148 sco1Classical-complement-pathway C3/C5 3473694 3473074 −1 621 convertaseTM.orf3149 cobD Cobalamin biosynthesis protein 3473990 3475003 3 1014TM.orf3150 SH3 domain-containing YSC84-like protein 3475748 3475056 −2693 TM.orf3151 cobP Adenosylcobinamide-phosphate 3476453 3475914 −2 540guanylyltransferase TM.orf3152 Phosphoglycerate mutase 3477076 3476447−3 630 TM.orf3153 hypothetical protein 3477915 3477067 −1 849 TM.orf3154cobT nicotinate-nucleotide--dimethylbenzimidazole 3478146 3479183 2 1038TM.orf3155 yddV diguanylate cyclase 3479436 3480347 2 912 TM.orf3156sigK RNA polymerase sigma-70 factor, ECF family 3480622 3481158 1 537protein TM.orf3157 chrR Anti-sigma factor ChrR 3481158 3481880 2 723TM.orf3158 conserved hypothetical protein 3481960 3482967 1 1008TM.orf3159 lpsJ 3-oxoadipate CoA-transferase, beta subunit 34836933483052 −1 642 TM.orf3160 lpsI 3-oxoadipate CoA-transferase subunit A3484379 3483696 −2 684 TM.orf3161 sam RNA polymerase sigma-54 factor3484716 3485678 2 963 TM.orf3162 hypothetical protein 3485675 3486301 3627 TM.orf3163 3-hydroxyacyl-CoA dehydrogenase type-2 3486519 3487283 2765 TM.orf3164 pterin-4-alpha-carbinolamine dehydratase 3487679 3487371−2 309 TM.orf3165 conserved hypothetical protein 3487806 3488243 2 438TM.orf3166 ybgC Acyl-CoA thioester hydrolase ybgC 3488700 3488275 −1 426TM.orf3167 conserved hypothetical protein 3489298 3489618 1 321TM.orf3168 gltX glutamyl-tRNA synthetase, class Ic 3490728 3489820 −1909 TM.orf3169 mhpE pyruvate carboxyltransferase 3491016 3492002 2 987TM.orf3170 PqqC-like protein 3492074 3492838 3 765 TM.orf3171 ilvEbranched-chain amino acid aminotransferase- 3492931 3493884 1 954 likeprotein TM.orf3172 conserved hypothetical protein 3493934 3495322 3 1389TM.orf3173 dlpA Dimethylmenaquinone methyltransferase 3495385 3496095 1711 TM.orf3174 divJ putative non-motile and phage-resistance 34962223497631 1 1410 protein TM.orf3175 HNH endonuclease family protein3497817 3498515 2 699 TM.orf3176 aspC possible aspartate transaminase3498661 3499797 1 1137 TM.orf3177 thiE thiamine-phosphatepyrophosphorylase 3500432 3499794 −2 639 TM.orf3178 thiMHydroxyethylthiazole kinase 3501238 3500429 −3 810 TM.orf3179 coq7ubiquinone biosynthesis protein COQ7 3502079 3501501 −2 579 TM.orf3180disulfide bond formation protein, DsbB family 3502650 3502105 −1 546TM.orf3181 TRAP transporter solute receptor TAXI family 3502874 35041543 1281 protein TM.orf3182 pepB Leucyl aminopeptidase 3504275 3505681 31407 TM.orf3183 conserved hypothetical protein 3505814 3507070 3 1257TM.orf3184 ada transcriptional regulator, AraC family 3508283 3507045 −21239 TM.orf3185 yejF putative ATP-binding component of ABC 35100383508401 −2 1638 transporter TM.orf3186 yejE binding-protein-dependenttransport systems 3511088 3510180 −2 909 inner membrane componentTM.orf3187 yejB peptide/nickel transport system permease 3512177 3511092−2 1086 protein TM.orf3188 ABC-type oligopeptide transport system,3514064 3512196 −2 1869 periplasmic component TM.orf3189 Inositolmonophosphatase 3514926 3514123 −1 804 TM.orf3190 cycM putativeCytoChrome c-like protein 3515700 3515083 −1 618 TM.orf3191 kdsB3-deoxy-manno-octulosonate 3516147 3516881 2 735 cytidylyltransferaseTM.orf3192 ADT1 prephenate dehydratase 3516989 3517876 3 888 TM.orf3193qacE small multidrug resistance protein 3517958 3518305 3 348 TM.orf3194ydcN transcriptional regulator, XRE family with cupin 3519017 3518373 −2645 sensor TM.orf3195 ilvE branched-chain amino acid aminotransferase3520135 3519266 −3 870 TM.orf3196 ybbP ABC transporter permease ybbP3523075 3520439 −3 2637 TM.orf3197 ybbA ABC transporter related protein3523761 3523072 −1 690 TM.orf3198 tesA SGNH hydrolase-type arylesterase3524000 3524653 3 654 TM.orf3199 carbon monoxide dehydrogenase subunit G3524776 3525519 1 744 TM.orf3200 transferase hexapeptide repeatcontaining 3526095 3525565 −1 531 protein TM.orf3201 L-carnitinedehydratase/bile acid-inducible 3527392 3526133 −3 1260 protein FTM.orf3202 yngG pyruvate carboxyltransferase 3528387 3527440 −1 948TM.orf3203 benM transcriptional regulator, LysR family 3529436 3528513−2 924 TM.orf3204 conserved hypothetical protein 3529858 3529433 −3 426TM.orf3205 BphI lactonase 3530062 3530904 1 843 TM.orf3206 conservedhypothetical protein 3531622 3530864 −3 759 TM.orf3207 conservedhypothetical protein 3531763 3532068 1 306 TM.orf3208 conservedhypothetical protein 3532080 3532586 2 507 TM.orf3209 yadG ABC-typemultidrug transport system, ATPase 3534029 3533049 −2 981 componentTM.orf3210 lbsA Lactobacillus shifted protein 3534259 3534438 1 180TM.orf3211 polA DNA polymerase I 3534664 3537480 1 2817 TM.orf3212HhH-GPD family protein 3537477 3538469 2 993 TM.orf3213 conservedhypothetical protein 3538466 3538942 3 477 TM.orf3214 conservedhypothetical protein 3538974 3540389 2 1416 TM.orf3215 hypotheticalprotein 3540683 3540396 −2 288 TM.orf3216 conserved hypothetical protein3541151 3540885 −2 267 TM.orf3217 hspH heat shock protein, Hsp20 family3541701 3541243 −1 459 TM.orf3218 Transcription initiation factorTFIIIB, Brf1 3542719 3542366 −3 354 subunit/Transcription initiationfactor TFIIB TM.orf3219 aroA 3-phosphoshikimate1-carboxyvinyltransferase 3543011 3544378 3 1368 TM.orf3220 cmkcytidylate kinase 3544403 3545047 3 645 TM.orf3221 rpsA Ribosomalprotein S1 3545443 3547167 1 1725 TM.orf3222 HD domain protein 35473833547958 2 576 TM.orf3223 sulfatase 3547999 3549504 1 1506 TM.orf3224Abortive infection bacteriophage resistance 3550482 3549514 −1 969protein Abi TM.orf3225 cbbR putative transcriptional regulator 35514693550579 −1 891 TM.orf3226 ugpB sn-glycerol-3-phosphate-bindingperiplasmic 3551620 3552942 1 1323 protein TM.orf3227 ugpAsn-glycerol-3-phosphate ABC transporter 3553017 3553922 2 906 permeaseTM.orf3228 ugpE sn-glycerol-3-phosphate transport system 3553909 35548381 930 permease protein TM.orf3229 sppA signal peptide peptidase SppA,36K type 3554947 3555903 1 957 TM.orf3230 ihfB Bacterial nucleoidDNA-binding protein 3556054 3556335 1 282 TM.orf3231 hypotheticalprotein 3556477 3556851 1 375 TM.orf3232 pyrF orotidine-5′-phosphatedecarboxylase 3557039 3557800 3 762 TM.orf3233 trpFN-(5′phosphoribosyl)anthranilate isomerase 3557804 3558508 3 705TM.orf3234 trpB tryptophan synthase subunit beta 3558529 3559743 1 1215TM.orf3235 trpA RPA_AZOBR RecName: Full = Tryptophan 3559740 3560612 2873 synthase alpha chain gb TM.orf3236 accD acetyl-CoA carboxylasecarboxyl transferase 3560668 3561687 1 1020 subunit beta TM.orf3237 FOL3folC bifunctional protein 3561746 3563083 3 1338 TM.orf3238 ytxMcarboxyl esterase 3563117 3564007 3 891 TM.orf3239 cyaA putativeadenylate/guanylate cyclase 3566353 3564008 −3 2346 TM.orf3240 FPGS FolCbifunctional protein 3567653 3566331 −2 1323 TM.orf3241 trxA thioredoxin1 3568048 3567725 −3 324 TM.orf3242 addA UvrD/REP helicase 35717043568213 −1 3492 TM.orf3243 conserved hypothetical protein 35747963571701 −2 3096 TM.orf3244 nucleotidyl transferase 3575568 3574801 −1768 TM.orf3245 aminoglycoside phosphotransferase 3576644 3575565 −2 1080TM.orf3246 ATP-binding protein RP013 3577213 3576641 −3 573 TM.orf3247ffh signal recognition particle, subunit SRP54 3577543 3578898 1 1356TM.orf3248 rpsP 30S ribosomal protein S16 3578997 3579362 2 366TM.orf3249 rimM 50S ribosomal protein L19 3579412 3579993 1 582TM.orf3250 trmD tRNA (guanine-N1)-methyltransferase 3579990 3580757 2768 TM.orf3251 rplS 50S ribosomal protein L19 3580972 3581352 1 381TM.orf3252 leuC 3-isopropylmalate/(R)-2-methylmalate 3581677 3583095 11419 dehydratase large subunit TM.orf3253 leuD 3-isopropylmalatedehydratase small subunit 3583134 3583751 2 618 TM.orf3254 leuBIsocitrate/isopropylmalate dehydrogenase 3583878 3584987 2 1110TM.orf3255 asd Aspartate-semialdehyde dehydrogenase, 3585223 3586281 11059 USG-1 TM.orf3256 oxidoreductase domain protein 3586295 3586954 3660 TM.orf3257 fabG putative 3-oxoacyl- 3587684 3586980 −2 705TM.orf3258 trap dicarboxylate transporter- dctp subunit 3587917 35890411 1125 TM.orf3259 TRAP-T family transporter, DctQ (4 TMs) 35890693589629 1 561 subunit TM.orf3260 siaT trap-t family transporter, dctm(12 tms) subunit 3589629 3590936 2 1308 TM.orf3261 transcriptionalregulator, TetR family protein 3591010 3591693 1 684 TM.orf3262enoyl-CoA hydratase 3591720 3592520 2 801 TM.orf3263 CoA-binding protein3592517 3593758 3 1242 TM.orf3264 GNAT family acetyltransferase 35938753594702 1 828 TM.orf3265 yngJ putative acyl-CoA dehydrogenase yngJ3594699 3595880 2 1182 TM.orf3266 yecA amino acid transporter 35980083596713 −1 1296 TM.orf3267 Cupin domain protein 3598343 3598005 −2 339TM.orf3268 ycgG conserved hypothetical protein 3599129 3598347 −2 783TM.orf3269 lysM transcription regulator 3599342 3599839 3 498 TM.orf3270pdxY pyridoxamine kinase 3600724 3599858 −3 867 TM.orf3271 citE citratelyase beta subunit 3601172 3602044 3 873 TM.orf3272 Acyl dehydratase3602055 3603122 2 1068 TM.orf3273 sdhC succinate dehydrogenasecytochrome b-556 3603352 3603738 1 387 subunit TM.orf3274 sdhD succinatedehydrogenase, hydrophobic 3603788 3604168 3 381 membrane anchor proteinTM.orf3275 sdhA succinate dehydrogenase flavoprotein subunit 36041763605966 2 1791 TM.orf3276 sdhB succinate dehydrogenase iron-sulfursubunit 3606010 3606783 1 774 TM.orf3277 ygaD ABC transporterATP-binding/permease 3608760 3607006 −1 1755 TM.orf3278 ABC transporterrelated protein 3610532 3608757 −2 1776 TM.orf3279 major facilitatortransporter 3611773 3610529 −3 1245 TM.orf3280 fyuA TonB-dependentreceptor 3613947 3611788 −1 2160 TM.orf3281 pchR helix-turn-helixdomain-containing protein 3614138 3615079 3 942 TM.orf3282 hypotheticalprotein 3614145 3613993 −1 153 TM.orf3283 urease-associated protein3615596 3615351 −2 246 TM.orf3284 yegD chaperone protein yegD 36160923617390 2 1299 TM.orf3285 alanine racemase domain-containing protein3618219 3617413 −1 807 TM.orf3286 HPr kinase 3619309 3618341 −3 969TM.orf3287 conserved hypothetical protein 3619452 3620324 2 873TM.orf3288 WbuT 3621042 3620362 −1 681 TM.orf3289 hypothetical protein3621383 3621243 −2 141 TM.orf3290 glycosyl transferase family 2 36223693621461 −3 909 TM.orf3291 conserved hypothetical protein 3622716 3622423−1 294 TM.orf3292 conserved hypothetical protein 3623628 3622744 −1 885TM.orf3293 extradiol ring-cleavage dioxygenase III subunit B 36245323623735 −3 798 TM.orf3294 DoxD-like family protein 3625025 3624582 −2444 TM.orf3295 nahR transcriptional regulator MexT 3625214 3626170 3 957TM.orf3296 isomerase 3626969 3626145 −2 825 TM.orf3297 short-chaindehydrogenase/reductase SDR 3627802 3626993 −3 810 TM.orf3298 CoxK3628846 3627869 −3 978 TM.orf3299 pecT HTH-type transcriptionalregulator 3629067 3630047 2 981 TM.orf3300 kefC potassium efflux systemprotein 3630112 3631839 1 1728 TM.orf3301 icfA Carbonate dehydratase3632556 3631864 −1 693 TM.orf3302 ugpQ glycerophosphoryl diesterphosphodiesterase 3632751 3633533 2 783 TM.orf3303 ugpB extracellularsolute-binding protein, family 1 3633629 3634957 3 1329 TM.orf3304 ugpASN-glycerol-3-phosphate ABC transporter, 3635054 3635941 3 888 permeaseprotein TM.orf3305 ugpE sn-glycerol-3-phosphate ABC transporter, 36359573636811 3 855 permease protein TM.orf3306 ugpC glycerol-3-phosphatetransporter ATP-binding 3637061 3638218 3 1158 subunit TM.orf3307 mscSMscS mechanosensitive ion channel 3639138 3638236 −1 903 TM.orf3308 TM23639549 3639205 −1 345 TM.orf3309 2-nitropropane dioxygenase NPD 36401123639657 −2 456 TM.orf3310 putative nitropropane dioxygenase 36407653640112 −3 654 TM.orf3311 gitR regulatory protein, LysR: LysR,substrate- 3640840 3641739 1 900 binding TM.orf3312phospholipase/carboxylesterase 3642450 3641746 −1 705 TM.orf3313phospholipase/carboxylesterase 3643159 3642488 −3 672 TM.orf3314 F-boxprotein SKIP8 3643373 3643801 3 429 TM.orf3315 multiple antibioticresistance (MarC)-related 3643901 3644626 3 726 protein TM.orf3316 ppxExopolyphosphatase 3646169 3644631 −2 1539 TM.orf3317 ppk Polyphosphatekinase 3648626 3646239 −2 2388 TM.orf3318 putative phosphohistidinephosphatase, SixA 3648772 3649290 1 519 TM.orf3319 antibioticbiosynthesis monooxygenase 3649628 3649284 −2 345 TM.orf3320 Glycinehydroxymethyltransferase 3650926 3649625 −3 1302 TM.orf3321 argDacetylornithine and succinylornithine 3652146 3650962 −1 1185 TM.orf3322benE benzoate transporter 3653378 3652143 −2 1236 TM.orf3323 mocR GntRfamily transcriptional regulator 3653578 3655107 1 1530 TM.orf3324 Gde1glycerophosphodiester phosphodiesterase 3656230 3655403 −3 828TM.orf3325 yfiQ CoA-binding domain protein 3658988 3656235 −2 2754TM.orf3326 HAD-superfamily subfamily IIA hydrolase, 3659981 3659109 −2873 hypothetical 3: HAD-superfamily hydrolase, subfamily IIA TM.orf3327diguanylate phosphodiesterase 3661334 3660045 −2 1290 TM.orf3328 pyrDDihydroorotate dehydrogenase 3662385 3661303 −1 1083 TM.orf3329conserved hypothetical protein 3662783 3662382 −2 402 TM.orf3330 argEacetylornithine deacetylase (ArgE) 3664125 3662839 −1 1287 TM.orf3331trkH trk system potassium uptake protein TrkH 3665726 3664275 −2 1452TM.orf3332 folE GTP cyclohydrolase I 3666626 3665961 −2 666 TM.orf3333apaG ApaG 3667368 3666961 −1 408 TM.orf3334 metZ O-succinylhomoserinesulfhydrylase 3668810 3667530 −2 1281 TM.orf3335 ynjD ABC transporterrelated protein 3669666 3669016 −1 651 TM.orf3336 ynjC inner membraneABC transporter permease 3671393 3669663 −2 1731 protein YnjC TM.orf3337ynjB ABC transporter, periplasmic binding protein 3672705 3671401 −11305 TM.orf3338 hypothetical protein 3673748 3672804 −2 945 TM.orf3339traG TRAG family protein 3675901 3673769 −3 2133 TM.orf3340 hypotheticalprotein 3676961 3676122 −2 840 TM.orf3341 icd isocitrate dehydrogenase3678798 3677308 −1 1491 TM.orf3342 yhdG putative transmembrane permeaseprotein 3679162 3680625 1 1464 TM.orf3343 maoI tyramine oxidase 36807023682624 3 1923 TM.orf3344 hypothetical protein 3682673 3683380 3 708TM.orf3345 virS AraC family transcriptional regulator 3683558 3684568 31011 TM.orf3346 xthA exonuclease III 3684652 3685458 1 807 TM.orf3347gsiB extracellular solute-binding protein, family 5 3686923 3685520 −31404 TM.orf3348 hslU ATP-dependent protease ATP-binding subunit 36885373687224 −3 1314 HslU TM.orf3349 hslV ATP-dependent protease peptidasesubunit 3689097 3688537 −1 561 TM.orf3350 conserved hypothetical protein3690866 3689211 −2 1656 TM.orf3351 ycaC isochorismatase hydrolase3691676 3690963 −2 714 TM.orf3352 cpo putative haloperoxidase protein3692564 3691734 −2 831 TM.orf3353 HPA3 GCN5-related N-acetyltransferase3693508 3693056 −3 453 TM.orf3354 yijE inner membrane transporter yiJE3694436 3693513 −2 924 TM.orf3355 acetyltransferase protein 36951553694433 −3 723 TM.orf3356 mocR aminotransferase protein 3695271 36966742 1404 TM.orf3357 dgdR LysR family transcriptional regulator 36975943696671 −3 924 TM.orf3358 hisB imidazoleglycerol-phosphate dehydratase3697747 3698352 1 606 TM.orf3359 hypothetical protein 3698393 3698851 3459 TM.orf3360 hisH imidazole glycerol phosphate synthase subunit3698993 3699637 3 645 hisH TM.orf3361 hisAphosphoribosylformimino-5-aminoimidazole 3699708 3700439 2 732carboxamide ribotide TM.orf3362 hisF imidazole glycerol phosphatesynthase subunit 3700494 3701261 2 768 HisF TM.orf3363 hisIphosphoribosyl-AMP 3701377 3701835 1 459cyclohydrolase/phosphoribosyl-ATP pyrophosphohydrolase TM.orf3364 hisEphosphoribosyl-ATP pyrophosphohydrolase 3701816 3702190 3 375 TM.orf3365conserved hypothetical protein 3702304 3702672 1 369 TM.orf3366 Membraneflavodoxin oxidoreductase 3702745 3704115 1 1371 TM.orf3367 dgoK2-dehydro-3-deoxygalactonokinase 3704189 3705103 3 915 TM.orf3368 dgoA2-dehydro-3-deoxy-6-phosphogalactonate 3705161 3705793 3 633 aldolaseTM.orf3369 conserved hypothetical protein 3706414 3705794 −3 621TM.orf3370 conserved hypothetical protein 3707291 3706407 −2 885TM.orf3371 mauG methylamine utilization protein 3708680 3707562 −2 1119TM.orf3372 conserved hypothetical protein 3709534 3708677 −3 858TM.orf3373 hemR iron complex outermembrane recepter protein 37116263709548 −2 2079 TM.orf3374 conserved hypothetical protein 37122143711762 −2 453 TM.orf3375 conserved hypothetical protein 3712816 3712292−3 525 TM.orf3376 hypothetical protein 3713156 3712809 −2 348 TM.orf3377transcriptional regulator, AraC family 3714267 3713281 −1 987 TM.orf3378nepI major facilitator superfamily MFS_1 3714406 3715620 1 1215TM.orf3379 Sugar kinases, ribokinase family 3716470 3715598 −3 873TM.orf3380 HTH-type transcriptional regulator 3716902 3716489 −3 414TM.orf3381 Smr protein/MutS2 3717771 3716899 −1 873 TM.orf3382 conservedhypothetical protein 3718475 3717780 −2 696 TM.orf3383 lutB Iron-sulfurcluster binding protein 3719953 3718472 −3 1482 TM.orf3384 putative Fe—Soxidoreductase 3720788 3719970 −2 819 TM.orf3385 mltA membrane-boundlytic murein 3722192 3720909 −2 1284 transglycosylase A TM.orf3386cytochrome P450 3723432 3722260 −1 1173 TM.orf3387 prenyltransferase andsqualene oxidase 3725420 3723429 −2 1992 repeat domain proteinTM.orf3388 HPr(Ser) kinase/phosphatase 3726331 3725417 −3 915 TM.orf3389asnO Asparagine synthase (glutamine-hydrolyzing) 3728229 3726328 −1 1902TM.orf3390 conserved hypothetical protein 3728504 3728229 −2 276TM.orf3391 hypothetical protein 3728685 3728542 −1 144 TM.orf3392 bioIcytochrome P450 enzyme 3729760 3729026 −3 735 TM.orf3393 hypotheticalprotein 3730089 3729778 −1 312 TM.orf3394 conserved hypothetical protein3730769 3730086 −2 684 TM.orf3395 ytzA FxsA 3730977 3731501 2 525TM.orf3396 secB preprotein translocase subunit SecB 3731642 3732124 3483 TM.orf3397 yhjC LysR family transcriptional regulator 37331213732210 −2 912 TM.orf3398 padA NmrA family protein 3733216 3734100 1 885TM.orf3399 class D beta-lactamase 3734157 3735041 2 885 TM.orf3400 dnaQDNA polymerase III, epsilon subunit and 3735732 3735046 −1 687 related3′-5′ exonuclease TM.orf3401 coaE Dephospho-CoA kinase 3736443 3735775−1 669 TM.orf3402 aroE shikimate 5-dehydrogenase 3737348 3736437 −2 912TM.orf3403 Nucleotide-binding protein implicated in 3737986 3737348 −3639 inhibition of septum formation TM.orf3404 phosphotransferase 37389443737991 −2 954 TM.orf3405 hemE uroporphyrinogen decarboxylase 37396973740749 3 1053 TM.orf3406 hemH Protoheme ferro-lyase 3740758 3741849 11092 TM.orf3407 membrane protein 3741890 3742336 3 447 TM.orf3408 rhotranscription termination factor Rho 3743021 3744280 3 1260 TM.orf3409qor NADPH: quinone reductase and related Zn- 3745374 3744400 −1 975dependent oxidoreductase TM.orf3410 carboxymethylenebutenolidase 37462633745568 −3 696 TM.orf3411 ogt methylated-DNA- 3746941 3746366 −3 576TM.orf3412 yyaL conserved hypothetical protein 3749004 3746938 −1 2067TM.orf3413 conserved hypothetical protein 3749135 3749389 3 255TM.orf3414 fdx4 ferredoxin, 2Fe—2S 3749397 3749807 2 411 TM.orf3415 mnmEtRNA modification GTPase TrmE 3749964 3751349 2 1386 TM.orf3416 mnmGtRNA uridine 5-carboxymethylaminomethyl 3751553 3753502 3 1950modification enzyme GidA TM.orf3417 rsmG S-adenosylmethionine-dependent3753508 3754236 1 729 methyltransferase involved in cell divisionTM.orf3418 parA chromosome segregation ATPase 3754208 3755032 3 825TM.orf3419 parB chromosome partitioning protein parB 3755029 3755961 1933 TM.orf3420 DNA polymerase III, delta subunit 3757059 3756025 −1 1035TM.orf3421 LPS-assembly lipoprotein 3757593 3757075 −1 519 TM.orf3422leuS leucyl-tRNA synthetase 3760225 3757640 −3 2586 TM.orf3423 conservedhypothetical protein 3760903 3760355 −3 549 TM.orf3424 Outer membraneprotein (porin) 3761439 3762518 2 1080 TM.orf3425 Outer membrane protein(porin) 3762847 3763941 1 1095 TM.orf3426 Outer membrane protein (porin)3764382 3765479 2 1098 TM.orf3427 sulfotransferase 3765670 3766569 1 900TM.orf3428 conserved hypothetical protein 3766566 3767432 2 867TM.orf3429 putative enzyme with PLP-binding domain 3768118 3768804 1 687alanine racemase TM.orf3430 conserved hypothetical protein 37693183768809 −3 510 TM.orf3431 conserved hypothetical protein 3770663 3769416−2 1248 TM.orf3432 ribA GTP cyclohydrolase II 3772029 3770914 −1 1116TM.orf3433 YceI family protein 3772739 3772092 −2 648 TM.orf3434CDP-alcohol phosphatidyltransferase 3773527 3772739 −3 789 TM.orf3435deaminase-reductase domain-containing 3773762 3774832 3 1071 proteinTM.orf3436 kanE FAD dependent oxidoreductase 3774829 3775785 1 957TM.orf3437 conserved hypothetical protein 3775834 3776253 1 420TM.orf3438 putative glycosyl transferase 3776268 3777335 2 1068TM.orf3439 Methyltransferase type 12 3777335 3778162 3 828 TM.orf3440sphR two component transcriptional regulator 3778236 3778937 2 702TM.orf3441 nahR transcriptional regulator, LysR family 3779852 3778944−2 909 TM.orf3442 ThiJ/PfpI domain protein 3779976 3780632 2 657TM.orf3443 hypothetical protein 3779977 3779864 −3 114 TM.orf3444 fadBenoyl-CoA hydratase/isomerase 3781441 3780680 −3 762 TM.orf3445transcriptional regulators 3782264 3781557 −2 708 TM.orf3446 yifBPredicted ATPase with chaperone activity 3783896 3782283 −2 1614TM.orf3447 conserved hypothetical protein 3785042 3784608 −2 435TM.orf3448 nth endonuclease III 3785229 3785960 2 732 TM.orf3449conserved hypothetical protein 3786067 3786216 1 150 TM.orf3450 ppainorganic pyrophosphatase 3787457 3786930 −2 528 TM.orf3451 ampG majorfacilitator superfamily permease 3788915 3787551 −2 1365 TM.orf3452 typAGTP-binding protein TypA/BipA 3790767 3788941 −1 1827 TM.orf3453 Sugarkinase 3791831 3790839 −3 993 TM.orf3454 conserved hypothetical protein3792175 3792870 2 696 TM.orf3455 hypothetical protein 3792902 3793315 1414 TM.orf3456 tetratricopeptide TPR_4 3794296 3793322 −1 975 TM.orf3457type I phosphodiesterase/nucleotide 3795910 3794378 −1 1533pyrophosphatase TM.orf3458 yihL putative transcriptional regulator3796104 3796805 3 702 TM.orf3459 flhA alcohol dehydrogenase class III3796872 3797984 3 1113 TM.orf3460 Esd intracellular septation protein A3797989 3798858 2 870 TM.orf3461 hypothetical protein 3799428 3798883 −2546 TM.orf3462 arsC putative arsenate reductase 3799966 3799613 −1 354TM.orf3463 gshB glutathione synthetase 3800927 3799986 −3 942 TM.orf3464tetratricopeptide repeat family protein 3801935 3801054 −3 882TM.orf3465 Predicted periplasmic or secreted lipoprotein 3802603 3801932−1 672 TM.orf3466 conserved hypothetical protein 3803051 3802647 −3 405TM.orf3467 uroporphyrin-III C/tetrapyrrole 3804199 3803048 −1 1152(corrin/porphyrin) methyltransferase TM.orf3468 Extracellularligand-binding receptor 3804283 3805578 2 1296 TM.orf3469coproporphyrinogen III oxidase 3806806 3805607 −1 1200 TM.orf3470Xanthosine triphosphate pyrophosphatase 3807426 3806815 −2 612TM.orf3471 rph RNase PH 3808418 3807441 −3 978 TM.orf3472 hrcATranscriptional regulator of heat shock gene 3808725 3809855 3 1131TM.orf3473 grpE GrpE protein 3810026 3810646 1 621 TM.orf3474 dnaKmolecular chaperone DnaK 3810999 3812927 3 1929 TM.orf3475 dnaJchaperone protein DnaJ 3813080 3814261 1 1182 TM.orf3476 dapBdihydrodipicolinate reductase 3814452 3815207 3 756 TM.orf3477 yedAinner membrane transporter yedA 3816161 3815238 −3 924 TM.orf3478 phbCPoly-beta-hydroxybutyrate polymerase domain 3818234 3816252 −3 1983protein TM.orf3479 hypothetical protein 3818477 3818340 −3 138TM.orf3480 yhiI HlyD family secretion protein 3818514 3819494 3 981TM.orf3481 yhiH ABC transporter, ATP binding/permease 3819491 3822256 12766 protein TM.orf3482 yhhJ Inner membrane transport permease yhhJ3822261 3823385 3 1125 TM.orf3483 hipA HipA domain protein 38248203823498 −2 1323 TM.orf3484 hypothetical protein 3824978 3824820 −3 159TM.orf3485 icc putative ICC protein 3826129 3825308 −1 822 TM.orf3486potA putative ABC transporter 3827137 3826136 −1 1002 TM.orf3487 modBbinding-protein-dependent transport systems 3827937 3827134 −2 804 innermembrane component TM.orf3488 cysW putative permease protein 38287763827943 −3 834 TM.orf3489 family 1 extracellular solute-binding protein3829893 3828871 −2 1023 TM.orf3490 purR regulatory protein 38310303829978 −2 1053 TM.orf3491 mviN virulence factor 3832713 3831157 −2 1557TM.orf3492 glnD [protein-PII] uridylyltransferase 3835601 3832800 −32802 TM.orf3493 metQ YaeC family lipoprotein 3836668 3835802 −1 867TM.orf3494 metQ D-methionine ABC superfamily ATP binding 3837713 3836868−3 846 cassette transporter, binding protein TM.orf3495 mcp3methyl-accepting chemotaxis sensory 3839287 3837932 −1 1356 transducerTM.orf3496 esterase/lipase/thioesterase 3840277 3839309 −1 969TM.orf3497 virulence protein 3841146 3840379 −2 768 TM.orf3498 mprFputative membrane protein 3843692 3841143 −3 2550 TM.orf3499 branchedchain amino acid ABC transporter 3845162 3843942 −3 1221 aminoacid-binding protein TM.orf3500 alkK medium-chain-fatty-acid-CoA ligase3845541 3847160 3 1620 TM.orf3501 mutS DNA mismatch repair protein3849952 3847139 −1 2814 TM.orf3502 tme malic enzyme 3850401 3852698 32298 TM.orf3503 phoR two-component sensor histidine kinase 38529143854356 1 1443 TM.orf3504 lcfB acyl-CoA synthase 3854482 3856020 2 1539TM.orf3505 sphX phosphate ABC transporter, periplasmic 3856419 3857441 31023 binding protein TM.orf3506 yqgH phosphate ABC transporter, permeaseprotein 3857697 3859079 3 1383 PstC TM.orf3507 yqgI phosphate ABCtransporter, inner membrane 3859099 3860424 2 1326 subunit PstATM.orf3508 Phosphate import ATP-binding protein 3860520 3861290 3 771TM.orf3509 phoU Phosphate transport system protein PhoU 3861318 38620433 726 TM.orf3510 phoB Response regulator 3862169 3862867 1 699TM.orf3511 rhtB lysine exporter protein LysE/YggA 3862963 3863589 2 627TM.orf3512 trpS Tryptophanyl-tRNA synthetase 3863762 3864754 1 993TM.orf3513 putative permease 3864903 3865814 3 912 TM.orf3514 conservedhypothetical protein 3865814 3866596 1 783 TM.orf3515 universal stressfamily protein 3866612 3867124 1 513 TM.orf3516 cya1 adenylate cyclase3868421 3867117 −3 1305 TM.orf3517 soxR Redox-sensitive transcriptionalactivator soxR 3869016 3868549 −2 468 TM.orf3518 conserved hypotheticalprotein 3869119 3869538 2 420 TM.orf3519 RND efflux membrane fusionprotein precursor 3869543 3870643 1 1101 TM.orf3520 RND effluxtransporter 3870640 3873729 2 3090 TM.orf3521 thioredoxin 38738143874359 2 546 TM.orf3522 Diacylglycerol kinase, catalytic region 38766933875188 −2 1506 oxidoreductase, short chain TM.orf3523 fabGdehydrogenase/reductase family 3877381 3876680 −1 702 TM.orf3524 ddpXD-alanyl-D-alanine dipeptidase 3877980 3877423 −2 558 TM.orf3525 yeaZpeptidase M22, glycoprotease 3878054 3878785 1 732 TM.orf3526ribosomal-protein-alanine acetyltransferase 3878782 3879258 2 477TM.orf3527 transcriptional regulatory protein 3879811 3880233 2 423TM.orf3528 fur ferric uptake regulator family protein 3880551 3880997 3447 TM.orf3529 hemolysin 3881199 3881978 3 780 TM.orf3530 plsC1-acyl-sn-glycerol-3-phosphate 3881975 3882907 1 933 acyltransferaseTM.orf3531 miaB RNA modification protein 3883012 3884415 2 1404TM.orf3532 ybeZ phosphate starvation-inducible protein PhoH 38844573885599 3 1143 and related proteins TM.orf3533 Predicted metal-dependenthydrolase 3885596 3886255 1 660 TM.orf3534 CBS domain-containing protein3886567 3887403 2 837 TM.orf3535 lnt Apolipoprotein N-acyltransferase3887442 3889046 3 1605 TM.orf3536 transcriptional regulator 38893523889789 1 438 TM.orf3537 metK S-adenosylmethionine synthetase 38900913891260 3 1170 TM.orf3538 trmB tRNA (guanine-N7-)-methyltransferase3891360 3892103 3 744 TM.orf3539 rimP Ribosome maturation factor rimP3892428 3892922 3 495 TM.orf3540 nusA transcription elongation factorNusA 3892931 3894490 1 1560 TM.orf3541 conserved hypothetical protein3894501 3895187 3 687 TM.orf3542 infB translation initiation factor IF-23895198 3898329 2 3132 TM.orf3543 rbfA ribosome-binding factor A 38984313898880 3 450 TM.orf3544 truB Pseudouridine synthase 3898885 3899802 2918 TM.orf3545 rpsO ribosomal protein S15 3899820 3900089 3 270TM.orf3546 pnp polyribonucleotide nucleotidyltransferase 3900584 39028301 2247 TM.orf3547 Dioxygenases related to 2-nitropropane 3903207 39045563 1350 dioxygenase TM.orf3548 fur Fe2+/Zn2+ uptake regulation proteins3904706 3905116 1 411 TM.orf3549 rbr Rubrerythrin 3905178 3905681 3 504TM.orf3550 conserved hypothetical protein 3906332 3905733 −3 600TM.orf3551 gyaR lactate dehydrogenase and related 3906637 3907617 2 981dehydrogenase TM.orf3552 conserved hypothetical protein 3907757 39084821 726 TM.orf3553 conserved hypothetical protein 3908952 3908497 −2 456TM.orf3554 molybdopterin biosynthesis protein 3909780 3908965 −2 816TM.orf3555 cysK cysteine synthase A 3910819 3909845 −1 975 TM.orf3556iscR transcriptional regulator 3911327 3910872 −3 456 TM.orf3557 dutdeoxyuridine 5′triphosphate 3911860 3911402 −1 459 nucleotidohydrolaseprotein TM.orf3558 phosphopantothenoylcysteine 3913134 3911860 −2 1275decarboxylase/phosphopantothenate--cysteine ligase TM.orf3559 ubiBprotein kinase 3914820 3913306 −2 1515 TM.orf3560 ubiE2-octaprenyl-6-methoxy-1,4-benzoquinone 3915654 3914848 −2 807methylase/demethylmenaquinone methyltransferase TM.orf3561 mutMformamidopyrimidine-DNA glycosylase 3915740 3916603 1 864 TM.orf3562conserved hypothetical protein 3916600 3917028 2 429 TM.orf3563enoyl-CoA hydratase/isomerase family protein 3917177 3917950 1 774TM.orf3564 rpsT Ribosomal protein S20p 3918135 3918404 3 270 TM.orf3565rhodanese domain-containing protein 3918958 3919428 2 471

Example 4 Conjugation of Tistrella mobilis Plasmid

Conjugation refers to the transfer of a plasmid from one bacterial cellto another. During conjugation, the bacterial cell that harbors theplasmid is regarded as the donor cell and the other cell lacking theplasmid is regarded as recipient cells. The donor cell must have a setof genes that are capable of doing two functions: 1) production of thesex pilus in which two bacterial cells are brought together and throughwhich the plasmid DNA is transferred to the recipient cell, and 2) theDNA-processing component that processes the plasmid at a particular siteso that a single strand of the plasmid is unwound and transferred to therecipient cell. The sex pilus in most Gram-negative bacteria is encodedby a set of 11 genes with conserved sequences and functions. Tistrellamobilis, being Gram-negative, is found to harbor the same set of geneson its chromosomal DNA. The corresponding 11 homologous genes can befound from orf TM.2187-2197, which in certain embodiments indicates thecapability of T. mobilis to produce the sex pilus required for theconjugation process. Furthermore, plasmid 3, which harbors the didemningene cluster, is found to encode several genes that in some embodimentsare involved in the DNA processing of the plasmid. Among them,P3.orf0026, encodes a relaxase, a central component for DNA processing,which further implies that plasmid 3 may be transferred to another cellby conjugation. Other nearby sequences of the relaxase also encode someother genes that are involved in the conjugation. For example,P3.orf0056 encodes a lytic transglycosylase, and it is believed todegrade bacterial cell wall for DNA transfer during conjugation.

Therefore, in specific embodiments plasmid 3 in which the didemnin genecluster resides may transfer to another bacterial species. Other speciesin the genus of Tistrella could be candidates in embodiments whereinthey do not have the same plasmid. Species from closely related genera,such as members of Azospirillum and Agrobacteria (for example only), maybe recipients, in certain embodiments.

REFERENCES

All patents and publications mentioned in the specification areindicative of the level of those skilled in the art to which theinvention pertains. All patents and publications are herein incorporatedby reference in their entirety to the same extent as if each individualpublication was specifically and individually indicated to beincorporated by reference.

PATENTS

-   -   U.S. Pat. No. 5,294,603    -   U.S. Pat. No. 5,399,363    -   U.S. Pat. No. 5,466,468    -   U.S. Pat. No. 5,543,158    -   U.S. Pat. No. 5,580,579    -   U.S. Pat. No. 5,629,001    -   U.S. Pat. No. 5,641,515    -   U.S. Pat. No. 5,792,451    -   U.S. Pat. No. 6,613,308    -   U.S. Pat. No. 6,841,530

PUBLICATIONS

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Although the present invention and its advantages have been described indetail, it should be understood that various changes, substitutions andalterations can be made herein without departing from the spirit andscope of the invention as defined by the appended claims. Moreover, thescope of the present application is not intended to be limited to theparticular embodiments of the process, machine, manufacture, compositionof matter, means, methods and steps described in the specification. Asone of ordinary skill in the art will readily appreciate from thedisclosure of the present invention, processes, machines, manufacture,compositions of matter, means, methods, or steps, presently existing orlater to be developed that perform substantially the same function orachieve substantially the same result as the corresponding embodimentsdescribed herein may be utilized according to the present invention.Accordingly, the appended claims are intended to include within theirscope such processes, machines, manufacture, compositions of matter,means, methods, or steps.

1. An isolated Tistrella mobilis bacterium having Accession DepositNumber NRRL B-50531 with the depository Agricultural Research ServiceCulture Collection National Center for Agricultural Utilization ResearchAgricultural Research Service, U.S. Department of Agriculture.
 2. Anisolated polynucleotide selected from the group consisting of SEQ IDNO:10-25, 42-51, 62-66, and a mixture thereof.
 3. An isolatedpolypeptide selected from the group consisting of SEQ ID NO:26-41,52-61, and a mixture thereof.
 4. The bacterium of claim 1, wherein thebacterium comprises at least one genetic modification compared towild-type.
 5. The bacterium of claim 4, wherein the genetic modificationis in a gene in the didemnin gene cluster.
 6. The bacterium of claim 4,wherein the at least one genetic modification is in a condensation,adenylation, thiolation, ketoreductase, ketosynthase, methyltransferase,or thioesterase domain of a gene in the didemnin gene cluster.
 7. Thebacterium of claim 6, wherein the at least one genetic modification isin the ketoreductase or adenylation domains.
 8. A method of producing adidemnin precursor, didemnin, or didemnin derivative, comprising thesteps of: a) culturing a host cell harboring bacterial didemninsynthesis genes; and b) recovering said didemnin precursor, didemnin, ordidemnin derivative from said host cell.
 9. The host cell of claim 8,further defined as a Tistrella bacterium, a genetically modifiedTistrella bacterium compared to wild-type, or E. coli.
 10. The method ofclaim 9, wherein the Tistrella bacterium is Tistrella mobilis.
 11. Themethod of claim 8, wherein the didemnin is selected from the groupconsisting of didemnin A, didemnin B, didemnin C, didemnin D, didemninE, didemnin G, didemnin X, didemnin Y, nordidemnin, or a combinationthereof.
 12. The method of claim 8, further comprising modifying saidrecovered didemnin precursor, didemnin, or didemnin derivative.
 13. Atherapeutic composition comprising in a suitable carrier: at least oneisolated didemnin produced by a culture comprising bacteria havingAccession Deposit Number NRRL B-50531 with the depository AgriculturalResearch Service Culture Collection National Center for AgriculturalUtilization Research Agricultural Research Service, U.S. Department ofAgriculture; at least one didemnin or didemnin derivative produced froma modified bacteria of the bacterial strain having Accession DepositNumber NRRL B-50531 with the depository Agricultural Research ServiceCulture Collection National Center for Agricultural Utilization ResearchAgricultural Research Service, U.S. Department of Agriculture; or amixture thereof.